Abstract：
INTRODUCTION:Root resorption is a common complication after replantation following traumatic dental avulsion. Endodontic therapy combined with local and intracanal medications aims to avoid osteoclastic activity. In such cases, the application of alendronate (ALN), a bisphosphonate widely used for the treatment of bone disorders, could be of clinical relevance. This study evaluated alendronate biocompatibility on periodontal ligament cells as well as its effects on an in vitro osteoclastogenesis model. METHODS:Alendronate cytotoxicity (10(-3) to 10(-9) mol/L) in human periodontal ligament fibroblasts, human osteogenic sarcoma cells, and murine osteoclastic precursors (RAW 264.7) was analyzed using cell number determination, cell viability, and proliferation assays. ALN (10(-6) to 10(-12) mol/L) effects on RANKL-induced osteoclastogenesis of RAW cells were assessed by tartrate-resistant acid phosphatase (TRAP) staining and activity and real-time polymerase chain reaction. RESULTS:ALN at higher concentrations was cytotoxic for all cell types, inhibiting significantly the proliferation of human osteogenic sarcoma cells and human periodontal ligament fibroblasts (≥10(-5) mol/L). TRAP activity and expression of the osteoclast markers TRAP and cathepsin K by RAW-derived osteoclasts decreased significantly with ALN at low concentrations, reaching the maximum effect at 10(-10) mol/L. CONCLUSIONS:We showed that ALN at very low concentrations is an effective inhibitor of RANKL-generated osteoclasts, without causing cytotoxic effects on their precursors or periapical cells. ALN at such concentrations might be useful to prevent replacement resorption in avulsed teeth.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Martins CA,Leyhausen G,Volk J,Geurtsen Wdoi
10.1016/j.joen.2014.07.010subject
Has Abstractpub_date
2015-01-01 00:00:00pages
45-9issue
1eissn
0099-2399issn
1878-3554pii
S0099-2399(14)00644-Xjournal_volume
41pub_type
杂志文章abstract：INTRODUCTION:The aims of this study were to examine the immunolocalization of protein phosphatase 1 (PP1) in developing mouse pulp tissue and to explore the role of PP1 in odontoblastic differentiation and in vitro angiogenesis in human dental pulp cells (HDPCs). METHODS:Immunolocalization of PP1 was assessed in devel...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.013
更新日期：2017-03-01 00:00:00
abstract：INTRODUCTION:Previous studies in patients with irreversible pulpitis have reported increased success of the inferior alveolar nerve block (IANB) using premedication with ketorolac. Preemptive nitrous oxide administration has also shown an increase in the success of the IANB. Recently, ketorolac has been made available ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.09.011
更新日期：2018-01-01 00:00:00
abstract：:The purpose of this in vitro study was to investigate the canal configuration types, and the prevalence and location of anatomical variations in the mesiobuccal (MB) and mesial roots of permanent maxillary and mandibular first molars after instrumentation. The number and the type of canals were determined before instr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000145425.73364.91
更新日期：2005-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effect of a range of alkaline pH values on the push-out strength of white mineral trioxide aggregate (WMTA). METHODS:The standardized lumens of root slices prepared from extracted single-rooted human teeth were filled with white ProRoot MTA. The specimens were the...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2010.08.022
更新日期：2010-11-01 00:00:00
abstract：INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：INTRODUCTION:This article reports on the feasibility of conducting a large-scale endodontic prospective cohort study in The National Dental Practice-Based Research Network. This study was designed to measure pain and burden associated with initial orthograde root canal therapy (RCT) and to explore potential prognostic ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.08.002
更新日期：2012-11-01 00:00:00
abstract：:In this study, we examined the effects of TNF-alpha on Bone morphogenetic protein (BMP-2), Smads (which play intracellular signaling of BMPs) expression and alkaline phosphatase (ALP) activity of human dental pulp (HDP) cells to clarify the mechanism of tertiary dentin formation. The quantity of RT-PCR product for BMP...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.12.007
更新日期：2006-06-01 00:00:00
abstract：:This in vitro study evaluated the survival rate and fracture resistance of maxillary central incisors restored with different post and core systems. The post and core systems investigated were a prefabricated high precious metal post with cast core (group A), zirconia post with a prefabricated bonded ceramic core (gro...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200210000-00008
更新日期：2002-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this systematic review was to compare the clinical and radiographic outcomes of nonsurgical retreatment with those of endodontic surgery to determine which modality offers more favorable outcomes. METHODS:The study began with targeted electronic searches of MEDLINE, PubMed, and Cochrane dat...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joen.2009.04.023
更新日期：2009-07-01 00:00:00
abstract：INTRODUCTION:This retrospective study aimed to analyze the effect of repairing endodontic access cavities with composite on the survival of single crowns and retainer restorations of fixed or removable dental prostheses. METHODS:Dental records of patients attending a university dental clinic were retrospectively scree...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.11.012
更新日期：2020-03-01 00:00:00
abstract：INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.09.008
更新日期：2019-12-01 00:00:00
abstract：:The sealing properties of three temporary restorative materials, Cavit, IRM, and a polycarboxylate-based cement, Ultratemp Firm, were investigated in vitro. Standardized access cavities were prepared in 45, intact, extracted, human molars. The teeth were randomly assigned to three groups and the access openings filled...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/01.don.0000121610.63000.f2
更新日期：2004-08-01 00:00:00
abstract：:The purpose of this study was to compare the wicking propensity of multifilament sutures. Dexon II, Vicryl, and black silk suture (BSS) were dipped in saline or soaked for 48 h, then suspended on a microscope slide. Fluorescein isothiocyanate-dextran (FITC-D) was placed at the suture mid points, and its movement was o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000121617.67923.05
更新日期：2004-09-01 00:00:00
abstract：:In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(96)80285-8
更新日期：1996-03-01 00:00:00
abstract：:Whole genome DNA-DNA hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. There are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-re...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200407000-00014
更新日期：2004-07-01 00:00:00
abstract：:The authors reviewed the literature evaluating the antibacterial effectiveness of intracanal medicaments used in the management of apical periodontitis. A PICO (problem, intervention, comparison, outcome) strategy was developed to identify studies dealing with calcium hydroxide, phenolic derivatives, iodine-potassium ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000129959.20011.ee
更新日期：2004-10-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate and compare the antimicrobial activity of calcium hydroxide, triantibiotic mixture (TAM), and an ethanol extract of propolis as intracanal medicaments on Enterococcus faecalis-infected root canals. METHODS:One hundred twenty extracted intact human permanent incisors w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.05.028
更新日期：2011-09-01 00:00:00
abstract：:The purpose of this study was to compare ultrasonic and high-speed-bur root-end preparations. Seventy-six roots from 29 bilaterally matched pairs of human teeth in cadavers were used in this study. In group 1 ultrasonic preparations were made in 38 roots and filled with amalgam. In group 2 high-speed bur preparations ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(97)80301-9
更新日期：1997-07-01 00:00:00
abstract：:The purpose of this study was to examine the effect of the smear layer on apical microleakage over 16 wk. One hundred and four extracted human teeth were assigned to four groups treated as follows: group A1--smear layer was left intact and canals were obturated with gutta-percha and Roth 811; group A2--smear layer was...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80010-7
更新日期：1999-02-01 00:00:00
abstract：:The apical sealing ability of amalgam, cold-burnished gutta-percha, amalgam with cavity varnish, and SuperEBA cement was evaluated in extracted human teeth. For each experimental group, the roots of 20 single-rooted teeth were sectioned from their crowns. Canals were cleaned, shaped, and obturated with vertically cond...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80132-3
更新日期：1989-03-01 00:00:00
abstract：:Simulated furcation perforations were repaired with and without blood contamination utilizing tooth-colored mineral trioxide aggregrate (MTA) mixed with either MTA liquid (sterile water), lidocaine, or saline. Samples underwent Instron testing at either 24 or 72 hours and at 7 days. Data were analyzed using a 3-way AN...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.09.007
更新日期：2006-05-01 00:00:00
abstract：INTRODUCTION:This study aimed to analyze the external and internal morphologies of 3-rooted maxillary first premolars using cone-beam computed tomographic (CBCT) imaging. METHODS:Fifty-six three-rooted maxillary first premolars were imaged by CBCT imaging and classified into 4 groups on the basis of external root morp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.045
更新日期：2017-08-01 00:00:00
abstract：INTRODUCTION:In this study, we investigated if apical periodontitis (AP) associated with diabetes influenced the levels of endogenous antioxidants, the total antioxidant capacity (TAC), and the oxidant parameter in the serum of Wistar rats. METHODS:Forty male rats were divided into 4 equal groups: normal rats (N), rat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.05.014
更新日期：2017-10-01 00:00:00
abstract：INTRODUCTION:Many studies have investigated the morphology of the mandibular molar, but the prevalence of the middle mesial (MM) canal in the mesial root of the mandibular molar is still the subject of controversy. In addition, in previous literature, a true MM canal has not been clearly distinguished from an isthmus b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.008
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:This review aimed to find the most effective oral premedication in reducing pain in adults after nonsurgical root canal therapy (NSRCT) using network meta-analysis. METHODS:The review protocol was registered in the PROSPERO database (CRD42017071899). A literature search was performed in the MEDLINE and EB...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2018.10.016
更新日期：2019-04-01 00:00:00
abstract：INTRODUCTION:The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation. METHODS:One hundred twelve dentin bloc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.018
更新日期：2018-11-01 00:00:00
abstract：:The oral region comprises 1% of the total body area, yet it accounts for 5% of all bodily injuries. In preschool children, oral injuries make up as much as 17% of all bodily injuries. The incidence of traumatic dental injuries is 1%-3%, and the prevalence is steady at 20%-30%. The annual cost of treatment is US $2-$5 ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2012.11.021
更新日期：2013-03-01 00:00:00
abstract：:This report examines three clinical cases of root-filled teeth with primary endodontic lesions. During endodontic surgery, an area of incomplete root bifurcation beginning in the coronal or middle third of the root surface and terminating at or near the apical foramen were observed. The radicular grooves of two teeth ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200005000-00012
更新日期：2000-05-01 00:00:00
abstract：INTRODUCTION:We evaluated the radiopacity and antibacterial properties of various concentrations of double antibiotic paste (DAP) containing barium sulfate (BaSO4) or zirconium oxide (ZrO2) radiopaque agents. METHODS:The radiopacity of 1, 10, and 25 mg/mL DAP containing 30% (w/v) BaSO4 or ZrO2, DAP-free radiopaque pas...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.06.010
更新日期：2018-09-01 00:00:00
abstract：:The cleaning capacity of manual and rotary instrumentation techniques in mesial-distal flattened canals was studied by morphometric analysis. Twenty human mandibular incisors were divided into two groups of 10 teeth each: group 1, crown-down technique with rotary instrumentation using ProFile .04; group 2, crown-down ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200205000-00004
更新日期：2002-05-01 00:00:00
