Abstract:
INTRODUCTION:In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating inflammasome with apoptosis-associated speck-like protein containing a caspase activating recruiting domain. In this study, we determined whether AIM2 was expressed in pulp cells and defined the role of AIM2 in the initiation of inflammation within the dental pulp. METHODS:In the in vivo study, the right maxillary molars from male adult Sprague-Dawley rats (250-350 g) were exposed to the pulp. In the in vitro study, the pulp cells isolated from the mandibular incisors of the Sprague-Dawley rats (2 weeks) were conventionally cultured. Immunofluorescence staining was used to determine the expression and distribution of AIM2 in the rat dental pulp tissues and cells in the presence or absence of inflammatory stimulation. Western blotting and real-time polymerase chain reaction were performed to determine whether there was a correlation between AIM2 expression levels and inflammation both in vivo and in vitro. RESULTS:In healthy dental pulp tissues and cells, AIM2 was only detected in the odontoblast layer. Stimulation significantly increased AIM2 expression in both the dental pulp tissues and cultured cells. The mRNA and protein levels of AIM2 were significantly up-regulated in response to inflammatory stimulation in a dose-dependent manner. Moreover, we also found that AIM2 expression correlated with interleukin-1 levels. These results reveal a direct relationship between the AIM2 inflammasome and pulpitis. CONCLUSIONS:Our study demonstrates that AIM2 is expressed in dental pulp tissues and mediates the inflammatory response during pulpitis. Therapeutic interventions aimed at reducing AIM2 expression may be beneficial in the treatment of pulpitis.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wang Y,Zhai S,Wang H,Jia Q,Jiang W,Zhang X,Zhang A,Liu J,Ni Ldoi
10.1016/j.joen.2013.07.003subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1390-4issue
11eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00598-0journal_volume
39pub_type
杂志文章abstract::Ten extracted human maxillary first molars were selected with a variety of root curvatures. The distofacial roots were resected and the mesiofacial canals were instrumented to a size #25 K file. Three techniques were used to introduce Ca(OH)2 paste into the instrumented canals. These techniques were evaluated for thei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)81220-3
更新日期:1992-08-01 00:00:00
abstract:INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.028
更新日期:2014-10-01 00:00:00
abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.024
更新日期:2017-09-01 00:00:00
abstract::Ocular complications after an inferior alveolar nerve anesthesia are rare. These complications, although temporary and benign, can be distressing to both the patient and the clinician. A 37-year-old male patient was administered an inferior alveolar nerve block for the root canal treatment of tooth #30. Immediately af...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2018.05.005
更新日期:2018-09-01 00:00:00
abstract::Biopsy reports of specimens from the apices of 1659 teeth physically present at the time the biopsy was performed were reviewed. The following information was recorded from each report: (a) source of specimen, (b) sex of patient, (c) age of patient, (d) location of tooth associated with lesion, and (e) diagnoses repor...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/s0099-2399(06)81679-1
更新日期:1990-05-01 00:00:00
abstract::The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolars were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleach...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200001000-00006
更新日期:2000-01-01 00:00:00
abstract:INTRODUCTION:This study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (MTA) mixed with aqueous and propylene glycol extracts of Arctium lappa L. and Casearia sylvestris Sw. METHODS:The setting-time test was performed according to American Society for Testin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.12.023
更新日期:2017-05-01 00:00:00
abstract:INTRODUCTION:Neuropeptides play an important role in inflammation and repair and have been implicated in mediating angiogenesis. Pulp fibroblasts express neuropeptide receptors, and the aim of this research was to investigate whether neuropeptides could regulate angiogenic growth factor expression in vitro METHODS:An ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.03.005
更新日期:2009-06-01 00:00:00
abstract::The aim of this study was to determine the effects of patient's age on the prevalence and depth of bacterial penetration inside dentinal tubules. Fifty-six single-rooted teeth were divided in two groups based on the patient's age: young (ages 18-25) and old (age >or=60). Teeth were instrumented and inoculated with a s...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.10.004
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.07.007
更新日期:2012-11-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to analyze and characterize root canal morphology of mandibular molars of the Brazilian population by using cone-beam computed tomography (CBCT). METHODS:Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.04.030
更新日期:2013-07-01 00:00:00
abstract:INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.016
更新日期:2011-11-01 00:00:00
abstract::The purpose of this study was to compare Schick CDR and Trophy RVGui direct digital radiography (DDR) systems for the ability to detect periapical lesions in human cadaver mandibles. Digital radiographs were exposed of teeth with normal periapical areas and of teeth with artificially prepared periapical lesions using ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000140571.23012.ff
更新日期:2005-04-01 00:00:00
abstract::Occurrence of apical transportation (AT) may complicate the root filling procedure and result in a compromised seal. In part I of this study, human mandibular premolars with single, curved (21 to 39 degrees), or straight canals were prepared by Lightspeed or a step-back hand filing technique. An AT index was determine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200004000-00003
更新日期:2000-04-01 00:00:00
abstract:INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.011
更新日期:2014-02-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the efficacy of passive ultrasonic irrigation (PUI) and the EndoVac (EV) System (Discus Dental, Culver City, CA) in hard tissue debris removal and its influence on the quality of the root canal filling with the aid of micro-computed tomographic scanner. METHODS:Twenty-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.05.001
更新日期:2015-10-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to evaluate the pH and calcium ion release of calcium hydroxide pastes associated with different substances. METHODS:Forty acrylic teeth with simulated root canals were divided into 4 groups according to the substance associated to the calcium hydroxide paste: chlorhexidine...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.05.009
更新日期:2009-09-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to determine the variations in root canal length (RCL) occurring during endodontic treatment stages (initial, preflared, and concluded) and correlate them with the accuracy of Root ZX II (RZX). METHODS:After coronal access, 26 mandibular molars had the apical foramen of the 52 me...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.07.022
更新日期:2016-11-01 00:00:00
abstract:INTRODUCTION:This review aimed to find the most effective oral premedication in reducing pain in adults after nonsurgical root canal therapy (NSRCT) using network meta-analysis. METHODS:The review protocol was registered in the PROSPERO database (CRD42017071899). A literature search was performed in the MEDLINE and EB...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.10.016
更新日期:2019-04-01 00:00:00
abstract:INTRODUCTION:This retrospective study aimed to analyze the effect of repairing endodontic access cavities with composite on the survival of single crowns and retainer restorations of fixed or removable dental prostheses. METHODS:Dental records of patients attending a university dental clinic were retrospectively scree...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.11.012
更新日期:2020-03-01 00:00:00
abstract:INTRODUCTION:The aims of this study were to examine the immunolocalization of protein phosphatase 1 (PP1) in developing mouse pulp tissue and to explore the role of PP1 in odontoblastic differentiation and in vitro angiogenesis in human dental pulp cells (HDPCs). METHODS:Immunolocalization of PP1 was assessed in devel...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.013
更新日期:2017-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root den...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.08.011
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:The success of endodontic regeneration lies in the appropriate combination of stem cells and bioactive materials. Several novel dental materials are available on the market in this regard. Hence, the current study aimed to evaluate the proliferation, differentiation, and osteogenic potential of human bone ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.11.016
更新日期:2018-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the volume of removed dentin, transportation, and centering ability of ProTaper Next (PTN) system with and without glide path preparation by using cone-beam computed tomography (CBCT) imaging. METHODS:Sixty mesiobuccal canals of mandibular first molars...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.09.001
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the shaping ability of 4 different nickel-titanium systems, considering their variation in alloy treatment, kinematics, and design, used to prepare canals with severe curvature using micro-computed tomographic analysis. METHODS:Thirty-two mesial roots of mandibular mol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.02.018
更新日期:2019-06-01 00:00:00
abstract::In the rat, perforations of maxillary molars were created and treated with either tricalcium phosphate (Synthograft) or Cavit. At four time intervals (1 day, 1 wk, 2 wk, and 1 month), four evaluative factors (inflammation, bone resorption, cementum and dentin resorption, and epithelial proliferation) were analyzed and...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(89)80171-2
更新日期:1989-09-01 00:00:00
abstract:INTRODUCTION:This retrospective cohort study aimed to observe the incidence of pulpitis and necrosis in teeth with cracks that were not endodontically treated. METHODS:One hundred eighty-four patients with 199 cracked teeth that were diagnosed with reversible pulpitis and were treated from January 2010 to December 201...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.02.003
更新日期:2019-05-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to investigate the effects of instrumentation and irrigation on the initial adherence of Enterococcus faecalis to root canal dentin and to explore initial microbial adhesion to root filling materials. METHODS:The following specimens were prepared: instrumented and uninstrumen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.04.006
更新日期:2019-07-01 00:00:00
abstract::Intracoronal bleaching of pulpless teeth with 30% hydrogen peroxide may result in external cervical root resorption. One of several suggested etiological factors associated with this complication is the pH of the bleaching materials. Because the available data on the pH values of bleaching materials were conflicting, ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81988-6
更新日期:1991-08-01 00:00:00
abstract:INTRODUCTION:Pulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via G...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.003
更新日期:2020-01-01 00:00:00