Abstract:
INTRODUCTION:The aim of this study was to examine the effect of pulp cell injection on host angiogenesis during wound healing. METHODS:Pulp cells were isolated from extracted premolars by the outgrowth method. Fluorescently labeled pulp cells or phosphate-buffered saline were locally injected into a mouse wound healing model. Wound healing was evaluated using photographs, histology, and real-time reverse-transcription polymerase chain reaction. Injected cells were traced. Angiogenesis was measured by performing immunohistochemical staining of CD31, a marker of vascular endothelial cells. The level of secreted vascular endothelial growth factor in the pulp cell conditioned medium (CM) was compared with the CM of fibroblasts and keratinocytes. The paracrine effect of pulp CM on angiogenesis was evaluated by tubular network formation using endothelial cells. RESULTS:The local injection of pulp cells enhanced wound closure during the initial stage when compared to the injection of phosphate-buffered saline. The amount of extracellular matrix production and the expression of CD31+ cells were also increased in response to pulp cell injection when compared with the injection of phosphate-buffered saline. The fluorescently labeled pulp cells were engrafted into the hair follicles of the adjacent normal dermis but not into the wound site per se. A significantly higher level of vascular endothelial growth factor was secreted into the CM of pulp cells when compared with dermal fibroblast and keratinocytes. Tubular network formation of endothelial cells and the proliferation of dermal fibroblasts were significantly enhanced by the application of pulp cell CM when compared with control media. CONCLUSIONS:Our results show that local injection of pulp cells is effective in enhancing wound healing during the initial proliferative phase, especially through paracrine mechanisms regulating host angiogenesis and proliferation.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Yang H,Shin S,Ahn J,Choi Y,Kim KH,Chung CJdoi
10.1016/j.joen.2013.01.011subject
Has Abstractpub_date
2013-06-01 00:00:00pages
788-94issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00139-8journal_volume
39pub_type
杂志文章abstract:INTRODUCTION:More information regarding the ability of single files to maintain the original canal position is required before adopting a single-file approach to cleaning and shaping. This study compares apical transportation in curved root canals when instrumenting with a single WaveOne (WO) file (Dentsply Tulsa Denta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.021
更新日期:2014-05-01 00:00:00
abstract::A technique using K files with modified tips has been developed for the penetration of constricted canals. The tip of a #10 K file was sliced diagonally to make it thinner. Because this modified K file has an especially fine tip and an appropriate stiffness, it followed constricted or sharply curved canals well and ha...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80190-2
更新日期:1997-06-01 00:00:00
abstract::The occurrence of Enterococcus faecalis in root canals of previously root filled teeth with apical periodontitis requiring retreatment was studied in Lithuanian patients. Twenty-five asymptomatic teeth were included in the study. Avoiding contamination microbiological samples were taken from the canals before and afte...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200010000-00004
更新日期:2000-10-01 00:00:00
abstract::Pulpitis, external root resorption, and pain may be experienced during orthodontic movement. The use of nonsteroidal anti-inflammatory drugs (NSAIDs) has been suggested to control these changes. The purpose of this study was to observe pulp-dentinal reactions, root resorption, tooth pain, and tooth movement after the ...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.don.0000134212.20525.74
更新日期:2005-01-01 00:00:00
abstract::This study compared the weight and radiographic appearance of Ca(OH)(2) delivered into standardized, minimally and fully prepared canals using (a) an injection system, (b) an endodontic Flex-O file rotated counter-clockwise, (c) a lentulo spiral, and (d) a .04 rotary NiTi file rotated in reverse. Two extracted human s...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.01.009
更新日期:2006-07-01 00:00:00
abstract::Use of resin-based restorative materials recently has become widely accepted for treatment of endodontically treated teeth. However, some solutions routinely used during endodontic treatment procedures may have an effect on bond strengths of adhesive materials to root canal dentin. The purpose of this in vitro study w...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200402000-00013
更新日期:2004-02-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to investigate the influence of apical filling material and the modification made to the apical preparation design in surgical endodontics on the areas of stress concentration in the mesial root of a mandibular molar using finite element analysis. METHODS:The filling material was...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.12.007
更新日期:2020-04-01 00:00:00
abstract:INTRODUCTION:This study evaluated the cyclic fatigue resistance (CFR) of Reciproc (R25 and R40; VDW, Munich, Germany) and Reciproc Blue (R25 and R40, VDW) instruments used in an artificial S-shaped canal. METHODS:A total of 80 files were tested in an S-shaped canal (n = 20 for each file, Reciproc R25 and R40 and Recip...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.009
更新日期:2017-10-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to investigate the expression of matrix metalloproteinases (MMPs) in apical periodontitis and during the periapical healing phase after root canal treatment. METHODS:Apical periodontitis was induced in dog teeth, and root canal treatment was performed in a single visit or b...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.10.030
更新日期:2010-02-01 00:00:00
abstract::This study is a quantitative assessment of the luminous energy transmitted through different translucent fiber posts. After embedding the posts in black resin, the blocks were submitted to sequential cuts in a precision machine, and depths of 16 mm, 12 mm, 8 mm, and 4 mm were assessed for light transmission with a dig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.12.007
更新日期:2008-03-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the sensitivity and specificity of cone-beam computed tomography (CBCT) scans and digital periapical radiographs (PR) in detecting strip and root perforations after root canal treatment in mandibular molars. METHODS:Forty-five curved mesial roots were endodontically pr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.12.003
更新日期:2011-04-01 00:00:00
abstract::The purpose of this study was to evaluate the strengthening effect of an internal resin bonding technique. One hundred central incisors were endodontically treated and divided equally into five groups. Group 1, without any cervical preparation and with access repaired with bonded resin, served as the positive control....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(98)80108-8
更新日期:1998-04-01 00:00:00
abstract:INTRODUCTION:The ability to resolve pulpal inflammation to achieve predictable regeneration of the dentin-pulp complex has remained elusive and presents a challenge for clinicians and researchers. Although the dentin-pulp complex can react naturally to injury by forming a bridge of reparative dentin that protects the p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.06.039
更新日期:2020-09-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the efficacy of passive ultrasonic irrigation (PUI) and the EndoVac (EV) System (Discus Dental, Culver City, CA) in hard tissue debris removal and its influence on the quality of the root canal filling with the aid of micro-computed tomographic scanner. METHODS:Twenty-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.05.001
更新日期:2015-10-01 00:00:00
abstract:INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.03.006
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:Different microbial communities have been associated with acute endodontic infections. The majority of the microorganisms are as yet uncultivable or difficult to grow under current laboratory conditions. Treponema species are strict anaerobic bacteria that are involved in several oral diseases. The aim of ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.08.008
更新日期:2010-11-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the effect of a range of alkaline pH values on the push-out strength of white mineral trioxide aggregate (WMTA). METHODS:The standardized lumens of root slices prepared from extracted single-rooted human teeth were filled with white ProRoot MTA. The specimens were the...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2010.08.022
更新日期:2010-11-01 00:00:00
abstract::The endodontic endosseous implant (EEI) enjoyed wide use in the United States after it was introduced in the 1960's. For various reasons including incorrect case selection, improper use of the materials, and poor preparation for the implant a high number of failures resulted. Following opposition by some members of th...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81496-2
更新日期:1993-10-01 00:00:00
abstract:INTRODUCTION:Pure gutta-percha (trans-1, 4-polyisoprene [TPI]) has been used extensively as a main component of gutta-percha for root canal filling. TPI has the interesting shape memory property by cross-linking, and this polymer was commercialized under the product name of SMP-2 (Kuraray Corp, Kashima, Japan). Therefo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.05.003
更新日期:2014-10-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to evaluate the pH and calcium ion release of calcium hydroxide pastes associated with different substances. METHODS:Forty acrylic teeth with simulated root canals were divided into 4 groups according to the substance associated to the calcium hydroxide paste: chlorhexidine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.05.009
更新日期:2009-09-01 00:00:00
abstract::This randomized, double-blind trial tested the null hypothesis that speed of deposition has no influence on the injection discomfort, efficacy, distribution, and duration of pulp anesthesia after incisive/mental nerve block in adult volunteers. Thirty-eight subjects received incisive/mental nerve blocks of 2.0 mL lido...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.07.016
更新日期:2007-10-01 00:00:00
abstract::Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200203000-00003
更新日期:2002-03-01 00:00:00
abstract:INTRODUCTION:Recently, it was shown that the biomineralization process promoted by the interaction of mineral trioxide aggregate (MTA) with dentin in phosphate-buffered saline (PBS) positively influenced the push-out bond strength of the cement. This study investigated if the use of a PBS intracanal dressing promotes t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.06.014
更新日期:2010-10-01 00:00:00
abstract::The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.009
更新日期:2018-05-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate and compare the antimicrobial activity of calcium hydroxide, triantibiotic mixture (TAM), and an ethanol extract of propolis as intracanal medicaments on Enterococcus faecalis-infected root canals. METHODS:One hundred twenty extracted intact human permanent incisors w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.028
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.015
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:This study aimed at evaluating the bond strength to root dentin of 2 mineral trioxide aggregate (MTA)-based sealers (Endo-CPM sealer and MTA Fillapex) and of 1 epoxy resin-based sealer (AH Plus sealer). METHODS:Forty-five extracted human teeth with single roots were prepared by using the step-back techniq...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.10.018
更新日期:2012-02-01 00:00:00
abstract::The purpose of this study was to evaluate the influence of Hero 642 rotary Ni-Ti instruments driven at 300, 400, or 600 rpm on root canal straightening, loss of working length, and instrument breakage. Sixty mesial root canals from extracted human mandibular molars were divided into 3 groups of 20 each and were instru...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200307000-00005
更新日期:2003-07-01 00:00:00
abstract:INTRODUCTION:The aim was to present a novel surgical technique using virtually preplanned 3-dimensional (3D)-printed templates for guided osteotomies. These were to ensure atraumatic uncovering of a severely impacted donor tooth including guided drilling of the recipient alveolus followed by a secure autotransplantatio...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.07.024
更新日期:2020-07-29 00:00:00
abstract::Mineral Trioxide Aggregate (MTA) has been shown in a number of experiments to be a potential root-end filling material. The purpose of this study was to examine the periradicular tissue response of monkeys to MTA and amalgam as root-end fillings. The pulps were removed from all the maxillary incisors of three monkeys....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80051-9
更新日期:1997-04-01 00:00:00