Abstract：
:The purpose of this study was to investigate whether the inflammation of rat dental pulp induces the muscarinic acetylcholine receptor (mAChR) constitutive receptor activity. Pulpitis was induced with bacterial lipolysaccharide in rat incisors dental pulp. Saturation assay with [(3)H]-quinuclidinyl benzilate ([(3)H] QNB), competitive binding with different mAChR antagonist subtypes, and nitric oxide synthase (NOS) activity were performed. A drastic change in expression and response to mAChR subtypes was observed in pulpitis. Inflamed pulp expressed high number of M(3) mAChR of high affinity, whereas the M(1) mAChR is the main subtype displayed in normal pulp. Consistent with the identification of the affinity constant (Ki) of M(3) and Ki of M(1) in both pulpitis and in normal pulps are the differences in the subtype functionality of these cells. In pulpitis, pilocarpine (1 × 10(-11) mol/L to 5 × 10(-9) mol/L) exerted an inhibitory action on NOS activity that was blocked by J 104129 fumarate (highest selective affinity to M(3) mAChR). In normal pulps, pilocarpine (1 × 10(-11) mol/L to 5 × 10(-9) mol/L) has no effect. NOS basal activity was 5.9 times as high in pulpitis as in the normal pulp as a result of the activation of inducible NOS. The irreversible pulpitis could induce a mAChR alteration, increasing the high-affinity receptor density and transduction-coupling efficiency of inducible NOS activity, leading to a spontaneously active conformation of the receptor. Pilocarpine acting as an inverse agonist might be useful therapeutically to prevent necrosis and subsequent loss of dental pulp.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Sterin-Borda L,Orman B,De Couto Pita A,Borda Edoi
10.1016/j.joen.2010.11.021subject
Has Abstractpub_date
2011-02-01 00:00:00pages
185-90issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(10)00929-5journal_volume
37pub_type
杂志文章abstract：INTRODUCTION:This study compared the accuracy of cone beam computed tomography (CBCT) scans made by five different systems in detecting vertical root fractures (VRFs). It also assessed the influence of the presence of root canal filling (RCF), CBCT slice orientation selection, and the type of tooth (premolar/molar) on ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.09.013
更新日期：2010-01-01 00:00:00
abstract：INTRODUCTION:The health of human teeth depends on the integrity of the hard tissue and the activity of the pulp and periodontal tissues, which are responsible for nutritional supply. Without the nourishing of the pulp tissue, the possibility of tooth fracture can increase. In immature permanent teeth, root development ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.10.002
更新日期：2013-02-01 00:00:00
abstract：INTRODUCTION:An accurate differential diagnosis of apical periodontitis (AP) and nasopalatine duct cyst (NPDC) should be established to define the best treatment for endodontically treated maxillary anterior teeth with apical periodontitis. Three-dimensional cone beam computed tomography (CBCT) images help to plan trea...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.11.022
更新日期：2011-03-01 00:00:00
abstract：INTRODUCTION:This retrospective study assessed the effects of microsurgical treatment of post-treatment endodontic disease using SuperEBA (Bosworth, Skokie, IL) as the root-end filling material and evaluated the potential prognostic factors in relation to outcome. METHODS:Data were collected from patients diagnosed wi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.11.001
更新日期：2014-03-01 00:00:00
abstract：:The finite element method was used to compare stresses along the inner canal wall in four two-dimensional models of an average maxillary central incisor. The four models evaluated were an intact incisor, an endodontically treated incisor, an endodontically treated crown-restored incisor, and a cylindrical post and cro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81210-0
更新日期：1992-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare, clinically and radiographically, the effects of platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA) as pulpotomy agents in permanent teeth with incomplete root development. METHODS:A total number of 70 children requiring pulpotomy in 70 pe...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2014.01.009
更新日期：2014-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the push-out bond strengths of different obturating materials. METHODS:Forty single-rooted human extracted teeth were used in this study. The teeth were instrumented and irrigated by using 5.25% NaOCl, 15% ethylenediaminetetraacetic acid, and sterile water. The te...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.10.026
更新日期：2010-02-01 00:00:00
abstract：INTRODUCTION:Various factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.011
更新日期：2019-06-01 00:00:00
abstract：:The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO prod...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.07.012
更新日期：2007-11-01 00:00:00
abstract：INTRODUCTION:Calcium hydroxide has been used as a traditional pulpotomy agent for a long time but has some disadvantages. iRoot BP Plus (Innovative Bioceramix Inc, Vancouver, Canada) is a newly developed, ready-to-use calcium silicate-based bioactive ceramic with excellent bioactivity and sealing ability. However, whet...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.12.010
更新日期：2020-03-01 00:00:00
abstract：:The aim of this study was to evaluate the time required by four different root canal medications coupled with the temporary filling material Cavit (ESPE, Seefeld, Germany) to prevent penetration of bacteria into the root canal. There were 145 roots prepared in a standardized manner. Four groups with 15 samples each we...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.023
更新日期：2006-02-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare canal transportation and the centering ability of Reciproc (VDW, Munich, Germany), WaveOne (Dentsply Maillefer, Ballaigues, Switzerland), and EdgeFile (EdgeEndo, Albuquerque, NM) rotary systems using cone-beam computed tomographic imaging. METHODS:Ninety mesiobucca...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.011
更新日期：2018-12-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：INTRODUCTION:Recently, it was shown that the biomineralization process promoted by the interaction of mineral trioxide aggregate (MTA) with dentin in phosphate-buffered saline (PBS) positively influenced the push-out bond strength of the cement. This study investigated if the use of a PBS intracanal dressing promotes t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.014
更新日期：2010-10-01 00:00:00
abstract：:It is thought that externally applied bleaching agents may penetrate into the pulp chamber. This study was conducted to evaluate the diffusion of peroxide bleaching agents into the pulp chamber of teeth restored with various restorative materials. Sixty-five human extracted anterior maxillary teeth were separated into...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200002000-00008
更新日期：2000-02-01 00:00:00
abstract：:A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200206000-00001
更新日期：2002-06-01 00:00:00
abstract：INTRODUCTION:The aim of the present study was to investigate the impact of ambient temperature on the life span of nickel-titanium (NiTi) files. METHODS:Cyclic fatigue testing of NiTi instruments was performed in a stainless steel artificial canal. During the experiment, 4 different temperatures (0°C, 20°C, 35°C, and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.06.006
更新日期：2018-10-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of citric acid and EDTA solutions on the microhardness and the roughness of human root canal dentin. Forty-five human teeth sectioned longitudinally were used. Specimens were randomly divided into three groups of 30 teeth each and were treated as follows: (a) one mo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000136212.53475.ad
更新日期：2005-02-01 00:00:00
abstract：:The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200406000-00010
更新日期：2004-06-01 00:00:00
abstract：:A previous study showed that the ferret canine is an appropriate model for inducing periapical lesions. Ferret canines were used in this study to study changes in periapical lesions after root canal treatment (RCT). After periapical pathosis was induced in the maxillary and mandibular canines of six ferrets, RCT was p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80506-6
更新日期：1993-03-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.034
更新日期：2017-03-01 00:00:00
abstract：:Three randomly assigned groups of single-canaled extracted teeth obturated with gutta-percha were retreated using controlled application of one of three organic solvents: chloroform, xylene, or halothane. Two additional groups of teeth served as positive and negative controls. Residual volume of solvent expressed thro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80098-8
更新日期：1998-04-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to examine the effect of mineral trioxide aggregate (MTA) on pluripotent-mesenchymal cell differentiation. METHODS:The pluripotent-mesenchymal cell line C2C12 was cultured in a 5% serum medium to induce cell differentiation with or without MTA. The differentiation to myoblast...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.10.006
更新日期：2013-01-01 00:00:00
abstract：:This study attempts to evaluate the oral manifestations of and the limited available dental pulp information on diabetes mellitus, a common metabolic disorder of carbohydrate and lipid metabolism affecting over 16 million Americans. Diabetics are particularly prone to bacterial or opportunistic infections. This vulner...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/00004770-200306000-00001
更新日期：2003-06-01 00:00:00
abstract：:A technique utilizing fiberoptics to transilluminate a beveled root surface during periradicular surgery is described. This procedure when integrated with other standard endodontic surgical aids may help diagnose apical variations and vertical root fractures. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80301-8
更新日期：1994-08-01 00:00:00
abstract：INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.04.028
更新日期：2010-08-01 00:00:00
abstract：:This report presents a rare case of odontogenic cutaneous sinus tract caused by a vertical root fracture. The root-fractured molar was probably caused by an accidental blow by a cow 3 years ago. Despite all treatments by physicians and surgeons for nearly 1 year, the sinus tract recurred repeatedly. After diagnosis an...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/s0099-2399(06)81130-1
更新日期：1997-09-01 00:00:00
abstract：:There have been major recent reorganizations among bacterial taxa as a result of phylogenetic taxonomic approaches. As a consequence, old species have been renamed and novel species have been proposed. The introduction of molecular technology for microbial identification has also allowed the detection of microbial tax...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/00004770-200310000-00001
更新日期：2003-10-01 00:00:00
abstract：INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：:This study investigated possible collateral C-fiber innervation between the pulps of rat molars by assessing neurogenic inflammation (NI) induced by the C-fiber excitant mustard oil (MO). MO was applied to the pulp of the left mandibular first molar in two groups of rats: group 1, guanethidine sympathectomized rats (t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80242-1
更新日期：1996-08-01 00:00:00
