Abstract:
:Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-alpha (TNF-alpha), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1alpha or TNF-alpha and PGE2, either alone or in combination on IL-6 and cyclooxygenase (COX)-2 messenger RNA (mRNA) production in cultured human dental pulp (HDP) fibroblasts. Exposure of HDP fibroblasts to IL-1alpha or TNF-alpha resulted in elevated levels of IL-6 (approximately 3.4 to approximately 10.4-fold) and COX-2 (approximately 5 to approximately 6.2-fold) mRNA. Simultaneous addition of IL-1alpha and PGE2 or TNF-alpha and PGE2 to the cultures significantly reduced the cytokine-induced IL-6 mRNA synthesis ranging from 45% to 65%. However, indomethacin enhanced the cytokine-stimulated IL-6 mRNA synthesis by approximately 1.7 to approximately 3.4-fold. This action could be reversed by exogenous PGE2. In contrast, PGE2 or indomethacin failed to modify the stimulatory effect of IL-1alpha or TNF-alpha on COX-2 gene expression. Because excessive levels of IL-6 and prostaglandins have been connected with the pathogenesis of several inflammatory diseases, our results suggest the involvement of HDP fibroblasts in the development of pulpitis via producing IL-6 and COX-2. Furthermore, expression of IL-6 and COX-2 genes in this cell seems to be differentially regulated by cytokines through prostaglandin-dependent and -independent pathways.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Lin SK,Kuo MY,Wang JS,Lee JJ,Wang CC,Huang S,Shun CT,Hong CYdoi
10.1097/00004770-200203000-00013keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
197-201issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(05)60612-7journal_volume
28pub_type
杂志文章abstract::The choice of one-visit versus two-visit root canal therapy for necrotic teeth with apical periodontitis is a source of current debate. The primary objective of this randomized controlled clinical trial was to compare radiographic evidence of periapical healing after root canal therapy completed in one visit or two vi...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.12.015
更新日期:2008-03-01 00:00:00
abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200306000-00011
更新日期:2003-06-01 00:00:00
abstract:INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.026
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the fracture strength of roots instrumented with the Self-Adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) and the ProTaper system (Dentsply Maillefer, Ballaigues, Switzerland) and filled with the cold lateral compaction technique. METHODS:In total, 50 mandibul...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.030
更新日期:2014-04-01 00:00:00
abstract::This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80158-X
更新日期:1989-12-01 00:00:00
abstract::Porphyromonas endodontalis (formerly Bacteroides endodontalis) is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. The presence of P. endodontalis in infected dental root can...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80843-5
更新日期:1992-09-01 00:00:00
abstract:INTRODUCTION:Various factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.011
更新日期:2019-06-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess temperature changes in conventional gutta-percha, thermoplastic gutta-percha, and Resilon cones by using thermomechanical compaction technique. METHODS:The root canals of extracted human teeth were placed in resin blocks and sectioned. After root canal preparation, cavi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.03.009
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:Pure gutta-percha (trans-1, 4-polyisoprene [TPI]) has been used extensively as a main component of gutta-percha for root canal filling. TPI has the interesting shape memory property by cross-linking, and this polymer was commercialized under the product name of SMP-2 (Kuraray Corp, Kashima, Japan). Therefo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.05.003
更新日期:2014-10-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.07.023
更新日期:2018-11-01 00:00:00
abstract::The purpose of this study was to compare the flow of gutta-percha and Resilon (RealSeal; SybronEndo, Orange, CA) into lateral grooves and depressions in the apical 7 mm of a root canal by using warm vertical compaction. A split-tooth model was constructed with lateral grooves and depressions prepared at 1, 3, 5, and 7...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.02.017
更新日期:2007-06-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to investigate the association between chronic apical periodontitis (CAP) and low-birth-weight preterm births (LBWPB). METHODS:Sixty-three women in postpartum period were included in this case-control study. The case group consisted of mothers of LBWPB infants (n = 33), and...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.11.018
更新日期:2015-03-01 00:00:00
abstract::The aim of this in vitro study was to investigate the effect of the smear layer on apical microleakage in teeth obturated with mineral trioxide aggregate (MTA). Fifty single-rooted central maxillary teeth were used in this study. All teeth were instrumented to size 60 by using the step-back technique. The selected tee...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2008.08.022
更新日期:2008-12-01 00:00:00
abstract:INTRODUCTION:The objective of this ex vivo study was to compare the efficacy of passive ultrasonic irrigation (PUI) versus a new activation system using reciprocating motion (EasyClean [EC]; Easy Equipamentos Odontológicos, Belo Horizonte, Brazil) to remove debris from the root canal walls at 6 predetermined apical lev...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.01.016
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of low-surface tension EDTA solutions on the push-out bond strength of resin-based sealer to young and old root canal dentin. METHODS:Root canals from 64 (n = 32 age <30 years old and n = 32 >60 years old) extracted, decoronized, single-rooted human tee...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.007
更新日期:2018-03-01 00:00:00
abstract::This study evaluated the sealing ability of fast and regular set Super-EBA with different powder-to-liquid ratios as a root-end filling material. Fifty extracted maxillary central incisors were uniformly cleaned and shaped using a crown-down technique with Gates-Glidden drills and Profile series 29.04 taper rotary ins...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80378-1
更新日期:1999-08-01 00:00:00
abstract:INTRODUCTION:The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-α], interleukin [IL]-1β, and IL-6) in human apical periodont...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.10.015
更新日期:2019-02-01 00:00:00
abstract::Occurrence of apical transportation (AT) may complicate the root filling procedure and result in a compromised seal. In part I of this study, human mandibular premolars with single, curved (21 to 39 degrees), or straight canals were prepared by Lightspeed or a step-back hand filing technique. An AT index was determine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200004000-00003
更新日期:2000-04-01 00:00:00
abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80460-7
更新日期:1993-06-01 00:00:00
abstract::An in vitro study was conducted to determine the susceptibility of the yeast Candida albicans to various intracanal irrigants and medications. The minimum inhibitory concentration (MIC) of sodium hypochlorite, hydrogen peroxide, chlorhexidine digluconate, and aqueous calcium hydroxide that is required to kill a standa...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200202000-00004
更新日期:2002-02-01 00:00:00
abstract:INTRODUCTION:This study aimed to evaluate the frequency of dentinal microcracks observed after root canal preparation with 2 reciprocating and a conventional full-sequence rotary system using micro-computed tomographic analysis. METHODS:Thirty mesial roots of mandibular molars presenting a type II Vertucci canal confi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.019
更新日期:2014-09-01 00:00:00
abstract::In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80264-0
更新日期:1996-06-01 00:00:00
abstract:INTRODUCTION:Calcium hydroxide (CH) and nano-calcium hydroxide (NCH) as intracanal medicaments may affect the physical and chemical properties of dentin. The aim of this investigation was to evaluate the effects of CH and NCH on the microhardness and superficial chemical structure of radicular dentin. METHODS:In this ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.06.002
更新日期:2019-09-01 00:00:00
abstract::This study sought to evaluate the influence of apical enlargement on the sealing ability of vertical compaction. Sixty anterior teeth were used. Half of these teeth were prepared to a size 25 file and the other half to a size 40 file. Then vertical compaction was performed. Apical microleakage was determined using pre...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80091-9
更新日期:1994-07-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.034
更新日期:2017-03-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the effect of a range of alkaline pH values on the push-out strength of white mineral trioxide aggregate (WMTA). METHODS:The standardized lumens of root slices prepared from extracted single-rooted human teeth were filled with white ProRoot MTA. The specimens were the...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2010.08.022
更新日期:2010-11-01 00:00:00
abstract::The purpose of this study was to evaluate the number of canals and isthmuses obturated after Cr, Er: YSGG laser treatment. Fifty-two canals were instrumented to a size 40 Profile 0.06 file combined with RC-Prep and sodium hypochlorite, or sterile water. The teeth were divided into 3 groups: (I) canals were flooded wit...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.05.012
更新日期:2007-09-01 00:00:00
abstract::This in vitro study evaluated the survival rate and fracture resistance of maxillary central incisors restored with different post and core systems. The post and core systems investigated were a prefabricated high precious metal post with cast core (group A), zirconia post with a prefabricated bonded ceramic core (gro...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200210000-00008
更新日期:2002-10-01 00:00:00
abstract::The sealing properties of three temporary restorative materials, Cavit, IRM, and a polycarboxylate-based cement, Ultratemp Firm, were investigated in vitro. Standardized access cavities were prepared in 45, intact, extracted, human molars. The teeth were randomly assigned to three groups and the access openings filled...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.don.0000121610.63000.f2
更新日期:2004-08-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to analyze the features of lesions obtained from biopsies at the periapical area of teeth with a radiographic or clinical initial diagnosis of apical periodontitis. METHODS:A retrospective study was conducted on biopsies obtained from 1953-2018 at 3 Brazilian oral and maxillo...
journal_title:Journal of endodontics
pub_type: 杂志文章,多中心研究
doi:10.1016/j.joen.2020.01.003
更新日期:2020-04-01 00:00:00