Abstract：
INTRODUCTION:Complement activation is considered a major mechanism in innate immunity. Although it is mainly involved in initiating inflammation, recent data reported its involvement in other processes such as tissue regeneration. In the dental pulp, complement C5a fragment has been shown to be involved in the recruitment of dental pulp stem cells (DPSCs). This study sought to investigate the possible role of C3a, another complement fragment, in the early steps of dentin-pulp regeneration. METHODS:Expression of C3a receptor (C3aR) was investigated by immunofluorescence and reverse transcriptase polymerase chain reaction on cultured pulp fibroblasts, STRO-1-sorted DPSCs, as well as on human tooth sections in vivo. The effect of C3a on proliferation of both DPSCs and pulp fibroblasts was investigated by MTT assay. Cell migration under a C3a gradient was investigated by using microfluidic chemotaxis chambers. RESULTS:C3aR was expressed in vivo as well as in cultured pulp fibroblasts co-expressing fibroblast surface protein and in DPSCs co-expressing STRO-1. Addition of recombinant C3a induced a significant proliferation of both cell types. When subjected to a C3a gradient, DPSCs were mobilized but not specifically recruited, whereas pulp fibroblasts were specifically recruited following a C3a gradient. CONCLUSIONS:These results provide the first demonstration of C3aR expression in the dental pulp and demonstrate that C3a is involved in increasing DPSCs and fibroblast proliferation, in mobilizing DPSCs, and in specifically guiding fibroblast recruitment. This provides an additional link to the tight correlation between inflammation and tissue regeneration.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Rufas P,Jeanneau C,Rombouts C,Laurent P,About Idoi
10.1016/j.joen.2016.06.011subject
Has Abstractpub_date
2016-09-01 00:00:00pages
1377-84issue
9eissn
0099-2399issn
1878-3554pii
S0099-2399(16)30366-1journal_volume
42pub_type
杂志文章abstract：:The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.021
更新日期：2007-08-01 00:00:00
abstract：:Prostaglandins (PG) have been implicated in the genesis of periapical lesions. Periapical specimens from patients with clinical signs and symptoms of chronic and acute apical periodontitis were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars were frozen and used as...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81737-1
更新日期：1991-03-01 00:00:00
abstract：INTRODUCTION:Fractured endodontic files present a major problem. A novel method has been proposed to retrieve fractured nickel-titanium (NiTi) endodontic files by using electrochemical dissolution. However, the effect of file dissolution on adjacent soft tissues such as the periodontal ligament (PDL) has not been inves...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.12.013
更新日期：2013-05-01 00:00:00
abstract：INTRODUCTION:Many anatomical variations can occur within the mandibular first molar. Commonly, 3-4 canals are located, but as many as 6-7 canals have been reported. METHODS:This report describes a case of a mandibular first molar with 6 separate canals (3 mesial and 3 distal) that was instrumented with conventional ha...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.03.005
更新日期：2011-06-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the accuracy of the Root ZX II Apex Locator (RZX), the Elements Apex Locator (ELE), and the Precision Apex Locator (PAL). METHODS:Forty single-rooted extracted teeth were decoronated and the root canals coronally flared. Actual canal lengths were determined by inse...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.09.016
更新日期：2010-02-01 00:00:00
abstract：INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.04.028
更新日期：2010-08-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.11.007
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to analyze the features of lesions obtained from biopsies at the periapical area of teeth with a radiographic or clinical initial diagnosis of apical periodontitis. METHODS:A retrospective study was conducted on biopsies obtained from 1953-2018 at 3 Brazilian oral and maxillo...
journal_title：Journal of endodontics
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joen.2020.01.003
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:Passive ultrasonic irrigation (PUI) is the most widespread method used to activate irrigation solutions. Concerns have been raised that PUI is less effective in curved root canals and is not passive at all. Our aim was to compare a novel passive sonic irrigation (PSI) device (6000 Hz) with PUI and manual i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.08.024
更新日期：2016-12-01 00:00:00
abstract：:The diameter of nine sizes of three brands of endodontic spreaders was measured at D1 and D16 and compared with corresponding sizes of six brands of accessory gutta-percha cones. Two evaluators independently measured 20 randomly selected samples of each size of finger spreader and accessory gutta-percha cone. A standa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(07)80153-1
更新日期：1991-01-01 00:00:00
abstract：:This case report describes an innovative virtual simulation method using a computer-aided rapid prototyping (CARP) model and a computer-aided design (CAD) program for autotransplantation of an immature third molar.A compromised left mandibular second molar (#18) was extracted and replaced by autotransplantation using ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.08.010
更新日期：2018-12-01 00:00:00
abstract：INTRODUCTION:Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the rate of dentin accumulation. METHODS:Histology, immunohistochemistry, vital dye labeling, and histomorphometric assays were used to quant...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.07.014
更新日期：2019-11-01 00:00:00
abstract：INTRODUCTION:During the dental pulp repair process, human dental pulp cells (HDPCs) migrate to injury sites where they may differentiate into odontoblastlike cells. WNT6 plays a role in dental development and can activate a noncanonical pathway including the c-Jun N-terminal kinase (JNK) pathway. The mechanism of WNT6 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.12.023
更新日期：2014-07-01 00:00:00
abstract：INTRODUCTION:This clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [LPSs]) found in primary and secondary endodontic infections with apical periodontitis by correlating LPS contents with clinical/radiographic findings. In addition, the presence of target gram-negative anaerobic bact...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.04.021
更新日期：2012-08-01 00:00:00
abstract：INTRODUCTION:Various materials have been advocated for use as root-end filling materials. The purpose of the present in vitro study was to compare the cytotoxicity of 4 root-end filling materials: glass ionomer cement (GIC; Fuji II, GC Corp, Tokyo, Japan), reinforced zinc oxide-eugenol cement (IRM; Dentsply Tulsa Denta...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.09.004
更新日期：2012-12-01 00:00:00
abstract：:The aim of this study was to investigate fracture patterns occurring when a human upper central incisor is subjected to impact loadings at various angles. A two-dimensional finite element (FE) model of the maxillary incisor and surrounding tissues was established. The structural damping factor for the tooth was then c...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000157992.29221.41
更新日期：2005-10-01 00:00:00
abstract：INTRODUCTION:This study used micro-computed tomographic imaging to compare the shaping ability of Mtwo (VDW, Munich, Germany), a conventional nickel-titanium file system, and Reciproc (VDW), a reciprocating file system morphologically similar to Mtwo. METHODS:Root canal shaping was performed on the mesiobuccal and dis...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.12.032
更新日期：2014-08-01 00:00:00
abstract：:It is thought that externally applied bleaching agents may penetrate into the pulp chamber. This study was conducted to evaluate the diffusion of peroxide bleaching agents into the pulp chamber of teeth restored with various restorative materials. Sixty-five human extracted anterior maxillary teeth were separated into...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200002000-00008
更新日期：2000-02-01 00:00:00
abstract：INTRODUCTION:Communication between pulp and periodontal tissue has been well established. However, it is unknown when periodontal disease begins to affect the clinical response of pulp tissue. The aim of this study was to assess the influence of periodontal severity on pulp sensibility by means of electric and thermal ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.07.006
更新日期：2017-11-01 00:00:00
abstract：INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesize...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.015
更新日期：2019-01-01 00:00:00
abstract：:Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2014.01.017
更新日期：2014-04-01 00:00:00
abstract：:Plasminogen activator converts plasminogen to plasmin, and plasmin activates the latent matrix metalloproteinases. Tissue plasminogen activator (t-PA) is one of the important proteolysis factors present in human inflamed tissues. However, few studies reported on the mechanisms of tissue destruction via a t-PA proteoly...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200302000-00007
更新日期：2003-02-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare 2 reciprocating nickel-titanium systems with different manufacturing characteristics and to evaluate the influence of simulated clinical use on their torsional resistance. METHODS:New 25/.08 WaveOne Primary (WO; Dentsply Sirona, York, PA) and 25/.07 WaveOne Gold Primar...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.04.009
更新日期：2019-08-01 00:00:00
abstract：INTRODUCTION:The purpose of this retrospective study was to determine the success of the inferior alveolar nerve (IAN) block using either 3.6 mL or 1.8 mL 2% lidocaine with 1:100,000 epinephrine in patients presenting with symptomatic irreversible pulpitis. METHODS:As part of 7 previously published studies, 319 emerge...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.007
更新日期：2013-08-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the bond strength of different adhesive sealers on Resilon and gutta-percha. METHODS:Pellets of gutta-percha and Resilon were embedded into test tubes. Small eyelets were attached to those surfaces with a 0.5-mm film of different root canal sealers. Real Seal, Real Se...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.01.004
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Dens invaginatus is a critical condition for endodontic treatment once it frequently presents a complex internal anatomy and might be associated with incomplete root and apical development. METHODS:Three cases of dens invaginatus with open apex in maxillary lateral incisors are presented. In the first cas...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2010.02.002
更新日期：2010-06-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effects of endodontic irrigants on the push-out strength and hydration behavior of accelerated mineral trioxide aggregate (MTA) in its early setting phase. METHODS:In an in vitro perforation model, MTA with or without 10% CaCl(2) was condensed and allowed to i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.039
更新日期：2010-12-01 00:00:00
abstract：:Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from h...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200203000-00003
更新日期：2002-03-01 00:00:00
abstract：:The objective of this study was to compare the sealing ability of ActiV GP/glass ionomer (GI) sealer (Brasseler USA, Savannah, GA), Resilon/Epiphany (Pentron Clinical Technologies, Wallingford, CT), and gutta-percha (GP)/AH Plus (Dentsply Maillefer, Tulsa, OK). Seventy-three human single-rooted teeth were randomly div...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.03.006
更新日期：2008-06-01 00:00:00
abstract：:The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 x 1 mm). For cytotoxicity testing (MTT method), the spe...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.05.006
更新日期：2006-10-01 00:00:00
