Abstract:
INTRODUCTION:The aims of this study were to examine the immunolocalization of protein phosphatase 1 (PP1) in developing mouse pulp tissue and to explore the role of PP1 in odontoblastic differentiation and in vitro angiogenesis in human dental pulp cells (HDPCs). METHODS:Immunolocalization of PP1 was assessed in developing mouse pulp tissue. Odontogenic differentiation was examined by alkaline phosphatase activity, alizarin red staining, and reverse transcriptase polymerase chain reaction. Angiogenesis was evaluated by endothelial cell migration and capillary tube formation. Signaling pathways were analyzed by Western blotting and confocal immunofluorescence. RESULTS:PP1 expression was detected in preodontoblasts, odontoblasts, dental pulp cells, and endothelial cells within pulp tissue during the crown formed, root formation, and root completion stages. PP1 messenger RNA (mRNA) and protein levels were up-regulated at the late mineralization stage during odontogenic differentiation of HDPCs. The PP1 activator C2 ceramide increased alkaline phosphatase activity, mineralized nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibited odontoblastic differentiation. Moreover, PP1 activator up-regulated mRNA expression of angiogenic genes in HDPCs and increased the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA showed opposite effects. C2 ceramide increased levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix. CONCLUSIONS:This study provides the first evidence that PP1 might be a potent regulator of developing pulp tissue in vivo and odontoblastic differentiation and angiogenesis in HDPCs in vitro and may have clinical implications for pulp/dentin regeneration or reparative dentinogenesis.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Kim JY,Kim DS,Auh QS,Yi JK,Moon SU,Kim ECdoi
10.1016/j.joen.2016.10.013subject
Has Abstractpub_date
2017-03-01 00:00:00pages
417-424issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(16)30740-3journal_volume
43pub_type
杂志文章abstract::With all the potential for endodontic success, the fact remains clinicians are confronted with posttreatment endodontic disease. When the elected treatment plan is endodontic nonsurgical retreatment, then the goal is to access the pulp chamber and remove materials from the root canal space and if present, address defi...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1097/01.don.0000145033.15701.2d
更新日期:2004-12-01 00:00:00
abstract:INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.07.016
更新日期:2018-11-01 00:00:00
abstract::This study assessed in vitro the diagnostic ability of cone beam computed tomography (CBCT) scans with different voxel resolutions in the detection of simulated external root resorption (ERR). For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.005
更新日期:2009-02-01 00:00:00
abstract::Inflammation of pulp tissue appears as a consequence of caries progression. Its main characteristic is inflamed infiltrate whose cells contain lymphocytes. CD44 is a widely expressed adhesion molecule present in several body cells such as leukocytes and parenchymatous cells, including endothelial cells, epithelial cel...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.05.022
更新日期:2007-10-01 00:00:00
abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80460-7
更新日期:1993-06-01 00:00:00
abstract:INTRODUCTION:The purpose of this investigation was to determine the effect that apical preparation size and preparation taper have on the volume of irrigant delivered at the working length for different canal curvatures using apical negative pressure irrigation. METHODS:One hundred fifty-five human teeth (55 maxillary...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.10.008
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:The aim of this prospective study was to evaluate the postoperative pain that followed root canal treatments performed with a single-file reciprocating system on asymptomatic uniradicular necrotic teeth with and without foraminal enlargements (FEs). METHODS:Forty-six volunteers were randomly divided into ...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.09.018
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:This study evaluated the cyclic fatigue resistance (CFR) of Reciproc (R25 and R40; VDW, Munich, Germany) and Reciproc Blue (R25 and R40, VDW) instruments used in an artificial S-shaped canal. METHODS:A total of 80 files were tested in an S-shaped canal (n = 20 for each file, Reciproc R25 and R40 and Recip...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.009
更新日期:2017-10-01 00:00:00
abstract:INTRODUCTION:Teeth undergoing initial endodontic therapy have a very high survival rate. Some teeth that continue to show signs of pathosis after the initial therapy will require nonsurgical (orthograde) retreatment. Outcome assessment of endodontic retreatment is crucial for appropriate case selection and treatment pl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.02.009
更新日期:2010-05-01 00:00:00
abstract:INTRODUCTION:Buffered local anesthetics have not been studied for incision and drainage procedures in dentistry. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and pain of an incision and drainage procedure by using a buffered versus a nonbuffered 2% lidocaine wi...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2013.07.008
更新日期:2013-11-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to compare the outcomes of initial nonsurgical root canal therapy for different tooth types provided by both endodontists and other providers. METHODS:By using an insurance company database, 487,476 initial nonsurgical root canal therapy procedures were followed from the ti...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.02.008
更新日期:2016-05-01 00:00:00
abstract::Periapical lesions usually are caused by root canal infection; nevertheless, other pathologies may eventually involve the tooth apex, making the correct diagnosis more difficult. Glandular odontogenic cysts (GOCs) are uncommon and, despite their cystic nature, may present an aggressive behavior and a high recurrence r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.06.015
更新日期:2017-11-01 00:00:00
abstract:INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.09.020
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:The direct effect of follicle-stimulating hormone (FSH) on bone resorption has been demonstrated recently. The aim of this study was to evaluate the effect of FSH inhibitor leuprorelin (LE) on alveolar bone loss resulting from experimental periapical lesions in ovariectomized (OVX) rats. METHODS:Twenty-se...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.01.011
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:Outcome studies of endodontic treatment of necrotic immature permanent teeth rely on radiographic measures as surrogates of whether the treatment achieved regeneration/revascularization/revitalization. An increase in radiographic root length and/or width is thought to result in a better long-term prognosis...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.025
更新日期:2014-01-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the volume of removed dentin, transportation, and centering ability of ProTaper Next (PTN) system with and without glide path preparation by using cone-beam computed tomography (CBCT) imaging. METHODS:Sixty mesiobuccal canals of mandibular first molars...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.09.001
更新日期:2014-12-01 00:00:00
abstract::Unlike most cases of combined orthodontic and endodontic therapy that have been reported, in this case endodontic treatment with calcium hydroxide-induced calcification was utilized prior to orthodontic treatment. A 14-yr-old female required orthodontic therapy due to anterior crowding. Preoperative periapical radiogr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80290-6
更新日期:1994-05-01 00:00:00
abstract::The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200403000-00004
更新日期:2004-03-01 00:00:00
abstract::Two electronic apex locators based on the gradient (Apit) and absolute (Odontometer) impedance principles were evaluated. Distances of file tips to apical foramina were determined in vivo with the electronic apex locators and subsequently verified after extraction. Mean differences between file tips and apical foramin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80145-7
更新日期:1996-05-01 00:00:00
abstract:INTRODUCTION:This in vitro study compared 3 agitation and 2 irrigation devices to ultrasonic agitation at mechanically removing bacteria from a plastic simulated canal, instrumented to 35/.06. METHODS:The plastic blocks were divided into seven groups. The control (C) group with brain-heart infusion (BHI) broth (steril...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.04.007
更新日期:2009-07-01 00:00:00
abstract::The biocompatibility and apical microleakage of tricalcium phosphate based Sankin Apatite (SA) Type I, II, and III root canal sealers were investigated. Teflon tubes containing freshly mixed test materials were implanted in the subcutaneous tissue of mice. The observation periods were 24 h, 7, and 30 days, after which...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(97)80255-5
更新日期:1997-02-01 00:00:00
abstract:INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.007
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate and compare the antimicrobial activity of calcium hydroxide, triantibiotic mixture (TAM), and an ethanol extract of propolis as intracanal medicaments on Enterococcus faecalis-infected root canals. METHODS:One hundred twenty extracted intact human permanent incisors w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.028
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:Bleeding control is an important aspect in endodontic surgery. Two hemostatic techniques were compared with regard to their efficacy to bleeding control in endodontic surgery. METHODS:A randomized, 2-arm, parallel pilot study involving 30 patients with periradicular lesions was performed including the fol...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2019.04.011
更新日期:2019-08-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the tooth structure theoretically required to be removed in the coronal and cervical regions to accomplish 3 different endodontic access preparations in a novel digital model. The deviation of the center of access cavity from the central fossa will also be measured...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.01.002
更新日期:2020-04-01 00:00:00
abstract:INTRODUCTION:The alterations in dentin tissue depending on increasing age might cause different adhesion capability of bacteria, yielding differences in clinical approaches regarding root canal irrigation. This study, therefore, aimed to evaluate the effects of ethylenediaminetetraacetic acid (EDTA) and sodium hypochlo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.01.008
更新日期:2010-05-01 00:00:00
abstract::The inferior alveolar nerve is sometimes affected by periapical pathoses and mandibular cysts. However, mandibular intraosseous lesions have not been reported to disturb the lingual nerve. A case of simultaneous paresthesia of the right lingual nerve and the right inferior alveolar nerve is presented. The possible mec...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000158011.64829.7b
更新日期:2005-10-01 00:00:00
abstract:INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the prolifera...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.01.038
更新日期:2015-06-01 00:00:00
abstract:INTRODUCTION:Dental pulp has limited capability to regenerate, which happens in the early stage of pulpitis. An ambiguous relationship exists; inflammation may impair or support pulp regeneration. Epigenetics, which is involved in cell proliferation and inflammation, could regulate human dental pulp cell (HDPCs) regene...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.031
更新日期:2014-08-01 00:00:00
abstract::This in vitro study compared the accuracy of file length measurements made on calibrated and uncalibrated direct digital images. Endodontic files of known lengths and ISO sizes were used in 10 single-rooted, relatively straight teeth within cadaver specimens. The crowns of the teeth were ground flat and an orthodontic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200112000-00017
更新日期:2001-12-01 00:00:00