Stimulatory effect of laser irradiation on calcified nodule formation in human dental pulp fibroblasts.

Abstract:

:The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased the number of calcified nodules in a time-dependent manner. The activity of alkaline phosphatase and production of collagen and osteocalcin in conditioned medium were measured. Both were higher in the irradiated group than in the nonirradiated group. These results suggested that formation of calcified nodules in HDP cells, as well as in alkaline phosphatase activity, the production of collagen and osteocalcin were enhanced by laser irradiation.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Ohbayashi E,Matsushima K,Hosoya S,Abiko Y,Yamazaki M

doi

10.1016/S0099-2399(99)80395-1

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

30-3

issue

1

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(99)80395-1

journal_volume

25

pub_type

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