Effects of sodium tri- and hexametaphosphate on proliferation, differentiation, and angiogenic potential of human dental pulp cells.

Abstract:

INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the proliferation, odontoblastic differentiation, and angiogenic potential of human dental pulp cells (HDPCs). METHODS:Differentiation was measured by alkaline phosphatase activity, calcified nodule formation by alizarin red staining, and marker messenger RNA (mRNA) levels by reverse-transcription polymerase chain reaction. In vitro angiogenesis was quantified by migration, mRNA levels of angiogenic genes, and endothelial tube formation. RESULTS:STP and SHMP dose dependently increased the proliferation and ALP activity and enhanced mineralized nodule formation and odontoblast marker mRNAs of HDPCs. STP and SHMP resulted in the up-regulation of angiogenic genes in HDPCs. Endothelial cells treated with conditioned medium collected from STP- and SHMP-exposed HDPCs showed an increase in migration and capillary tube formation. Knockdown of the expression of the genes encoding of inorganic pyrophosphate by small interfering RNA attenuated the STP- and SHMP-induced odontogenic differentiation and angiogenic potential. CONCLUSIONS:This study showed that STP and SHMP promote the growth, differentiation, and angiogenic potential of HDPCs. These results suggest that STP and SHMP may be candidates for dental pulp tissue engineering and regenerative endodontics.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Bae WJ,Jue SS,Kim SY,Moon JH,Kim EC

doi

10.1016/j.joen.2015.01.038

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

896-902

issue

6

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(15)00105-3

journal_volume

41

pub_type

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