Abstract：
INTRODUCTION:During the dental pulp repair process, human dental pulp cells (HDPCs) migrate to injury sites where they may differentiate into odontoblastlike cells. WNT6 plays a role in dental development and can activate a noncanonical pathway including the c-Jun N-terminal kinase (JNK) pathway. The mechanism of WNT6 in dental pulp repair is still unknown. The purpose of this study was to explore the potential role of the WNT6/JNK signaling pathway in the promotion of cell migration and the differentiation of HDPCs. METHODS:The third passage of HDPCs were cultured in vitro and treated with WNT6 conditioned medium with or without the pretreatment of JNK inhibitor SP600125. The activation of JNK was detected by Western blot, the expression of c-Jun was quantified by reverse-transcription polymerase chain reaction, the migration of HDPCs was determined by wound healing and transwell migration assays, and the differentiation of HDPCs was investigated using alkaline phosphatase staining and alizarin red staining. The expression of odontogenesis-related genes such as Runt-related transcription factor 2, dentin sialophosphoprotein, and dentin matrix protein 1 was quantified. RESULTS:WNT6 activates the JNK pathway in HDPCs and enhances cell migration, mineralization nodule formation, and alkaline phosphatase activation. WNT6 also increases the expression of Runt-related transcription factor 2, dentin sialophosphoprotein, and dentin matrix protein messenger RNA in HDPCs. Blockage of the JNK pathway in HDPCs decreases but does not completely abolish the cell migration and differentiation capacity induced by WNT6. CONCLUSIONS:WNT6 activates the JNK signaling pathway in HDPCs, leading to migration and differentiation.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Li R,Wang C,Tong J,Su Y,Lin Y,Zhou X,Ye Ldoi
10.1016/j.joen.2013.12.023subject
Has Abstractpub_date
2014-07-01 00:00:00pages
943-8issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(13)01168-0journal_volume
40pub_type
杂志文章abstract：:This study compared a tapered, calibrated spreader/plugger instrument (M-series) to noncalibrated spreaders and pluggers in achieving apical condensation of gutta-percha in 20 single-rooted human teeth. The distances between the tip of the instruments and the tip of the master gutta-percha were measured. Results showe...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80680-1
更新日期：1993-04-01 00:00:00
abstract：INTRODUCTION:Instrumentation of the mesial root canal system of mandibular molars may hinder disinfection by packing hard tissue debris within the isthmuses. The removal of accumulated hard tissue debris (AHTD) by 3 supplemental irrigation systems, 2 ultrasonically activated and 1 multisonic, was assessed with micro-co...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.013
更新日期：2019-07-01 00:00:00
abstract：:The purpose of this study was to compare ultrasonic and high-speed-bur root-end preparations. Seventy-six roots from 29 bilaterally matched pairs of human teeth in cadavers were used in this study. In group 1 ultrasonic preparations were made in 38 roots and filled with amalgam. In group 2 high-speed bur preparations ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(97)80301-9
更新日期：1997-07-01 00:00:00
abstract：INTRODUCTION:This study aimed to evaluate the frequency of dentinal microcracks observed after root canal preparation with 2 reciprocating and a conventional full-sequence rotary system using micro-computed tomographic analysis. METHODS:Thirty mesial roots of mandibular molars presenting a type II Vertucci canal confi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.02.019
更新日期：2014-09-01 00:00:00
abstract：:The aims of this study were to compare Resilon (Resilon Research LLC, Madison, CT) in conjunction with either a bonding (Epiphany; Pentron Clinical Technologies, Wallingford, CT) or a nonbonding endodontic sealer (Pulp Canal Sealer; Kerr Corporation, Orange, CA) to EndoRez (Ultradent Products Inc, South Jordan, UT) an...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.02.018
更新日期：2007-07-01 00:00:00
abstract：:Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study,...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200308000-00008
更新日期：2003-08-01 00:00:00
abstract：:Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80959-3
更新日期：1992-06-01 00:00:00
abstract：INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.03.006
更新日期：2014-09-01 00:00:00
abstract：:This study compares the incidence, degree, and length of postoperative pain in 300 endodontically treated teeth, with and without apical patency, in relation to some diagnostic factors (vitality, presence of preoperative pain, group, and mandible of treated tooth). Of the questionnaires received back, apical patency w...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2008.11.014
更新日期：2009-02-01 00:00:00
abstract：INTRODUCTION:This study aimed to evaluate the precision of electronic apex locators (EALs) in locating the apical constriction (AC) during a root canal treatment compared with a histologic evaluation of the AC as well as the effects of possible influencing factors by means of a systematic review of the literature and m...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joen.2015.08.012
更新日期：2015-11-01 00:00:00
abstract：:One hundred single-rooted teeth were instrumented with Lightspeed, Profile .04 Taper Series 29, and NT McXIM instruments according to the recommendations of the manufacturers, and Flex-R files using the balanced force technique. Groups were comparable with respect to average curvature, canal length, and major and mino...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80086-1
更新日期：1998-02-01 00:00:00
abstract：:The diameter of nine sizes of three brands of endodontic spreaders was measured at D1 and D16 and compared with corresponding sizes of six brands of accessory gutta-percha cones. Two evaluators independently measured 20 randomly selected samples of each size of finger spreader and accessory gutta-percha cone. A standa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(07)80153-1
更新日期：1991-01-01 00:00:00
abstract：:Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200306000-00011
更新日期：2003-06-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of low-surface tension EDTA solutions on the push-out bond strength of resin-based sealer to young and old root canal dentin. METHODS:Root canals from 64 (n = 32 age <30 years old and n = 32 >60 years old) extracted, decoronized, single-rooted human tee...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.09.007
更新日期：2018-03-01 00:00:00
abstract：:Apical periodontitis was induced in Wistar rats by exposing the pulp chamber of right mandibular first molars to the oral environment. Animals were killed 0, 5, 10, 15, 20, 30, 60, and 80 days after lesion induction. Microradiographic and automated image analysis showed that the lesions expanded significantly in a tim...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200006000-00007
更新日期：2000-06-01 00:00:00
abstract：:The cyclic fatigue resistance of three types of nickel-titanium rotary files was compared in a model using reciprocating axial movement. The influence of file size and taper was also investigated and fracture patterns were examined under SEM. The 10 experimental groups consisted of ProFiles, K3s, and RaCe files, size ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.013
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.006
更新日期：2019-01-01 00:00:00
abstract：:The purpose of the study was to evaluate the biocompatibility of two current adhesive resins and a calcium hydroxide cement. Fifty-four polyethylene tubes were filled with these dental materials, which were hand-mixed or light-cured according to the manufacturer's directions: group 1--Clearfill Liner Bond 2 (Kuraray);...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200009000-00006
更新日期：2000-09-01 00:00:00
abstract：:This report presents a rare case of odontogenic cutaneous sinus tract caused by a vertical root fracture. The root-fractured molar was probably caused by an accidental blow by a cow 3 years ago. Despite all treatments by physicians and surgeons for nearly 1 year, the sinus tract recurred repeatedly. After diagnosis an...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/s0099-2399(06)81130-1
更新日期：1997-09-01 00:00:00
abstract：:Seventy-three teeth with acute irreversible pulpitis were subjected to emergency pulpotomies. After removal of the coronal portion of the pulp, a sterile cotton pellet or zinc oxide-eugenol cement was placed against the remaining pulp tissue. The cotton pellet was either dry or moistened with camphorated phenol, cresa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80219-5
更新日期：1989-06-01 00:00:00
abstract：:A method is proposed for a three-dimensional visualization of the root canal system before and after the cleaning and shaping procedure to analyze the relationships of the root canal walls. The first part of the study provided cross-sections of the root perpendicular to the root canal. A system for remounting the sect...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81298-7
更新日期：1993-05-01 00:00:00
abstract：:This case-control study sought to identify variables predictive of subsequent root canal therapy (RCT) in teeth receiving full coverage restorations. The University of North Carolina School of Dentistry's computerized treatment database was used to identify all patients receiving a single-unit crown on a nonendodontic...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.11.001
更新日期：2006-08-01 00:00:00
abstract：:Root resorption after replantation of mature permanent dog incisors was examined by scanning electron microscopy. Three groups consisting of three extracted teeth each were kept in a saline solution for 5, 30, and 120 min before replantation. The first group was replanted with the pulp intact. The second and third gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80996-9
更新日期：1995-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the variations in root canal length (RCL) occurring during endodontic treatment stages (initial, preflared, and concluded) and correlate them with the accuracy of Root ZX II (RZX). METHODS:After coronal access, 26 mandibular molars had the apical foramen of the 52 me...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.07.022
更新日期：2016-11-01 00:00:00
abstract：INTRODUCTION:This retrospective study assessed the effects of microsurgical treatment of post-treatment endodontic disease using SuperEBA (Bosworth, Skokie, IL) as the root-end filling material and evaluated the potential prognostic factors in relation to outcome. METHODS:Data were collected from patients diagnosed wi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.11.001
更新日期：2014-03-01 00:00:00
abstract：INTRODUCTION:Root resorption is a common complication after replantation following traumatic dental avulsion. Endodontic therapy combined with local and intracanal medications aims to avoid osteoclastic activity. In such cases, the application of alendronate (ALN), a bisphosphonate widely used for the treatment of bone...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.010
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. METHODS:After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.10.002
更新日期：2012-02-01 00:00:00
abstract：INTRODUCTION:A great number of studies performed in the last 3 decades have shown the superior mechanical features of nickel-titanium files as compared with stainless steel files. A novel file system recently developed, Gentlefile, defies these findings and claims to have superior qualities despite the fact it is made ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.12.023
更新日期：2016-03-01 00:00:00
abstract：:Whole genome DNA-DNA hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. There are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-re...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200407000-00014
更新日期：2004-07-01 00:00:00
abstract：INTRODUCTION:The root canal treatment of a mandibular molar with aberrant canal configuration can be diagnostically and technically challenging. METHODS:This case report presents the clinical management of a mandibular first molar with three separate mesial canals including middle mesial canal, which was confirmed by ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.008
更新日期：2010-03-01 00:00:00
