Abstract:
:Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis biofilm has received less attention. This study aims to evaluate the effect of different growth conditions on the characteristics of E. faecalis biofilm on root canal, and the penetration of E. faecalis into dentinal tubules. Forty-five intact noncarious human maxillary molars were experimented under nutrient-rich, nutrient-deprived, aerobic, and anaerobic conditions for a period of 21 days. Scanning Electron Microscopy with Energy Dispersive X-ray microanalysis, Laser Confocal Scanning Microscopy and Light microscopic examinations were carried out. The microscopic analysis highlighted a distinct variation in the ultrastructure of the biofilms formed under different experimental conditions. The EDX microanalysis showed a significant increase in the levels of Calcium (Ca) in the biofilm structures formed under anaerobic nutrient-deprived condition (p < 0.001). The depth of bacterial penetration was significantly greater in nutrient-rich condition (p < 0.001). This study demonstrated distinct ultrastructural and physiochemical properties of the biofilms formed and dentinal tubular penetration of E. faecalis under different conditions.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
George S,Kishen A,Song KPdoi
10.1097/01.don.0000164855.98346.fckeywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
867-72issue
12eissn
0099-2399issn
1878-3554pii
S0099-2399(06)61110-2journal_volume
31pub_type
杂志文章abstract:INTRODUCTION:Instrumentation of the mesial root canal system of mandibular molars may hinder disinfection by packing hard tissue debris within the isthmuses. The removal of accumulated hard tissue debris (AHTD) by 3 supplemental irrigation systems, 2 ultrasonically activated and 1 multisonic, was assessed with micro-co...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.013
更新日期:2019-07-01 00:00:00
abstract::The aim of this review was to consider laboratory experiments and clinical studies of calcium hydroxide-based root canal sealers. An extensive search of the endodontic literature was made to identify publications related to calcium hydroxide-based root canal sealers. The articles were assessed for the outcome of labor...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2008.11.026
更新日期:2009-04-01 00:00:00
abstract::The sealing ability of copal varnish has been investigated when used as an adjunct in restorative procedures, retrofilling procedures, and dontic obturation procedures. Barrier, a polyamide-type polymer cavity varnish, has been compared with copal varnishes in restorative dentistry. This investigation compared the api...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81138-6
更新日期:1992-01-01 00:00:00
abstract::Root resorption after replantation of mature permanent dog incisors was examined by scanning electron microscopy. Three groups consisting of three extracted teeth each were kept in a saline solution for 5, 30, and 120 min before replantation. The first group was replanted with the pulp intact. The second and third gro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80996-9
更新日期:1995-05-01 00:00:00
abstract::The 4- to 6-yr outcome of initial (first-time) endodontic treatment was assessed for Phase II of the "Toronto Study." In total, 442 teeth were treated by using flared preparation and vertical compaction of warm gutta-percha or step-back preparation and lateral compaction. With 126 teeth excluded (discontinuers: deceas...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200405000-00002
更新日期:2004-05-01 00:00:00
abstract:INTRODUCTION:We aimed to compare the antimicrobial efficacy of chlorhexidine (CHX) and sodium hypochlorite (NaOCl), 2 irrigants routinely used in root canal therapy of permanent teeth. METHODS:Electronic databases, including PubMed, EMBASE, Web of Science, and Cochrane Library, were searched for randomized controlled ...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2020.05.002
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:Incision and drainage of symptomatic emergency patients with facial swelling is painful even after local anesthetics are administered. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and the pain of an incision and drainage procedure of a buffered ver...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.06.017
更新日期:2015-10-01 00:00:00
abstract:INTRODUCTION:Calcium hydroxide (CH) and nano-calcium hydroxide (NCH) as intracanal medicaments may affect the physical and chemical properties of dentin. The aim of this investigation was to evaluate the effects of CH and NCH on the microhardness and superficial chemical structure of radicular dentin. METHODS:In this ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.06.002
更新日期:2019-09-01 00:00:00
abstract:INTRODUCTION:This study aimed to compare the success rates of direct pulp capping (DPC) by using either ProRoot Mineral Trioxide Aggregate (MTA) or Biodentine in the cariously exposed permanent teeth of 6- to 18-year-old patients. Gray discoloration was also evaluated. METHODS:Fifty-nine cariously exposed permanent te...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2017.10.012
更新日期:2018-03-01 00:00:00
abstract:INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.026
更新日期:2010-04-01 00:00:00
abstract::The aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: Facultative aerobes-anaerobes: Candida albicans ATCC 10231; Enterococcus faecalis ATCC 29212; Escherichia coli ATCC 25,922; Pseudomonas aeruginosa ATCC 27,85...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81170-2
更新日期:1999-05-01 00:00:00
abstract::Simulated furcation perforations were repaired with and without blood contamination utilizing tooth-colored mineral trioxide aggregrate (MTA) mixed with either MTA liquid (sterile water), lidocaine, or saline. Samples underwent Instron testing at either 24 or 72 hours and at 7 days. Data were analyzed using a 3-way AN...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.09.007
更新日期:2006-05-01 00:00:00
abstract::EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200112000-00007
更新日期:2001-12-01 00:00:00
abstract:INTRODUCTION:Dentin regeneration is one of the main goals of vital pulp treatment in which the biological properties of dental pulp cells (DPCs) need to be considered. In our previous study, we showed that EDTA could enhance the stromal cell-derived factor 1 alpha-induced migration of DPCs. The purpose of this study wa...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.12.003
更新日期:2020-12-20 00:00:00
abstract::Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.037
更新日期:2006-03-01 00:00:00
abstract:INTRODUCTION:The lysyl oxidase (LOX) family is an emerging family of amine oxidases responsible for the formation of collagen fibrils in the extracellular matrix. To date, 5 LOX family genes have been identified in humans, encoding LOX and LOX-like proteins (LOXL, LOXL2, LOXL3, and LOXL4). The goal of this study was to...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.03.014
更新日期:2012-06-01 00:00:00
abstract::Adsorption of some paraformaldehyde was noted in a previous study evaluating its sterilizing effect on gutta-percha (GP). This study examined histologically the effect of this adsorption when formaldehyde-exposed GP was implanted into the subcutaneous connective tissue of rats. GP implants were prepared in cylinder sh...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81372-5
更新日期:1992-02-01 00:00:00
abstract::Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.03.023
更新日期:2006-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this in vitro study was to compare the cytotoxicity of white mineral trioxide aggregate cement (AMTA, MTA-Angelus), Brasseler Endosequence Root Repair Putty (ERRM), Dycal, and Ultra-blend Plus (UBP) by using human dermal fibroblasts and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.11.012
更新日期:2012-03-01 00:00:00
abstract:INTRODUCTION:Resin-based dental materials contain various diluent monomers that can interfere with vascular function by causing vasodilation. In this study, we evaluated the vasoactive potential of hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) and the possible mechanism of their vascul...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.11.038
更新日期:2011-03-01 00:00:00
abstract:INTRODUCTION:The ability to resolve pulpal inflammation to achieve predictable regeneration of the dentin-pulp complex has remained elusive and presents a challenge for clinicians and researchers. Although the dentin-pulp complex can react naturally to injury by forming a bridge of reparative dentin that protects the p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.06.039
更新日期:2020-09-01 00:00:00
abstract:INTRODUCTION:Clinicians commonly mix mineral trioxide aggregate (MTA) to a desired consistency rather than use the recommended amounts of powder and water. The purpose of this in vitro study was to evaluate how varying the water-to-powder (WP) ratio affects the setting expansion of white MTA (WMTA) and gray MTA (GMTA)....
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2010.03.010
更新日期:2010-08-01 00:00:00
abstract::Twenty-one teeth with clinically asymptomatic periapical pathosis (class 3) were extracted and the isolation, identification, and localization of bacteria in the root apex were examined. Mixtures involving several bacteria were isolated from more than 60% of the cases. Scanning electron microscopy revealed bacterial m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(07)80216-0
更新日期:1990-11-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to evaluate the pH and calcium ion release of calcium hydroxide pastes associated with different substances. METHODS:Forty acrylic teeth with simulated root canals were divided into 4 groups according to the substance associated to the calcium hydroxide paste: chlorhexidine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.05.009
更新日期:2009-09-01 00:00:00
abstract::This study tested the hypothesis that the seal of EndoREZ may be improved with an adhesive-modified technique. Instrumented single-rooted teeth were filled with: (a) Warm vertical compaction with AH Plus (control); (b). EndoREZ with master cones and passive application of accessory cones; (c) Application of Clearfil L...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2006.01.006
更新日期:2006-08-01 00:00:00
abstract::Intracoronal bleaching of pulpless teeth with 30% hydrogen peroxide may result in external cervical root resorption. One of several suggested etiological factors associated with this complication is the pH of the bleaching materials. Because the available data on the pH values of bleaching materials were conflicting, ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81988-6
更新日期:1991-08-01 00:00:00
abstract::Coronal leakage has been recognized as a cause of pulpal and periradicular disease. Although cleaning and shaping of the root canal system is considered paramount, obturation of the system is an important step in endodontic success. The purpose of this study was to evaluate coronal bacterial leakage in teeth obturated...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200204000-00002
更新日期:2002-04-01 00:00:00
abstract::The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80565-0
更新日期:1995-04-01 00:00:00
abstract::A retrograde root-end cover with a special resin composite (Retroplast) combined with the dentin bonding agent Gluma (Bayer AG) has been used since 1984 by the authors. Its content of silver, added to promote radiopacity, has been found to lower the working time and storage stability of the composite and might cause d...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章
doi:10.1016/S0099-2399(96)80082-3
更新日期:1996-09-01 00:00:00
abstract:INTRODUCTION:The objectives of this review were to assess the methodological quality of published meta-analyses related to endodontics using the assessment of multiple systematic reviews (AMSTAR) tool and to provide a follow-up to previously published reviews. METHODS:Three electronic databases were searched for eligi...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2017.07.019
更新日期:2018-01-01 00:00:00