Abstract:
:Hepatocyte growth factor (HGF) is a potent mitogenic and angiogenic factor involved in the development of many tissues including the teeth. We previously reported that dental pulp cells express the HGF receptor c-met and that HGF can enhance cell proliferation and differentiation. The purpose of this study was to determine whether HGF could stimulate proliferation and/or differentiation of murine dental papilla cells (MDPCs). MDPCs were isolated from neonatal mice, and the mitogenic potential of HGF on MDPCs was measured by 3-(4, 5-dimethyl-thyazol-2-yl)-2, 5-diphenyltetrazolium and flow cytometry. Differentiation of MDPCs in the presence of HGF was characterized by mineralization assay, alkaline phosphatase (ALP) activity and reverse-transcription polymerase chain reaction. We reported that MDPCs expressed both HGF and c-met and that HGF enhanced proliferation of MDPCs by concentrations ranging from 5 ng/mL to 20 ng/mL, with 10 ng/mL as the optimal concentration (p < 0.05). HGF prolonged S phase and shortened G1 phase of MDPCs, increased ALP activity significantly over the control (p < 0.05), and enhanced the formation of mineral nodules. ALP, dentin sialophosphoprotein, and dentin matrix protein-1 expression were significantly increased in the presence of HGF (p < 0.05), as were the expression of Msx1, Runx2, and Pax9. Our data suggest that HGF plays an important role in tooth development, by promoting the proliferation and differentiation of MDPCs.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Li R,Peng L,Ren L,Tan H,Ye Ldoi
10.1016/j.joen.2008.11.031subject
Has Abstractpub_date
2009-03-01 00:00:00pages
382-8issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(08)01104-7journal_volume
35pub_type
杂志文章abstract::The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200012000-00011
更新日期:2000-12-01 00:00:00
abstract::In the present study, we investigated the effect of the addition of different grades of gum rosins and hydrogenated resins to Grossman cement on dimensional stability, solubility and disintegration. pH and conductivity, which may affect these properties, were also determined. The experiments were performed according t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80284-2
更新日期:1999-07-01 00:00:00
abstract::Twenty volunteers with first molars free of restorations and caries were recruited. One molar from each arch was selected, and rubber dam was applied. Seven sites on each crown were electric pulp tested 4 times with an Elements Diagnostic Unit, and lowest threshold responses were recorded. Data were analyzed with one-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.08.006
更新日期:2007-11-01 00:00:00
abstract:INTRODUCTION:Root resorption is a common complication after replantation following traumatic dental avulsion. Endodontic therapy combined with local and intracanal medications aims to avoid osteoclastic activity. In such cases, the application of alendronate (ALN), a bisphosphonate widely used for the treatment of bone...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.07.010
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.001
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:Securing a reproducible glide path before instrumentation is recommended to maintain the original geometry of the root canal system and to prevent file separation. Mechanical glide path management systems have been introduced to expedite this step. The aim of this study was to compare apical transportation...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.07.014
更新日期:2015-11-01 00:00:00
abstract:INTRODUCTION:Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold ma...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.011
更新日期:2018-06-01 00:00:00
abstract:INTRODUCTION:Magnetic resonance imaging (MRI) has the potential to aid in determining the presence and extent of cracks/fractures in teeth because of better contrast without ionizing radiation. The objectives were to develop MRI criteria for root crack/fracture identification and to establish reliability and accuracy i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.008
更新日期:2019-06-01 00:00:00
abstract:INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the eff...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.06.004
更新日期:2013-09-01 00:00:00
abstract:INTRODUCTION:External apical root resorption (EARR) is a frequent iatrogenic effect of orthodontic treatment. The way root-filled teeth respond to orthodontic forces with respect to EARR has been reported as varying widely between individuals. Genetic variants in the interleukin-1 gene have been associated with an incr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.12.022
更新日期:2012-03-01 00:00:00
abstract:INTRODUCTION:The aim of the present study was to assess the bond strength of root fillings in oval-shaped canals prepared with the self-adjusting file (SAF) system. METHODS:A careful specimen selection resulted in 2 equal groups, each consisting of 12 extracted mandibular canines with oval canals that had vital pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.10.026
更新日期:2013-02-01 00:00:00
abstract::This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a sta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80521-2
更新日期:1995-10-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the radiopacity of calcium aluminate cement (EndoBinder) with 3 different radiopacifiers (bismuth oxide, zinc oxide, or zirconium oxide) in comparison with gray mineral trioxide aggregate (GMTA), white MTA, and dental structures (enamel and dentin). METHODS:Eighteen t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.10.001
更新日期:2011-01-01 00:00:00
abstract::The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80217-2
更新日期:1996-07-01 00:00:00
abstract:INTRODUCTION:This study evaluated the effects of three different endodontic sealers on the bond strength of a fiber post cemented with adhesive resin cement. METHODS:Forty-eight extracted maxillary central incisors were prepared with the step-back technique and randomly divided into four groups (n=12 for each group): ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.014
更新日期:2010-03-01 00:00:00
abstract::This study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. Microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. The canal contents were analyzed by aerobic/an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000148149.27636.9c
更新日期:2005-06-01 00:00:00
abstract::The purpose of this study was to evaluate a new periapical index based on cone beam computed tomography (CBCT) for identification of apical periodontitis (AP). The periapical index proposed in this study (CBCTPAI) was developed on the basis of criteria established from measurements corresponding to periapical radioluc...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.013
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:This study addressed the following population, intervention, comparator, outcome, timing, study design and setting question: in patients with preoperative pain who undergo single-visit nonsurgical endodontic treatment, what is the comparative efficacy of corticosteroids compared with other analgesics or pl...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.05.015
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:The aim of this in vitro study was to evaluate the depth of penetration of 4 different endodontic resin sealers into the radicular dentinal tubules with the aid of confocal microscopy. METHODS:Eighty single-rooted teeth were instrumented and divided into 4 groups composed of 20 teeth each. The samples were ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.05.017
更新日期:2012-10-01 00:00:00
abstract::This review considers oral trauma and its relationship to orthodontics with respect to prevention and primary, secondary, and tertiary care. The level of evidence is not high in regard to this topic, but recommendable approaches to trauma at each stage are discussed on the basis of available literature and published g...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2012.10.030
更新日期:2013-03-01 00:00:00
abstract::The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200007000-00001
更新日期:2000-07-01 00:00:00
abstract::The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO prod...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.07.012
更新日期:2007-11-01 00:00:00
abstract:INTRODUCTION:Previously, we have shown that stem cells from apical papilla (SCAPs) can be chemoattracted by stromal cell-derived factor-1α (SDF-1α). The purpose of this study was to investigate the intracellular signaling pathways involved in SDF-1α-mediated migration of SCAPs. METHODS:Chemotaxis assays were performed...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.04.014
更新日期:2016-07-01 00:00:00
abstract:INTRODUCTION:Warm vertically compacted gutta-percha obturation techniques use root canal sealers that are heated during the obturation. This study aims at investigating the suitability of selected sealers with warm gutta-percha obturation techniques. METHODS:The composition of an experimental sealer (Septodont; Saint ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.06.007
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.07.007
更新日期:2012-11-01 00:00:00
abstract:INTRODUCTION:In this study, we investigated if apical periodontitis (AP) associated with diabetes influenced the levels of endogenous antioxidants, the total antioxidant capacity (TAC), and the oxidant parameter in the serum of Wistar rats. METHODS:Forty male rats were divided into 4 equal groups: normal rats (N), rat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.05.014
更新日期:2017-10-01 00:00:00
abstract:INTRODUCTION:Endodontic diagnostic tests are often used clinically to assess pulp status as a basis for the diagnosis and determination of whether root canal treatment (RCT) is indicated. Response to cold and pain on percussion are 2 common tests, yet their validity in identifying nonvital pulp in regular dental practi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.03.016
更新日期:2016-06-01 00:00:00
abstract::The effect of two different root canal cements and removal of the smear layer on the apical seal in roots obturated with Thermafil obturators was tested. Thirty extracted human mandibular first molars with 60 canals (mesial roots) were instrumented with the use of a step-back technique. Teeth were placed into 6 groups...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80131-3
更新日期:1998-05-01 00:00:00
abstract:INTRODUCTION:Idiopathic bone cavity (IBC) is an uncommon bone lesion that usually affects youngsters as an unilocular radiolucency with predilection for the posterior mandible. Because the lesion is frequently located in proximity to the adjacent teeth, chronic apical periodontitis is commonly included as a differentia...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.11.004
更新日期:2021-02-01 00:00:00
abstract::Strippings are problems that are frequent on thin and concave roots. Treatment and prognosis differ from that of a lateral root perforation because of the size, oval shape, and thin edges of the striping. We propose a two-step technique: an endodontic phase in which the root canal system is sealed with gutta-percha ov...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80069-0
更新日期:1996-12-01 00:00:00