Differential regulation of interleukin-6 and inducible cyclooxygenase gene expression by cytokines through prostaglandin-dependent and -independent mechanisms in human dental pulp fibroblasts.


:Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-alpha (TNF-alpha), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1alpha or TNF-alpha and PGE2, either alone or in combination on IL-6 and cyclooxygenase (COX)-2 messenger RNA (mRNA) production in cultured human dental pulp (HDP) fibroblasts. Exposure of HDP fibroblasts to IL-1alpha or TNF-alpha resulted in elevated levels of IL-6 (approximately 3.4 to approximately 10.4-fold) and COX-2 (approximately 5 to approximately 6.2-fold) mRNA. Simultaneous addition of IL-1alpha and PGE2 or TNF-alpha and PGE2 to the cultures significantly reduced the cytokine-induced IL-6 mRNA synthesis ranging from 45% to 65%. However, indomethacin enhanced the cytokine-stimulated IL-6 mRNA synthesis by approximately 1.7 to approximately 3.4-fold. This action could be reversed by exogenous PGE2. In contrast, PGE2 or indomethacin failed to modify the stimulatory effect of IL-1alpha or TNF-alpha on COX-2 gene expression. Because excessive levels of IL-6 and prostaglandins have been connected with the pathogenesis of several inflammatory diseases, our results suggest the involvement of HDP fibroblasts in the development of pulpitis via producing IL-6 and COX-2. Furthermore, expression of IL-6 and COX-2 genes in this cell seems to be differentially regulated by cytokines through prostaglandin-dependent and -independent pathways.


J Endod


Journal of endodontics


Lin SK,Kuo MY,Wang JS,Lee JJ,Wang CC,Huang S,Shun CT,Hong CY





Has Abstract


2002-03-01 00:00:00














  • Initial in vitro biological response to contemporary endodontic sealers.

    abstract::The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 x 1 mm). For cytotoxicity testing (MTT method), the spe...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Bouillaguet S,Wataha JC,Tay FR,Brackett MG,Lockwood PE

    更新日期:2006-10-01 00:00:00

  • Temperature triggered shape memory effect of transpolyisoprene-based polymer.

    abstract:INTRODUCTION:Pure gutta-percha (trans-1, 4-polyisoprene [TPI]) has been used extensively as a main component of gutta-percha for root canal filling. TPI has the interesting shape memory property by cross-linking, and this polymer was commercialized under the product name of SMP-2 (Kuraray Corp, Kashima, Japan). Therefo...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Tsukada G,Tokuda M,Torii M

    更新日期:2014-10-01 00:00:00

  • Long-term evaluation of the influence of smear layer on the apical sealing ability of MTA.

    abstract::The aim of this in vitro study was to investigate the effect of the smear layer on apical microleakage in teeth obturated with mineral trioxide aggregate (MTA). Fifty single-rooted central maxillary teeth were used in this study. All teeth were instrumented to size 60 by using the step-back technique. The selected tee...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验


    authors: Yildirim T,Oruçoğlu H,Cobankara FK

    更新日期:2008-12-01 00:00:00

  • Is a volume of 3.6 mL better than 1.8 mL for inferior alveolar nerve blocks in patients with symptomatic irreversible pulpitis?

    abstract:INTRODUCTION:The purpose of this retrospective study was to determine the success of the inferior alveolar nerve (IAN) block using either 3.6 mL or 1.8 mL 2% lidocaine with 1:100,000 epinephrine in patients presenting with symptomatic irreversible pulpitis. METHODS:As part of 7 previously published studies, 319 emerge...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Fowler S,Reader A

    更新日期:2013-08-01 00:00:00

  • Inflammation triggers constitutive activity and agonist-induced negative responses at M(3) muscarinic receptor in dental pulp.

    abstract::The purpose of this study was to investigate whether the inflammation of rat dental pulp induces the muscarinic acetylcholine receptor (mAChR) constitutive receptor activity. Pulpitis was induced with bacterial lipolysaccharide in rat incisors dental pulp. Saturation assay with [(3)H]-quinuclidinyl benzilate ([(3)H] Q...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Sterin-Borda L,Orman B,De Couto Pita A,Borda E

    更新日期:2011-02-01 00:00:00

  • Inflammatory Myofibroblastic Tumor Mimicking Apical Periodontitis.

    abstract::Inflammatory myofibroblastic tumors (IMTs) are rare. IMTs of the head and neck occur in all age groups, from neonates to old age, with the highest incidence occurring in childhood and early adulthood. An IMT has been defined as a histologically distinctive lesion of uncertain behavior. This article describes an unusua...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Adachi M,Kiho K,Sekine G,Ohta T,Matsubara M,Yoshida T,Katsumata A,Tanuma J,Sumitomo S

    更新日期:2015-12-01 00:00:00

  • Effects of raw material and rotational speed on the cyclic fatigue of ProFile Vortex rotary instruments.

    abstract:INTRODUCTION:This in vitro study compared the cyclic fatigue resistance of ProFile Vortex rotary instruments (DENTSPLY Tulsa Dental, Tulsa, OK) made of two different raw materials: M-Wire and regular superelastic wire (SE-wire) at two different rotational speeds. Two rotary-based Profile Vortex instruments with taper 0...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Gao Y,Shotton V,Wilkinson K,Phillips G,Johnson WB

    更新日期:2010-07-01 00:00:00

  • Leakage associated with single or multiple increment backfill with the Obtura II gutta-percha system.

    abstract::The purpose of this in vitro study was to compare dye leakage between canals backfilled in a single increment and canals backfilled in multiple increments using the Obtura II system with two different sealers. Sixty extracted single canal teeth were decoronated, cleaned, and shaped. After master cone fit and placement...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Johnson BT,Bond MS

    更新日期:1999-09-01 00:00:00

  • Patient-specific Finite Element Analysis of Fiber Post and Ferrule Design.

    abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Rodrigues MP,Soares PBF,Valdivia ADCM,Pessoa RS,Veríssimo C,Versluis A,Soares CJ

    更新日期:2017-09-01 00:00:00

  • Effect of Diclofenac Potassium Premedication on Postendodontic Pain in Mandibular Molars with Symptomatic Irreversible Pulpitis: A Randomized Placebo-Controlled Double-Blind Trial.

    abstract:INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验


    authors: Al-Rawhani AH,Gawdat SI,Wanees Amin SA

    更新日期:2020-08-01 00:00:00

  • Evaluation of the apical condensation of gutta-percha by a tapered/calibrated spreader/plugger.

    abstract::This study compared a tapered, calibrated spreader/plugger instrument (M-series) to noncalibrated spreaders and pluggers in achieving apical condensation of gutta-percha in 20 single-rooted human teeth. The distances between the tip of the instruments and the tip of the master gutta-percha were measured. Results showe...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Chohayeb AA

    更新日期:1993-04-01 00:00:00

  • The Outcome of Primary Root Canal Treatment in Postirradiated Patients: A Case Series.

    abstract::Osteoradionecrosis (ORN) of the jaw is considered the most severe long-term adverse effect that can occur in radiation therapy patients. Teeth extractions and dental diseases are the main risk factors for ORN in irradiated patients. The aim of this case series was to evaluate the outcome of primary root canal treatmen...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Castagnola R,Minciacchi I,Rupe C,Marigo L,Grande NM,Contaldo M,Pesce A,Lajolo C

    更新日期:2020-04-01 00:00:00

  • Effect of sodium fluorosilicate on the properties of Portland cement.

    abstract:INTRODUCTION:Mineral trioxide aggregate (MTA) satisfies most of the ideal properties of a surgical root-end filling and perforation repair material. It has been found to be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable and promotes cementogenesis. The major...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Appelbaum KS,Stewart JT,Hartwell GR

    更新日期:2012-07-01 00:00:00

  • The number of bleaching sessions influences pulp tissue damage in rat teeth.

    abstract:INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Cintra LT,Benetti F,da Silva Facundo AC,Ferreira LL,Gomes-Filho JE,Ervolino E,Rahal V,Briso AL

    更新日期:2013-12-01 00:00:00

  • Removal of surgical smear layer.

    abstract:INTRODUCTION:During apicoectomy and retrograde cavity preparation, a smear layer, which contains microorganisms and necrotic pulpal tissues, is formed on the dentinal surfaces cut by the instruments. Bacteria can survive and proliferate inside or below the smear layer. The purpose of this study was to evaluate in vitro...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验


    authors: Fabiani C,Franco V,Covello F,Brambilla E,Gagliani MM

    更新日期:2011-06-01 00:00:00

  • Removal of Root Canal Fillings in Curved Canals Using Either Reciprocating Single- or Rotary Multi-instrument Systems and a Supplementary Step with the XP-Endo Finisher.

    abstract:INTRODUCTION:This study compared the efficacy of a reciprocating single-instrument system and a rotary multi-instrument system followed by a supplementary approach with a finishing instrument in removing the filling material from curved canals during retreatment. METHODS:Forty mesial canals from extracted mandibular m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Alves FR,Marceliano-Alves MF,Sousa JC,Silveira SB,Provenzano JC,Siqueira JF Jr

    更新日期:2016-07-01 00:00:00

  • The effect of antibiotics and endodontic antimicrobials on the polymerase chain reaction.

    abstract::The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Fouad AF,Barry J

    更新日期:2005-07-01 00:00:00

  • Canal blockage and debris extrusion with eight preparation techniques.

    abstract::The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: al-Omari MA,Dummer PM

    更新日期:1995-03-01 00:00:00

  • The concentration of prostaglandin E2 in human periradicular lesions.

    abstract::Prostaglandins (PG) have been implicated in the genesis of periapical lesions. Periapical specimens from patients with clinical signs and symptoms of chronic and acute apical periodontitis were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars were frozen and used as...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: McNicholas S,Torabinejad M,Blankenship J,Bakland L

    更新日期:1991-03-01 00:00:00

  • Anatomical relationship of the mandibular canal to its surrounding structures in mature mandibles.

    abstract::A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Denio D,Torabinejad M,Bakland LK

    更新日期:1992-04-01 00:00:00

  • Role of the extracellular signal-regulated kinase 1/2 pathway in driving tricalcium silicate-induced proliferation and biomineralization of human dental pulp cells in vitro.

    abstract:INTRODUCTION:The aim of this study was to investigate the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in regulating tricalcium silicate (C3S)-driven proliferation and biomineralization of human dental pulp cells (hDPCs) in vitro. METHODS:Human DPCs were cultured in C3S-containing medium and ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Du R,Wu T,Liu W,Li L,Jiang L,Peng W,Chang J,Zhu Y

    更新日期:2013-08-01 00:00:00

  • Torsional Performance of ProTaper Gold Rotary Instruments during Shaping of Small Root Canals after 2 Different Glide Path Preparations.

    abstract:INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Arias A,de Vasconcelos RA,Hernández A,Peters OA

    更新日期:2017-03-01 00:00:00

  • Extraoral sinus tract misdiagnosed as an endodontic lesion.

    abstract::The extraoral sinus tract may occur as a result of an inflammatory process associated with a necrotic pulp. However, several non-odontogenic disorders may also produce an extraoral sinus tract. Thus, the differential diagnosis of this clinical finding is of paramount importance in providing appropriate clinical care b...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Cohenca N,Karni S,Rotstein I

    更新日期:2003-12-01 00:00:00

  • Idiopathic Bone Cavity: Clinicopathologic Features from a Case Series with Emphasis on Differential Diagnosis of Chronic Apical Periodontitis.

    abstract:INTRODUCTION:Idiopathic bone cavity (IBC) is an uncommon bone lesion that usually affects youngsters as an unilocular radiolucency with predilection for the posterior mandible. Because the lesion is frequently located in proximity to the adjacent teeth, chronic apical periodontitis is commonly included as a differentia...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Roma LG,Lopes CB,Netto JNS,Miranda ÁMMA,Pires FR

    更新日期:2021-02-01 00:00:00

  • An in vitro evaluation of the sealing ability of a new root-canal-obturation system.

    abstract::In this study the sealing ability of a new urethane methacrylate resin-based sealer, EndoRez, was evaluated using a fluid-filtration model. Sixty-four single-rooted lower bicuspids were decoronated, instrumented, and divided into 3 groups of 20 each with 4 teeth used as controls. In group A, the roots were obturated w...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Kardon BP,Kuttler S,Hardigan P,Dorn SO

    更新日期:2003-10-01 00:00:00

  • Denaturation temperatures of dentin matrices. I. Effect of demineralization and dehydration.

    abstract::The denaturation temperature (T(d)) of dentin collagen in mineralized versus demineralized teeth was examined as a function of dentin age and the extent of dehydration. Using differential scanning calorimetry, T(d) of mineralized dentin was shown to be between 160 degrees C to 186 degrees C, depending on whether it wa...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Armstrong SR,Jessop JL,Winn E,Tay FR,Pashley DH

    更新日期:2006-07-01 00:00:00

  • Orthodontic procedures after trauma.

    abstract::This review considers oral trauma and its relationship to orthodontics with respect to prevention and primary, secondary, and tertiary care. The level of evidence is not high in regard to this topic, but recommendable approaches to trauma at each stage are discussed on the basis of available literature and published g...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,评审


    authors: Fields HW,Christensen JR

    更新日期:2013-03-01 00:00:00

  • Bleaching effect of sodium percarbonate on discolored pulpless teeth in vitro.

    abstract::The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolars were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleach...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Kaneko J,Inoue S,Kawakami S,Sano H

    更新日期:2000-01-01 00:00:00

  • Ultramorphological and histochemical changes after ER,CR:YSGG laser irradiation and two different irrigation regimes.

    abstract::The purpose of this study was to evaluate the ultramorphological and chemical changes in Er,Cr:YSGG laser-treated radicular dentin in comparison with two different irrigation regimes. Extracted human premolars were assigned into three experimental groups. Group 1: After each file size, root canals were irrigated with ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章


    authors: Altundasar E,Ozçelik B,Cehreli ZC,Matsumoto K

    更新日期:2006-05-01 00:00:00

  • Effect of gutta-percha solvents on mineral contents of human root dentin using ICP-AES technique.

    abstract::The purpose of this in vitro study was to evaluate the mineral contents of root-canal dentin before and after treatment with two commonly used gutta-percha solvents: chloroform and halothane. Twenty extracted human premolars, whose crowns and apical thirds had been removed, were used. Pulp tissues were removed and the...

    journal_title:Journal of endodontics

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Erdemir A,Eldeniz AU,Belli S

    更新日期:2004-01-01 00:00:00