Abstract:
INTRODUCTION:A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 μg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capacity of isolated human dental pulp cells (DPCs) and apical papilla cells (APCs) after a 7-day treatment with selected doses of 3Mix. METHODS:Primary human DPCs/APCs from the third passage were divided into control and experimental groups. In the control group, cells were cultured in regular complete media. In the experimental group, cells were cultured in complete media containing 0.39 μg/mL or 1 mg/mL of 3Mix for 7 days. After the treatment period, the media were changed, and the cells were further tested for proliferation and differentiation potential. For cell proliferation, a colorimetric qualification of 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide was used on days 1, 3, 5, and 7. For differentiation potential, a dentinogenic differentiation medium was added into treated cells and cultured for 7, 14, and 21 days. Results were analyzed using quantitative alizarin red S staining and real-time reverse-transcription polymerase chain reaction. RESULTS:After 7 days of treatment, 100% cell death was discovered in the 1-mg/mL 3Mix group. The proliferative capacity of 0.39 μg/mL 3Mix-treated DPCs and APCs was significantly lower than that of untreated cells at all time points (P < .05). Mineralized nodule formation was found both in the 3Mix-treated and control groups, but it was significantly less in the 3Mix-treated groups at 7, 14, and 21 days (P < .01). Quantitative reverse-transcription polymerase chain reaction showed no statistically significant difference (95% confidence interval) in bone sialoprotein, alkaline phosphatase, and dentin matrix protein 1 gene expression in either 3Mix-treated DPCs or APCs compared with control groups. CONCLUSIONS:One milligram per milliliter of 3Mix had strong toxicity to DPCs/APCs when applied for 7 days, whereas 0.39 μg/mL 3Mix showed no toxicity but still affected cell proliferation and mineralization potential. However, no differences in dentinogenic gene expressions were observed between the 3Mix-treated and untreated groups.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Phumpatrakom P,Srisuwan Tdoi
10.1016/j.joen.2013.09.027subject
Has Abstractpub_date
2014-03-01 00:00:00pages
399-405issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00853-4journal_volume
40pub_type
杂志文章abstract::Mineral Trioxide Aggregate (MTA) has been shown in a number of experiments to be a potential root-end filling material. The purpose of this study was to examine the periradicular tissue response of monkeys to MTA and amalgam as root-end fillings. The pulps were removed from all the maxillary incisors of three monkeys....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80051-9
更新日期:1997-04-01 00:00:00
abstract:INTRODUCTION:Phentolamine mesylate has been reported to be an effective local anesthetic reversal agent for soft tissue but has not been studied regarding reversal of pulpal anesthesia. The authors conducted a prospective randomized, single-blind study comparing the reversal of pulpal and soft tissue anesthesia when ph...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.12.024
更新日期:2013-04-01 00:00:00
abstract::Understanding the chemical and physical characteristics of set mineral trioxide aggregate (MTA) surface can provide insight into its bioactivity. The purpose of this study was to describe the surface chemistry and morphology of gray and white MTA set in the presence of fetal bovine serum (FBS). Eight MTA blocks were p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.09.013
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:The objectives of this review were to assess the methodological quality of published meta-analyses related to endodontics using the assessment of multiple systematic reviews (AMSTAR) tool and to provide a follow-up to previously published reviews. METHODS:Three electronic databases were searched for eligi...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2017.07.019
更新日期:2018-01-01 00:00:00
abstract:INTRODUCTION:Buffered local anesthetics have not been studied for incision and drainage procedures in dentistry. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and pain of an incision and drainage procedure by using a buffered versus a nonbuffered 2% lidocaine wi...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2013.07.008
更新日期:2013-11-01 00:00:00
abstract::A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81411-1
更新日期:1992-04-01 00:00:00
abstract::Many root canal instruments with several different designs have been introduced during recent years. This article evaluates the machining properties of four such instruments: the Flex-R, K-Flex, Trio-Cut, and S file. There are large variations in the machining properties of these instruments. The results are comparabl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(07)80200-7
更新日期:1990-12-01 00:00:00
abstract:INTRODUCTION:The aim of the present study was to assess the bond strength of root fillings in oval-shaped canals prepared with the self-adjusting file (SAF) system. METHODS:A careful specimen selection resulted in 2 equal groups, each consisting of 12 extracted mandibular canines with oval canals that had vital pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.10.026
更新日期:2013-02-01 00:00:00
abstract::With all the potential for endodontic success, the fact remains clinicians are confronted with posttreatment endodontic disease. When the elected treatment plan is endodontic nonsurgical retreatment, then the goal is to access the pulp chamber and remove materials from the root canal space and if present, address defi...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1097/01.don.0000145033.15701.2d
更新日期:2004-12-01 00:00:00
abstract:INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.035
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.006
更新日期:2019-01-01 00:00:00
abstract::The mechanisms of odontogenic pain are complex and incompletely understood. Cases of irreversible pulpitis are thought to represent a localized inflammatory response to bacterial challenge in dental pulp tissue. The presenting symptoms are classically defined by exaggerated painful episodes to thermal stimuli that may...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.01.023
更新日期:2007-05-01 00:00:00
abstract::Gutta-percha (GP) has been the most widely used root canal filling material because of its well-known low toxicity. The inertness of GP, however, was challenged recently. The purpose of this study was to evaluate the toxicity of marketed endodontic GP using the radiochromium release test. Fourteen commercially availab...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)81885-6
更新日期:1990-09-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the shaping ability of 4 different nickel-titanium systems, considering their variation in alloy treatment, kinematics, and design, used to prepare canals with severe curvature using micro-computed tomographic analysis. METHODS:Thirty-two mesial roots of mandibular mol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.02.018
更新日期:2019-06-01 00:00:00
abstract::The purpose of this study was to determine the incidence of endodontic services provided to participants in a longitudinal cohort study. The "Florida Dental Care Study" was a prospective cohort study using a representative baseline sample of 873 dentate adults. An in-person interview and clinical dental exam were cond...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200312000-00005
更新日期:2003-12-01 00:00:00
abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.024
更新日期:2017-09-01 00:00:00
abstract::Unlike most cases of combined orthodontic and endodontic therapy that have been reported, in this case endodontic treatment with calcium hydroxide-induced calcification was utilized prior to orthodontic treatment. A 14-yr-old female required orthodontic therapy due to anterior crowding. Preoperative periapical radiogr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80290-6
更新日期:1994-05-01 00:00:00
abstract::The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200406000-00010
更新日期:2004-06-01 00:00:00
abstract:INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.007
更新日期:2013-12-01 00:00:00
abstract::The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80443-7
更新日期:1995-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effects of endodontic irrigants on the push-out strength and hydration behavior of accelerated mineral trioxide aggregate (MTA) in its early setting phase. METHODS:In an in vitro perforation model, MTA with or without 10% CaCl(2) was condensed and allowed to i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.08.039
更新日期:2010-12-01 00:00:00
abstract::The purpose of this study was to compare ultrasonic and high-speed-bur root-end preparations. Seventy-six roots from 29 bilaterally matched pairs of human teeth in cadavers were used in this study. In group 1 ultrasonic preparations were made in 38 roots and filled with amalgam. In group 2 high-speed bur preparations ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(97)80301-9
更新日期:1997-07-01 00:00:00
abstract:INTRODUCTION:Warm vertically compacted gutta-percha obturation techniques use root canal sealers that are heated during the obturation. This study aims at investigating the suitability of selected sealers with warm gutta-percha obturation techniques. METHODS:The composition of an experimental sealer (Septodont; Saint ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.06.007
更新日期:2015-01-01 00:00:00
abstract::Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200203000-00003
更新日期:2002-03-01 00:00:00
abstract::Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.037
更新日期:2006-03-01 00:00:00
abstract::Because of its close proximity to teeth and periodontium, gingival squamous cell carcinoma (SCC) can sometimes mimic tooth-related benign inflammatory conditions, resulting in misdiagnosis. In this study we report a case of gingival SCC that mimicked a dentoalveolar abscess of endodontic origin in its early presentati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.08.005
更新日期:2007-02-01 00:00:00
abstract:INTRODUCTION:This review aimed to compare the kinematic effect of nickel-titanium instruments with reciprocating and continuous rotation motion for cyclic fatigue resistance, shaping ability, apical debris extrusion, and dentinal defects or cracks. METHOD:Articles were selected for inclusion in this review if they ful...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2016.04.002
更新日期:2016-07-01 00:00:00
abstract::Historically, pulp-necrotizing agents were commonly used in endodontic treatments. They act quickly and devitalize the pulp within a few days. However, they are cytotoxic to gingiva and bone. If such an agent diffuses out of the cavity, it can readily cause widespread necrosis of gingiva and bone, which can lead to os...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000125881.18872.ef
更新日期:2004-10-01 00:00:00
abstract:INTRODUCTION:The purposes of this retrospective study were to evaluate the clinical and radiographic outcomes of mineral trioxide aggregate apexification and revascularization in nonvital immature permanent teeth and to analyze factors influencing treatment outcome. METHODS:Forty-six cases (29 cases of apexification a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.030
更新日期:2017-02-01 00:00:00
abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(96)80285-8
更新日期:1996-03-01 00:00:00