Abstract：
INTRODUCTION:Real-time assessment of the microbial status of the root canal system would be useful in clinical endodontic practice for determining endpoints of biomechanical treatment. This laboratory study used an existing laser fluorescence device, the DIAGNOdent (KaVo, Biberach, Germany), in a proof-of-concept study. METHODS:Visible laser red light (wavelength 655 nm) was used to elicit fluorescence emissions in the near-infrared range from infected and uninfected root canals. A prototype sapphire tip designed for periodontal assessment was used to analyze the pulp chamber and coronal third of the root canal system in extracted teeth. The fluorescence properties of bacterial cultures, monospecies biofilms in root canals, pulpal soft tissues, and sound dentin were also evaluated, together with 50 extracted teeth with known endodontic pathology. RESULTS:Sound dentin and healthy pulpal soft tissue gave an average fluorescence reading of 5 (on a scale of 100), whereas biofilms of Enterococcus faecalis and Streptococcus mutans established in root canals showed a progressive increase in fluorescence over time. Fluorescence readings reduced to the "healthy" threshold reading of 5 when root canals were endodontically treated, and the experimentally created bacterial biofilms were removed completely. High fluorescence readings were recorded in the root canals and pulp chambers of extracted teeth with radiographic evidence of periapical pathology and scanning electron microscopy evidence of bacterial infection. CONCLUSIONS:The use of the DIAGNOdent fluorescence approach for the assessment of the status of the pulp chamber and root canal system holds promise for clinical application; once more, flexible tips can be developed for gaining greater penetration into middle and apical thirds of the root canal.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Sainsbury AL,Bird PS,Walsh LJdoi
10.1016/j.joen.2009.07.006subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1404-7issue
10eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00615-3journal_volume
35pub_type
杂志文章abstract：:It is a general belief that large cyst-like periapical lesions and apical true cysts caused by root canal infection are less likely to heal after nonsurgical root canal therapy. Nevertheless, there is no direct evidence to support this assumption. A large cyst-like periapical lesion or an apical true cyst is formed wi...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2009.02.012
更新日期：2009-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS:Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.09.003
更新日期：2017-01-01 00:00:00
abstract：:The cross-sectional configuration of seven files at coronal and apical levels were compared with diagrams presented by manufacturers. The presence of cutting edges was also examined. Ground-twisted (K-file, K-Reamer, and K-flex) and machined files (Flex-R, Unifile, S-file, and Hedstrom) were included in #10, #25, and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81111-8
更新日期：1995-12-01 00:00:00
abstract：:This study compared the microshear bond strength of three resin-based sealers to root dentin and assessed whether sealer cements behave differently in thin and thick films. Extracted maxillary premolars were sectioned buccolingually, and 45 root halves were randomly allocated for microshear bond testing with the three...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.10.009
更新日期：2009-01-01 00:00:00
abstract：:Strippings are problems that are frequent on thin and concave roots. Treatment and prognosis differ from that of a lateral root perforation because of the size, oval shape, and thin edges of the striping. We propose a two-step technique: an endodontic phase in which the root canal system is sealed with gutta-percha ov...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80069-0
更新日期：1996-12-01 00:00:00
abstract：INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.05.035
更新日期：2011-09-01 00:00:00
abstract：INTRODUCTION:In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.07.003
更新日期：2013-11-01 00:00:00
abstract：:The purpose of this study was to compare intracanal bacterial reduction on teeth instrumented with 0.04 tapered nickel-titanium (NiTi) rotary instrumentation to bacterial reduction when using a stainless-steel K-file step-back technique using sterile saline irrigation. Forty-eight patients with apical periodontitis we...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/S0099-2399(98)80170-2
更新日期：1998-11-01 00:00:00
abstract：INTRODUCTION:The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. T...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.016
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the extent of disinfection mediated by 2 concentrations of sodium hypochlorite for bacteria-laden dentin wall of a root canal. METHODS:A dual-species film, Enterococcus faecalis and Porphyromonas gingivalis, was cultivated on root canal dentin for 7 days. The canal w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.09.045
更新日期：2014-06-01 00:00:00
abstract：INTRODUCTION:Dentin regeneration is one of the main goals of vital pulp treatment in which the biological properties of dental pulp cells (DPCs) need to be considered. In our previous study, we showed that EDTA could enhance the stromal cell-derived factor 1 alpha-induced migration of DPCs. The purpose of this study wa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.12.003
更新日期：2020-12-20 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the bond strength of different adhesive sealers on Resilon and gutta-percha. METHODS:Pellets of gutta-percha and Resilon were embedded into test tubes. Small eyelets were attached to those surfaces with a 0.5-mm film of different root canal sealers. Real Seal, Real Se...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.01.004
更新日期：2010-05-01 00:00:00
abstract：:The bond strength of ActiV GP root canal filling system and gutta-percha/AH plus sealer when used after final rinse with different irrigation protocols was evaluated in this study. Forty roots were randomly divided into four groups (n = 10) according to the final irrigation regimen: group 1, 5 mL 17% EDTA; group 2, 5 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:The retrieval of fractured instruments from root canals has been largely reported in the literature. However, a less complex retrieval method that causes minimum damage to the dental structures is needed. The aim of this article is to introduce a new concept of retrieval of fractured instruments from root ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.024
更新日期：2010-04-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the kinematics of 2 reciprocating motors and compare it with manufacturers' declared values. METHODS:VDW Silver (VDW, Munich, Germany) and ATR Tecnika (Tecnika, Pistoia, Italy) were used in the study in 5 working modes: continuous rotation at 400 rpm on VDW Silver and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.12.008
更新日期：2014-07-01 00:00:00
abstract：:Ultrasonic root-end preparation techniques have recently been introduced and revolutionized the field of endodontic surgery. The purpose of this study was to compare quantitatively the quality of root-end preparation techniques prepared by a specially designed ultrasonic retrotip with those prepared in a traditional m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80152-0
更新日期：1998-10-01 00:00:00
abstract：:Use of resin-based restorative materials recently has become widely accepted for treatment of endodontically treated teeth. However, some solutions routinely used during endodontic treatment procedures may have an effect on bond strengths of adhesive materials to root canal dentin. The purpose of this in vitro study w...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200402000-00013
更新日期：2004-02-01 00:00:00
abstract：:This study evaluated the effect of three post surface treatments on quartz-fiber post (a) retention and (b) morphology. A control group was not treated. Different treatments were performed for the other posts: silanization; etching by hydrofluoric acid 9.5%; sandblasting with 50 microm Al2O3. Two specimens of each gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.10.015
更新日期：2007-03-01 00:00:00
abstract：:A retrograde root-end cover with a special resin composite (Retroplast) combined with the dentin bonding agent Gluma (Bayer AG) has been used since 1984 by the authors. Its content of silver, added to promote radiopacity, has been found to lower the working time and storage stability of the composite and might cause d...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章
doi：10.1016/S0099-2399(96)80082-3
更新日期：1996-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of various degrees of cyclic fatigue on torsional failure and torsional preloading on the cyclic fatigue life of heat-treated K3XF nickel-titanium (NiTi) instruments (SybronEndo, Orange, CA). METHODS:The mean number of cycles until failure (Nf) of K3XF ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.10.008
更新日期：2015-04-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.11.007
更新日期：2008-05-01 00:00:00
abstract：:The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200012000-00011
更新日期：2000-12-01 00:00:00
abstract：:Enterococcus faecalis, a pathogenic gram-positive bacterium, is closely related to refractory apical periodontitis. Because lipoteichoic acid (LTA) is considered a major virulence factor of gram-positive bacteria, in the present study, highly pure LTA from E. faecalis was prepared, and its ability to stimulate murine ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.05.005
更新日期：2008-08-01 00:00:00
abstract：:Two electronic apex locators based on the gradient (Apit) and absolute (Odontometer) impedance principles were evaluated. Distances of file tips to apical foramina were determined in vivo with the electronic apex locators and subsequently verified after extraction. Mean differences between file tips and apical foramin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80145-7
更新日期：1996-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to determine factors and clinical situations that influence an endodontist's decision to use guided tissue regeneration (GTR) techniques during endodontic root-end surgery. METHODS:An invitation to participate in a web-based survey was e-mailed to 3,750 members of the America...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.017
更新日期：2011-11-01 00:00:00
abstract：:Various classes of immunoglobulins have been found in human periapical lesions. The specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. The purpose of this study was to test the specificity of antibodies present in human periapical lesions. Hu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81923-0
更新日期：1991-05-01 00:00:00
abstract：:This report examines three clinical cases of root-filled teeth with primary endodontic lesions. During endodontic surgery, an area of incomplete root bifurcation beginning in the coronal or middle third of the root surface and terminating at or near the apical foramen were observed. The radicular grooves of two teeth ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200005000-00012
更新日期：2000-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effect of calcium hydroxide (CH) and antibiotic pastes, including a mixture of metronidazole and ciprofloxacin, with and without minocycline or cefaclor, on the dislocation resistance of mineral trioxide aggregate (MTA) to root dentin. METHODS:Eighty single-rooted...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.018
更新日期：2014-12-01 00:00:00
abstract：:Etch-and-rinse adhesives can cause vasorelaxation via mechanisms occurring in the endothelium and smooth muscle, including the release of nitric oxide (NO). This effect might promote or aggravate bleeding if such adhesives are placed inadvertently on iatrogenic pulp microexposures. The present study assessed the vasoa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.07.019
更新日期：2008-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare the centering ability and canal transportation of the ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland) and Self-adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) systems in long oval root canals using cone-beam computed tomography imag...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.07.007
更新日期：2019-10-01 00:00:00
