Abstract：
:Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde apical obturation with MTA; three groups had 1-, 2-, or 3-mm thickness. Sterilized specimens were inoculated with E. faecalis and incubated in sterile medium. DNA extracted from the specimens was amplified by polymerase chain reaction, which yielded a specific segment of E. faecalis 16S rDNA. On day 10 of incubation, no specimens were contaminated. On day 50, almost 17% of specimens were contaminated, with no statistically significant difference between groups (Chi-square = 0.48; df = 2; p = 0.787). Therefore, MTA provides an adequate seal even in cases of orthograde apical obturation of pulpless teeth with open apices.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
de Leimburg ML,Angeretti A,Ceruti P,Lendini M,Pasqualini D,Berutti Edoi
10.1097/01.don.0000128749.50151.24keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
883-6issue
12eissn
0099-2399issn
1878-3554pii
00004770-200412000-00014journal_volume
30pub_type
杂志文章abstract：INTRODUCTION:Endodontic claims are the most frequently filed malpractice claims in dentistry. The aim of this study was to categorize and review errors and complications related to endodontic procedures that resulted in legal actions against the treating practitioner. METHODS:All dental malpractice complaints reported...
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pub_type： 临床试验,杂志文章
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更新日期：1996-09-01 00:00:00
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更新日期：1997-10-01 00:00:00
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