Abstract:
:In this study, we examined the effects of TNF-alpha on Bone morphogenetic protein (BMP-2), Smads (which play intracellular signaling of BMPs) expression and alkaline phosphatase (ALP) activity of human dental pulp (HDP) cells to clarify the mechanism of tertiary dentin formation. The quantity of RT-PCR product for BMP-2 from the HDP cells stimulated by TNF-alpha is increased. However, ALP activity was not increased on the cells incubated with TNF-alpha. On the other hand, ALP activity was significantly increased on HDP cells treated with Ammonium Pyrrolidinedithiocarbamate (PDTC, NF- kappaB inhibitor) groups and combined supplementation of TNF-alpha and PDTC groups. Furthermore, we examined the effect of TNF-alpha and PDTC on Smad7 expression using RT-PCR and western blot analysis. Smad7 expression in HDP cells was increased by TNF-alpha, but decreased by PDTC treatment. These results suggest that NF- kappaB and Smad7 play an important role in the down regulation of ALP activity by TNF-alpha on HDP cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Okabe T,Matsushima Kdoi
10.1016/j.joen.2005.12.007subject
Has Abstractpub_date
2006-06-01 00:00:00pages
516-20issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(05)00183-4journal_volume
32pub_type
杂志文章abstract:INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.016
更新日期:2011-11-01 00:00:00
abstract:INTRODUCTION:Endodontic claims are the most frequently filed malpractice claims in dentistry. The aim of this study was to categorize and review errors and complications related to endodontic procedures that resulted in legal actions against the treating practitioner. METHODS:All dental malpractice complaints reported...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.03.030
更新日期:2010-06-01 00:00:00
abstract:INTRODUCTION:Incision and drainage of symptomatic emergency patients with facial swelling is painful even after local anesthetics are administered. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and the pain of an incision and drainage procedure of a buffered ver...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.06.017
更新日期:2015-10-01 00:00:00
abstract::Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200301000-00013
更新日期:2003-01-01 00:00:00
abstract::The cyclic fatigue resistance of three types of nickel-titanium rotary files was compared in a model using reciprocating axial movement. The influence of file size and taper was also investigated and fracture patterns were examined under SEM. The 10 experimental groups consisted of ProFiles, K3s, and RaCe files, size ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.013
更新日期:2006-01-01 00:00:00
abstract:INTRODUCTION:Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.03.006
更新日期:2018-06-01 00:00:00
abstract::Enterococcus faecalis, a pathogenic gram-positive bacterium, is closely related to refractory apical periodontitis. Because lipoteichoic acid (LTA) is considered a major virulence factor of gram-positive bacteria, in the present study, highly pure LTA from E. faecalis was prepared, and its ability to stimulate murine ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.05.005
更新日期:2008-08-01 00:00:00
abstract::With the recent influx of osseointegrated endosseous implants in dentistry, attention has been drawn away from using endodontic stabilizers as a means of stabilizing and retaining teeth. It is our opinion that endodontic stabilizers should be considered in the treatment planning of seemingly nonretainable teeth. They ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81268-9
更新日期:1992-05-01 00:00:00
abstract::This study investigated the possibility of saliva recontamination occurring between the root canal wall and sealer through dentinal tubules exposed after the cementum was removed at the cervical level by root planing and treatment with citric acid. Thirty-four extracted human maxillary anterior teeth were randomly pla...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80024-0
更新日期:1996-11-01 00:00:00
abstract::This report examines three clinical cases of root-filled teeth with primary endodontic lesions. During endodontic surgery, an area of incomplete root bifurcation beginning in the coronal or middle third of the root surface and terminating at or near the apical foramen were observed. The radicular grooves of two teeth ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200005000-00012
更新日期:2000-05-01 00:00:00
abstract:INTRODUCTION:The purposes of this retrospective study were to evaluate the clinical and radiographic outcomes of mineral trioxide aggregate apexification and revascularization in nonvital immature permanent teeth and to analyze factors influencing treatment outcome. METHODS:Forty-six cases (29 cases of apexification a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.030
更新日期:2017-02-01 00:00:00
abstract::Retrograde (or apical/periapical) peri-implantitis (RPI) presents with radiographic signs of bone loss at the periapical area of endosteal implants and may also present with clinical signs of abscess formation or a sinus tract traceable to the implant periapex. The lesion may form days up to several years after the in...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.01.002
更新日期:2019-05-01 00:00:00
abstract::Little is known about the effect of aging on characteristic functions of pulp cells. When damaged pulp is recovered and mineralized tissue is formed to protect remaining pulp tissue, the general responses of pulp tissue after adequate stimuli (pulp cell proliferation and activation of alkaline phosphatase [ALPase]) ar...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200301000-00003
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate and compare the resistance to cyclic fatigue of the new One Curve (OC) instrument in double (S-shaped) and single curvature canals compared with other nickel-titanium rotary instruments. METHODS:Size 25/.06 of OC, 2Shape (TS), Vortex Blue (VB), ProFile Vortex (PV), an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.07.023
更新日期:2018-11-01 00:00:00
abstract::A technique utilizing fiberoptics to transilluminate a beveled root surface during periradicular surgery is described. This procedure when integrated with other standard endodontic surgical aids may help diagnose apical variations and vertical root fractures. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80301-8
更新日期:1994-08-01 00:00:00
abstract::Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2014.01.017
更新日期:2014-04-01 00:00:00
abstract::The antimicrobial effect of RC-Prep and its components--10% urea peroxide, 15% EDTA, and glycol--were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent fo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80296-9
更新日期:1999-12-01 00:00:00
abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.024
更新日期:2017-09-01 00:00:00
abstract::The mesiobuccal roots of 50 randomly selected maxillary first molars were examined to assess the incidence and position of the canal isthmus. Transverse serial sections of the apical 6 mm of each root were prepared in 1-mm increments. The apical side of each section was stained with methylene blue dye, viewed with a s...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80975-1
更新日期:1995-07-01 00:00:00
abstract:INTRODUCTION:The study was designed as a randomized double-blind trial to evaluate the anesthetic efficacy of 4% articaine with 1:100,000 epinephrine in inferior alveolar nerve block (IANB) and infiltration anesthetic techniques to anesthetize mandibular molars with irreversible pulpitis. METHODS:The study was compose...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2011.09.009
更新日期:2011-12-01 00:00:00
abstract::The aims of this study were to identify microorganisms on the surface of gutta-percha (GP) cones using a polymerase chain reaction (PCR) and to evaluate the sterilization effect of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and ChloraPrep on these bacteria. Alterations of the surface texture and physic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.01.019
更新日期:2007-05-01 00:00:00
abstract::In the past, measurement of microleakage has been based on measurement of dye or radioactive isotope penetration. A method has been developed to allow electronic monitoring of microflow in the same specimen over a period of time. This method was used to compare previously reported retrograde materials with an amalgam/...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80072-5
更新日期:1994-11-01 00:00:00
abstract::Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200110000-00004
更新日期:2001-10-01 00:00:00
abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(96)80285-8
更新日期:1996-03-01 00:00:00
abstract:INTRODUCTION:The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. T...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.016
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:This in vitro study compared cone-beam computed tomography (CBCT) exam with different voxel sizes with digital periapical radiography in the detection of vertical root fractures in teeth with and without intracanal metallic posts. METHODS:Eighteen single-rooted human teeth were endodontically treated, pre...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.031
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:The morphogenic complexities of dens invaginatus (DI) Oehlers type 3 in maxillary canines offer significant endodontic challenges. METHODS:A case report is provided of a 14-year-old female patient who presented with an anomalous-looking permanent maxillary canine associated with a sinus tract. Pulp testin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.06.008
更新日期:2014-10-01 00:00:00
abstract::The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.009
更新日期:2018-05-01 00:00:00
abstract::The aim of this study was to determine the relationship between histologic and radiologic signs of inflammation in human root-filled teeth. In addition, other factors with possible importance for apical inflammation were assessed. Fifty-three block sections of root-filled teeth were gathered from human cadavers. The b...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200402000-00003
更新日期:2004-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this systematic review was to compare the clinical and radiographic outcomes of nonsurgical retreatment with those of endodontic surgery to determine which modality offers more favorable outcomes. METHODS:The study began with targeted electronic searches of MEDLINE, PubMed, and Cochrane dat...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.joen.2009.04.023
更新日期:2009-07-01 00:00:00