Optical detection of hemoglobin in pulpal blood.

Abstract:

:An in vitro, flow-through optical system was designed to measure hemoglobin (Hb) concentrations in the pulp space. The system included light-emitting diodes and a silicon photodetector positioned on opposing surfaces of human teeth. A syringe pump allowed a controlled flow of blood through the pulp chamber. The Hb concentration was computed as a nonlinear function of transmitted light intensity. Transmitted light intensities were also used as indicators of oxygenation level. Optical measurements correlated with Hb values measured by the conventional cyanmethemoglobin method (r=0.993). The mean percentage error was 5.8%, and the standard error of prediction was 0.77 g/dl for Hb concentrations ranging from 4 to 20 g/dl. Deoxygenated blood exhibited up to 31% lower transmitted intensity. Light transmission through teeth may be useful in the assessment of total Hb and blood oxygenation within the pulp chamber.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Diaz-Arnold AM,Arnold MA,Wilcox LR

doi

10.1016/S0099-2399(96)80230-5

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

19-22

issue

1

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(96)80230-5

journal_volume

22

pub_type

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