Abstract:
INTRODUCTION:Lipopolysaccharide (LPS) has been shown to regulate the function of odontoblasts. However, the molecular mechanisms of the effect of LPS on odontoblasts are poorly understood. Decorin (DCN), one of the major matrix proteoglycans, is known to affect the mineralization of teeth. In this study, we investigated whether LPS can regulate the expression of DCN in odontoblasts and determined the intracellular signaling pathways triggered by LPS. METHODS:The DCN messenger RNA and protein expression changes in mouse odontoblast-lineage cells (OLCs) were detected by real-time polymerase chain reaction (PCR) analysis and enzyme-linked immunosorbent assay (ELISA). Whether TLR4, myeloid differentiating factor 88 (MyD88), nuclear factor-kappa B (NF-κB), or mitogen-activated protein kinase (MAPK) pathways were involved in the LPS-induced DCN expression was determined by examined real-time PCR, ELISA, and luciferase activity assay. The activation of extracellular signal-regulated kinase (ERK), p38, and JNK in OLCs was measured by Western blot analysis. RESULTS:We found that the mouse OLCs expressed DCN. DCN messenger RNA was rapidly induced by LPS in a time- and dose-dependent manner. Pretreatment with a MyD88 inhibitory peptide, a TLR4 antibody, or a specific inhibitor for NF-κB or I Kappa B alpha (IκBα) significantly inhibited LPS-induced DCN expression. Moreover, the LPS-mediated increase in κB-luciferase activity in OLCs was suppressed by the overexpression of dominant negative mutants of TLR4, MyD88, and IκBα but not by a dominant negative mutant of TLR2. In addition, LPS stimulation activated the ERK, p38, and JNK MAPK pathways. The pretreatment of OLCs with specific inhibitors of the ERK, p38, and JNK MAPK pathways markedly offset the LPS-induced up-regulation of DCN expression. CONCLUSIONS:Our results show that LPS stimulation can up-regulate the gene expression of DCN via the TLR4, MyD88, NF-κB, and MAPK pathways in odontoblast cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
He W,Qu T,Yu Q,Wang Z,Wang H,Zhang J,Smith AJdoi
10.1016/j.joen.2011.12.021subject
Has Abstractpub_date
2012-04-01 00:00:00pages
464-9issue
4eissn
0099-2399issn
1878-3554pii
S0099-2399(11)01454-3journal_volume
38pub_type
杂志文章abstract::Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study,...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200308000-00008
更新日期:2003-08-01 00:00:00
abstract::This study evaluated the effect of three post surface treatments on quartz-fiber post (a) retention and (b) morphology. A control group was not treated. Different treatments were performed for the other posts: silanization; etching by hydrofluoric acid 9.5%; sandblasting with 50 microm Al2O3. Two specimens of each gro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.10.015
更新日期:2007-03-01 00:00:00
abstract::The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200411000-00008
更新日期:2004-11-01 00:00:00
abstract::The purpose of this study was to compare the degree of anesthesia obtained with 2% lidocaine with three different concentrations of epinephrine for inferior alveolar nerve block. Using a repeated measures design, 30 subjects randomly received an inferior alveolar injection using masked cartridges of each solution at t...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/S0099-2399(97)80271-3
更新日期:1997-03-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate porcine palatal mucosa dissolution by sodium hypochlorite (NaOCl) with or without an auxiliary dissolving agent containing glycocholic acid and a mixture of surfactants (Keratobacter [KB]; Saint Joseph DID, Valencia, Spain). METHODS:One hundred forty samples were obta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.01.007
更新日期:2018-05-01 00:00:00
abstract::The aim of this in vivo study was to find a way to conduct qualitative and quantitative evaluations of radicular apical third apposition in rats. Twenty rats were injected intraperitoneally with tetracycline at weight-adjusted doses. Mesio-distal sections of the first mandibular molar regions were prepared. The 60 to ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80153-8
更新日期:1999-04-01 00:00:00
abstract:INTRODUCTION:Apical surgery is frequently indicated in maxillary first molars. Occasionally, a vascular anastomosis in the lateral maxillary sinus wall can be observed during surgery. The aim of this study was to examine the distance between the vascular bone channel (VBC) and the root apices of maxillary first molars ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.09.004
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:A vertical root fracture (VRF), commonly found in teeth with endodontic treatment, is challenging to diagnose and has poor treatment outcomes. Cone-beam computed tomography (CBCT) has become an increasingly popular imaging modality in endodontics, but image artifacts arising from root-filling materials may...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2015.10.005
更新日期:2016-02-01 00:00:00
abstract::The apical sealing ability of amalgam, cold-burnished gutta-percha, amalgam with cavity varnish, and SuperEBA cement was evaluated in extracted human teeth. For each experimental group, the roots of 20 single-rooted teeth were sectioned from their crowns. Canals were cleaned, shaped, and obturated with vertically cond...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80132-3
更新日期:1989-03-01 00:00:00
abstract::The purpose of this study was to compare the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation. Twenty-six bilaterally matched pairs of human teeth were collected. The teeth were incubated with Enterococcus faecalis for 4 weeks. The teeth were divided into two experime...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2006.08.007
更新日期:2007-01-01 00:00:00
abstract::The effects of various cleaning, chemical disinfection, and sterilization procedures on the cutting efficiency of endodontic instruments Unifile (De Trey, Bois Colombes, France), (Flexofile Maillefer, Ballaigues, Switzerland), and H-File (Maillefer)) were investigated. The cross-infection control treatment procedures ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80059-8
更新日期:1996-12-01 00:00:00
abstract::This study evaluated the ability of ultrasonics and calcium hydroxide to remove pulp tissue debris from the mesial root canals of human mandibular molars. All teeth were instrumented using a standard filing technique and irrigated with an equal volume of 2.6% sodium hypochlorite before the application of the experimen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(89)80076-7
更新日期:1989-08-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate the mineral contents of root-canal dentin before and after treatment with two commonly used gutta-percha solvents: chloroform and halothane. Twenty extracted human premolars, whose crowns and apical thirds had been removed, were used. Pulp tissues were removed and the...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200401000-00012
更新日期:2004-01-01 00:00:00
abstract::This study compared the effectiveness of four different techniques used for obturation of single rooted-teeth: lateral condensation, low-temperature gutta-percha (Ultrafil), vertical condensation of gutta-percha softened by means of three different laser devices (argon, CO2, and Nd:YAG), or composite resin photopolyme...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81530-X
更新日期:1995-09-01 00:00:00
abstract::This study examined the effects of calcium-depleting endodontic irrigants, ethylenediamine tetra-acetic acid (EDTA) and BioPure MTAD, on the dissolution, surface characteristics, and ultrastructural characteristics of white mineral trioxide aggregate (MTA). The latter was mixed in a 0.35 water-cement ratio, condensed ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.12.002
更新日期:2007-04-01 00:00:00
abstract:INTRODUCTION:This study aimed to investigate the roles of ephrinB2 in stabilizing vascularlike structures generated by stem cells from apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs). METHODS:HUVECs were seeded alone or with SCAPs concurrently or 12 hours later. Angiogenesis and ephrinB2 pho...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.012
更新日期:2016-09-01 00:00:00
abstract::A method of using osseointegrated implants as an alternative treatment modality for transverse root fractures near the osseous crest is presented. A 15-mm Branemark implant was placed immediately after extraction of a maxillary central incisor with transverse root fracture. Five months after stage I surgery, the impla...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80096-4
更新日期:1998-02-01 00:00:00
abstract:INTRODUCTION:The success of endodontic regeneration lies in the appropriate combination of stem cells and bioactive materials. Several novel dental materials are available on the market in this regard. Hence, the current study aimed to evaluate the proliferation, differentiation, and osteogenic potential of human bone ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.11.016
更新日期:2018-03-01 00:00:00
abstract:INTRODUCTION:The study compared Twisted File (TF) with 3 traditionally manufactured systems to determine whether changes in the manufacturing process improved the cyclic fatigue resistance. METHODS:Four rotary file systems, (1) ProFile (PF), (2) Mtwo, (3) K3, and (4) TF, were tested in artificial canals with 45° and 9...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.12.034
更新日期:2012-04-01 00:00:00
abstract::This review considers oral trauma and its relationship to orthodontics with respect to prevention and primary, secondary, and tertiary care. The level of evidence is not high in regard to this topic, but recommendable approaches to trauma at each stage are discussed on the basis of available literature and published g...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2012.10.030
更新日期:2013-03-01 00:00:00
abstract::Occlusal endodontic access preparations are occasionally made in teeth without removing the original restoration. However, microleakage between restorative materials that are placed at different times has not been extensively studied. Therefore, our objective was to compare microleakage at three areas: between an acce...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80278-7
更新日期:1999-06-01 00:00:00
abstract::The biocompatibility and apical microleakage of tricalcium phosphate based Sankin Apatite (SA) Type I, II, and III root canal sealers were investigated. Teflon tubes containing freshly mixed test materials were implanted in the subcutaneous tissue of mice. The observation periods were 24 h, 7, and 30 days, after which...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(97)80255-5
更新日期:1997-02-01 00:00:00
abstract::The 4- to 6-yr outcome of initial (first-time) endodontic treatment was assessed for Phase II of the "Toronto Study." In total, 442 teeth were treated by using flared preparation and vertical compaction of warm gutta-percha or step-back preparation and lateral compaction. With 126 teeth excluded (discontinuers: deceas...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200405000-00002
更新日期:2004-05-01 00:00:00
abstract:INTRODUCTION:The anatomy of the maxillary C-shaped molar has been the subject of several case reports although no true prevalence research has ever been conducted. The aim of this observational study was to evaluate and characterize the incidence of these morphologies using cone-beam computed tomographic (CBCT) technol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.12.013
更新日期:2016-03-01 00:00:00
abstract::The aim of this review was to consider laboratory experiments and clinical studies of calcium hydroxide-based root canal sealers. An extensive search of the endodontic literature was made to identify publications related to calcium hydroxide-based root canal sealers. The articles were assessed for the outcome of labor...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2008.11.026
更新日期:2009-04-01 00:00:00
abstract::Coconut water is biologically pure and sterile, with a rich presence of amino acids, proteins, vitamins, and minerals. The purpose of this study was to use a collagenase-dispase assay to investigate the potential of a new storage medium, coconut water, in comparison with propolis, Hank's balanced salt solution (HBSS),...
journal_title:Journal of endodontics
pub_type: 杂志文章,收录出版
doi:10.1016/j.joen.2008.01.018
更新日期:2008-05-01 00:00:00
abstract::Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.03.023
更新日期:2006-10-01 00:00:00
abstract:INTRODUCTION:During the dental pulp repair process, human dental pulp cells (HDPCs) migrate to injury sites where they may differentiate into odontoblastlike cells. WNT6 plays a role in dental development and can activate a noncanonical pathway including the c-Jun N-terminal kinase (JNK) pathway. The mechanism of WNT6 ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.12.023
更新日期:2014-07-01 00:00:00
abstract::A new endodontic obturation and retrofilling material has been invented to satisfy Grossman's requirements for an ideal material. Methylene blue dye leakage studies were carried out to compare the new material with conventional products, for both obturation and retrofilling. The experimental material when used in root...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200207000-00008
更新日期:2002-07-01 00:00:00
abstract::In this retrospective study with limited cone-beam computed tomography (limited CBCT), we investigated the anatomic characteristics of the accessory mental foramina and accessory branches of the mandibular canal. The CBCT records of approximately 150 patients were evaluated, and 17 accessory mental foramina were found...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.033
更新日期:2008-12-01 00:00:00