Abstract:
INTRODUCTION:Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold may attract resident cells to form pulplike tissue. In this study, an ectopic animal model for in situ dental pulp tissue engineering was developed to evaluate whether pulplike tissue formation in empty root canals after the attraction of stem cells was possible and whether this could be enhanced by dentin-derived growth factors. METHODS:Three types of fibrin (custom-made fibrin, fibrin sealant, and plasma rich in growth factors [PRGF]) as well as a self-assembling peptide were evaluated in vivo in a modified tooth root model using human teeth. Root canal dentin was conditioned with EDTA, tooth roots were filled with growth factor-laden scaffolds, and dental pulp stem cells in collagen were placed at the root tip. Constructs were implanted into immunocompromised mice for 4 weeks and subsequently analyzed histologically. Differential interference contrast and second harmonic generation imaging were performed for selected sections. RESULTS:For custom-made fibrin and fibrin sealant with dentin matrix proteins, migration into the roots and the formation of a pulplike tissue were observed, whereas the peptide-based scaffold appeared less suitable. PRGF supported tissue formation regardless of the addition of dentin matrix proteins. In the test groups with dentin matrix proteins and EDTA conditioning, differentiated odontoblastlike cells extended cellular processes into the dentinal tubules, which coincided with the deposition of the newly formed collagenous dentin matrix. CONCLUSIONS:This new cell homing model provides evidence that fibrin derivatives make applicable scaffolds and that dentin-derived proteins induce chemotaxis and pulplike tissue formation.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Widbiller M,Driesen RB,Eidt A,Lambrichts I,Hiller KA,Buchalla W,Schmalz G,Galler KMdoi
10.1016/j.joen.2018.02.011subject
Has Abstractpub_date
2018-06-01 00:00:00pages
956-962.e2issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30090-6journal_volume
44pub_type
杂志文章abstract:INTRODUCTION:The purpose of the present study was to evaluate the thickness and the anatomic characteristics of the Schneiderian membrane and cortical bone using limited cone beam computed tomography (CBCT) scannning in patients referred for planning of apical surgery of maxillary molars. METHODS:This controlled study...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.09.023
更新日期:2012-01-01 00:00:00
abstract::Intermediate Restorative Material (IRM) has been advocated as a root-end filling material based primarily on reports of clinical success and various leakage studies. The objectives of this study were to determine the excisional wound healing responses of the periradicular tissues to IRM root-end filling material and t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80201-4
更新日期:1997-01-01 00:00:00
abstract::A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200206000-00001
更新日期:2002-06-01 00:00:00
abstract::The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80443-7
更新日期:1995-03-01 00:00:00
abstract::Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000128749.50151.24
更新日期:2004-12-01 00:00:00
abstract:INTRODUCTION:This finite element analysis study was aimed at evaluating the effects of rotary systems on stresses in photopolymerized resin root models with oval-shaped canals. METHODS:Among the data collected by computed tomographic imaging, a mandibular second premolar tooth with an oval-shaped canal was selected, r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.007
更新日期:2016-08-01 00:00:00
abstract::Stem cell therapy is a potential strategy to regenerate the dentin-pulp complex, enabling the conservation and restoration of functional teeth. We assessed the efficacy and safety of pulp stem cell transplantation as a prelude before the initiation of clinical trials. Granulocyte-colony stimulating factor (G-CSF) indu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.020
更新日期:2014-04-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess 2 cone-beam computed tomographic systems on the detection of artificially induced vertical root fractures (VRFs) and artifact intensity using birooted teeth restored with different intracanal materials. METHODS:The sample consisted of 20 extracted birooted premolars. Ro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.028
更新日期:2020-02-01 00:00:00
abstract::Ultrasonic instruments are a valuable asset for removing intraradicular posts from root canals before nonsurgical endodontic therapy. The purpose of this study was to evaluate the relative efficacy of post removal by ultrasonic and sonic devices. Fifty extracted teeth were instrumented and obturated. The canals were p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80044-0
更新日期:1994-10-01 00:00:00
abstract::K-type files, Sureflex NiTi files, and FlexoFiles were evaluated morphometrically as they come from the manufacturer, and after being used to instrument root canals 1, 3, and 5 times in maxillary premolars. Evaluation was made using a stereomicroscope at x40 magnification and photomicrographs. The abnormalities found ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(98)80046-0
更新日期:1998-07-01 00:00:00
abstract::Various cells and inflammatory mediators are involved in the initial pulpal responses to caries. This review focuses on the cellular, neuronal, and vascular components of pulpal innate responses to caries. Discussion will include dentinal fluid, odontoblasts, neuropeptides, and neurogenic inflammation, which are not c...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2007.01.001
更新日期:2007-06-01 00:00:00
abstract::Sodium hypochlorite (NaOCl) has been recommended for irrigation during root canal preparation. This investigation used scanning electron microscopy to examine instrumented and uninstrumented surfaces in the middle third of root canals following the use of several concentrations of NaOCl (5.25%, 2.5%, 1.0%, and 0.5%). ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81331-2
更新日期:1992-12-01 00:00:00
abstract::Extracanal invasive resorption is not a well-defined phenomenon. This article describes its clinical, radiographic, and histological characteristics. Treatment of affected teeth based on the location of the resorptive defect is also described. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80056-3
更新日期:1998-07-01 00:00:00
abstract::With the recent influx of osseointegrated endosseous implants in dentistry, attention has been drawn away from using endodontic stabilizers as a means of stabilizing and retaining teeth. It is our opinion that endodontic stabilizers should be considered in the treatment planning of seemingly nonretainable teeth. They ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81268-9
更新日期:1992-05-01 00:00:00
abstract:INTRODUCTION:This study evaluated the cyclic fatigue resistance (CFR) of Reciproc (R25 and R40; VDW, Munich, Germany) and Reciproc Blue (R25 and R40, VDW) instruments used in an artificial S-shaped canal. METHODS:A total of 80 files were tested in an S-shaped canal (n = 20 for each file, Reciproc R25 and R40 and Recip...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.009
更新日期:2017-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the accuracy in vivo of 2 electronic apex locators (EALs), the Raypex 5 and the Mini Apex Locator. METHODS:The working length (WL) was determined electronically for 40 human root canals by using a K-file and 1 of the 2 EALs. The files were fixed at the WL, and the ...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2011.06.021
更新日期:2011-10-01 00:00:00
abstract::This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a sta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80521-2
更新日期:1995-10-01 00:00:00
abstract::Sixty-eight standardized human root specimens were infected with Enterococcus faecalis for 3 wk after removal of the smear layer. After 3 wk of infection, the smear layer was reformed and in half of the specimens, the smear layer was again removed. Aqueous Ca(OH)2 paste and silicone oil based Ca(OH)2 paste were used a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200105000-00004
更新日期:2001-05-01 00:00:00
abstract:INTRODUCTION:Endodontic diagnostic tests are often used clinically to assess pulp status as a basis for the diagnosis and determination of whether root canal treatment (RCT) is indicated. Response to cold and pain on percussion are 2 common tests, yet their validity in identifying nonvital pulp in regular dental practi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.03.016
更新日期:2016-06-01 00:00:00
abstract::Adsorption of some paraformaldehyde was noted in a previous study evaluating its sterilizing effect on gutta-percha (GP). This study examined histologically the effect of this adsorption when formaldehyde-exposed GP was implanted into the subcutaneous connective tissue of rats. GP implants were prepared in cylinder sh...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81372-5
更新日期:1992-02-01 00:00:00
abstract:INTRODUCTION:In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.07.003
更新日期:2013-11-01 00:00:00
abstract:INTRODUCTION:This in vitro study compared cone-beam computed tomography (CBCT) exam with different voxel sizes with digital periapical radiography in the detection of vertical root fractures in teeth with and without intracanal metallic posts. METHODS:Eighteen single-rooted human teeth were endodontically treated, pre...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.031
更新日期:2013-12-01 00:00:00
abstract::In the present study, we used Fos expression as an index of nociceptive input to the spinal trigeminal nucleus after exposure of the coronal pulp tissue of maxillary right first molars and examined the effects of pretreatment with an opioid, a nonsteroidal anti-inflammatory drug or a local anesthetic before pulp expos...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000153844.61709.64
更新日期:2005-08-01 00:00:00
abstract::Pulp revascularization is a promising procedure for the treatment of adolescents' immature permanent teeth with necrotic pulp and/or apical periodontitis. However, the ability to successfully perform pulp revascularization in a middle-aged patient remains unclear. A 39-year-old woman was referred for treatment of teet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::In the past, measurement of microleakage has been based on measurement of dye or radioactive isotope penetration. A method has been developed to allow electronic monitoring of microflow in the same specimen over a period of time. This method was used to compare previously reported retrograde materials with an amalgam/...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80072-5
更新日期:1994-11-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to examine the effect of pulp cell injection on host angiogenesis during wound healing. METHODS:Pulp cells were isolated from extracted premolars by the outgrowth method. Fluorescently labeled pulp cells or phosphate-buffered saline were locally injected into a mouse wound healin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.01.011
更新日期:2013-06-01 00:00:00
abstract:INTRODUCTION:Squamous odontogenic tumor-like epithelial islands occurring in the walls of odontogenic cysts are histologically identical to the squamous odontogenic tumor (SOT). Microscopically, the squamous odontogenic tumor-like proliferations (SOTLPs) share certain histologic features with SOT, acanthomatous and des...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.02.010
更新日期:2011-05-01 00:00:00
abstract:INTRODUCTION:Instrumentation of the mesial root canal system of mandibular molars may hinder disinfection by packing hard tissue debris within the isthmuses. The removal of accumulated hard tissue debris (AHTD) by 3 supplemental irrigation systems, 2 ultrasonically activated and 1 multisonic, was assessed with micro-co...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.013
更新日期:2019-07-01 00:00:00
abstract::The cytotoxic effects of a new epoxy resin-based root canal sealer (AH-plus), together with those of two other commonly used endodontic sealers (AH26 and zinc oxide-eugenol), have been studied in vitro on a culture of human gingival fibroblasts. Cytotoxicity was assessed by direct incubation of sealers' extracts with ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200008000-00008
更新日期:2000-08-01 00:00:00
abstract::This study evaluated the ability of ultrasonics and calcium hydroxide to remove pulp tissue debris from the mesial root canals of human mandibular molars. All teeth were instrumented using a standard filing technique and irrigated with an equal volume of 2.6% sodium hypochlorite before the application of the experimen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(89)80076-7
更新日期:1989-08-01 00:00:00