Abstract：
:Stem cell therapy is a potential strategy to regenerate the dentin-pulp complex, enabling the conservation and restoration of functional teeth. We assessed the efficacy and safety of pulp stem cell transplantation as a prelude before the initiation of clinical trials. Granulocyte-colony stimulating factor (G-CSF) induces subsets of dental pulp stem cells to form mobilized dental pulp stem cells (MDPSCs). Good manufacturing practice is a prerequisite for the isolation and expansion of MDPSCs that are enriched in stem cells, expressing a high level of trophic factors with properties of high proliferation, migration, and antiapoptotic effects and endowed with regenerative potential. The quality of clinical-grade MDPSCs was assured by the absence of abnormalities/aberrations in karyotype and the lack of tumor formation after transplantation in immunodeficient mice. Autologous transplantation of MDPSCs with G-CSF in pulpectomized teeth in dogs augmented the regeneration of pulp tissue. The combinatorial trophic effects of MDPSCs and G-CSF on cell migration, antiapoptosis, immunosuppression, and neurite outgrowth were also confirmed in vitro. Furthermore, MDPSCs from the aged donors were as potent as the young donors. It is noteworthy that there were no significant age-related changes in biological properties such as stability, regenerative potential, and expression of the senescence markers in MDPSCs. On the other hand, autologous transplantation of MDPSCs with G-CSF induced less regenerated pulp tissue in the aged dogs compared with the young dogs. In conclusion, the preclinical safety, feasibility, and efficacy of pulp regeneration by MDPSCs and G-CSF were established. Therefore, the standardization and establishment of regulatory guidelines for stem cell therapy in clinical endodontics is now a reality.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Nakashima M,Iohara Kdoi
10.1016/j.joen.2014.01.020subject
Has Abstractpub_date
2014-04-01 00:00:00pages
S26-32issue
4 Suppleissn
0099-2399issn
1878-3554pii
S0099-2399(14)00081-8journal_volume
40pub_type
杂志文章abstract：:This study was conducted to evaluate the influence of immediate versus delayed immersion time, and passive dye immersion versus centrifuged dye on apical leakage measurements. Eighty-four extracted human teeth with single straight canals were instrumented and divided into four experimental groups of 20 teeth each plus...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81364-6
更新日期：1993-07-01 00:00:00
abstract：INTRODUCTION:The retrieval of fractured instruments from root canals has been largely reported in the literature. However, a less complex retrieval method that causes minimum damage to the dental structures is needed. The aim of this article is to introduce a new concept of retrieval of fractured instruments from root ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.024
更新日期：2010-04-01 00:00:00
abstract：:The purpose of this study was to evaluate the efficacy of digital subtraction radiography using a direct digital imaging system in the follow-up study of endodontically treated teeth. The RVG-S was used as a direct digital imaging system. The intraimage variation of the original RVG-S image caused by dark current and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200208000-00006
更新日期：2002-08-01 00:00:00
abstract：INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.08.010
更新日期：2017-12-01 00:00:00
abstract：:The purpose of this study was to determine the incidence of endodontic services provided to participants in a longitudinal cohort study. The "Florida Dental Care Study" was a prospective cohort study using a representative baseline sample of 873 dentate adults. An in-person interview and clinical dental exam were cond...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200312000-00005
更新日期：2003-12-01 00:00:00
abstract：:Non-surgical endodontic treatment represents a cornerstone of contemporary endodontic care. Reflecting the central importance of this therapy, there is a high number of studies that address issues related to this treatment. Unfortunately, many of these studies are preclinical "bench top" studies with largely undefined...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2009.05.019
更新日期：2009-08-01 00:00:00
abstract：INTRODUCTION:Different single-file systems are available for endodontic treatment; however, comparative studies are scarce. Thus, the present study evaluated bacterial reduction promoted by 2 single-file systems: Wave One (Dentsply Maillefer, Ballaigues, Switzerland) and One Shape (Micromega, Besancon, France). METHOD...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.024
更新日期：2014-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare centering ability and apical canal transportation of K-files (KF), ProGlider (PG), and G-Files (GF) after glide path enlargement in curved canals by using micro-computed tomography. METHODS:Thirty canals each were randomly assigned to KF, GF, and PG. Teeth were sca...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.04.005
更新日期：2016-07-01 00:00:00
abstract：:Enterococcus faecalis, a pathogenic gram-positive bacterium, is closely related to refractory apical periodontitis. Because lipoteichoic acid (LTA) is considered a major virulence factor of gram-positive bacteria, in the present study, highly pure LTA from E. faecalis was prepared, and its ability to stimulate murine ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.05.005
更新日期：2008-08-01 00:00:00
abstract：INTRODUCTION:The objective of this study was to compare the outcomes of initial nonsurgical root canal therapy for different tooth types provided by both endodontists and other providers. METHODS:By using an insurance company database, 487,476 initial nonsurgical root canal therapy procedures were followed from the ti...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.02.008
更新日期：2016-05-01 00:00:00
abstract：:The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO prod...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.07.012
更新日期：2007-11-01 00:00:00
abstract：:This study evaluated the degree of conversion of one dual-cured resin cement when used to lute fiber posts with different translucencies. To measure the degree of conversion, polyvinylsiloxane molds were prepared to simulate root canals. The posts, Aestheti-Post or Light-Post, were cemented in these molds and, after p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.11.015
更新日期：2007-03-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS:Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.09.003
更新日期：2017-01-01 00:00:00
abstract：INTRODUCTION:Long-term predictability of restored endodontically treated teeth is important for the decision of tooth retention versus extraction and implant placement. The purpose of this study was to validate the hypothesis that preoperative factors can predict the long-term prognosis of molars requiring endodontic a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.10.005
更新日期：2011-01-01 00:00:00
abstract：:A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.11.004
更新日期：2016-04-01 00:00:00
abstract：:This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a sta...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80521-2
更新日期：1995-10-01 00:00:00
abstract：:This study investigated possible collateral C-fiber innervation between the pulps of rat molars by assessing neurogenic inflammation (NI) induced by the C-fiber excitant mustard oil (MO). MO was applied to the pulp of the left mandibular first molar in two groups of rats: group 1, guanethidine sympathectomized rats (t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80242-1
更新日期：1996-08-01 00:00:00
abstract：:Endodontic microsurgery has reduced the degree of treatment trauma compared with traditional apical surgery and further increased the success rate of natural teeth retention. However, when root apices of the mandibular posterior teeth are far from the buccal cortical bone surface or near to the inferior alveolar nerve...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.07.033
更新日期：2020-08-11 00:00:00
abstract：INTRODUCTION:Pulp revascularization may be considered a promising alternative for necrotic immature teeth. Many studies have accomplished passive decontamination associated with an antibiotic paste. To date, there is no report evaluating calcium hydroxide associated with 2% chlorhexidine gel for revascularization thera...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.10.005
更新日期：2013-03-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to verify the expression of the calcium-sensing receptor (CaSR) and its role in mineral trioxide aggregate (MTA)-induced odontoblastic differentiation and mineralization in human dental pulp cells (hDPCs). METHODS:The expression of CaSR in human dental pulp tissue and hDPCs w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.019
更新日期：2019-07-01 00:00:00
abstract：INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.015
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the prevalence and characteristics of postoperative pain after endodontic microsurgery and to identify potential predictors for severe pain. METHODS:One hundred seventy-three patients who underwent endodontic microsurgery at a private practice were included in the st...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.12.012
更新日期：2020-12-23 00:00:00
abstract：INTRODUCTION:A number of factors affect the dimensional stability of a material. These factors include curing conditions, material solubility, leaching, and time. The aim of this study was to evaluate the restrained dimensional change in the vertical direction as a function of the ambient conditions, fluid uptake, solu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.11.012
更新日期：2011-02-01 00:00:00
abstract：INTRODUCTION:The objective of this study was to investigate the expression of matrix metalloproteinases (MMPs) in apical periodontitis and during the periapical healing phase after root canal treatment. METHODS:Apical periodontitis was induced in dog teeth, and root canal treatment was performed in a single visit or b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.10.030
更新日期：2010-02-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the tooth structure theoretically required to be removed in the coronal and cervical regions to accomplish 3 different endodontic access preparations in a novel digital model. The deviation of the center of access cavity from the central fossa will also be measured...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.01.002
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:The aim of the present systematic review and meta-analysis was to evaluate the success and survival rate of endodontically treated cracked posterior teeth and to assess the preoperative factors that affect teeth survival. METHODS:The study protocol was registered on the PROSPERO international prospective ...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2020.01.006
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the kinematics of 2 reciprocating motors and compare it with manufacturers' declared values. METHODS:VDW Silver (VDW, Munich, Germany) and ATR Tecnika (Tecnika, Pistoia, Italy) were used in the study in 5 working modes: continuous rotation at 400 rpm on VDW Silver and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.12.008
更新日期：2014-07-01 00:00:00
abstract：INTRODUCTION:This study quantitatively evaluated the type and amount of image artifacts generated by different intracanal materials in birooted teeth scanned at different exposure parameters. METHODS:The sample consisted of 15 birooted premolars. Seven different intracanal material combinations were used in each tooth...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.11.007
更新日期：2019-02-01 00:00:00
abstract：INTRODUCTION:MicroRNAs (miRs) are a family of noncoding RNAs that regulate gene expression. They are ubiquitous among multicellular eukaryotes and are also encoded by some viruses. Upon infection, viral miRs (vmiRs) can potentially target gene expression in the host and alter the immune response. Although prior studies...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.006
更新日期：2017-01-01 00:00:00
abstract：:It has been shown that rotary nickel-titanium files cannot be used indefinitely. Researchers and clinicians have not been able to agree on how many times a file can be used before being discarded, except if a file has fractured or become visibly distorted. This study used ISO size 20 files of 0.04 taper in the curved ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200202000-00014
更新日期：2002-02-01 00:00:00
