Abstract：
INTRODUCTION:Alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. The aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (S1), after chemomechanical root canal preparation (S2), and after calcium hydroxide dressing (S3). METHODS:Bacteriologic samples were obtained from 43 primary infected root canals. Samples were inoculated into culture media at a pH of 9 and incubated anaerobically. The identities of bacterial isolates were determined by 16S ribosomal RNA sequencing. RESULTS:All S1 samples were culture positive, with 70% harboring bacteria tolerating a pH of 9. Gram-positive bacteria Pseudoramibacter alactolyticus and Streptococcus spp were the most frequently isolated strains with a prevalence of 54%. Of 13 culture-positive S2 samples, 8 isolates tolerated a pH of 9, namely Streptococcus sanguinis, Enterococcus faecalis, Enterobacter cancerogenus, Streptococcus oralis, and Fusobacterium nucleatum. Seven of these 8 isolates (88%) were correspondingly isolated at S1. All 3 culture-positive S3 samples tolerated a pH of 9, namely S. sanguinis and E. faecalis, which were also isolated in the corresponding S1 and S2 samples. CONCLUSIONS:We showed that the presence of alkaline-tolerant Streptococcus and Enterococcus spp in primary infected root canals could lead to their persistence during and after root canal treatment and could pose a challenge to current treatment efficacy.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Lew HP,Quah SY,Lui JN,Bergenholtz G,Hoon Yu VS,Tan KSdoi
10.1016/j.joen.2014.12.003subject
Has Abstractpub_date
2015-04-01 00:00:00pages
451-6issue
4eissn
0099-2399issn
1878-3554pii
S0099-2399(14)01213-8journal_volume
41pub_type
杂志文章abstract：INTRODUCTION:The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation. METHODS:One hundred twelve dentin bloc...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.07.018
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to verify the expression of the calcium-sensing receptor (CaSR) and its role in mineral trioxide aggregate (MTA)-induced odontoblastic differentiation and mineralization in human dental pulp cells (hDPCs). METHODS:The expression of CaSR in human dental pulp tissue and hDPCs w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.019
更新日期：2019-07-01 00:00:00
abstract：:Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.023
更新日期：2006-10-01 00:00:00
abstract：INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.09.020
更新日期：2017-01-01 00:00:00
abstract：INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：:This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.016
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:Alkaline phosphatase (ALP) plays an important role in inducing mineralization events in the dental pulp. This study investigated and compared the ALP levels in healthy and inflamed pulp in young and old human pulp. METHODS:Tissue samples were collected from young (<30 years) and old (>60 years) donors. In...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.10.003
更新日期：2016-01-01 00:00:00
abstract：INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.10.019
更新日期：2021-02-01 00:00:00
abstract：:Previous dye leakage studies have shown that mineral trioxide aggregate leaks significantly less than other commonly used root-end filling materials. This study determined the time needed for Staphylococcus epidermidis to penetrate a 3-mm thickness of amalgam, Super-EBA, Intermediate Restorative Material (IRM), or min...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80433-4
更新日期：1995-03-01 00:00:00
abstract：:The cleaning capacity of manual and rotary instrumentation techniques in mesial-distal flattened canals was studied by morphometric analysis. Twenty human mandibular incisors were divided into two groups of 10 teeth each: group 1, crown-down technique with rotary instrumentation using ProFile .04; group 2, crown-down ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200205000-00004
更新日期：2002-05-01 00:00:00
abstract：INTRODUCTION:Idiopathic bone cavity (IBC) is an uncommon bone lesion that usually affects youngsters as an unilocular radiolucency with predilection for the posterior mandible. Because the lesion is frequently located in proximity to the adjacent teeth, chronic apical periodontitis is commonly included as a differentia...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.11.004
更新日期：2021-02-01 00:00:00
abstract：INTRODUCTION:Previous studies have suggested that masticatory forces are associated with cracked teeth, but it is not clear which specific poor oral masticatory habits may be more likely to cause cracks. The aim of this study was to quantitatively assess risk factors for cracked teeth among poor oral masticatory habits...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.01.007
更新日期：2017-06-01 00:00:00
abstract：:The cyclic fatigue resistance of three types of nickel-titanium rotary files was compared in a model using reciprocating axial movement. The influence of file size and taper was also investigated and fracture patterns were examined under SEM. The 10 experimental groups consisted of ProFiles, K3s, and RaCe files, size ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.013
更新日期：2006-01-01 00:00:00
abstract：:A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.07.015
更新日期：2006-12-01 00:00:00
abstract：INTRODUCTION:This in vitro study compared the cyclic fatigue resistance of ProFile Vortex rotary instruments (DENTSPLY Tulsa Dental, Tulsa, OK) made of two different raw materials: M-Wire and regular superelastic wire (SE-wire) at two different rotational speeds. Two rotary-based Profile Vortex instruments with taper 0...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.02.015
更新日期：2010-07-01 00:00:00
abstract：:The occurrence of Enterococcus faecalis in root canals of previously root filled teeth with apical periodontitis requiring retreatment was studied in Lithuanian patients. Twenty-five asymptomatic teeth were included in the study. Avoiding contamination microbiological samples were taken from the canals before and afte...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200010000-00004
更新日期：2000-10-01 00:00:00
abstract：:Terrein is a bioactive fungal metabolite whose anti-inflammatory properties are virtually unknown. The purpose of this study was to determine the effects of terrein on lipopolysaccharide (LPS)-induced expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human denta...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.01.015
更新日期：2008-04-01 00:00:00
abstract：INTRODUCTION:Difficult handling, long setting time, and potential discoloration are important drawbacks of white mineral trioxide aggregate (WMTA). The development of Biodentine, a recently developed calcium silicate-based material (CSM), has overcome some of these shortcomings; however, there are no available data on ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.021
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:Teeth undergoing initial endodontic therapy have a very high survival rate. Some teeth that continue to show signs of pathosis after the initial therapy will require nonsurgical (orthograde) retreatment. Outcome assessment of endodontic retreatment is crucial for appropriate case selection and treatment pl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.02.009
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in regulating tricalcium silicate (C3S)-driven proliferation and biomineralization of human dental pulp cells (hDPCs) in vitro. METHODS:Human DPCs were cultured in C3S-containing medium and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.03.002
更新日期：2013-08-01 00:00:00
abstract：:The anatomy of third molars has been described as unpredictable. However restorative, prosthetic, and orthodontic considerations often require endodontic treatment of third molars in order for them to be retained as functional components of the dental arch. The purpose of this study was to investigate and characterize...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200011000-00011
更新日期：2000-11-01 00:00:00
abstract：:A case report is presented describing the treatment for endemic dental fluorosis. Six maxillary anterior teeth were treated, three with an acid-bleach combination technique and three with an acid technique. No clinical differences was noted in treatment time or esthetic results. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81703-6
更新日期：1991-07-01 00:00:00
abstract：:The effects of parathyroid hormone (PTH), 1,25-dihydroxyvitamin D3, and prostaglandin E2 (PGE2) on alkaline phosphatase activity on cultured dental pulp and gingiva cells of bovine calf were compared. In pulp cells, PTH, 1,25-dihydroxyvitamin D3, and PGE2 significantly increased alkaline phosphatase activity, but no i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)82009-1
更新日期：1991-04-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.07.002
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:Electronic working length measurement during root canal treatment in teeth with enlarged apical foramina is a challenge. The aims of this in vitro study were (1) to assess the influence of foramen widening on the accuracy of 5 different electronic foramen locators (EFLs) and (2) to compare the accuracy of ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.09.032
更新日期：2014-01-01 00:00:00
abstract：INTRODUCTION:The aims of this study were to examine the immunolocalization of protein phosphatase 1 (PP1) in developing mouse pulp tissue and to explore the role of PP1 in odontoblastic differentiation and in vitro angiogenesis in human dental pulp cells (HDPCs). METHODS:Immunolocalization of PP1 was assessed in devel...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.013
更新日期：2017-03-01 00:00:00
abstract：:The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.021
更新日期：2007-08-01 00:00:00
abstract：INTRODUCTION:This review aimed to compare the kinematic effect of nickel-titanium instruments with reciprocating and continuous rotation motion for cyclic fatigue resistance, shaping ability, apical debris extrusion, and dentinal defects or cracks. METHOD:Articles were selected for inclusion in this review if they ful...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2016.04.002
更新日期：2016-07-01 00:00:00
abstract：INTRODUCTION:Pain present 6 months after root canal treatment (RCT) may be of odontogenic or nonodontogenic origin. This is important because treatments and prognoses are different; therefore, the aim of this study was to provide specific diagnoses of patients reporting pain 6 months after receiving initial orthograde ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.12.012
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of pH that is due to setting reaction of Biodentine, glass ionomer cement (GIC), and intermediate restorative material (IRM) on transforming growth factor-β1 (TGF-β1) release and on the fibrin architecture of platelet-rich fibrin (PRF). METHODS:PRF w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.02.009
更新日期：2016-05-01 00:00:00
