Abstract：
:Enterococcus faecalis, a pathogenic gram-positive bacterium, is closely related to refractory apical periodontitis. Because lipoteichoic acid (LTA) is considered a major virulence factor of gram-positive bacteria, in the present study, highly pure LTA from E. faecalis was prepared, and its ability to stimulate murine macrophages was investigated in comparison with those of the killed whole cells. Upon exposure to E. faecalis LTA, RAW 264.7 (a murine macrophage cell line) produced a significantly (p < 0.05) high level of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in a concentration-dependent manner. It is to note that the LTA was able to stimulate Toll-like receptor 2 (TLR2) but not TLR4. Concomitantly, LTA enhanced the DNA-binding activity of a transcription factor, nuclear factor-kappa B (NF-kappaB), which plays an important role in the transcriptional activation of genes encoding inflammatory mediators. In contrast, heat-killed E. faecalis stimulated both TLR2 and TLR4, whereas the killed E. faecalis whole cells induced significant (p < 0.05) levels of TNF-alpha and NO in RAW 264.7 cells as their LTA did. These results suggest that LTA partially contributes to E. faecalis-induced inflammatory responses.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Baik JE,Ryu YH,Han JY,Im J,Kum KY,Yun CH,Lee K,Han SHdoi
10.1016/j.joen.2008.05.005subject
Has Abstractpub_date
2008-08-01 00:00:00pages
975-82issue
8eissn
0099-2399issn
1878-3554pii
S0099-2399(08)00408-1journal_volume
34pub_type
杂志文章abstract：INTRODUCTION:The aim of this study was to compare the shaping ability of 4 different nickel-titanium systems, considering their variation in alloy treatment, kinematics, and design, used to prepare canals with severe curvature using micro-computed tomographic analysis. METHODS:Thirty-two mesial roots of mandibular mol...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.02.018
更新日期：2019-06-01 00:00:00
abstract：:Cyanoacrylate is an extremely strong bonding agent which has many applications in medicine. It is also effective in obtaining a leakproof seal of rubber dam around a structurally compromised tooth requiring endodontic therapy. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81356-7
更新日期：1992-10-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) has been used successfully for perforation repair, vital pulpotomies, and direct pulp capping. However, little is known about the interactions between MTA and glass ionomer cement (GIC) in final restorations. In this study, 2 null hypotheses were tested: (1) GIC placement t...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2012.04.013
更新日期：2012-08-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) is a potent mitogenic and angiogenic factor involved in the development of many tissues including the teeth. We previously reported that dental pulp cells express the HGF receptor c-met and that HGF can enhance cell proliferation and differentiation. The purpose of this study was to dete...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.031
更新日期：2009-03-01 00:00:00
abstract：:Dentinal chips and different biocompatible materials have been used in the past as apical plugs to enhance healing and simplify obturation. The purpose of this study was to evaluate bone reactions to implants of hydroxyapatite, collagen, and calcium hydroxide, alone or in different combinations. The materials were imp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(07)80175-0
更新日期：1990-10-01 00:00:00
abstract：:Three randomly assigned groups of single-canaled extracted teeth obturated with gutta-percha were retreated using controlled application of one of three organic solvents: chloroform, xylene, or halothane. Two additional groups of teeth served as positive and negative controls. Residual volume of solvent expressed thro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80098-8
更新日期：1998-04-01 00:00:00
abstract：:Coconut water is biologically pure and sterile, with a rich presence of amino acids, proteins, vitamins, and minerals. The purpose of this study was to use a collagenase-dispase assay to investigate the potential of a new storage medium, coconut water, in comparison with propolis, Hank's balanced salt solution (HBSS),...
journal_title：Journal of endodontics
pub_type： 杂志文章,收录出版
doi：10.1016/j.joen.2008.01.018
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:Teeth undergoing initial endodontic therapy have a very high survival rate. Some teeth that continue to show signs of pathosis after the initial therapy will require nonsurgical (orthograde) retreatment. Outcome assessment of endodontic retreatment is crucial for appropriate case selection and treatment pl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.02.009
更新日期：2010-05-01 00:00:00
abstract：:No information could be found in the dental literature on the hardness or strength of the materials in root canal instruments. Hardness testing is a convenient method for examining these products, and strength properties can be deduced from available strength-hardness data. The Vickers hardness of cross-sections of st...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80274-3
更新日期：1996-02-01 00:00:00
abstract：:An in vitro, flow-through optical system was designed to measure hemoglobin (Hb) concentrations in the pulp space. The system included light-emitting diodes and a silicon photodetector positioned on opposing surfaces of human teeth. A syringe pump allowed a controlled flow of blood through the pulp chamber. The Hb con...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80230-5
更新日期：1996-01-01 00:00:00
abstract：:The purpose of this in vitro study was to compare dye leakage between canals backfilled in a single increment and canals backfilled in multiple increments using the Obtura II system with two different sealers. Sixty extracted single canal teeth were decoronated, cleaned, and shaped. After master cone fit and placement...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80320-3
更新日期：1999-09-01 00:00:00
abstract：:In the present study, we used Fos expression as an index of nociceptive input to the spinal trigeminal nucleus after exposure of the coronal pulp tissue of maxillary right first molars and examined the effects of pretreatment with an opioid, a nonsteroidal anti-inflammatory drug or a local anesthetic before pulp expos...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000153844.61709.64
更新日期：2005-08-01 00:00:00
abstract：:Cytotoxicity was compared between new root canal sealers (New A, New B, and New B-2) and conventional root canal sealers (AH26, Diaket, Canals, Tubi-Seal, and Sealapex). Dental pulp cells of rats, obtained through primary cell culture, were used. In the experiments with the freshly prepared sealers, the incorporation ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80109-8
更新日期：1989-02-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to investigate the effects of instrumentation and irrigation on the initial adherence of Enterococcus faecalis to root canal dentin and to explore initial microbial adhesion to root filling materials. METHODS:The following specimens were prepared: instrumented and uninstrumen...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.04.006
更新日期：2019-07-01 00:00:00
abstract：:In the human dental pulp of five healthy premolars, we detected the presence of vesicles in the cytoplasm of macrophage-like cells. There were, in general, two types of vesicles, with regard to their size and content. The first was of smaller size (35 to 55 nm), and they were electron optically empty. The second type ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80176-3
更新日期：1998-03-01 00:00:00
abstract：INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.011
更新日期：2014-02-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the effects of pretreatment of gel chlorhexidine and ethanol on the bond strength and durability of the adhesion of the fiber post relined with resin composite to the root dentin. METHODS:Sixty bovine incisor roots were divided into four groups: irrigation with phy...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.01.007
更新日期：2011-05-01 00:00:00
abstract：:With all the potential for endodontic success, the fact remains clinicians are confronted with posttreatment endodontic disease. When the elected treatment plan is endodontic nonsurgical retreatment, then the goal is to access the pulp chamber and remove materials from the root canal space and if present, address defi...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/01.don.0000145033.15701.2d
更新日期：2004-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to investigate if nickel-titanium instruments with similar designs manufactured by different thermal treatments would exhibit significantly different in vitro behavior. METHODS:Thirty-six instruments each of ProTaper Universal (PTU F1; Dentsply Maillefer, Ballaigues, Switzerl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.06.005
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:Complement activation is considered a major mechanism in innate immunity. Although it is mainly involved in initiating inflammation, recent data reported its involvement in other processes such as tissue regeneration. In the dental pulp, complement C5a fragment has been shown to be involved in the recruitm...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.06.011
更新日期：2016-09-01 00:00:00
abstract：:This study attempts to evaluate the oral manifestations of and the limited available dental pulp information on diabetes mellitus, a common metabolic disorder of carbohydrate and lipid metabolism affecting over 16 million Americans. Diabetics are particularly prone to bacterial or opportunistic infections. This vulner...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/00004770-200306000-00001
更新日期：2003-06-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) satisfies most of the ideal properties of a surgical root-end filling and perforation repair material. It has been found to be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable and promotes cementogenesis. The major...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：:The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200406000-00010
更新日期：2004-06-01 00:00:00
abstract：:Fusion and gemination are developmental anomalies of the dental hard tissues that might require endodontic and surgical treatment for functional, orthodontic, or esthetic reasons. In this article, we report a rare case of successful endodontic management of unilateral fused mandibular second molar with a paramolar. Th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.06.021
更新日期：2007-10-01 00:00:00
abstract：:The purpose of this study was to compare ultrasonic and high-speed-bur root-end preparations. Seventy-six roots from 29 bilaterally matched pairs of human teeth in cadavers were used in this study. In group 1 ultrasonic preparations were made in 38 roots and filled with amalgam. In group 2 high-speed bur preparations ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(97)80301-9
更新日期：1997-07-01 00:00:00
abstract：:Apical periodontitis and its concomitant periapical osteolysis is caused by pulpal infection, and bacterial lipopolysaccharide (LPS) is known to play a major role in the bone resorption process. Little is known concerning the effect of root canal intervisit dressings on residual LPS in root canals after bacterial cell...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81199-4
更新日期：1993-02-01 00:00:00
abstract：INTRODUCTION:This study assessed apical extrusion during treatment with GentleWave (GW; Sonendo Inc, Laguna Hills, CA), a conventional open-ended 30-G needle (CN), or Endovac (EV; SybronEndo, Orange, CA) in root canals enlarged to different dimensions with and without apical constriction. METHODS:Sixteen mandibular mo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.04.009
更新日期：2016-01-01 00:00:00
abstract：:The present survey was conducted to obtain answers to some basic questions regarding the use of intracanal medications, root canal filling methods, and the intracanal breakage of instruments. A letter was sent to 300 endodontists listed in the 1995-1996 membership roster of the American Association of Endodontists. Ei...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80306-9
更新日期：1999-12-01 00:00:00
abstract：:The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80395-1
更新日期：1999-01-01 00:00:00
abstract：:Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study,...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200308000-00008
更新日期：2003-08-01 00:00:00
