Cytotoxicity of newly developed ortho MTA root-end filling materials.

Abstract:

INTRODUCTION:Various materials have been advocated for use as root-end filling materials. The purpose of the present in vitro study was to compare the cytotoxicity of 4 root-end filling materials: glass ionomer cement (GIC; Fuji II, GC Corp, Tokyo, Japan), reinforced zinc oxide-eugenol cement (IRM; Dentsply Tulsa Dental, Tulsa, OK), and 2 types of mineral trioxide aggregate. METHODS:This study used MG-63 cells derived from a human osteosarcoma. To quantitatively evaluate the cytotoxicity of test materials, the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Each specimen was examined by scanning electron microscopy for the observation of cell morphology. RESULTS:The XTT assay showed that the cell viability of ProRoot MTA (Dentsply Tulsa Dental) was higher than that of GIC and Ortho MTA (BioMTA, Seoul, Republic of Korea) at all time points. IRM showed significantly lower cell viability than the other groups. The scanning electron microscopic analysis revealed that elongated, dense, and almost confluent cells were observed in the cultures of GIC, Ortho MTA, and ProRoot MTA specimens. In contrast, cells on the surface of IRM were rounded in shape, and the numbers and the density of the cells were smaller than that in the other groups. CONCLUSIONS:ProRoot MTA and GIC showed good biocompatibility in this study. However, Ortho MTA showed lower biocompatibility compared with ProRoot MTA and GIC.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Lee BN,Son HJ,Noh HJ,Koh JT,Chang HS,Hwang IN,Hwang YC,Oh WM

doi

10.1016/j.joen.2012.09.004

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1627-30

issue

12

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(12)00870-9

journal_volume

38

pub_type

杂志文章
  • Effect of different grades of gum rosins and hydrogenated resins on the solubility, disintegration, and dimensional alterations of Grossman cement.

    abstract::In the present study, we investigated the effect of the addition of different grades of gum rosins and hydrogenated resins to Grossman cement on dimensional stability, solubility and disintegration. pH and conductivity, which may affect these properties, were also determined. The experiments were performed according t...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(99)80284-2

    authors: Sousa-Neto MD,Guimarães LF,Saquy PC,Pécora JD

    更新日期:1999-07-01 00:00:00

  • Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm.

    abstract:INTRODUCTION:The purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (AgNPs) as an irrigant or medicament against Enterococcus faecalis biofilms formed on root dentin. METHODS:Dentin sections were inoculated with E. faecalis for 4 weeks to establish a standard monospecies biofilm m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.08.022

    authors: Wu D,Fan W,Kishen A,Gutmann JL,Fan B

    更新日期:2014-02-01 00:00:00

  • Procedural accidents: an online study guide.

    abstract::The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,评审

    doi:10.1016/j.joen.2007.07.002

    authors: JOE Editorial Board.

    更新日期:2008-05-01 00:00:00

  • Effects of short-term chemical disinfection of gutta-percha cones: identification of affected microbes and alterations in surface texture and physical properties.

    abstract::The aims of this study were to identify microorganisms on the surface of gutta-percha (GP) cones using a polymerase chain reaction (PCR) and to evaluate the sterilization effect of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and ChloraPrep on these bacteria. Alterations of the surface texture and physic...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2007.01.019

    authors: Pang NS,Jung IY,Bae KS,Baek SH,Lee WC,Kum KY

    更新日期:2007-05-01 00:00:00

  • The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis.

    abstract::Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000164855.98346.fc

    authors: George S,Kishen A,Song KP

    更新日期:2005-12-01 00:00:00

  • Evaluation of root canal configuration of mandibular molars in a Brazilian population by using cone-beam computed tomography: an in vivo study.

    abstract:INTRODUCTION:The aim of this study was to analyze and characterize root canal morphology of mandibular molars of the Brazilian population by using cone-beam computed tomography (CBCT). METHODS:Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning w...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.04.030

    authors: Silva EJ,Nejaim Y,Silva AV,Haiter-Neto F,Cohenca N

    更新日期:2013-07-01 00:00:00

  • Periapical Microsurgery: Assessment of Different Types of Light-emitting Diode Transilluminators in Detection of Dentinal Defects.

    abstract:INTRODUCTION:This study evaluated 4 different light-emitting diode (LED) transilluminators and the impact of operator experience in the detection of dentinal defects through an ex vivo TRUEJAW surgical model (Dental Engineering Laboratories, Santa Barbara, CA). METHODS:Forty-four extracted and endodontically treated m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.10.031

    authors: Arnarsdottir EK,Karunanayake GA,Pettit NE,Phillips C,Saemundsson SR,Tawil PZ

    更新日期:2020-02-01 00:00:00

  • Anesthetic efficacy of a primary articaine infiltration and a repeat articaine infiltration in the incisive/mental nerve region of mandibular premolars: a prospective, randomized, single-blind study.

    abstract:INTRODUCTION:The authors conducted a prospective, randomized, single-blind study to determine the degree of pulpal anesthesia obtained with a primary infiltration of 1 cartridge of articaine in the incisive/mental nerve region of the mandibular second premolar and to determine the anesthetic efficacy of a repeat artica...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2012.11.039

    authors: Dressman AS,Nusstein J,Drum M,Reader A

    更新日期:2013-03-01 00:00:00

  • Sodium Hypochlorite and a Preparation Containing Glycocholic Acid and Surfactants Have a Synergistic Action on Organic Tissue Dissolution In Vitro.

    abstract:INTRODUCTION:The aim of this study was to evaluate porcine palatal mucosa dissolution by sodium hypochlorite (NaOCl) with or without an auxiliary dissolving agent containing glycocholic acid and a mixture of surfactants (Keratobacter [KB]; Saint Joseph DID, Valencia, Spain). METHODS:One hundred forty samples were obta...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2018.01.007

    authors: Peña López A,Conde AJ,Estevez R,Valencia de Pablo O,Rossi-Fedele G,Cisneros R

    更新日期:2018-05-01 00:00:00

  • Revascularization Technique for the Treatment of External Inflammatory Root Resorption: A Report of 3 Cases.

    abstract::The current external inflammatory root resorption treatment protocol, which uses calcium hydroxide dressing, usually comprises multiple and long-term applications. In addition to the need for multiple appointments for calcium hydroxide replacement, the long-term maintenance of this compound in the root canal weakens d...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.03.019

    authors: Santiago CN,Pinto SS,Sassone LM,Hirata R Jr,Fidel SR

    更新日期:2015-09-01 00:00:00

  • Outcome of Endodontic Surgery: A Meta-analysis of the Literature-Part 3: Comparison of Endodontic Microsurgical Techniques with 2 Different Root-end Filling Materials.

    abstract:INTRODUCTION:The aim of the present study was to investigate the influence of root-end preparation and filling material on endodontic surgery outcome. A systematic review and meta-analysis was conducted to determine the outcome of resin-based endodontic surgery (RES, the use of high-magnification preparation of a shall...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,meta分析,评审

    doi:10.1016/j.joen.2018.02.021

    authors: Kohli MR,Berenji H,Setzer FC,Lee SM,Karabucak B

    更新日期:2018-06-01 00:00:00

  • Influence of injection speed on the effectiveness of incisive/mental nerve block: a randomized, controlled, double-blind study in adult volunteers.

    abstract::This randomized, double-blind trial tested the null hypothesis that speed of deposition has no influence on the injection discomfort, efficacy, distribution, and duration of pulp anesthesia after incisive/mental nerve block in adult volunteers. Thirty-eight subjects received incisive/mental nerve blocks of 2.0 mL lido...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2007.07.016

    authors: Whitworth JM,Kanaa MD,Corbett IP,Meechan JG

    更新日期:2007-10-01 00:00:00

  • Penetration of constricted canals with modified K files.

    abstract::A technique using K files with modified tips has been developed for the penetration of constricted canals. The tip of a #10 K file was sliced diagonally to make it thinner. Because this modified K file has an especially fine tip and an appropriate stiffness, it followed constricted or sharply curved canals well and ha...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(97)80190-2

    authors: Kobayashi C

    更新日期:1997-06-01 00:00:00

  • The effect of instrumentation with two different file types, each with 2.5% NaOCl irrigation on the microhardness of root dentin.

    abstract::The aim of this study was to measure root-dentin microhardness after instrumentation with two types of files and using irrigation with 2.5% sodium hypochlorite. Thirty roots were instrumented with irrigation: 10 roots had the pulp extirpated only; 10 roots were instrumented with stainless steel files, and 10 roots wer...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200204000-00012

    authors: Slutzky-Goldberg I,Liberman R,Heling I

    更新日期:2002-04-01 00:00:00

  • Buffered lidocaine for incision and drainage: a prospective, randomized double-blind study.

    abstract:INTRODUCTION:Buffered local anesthetics have not been studied for incision and drainage procedures in dentistry. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and pain of an incision and drainage procedure by using a buffered versus a nonbuffered 2% lidocaine wi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2013.07.008

    authors: Balasco M,Drum M,Reader A,Nusstein J,Beck M

    更新日期:2013-11-01 00:00:00

  • Subcutaneous connective tissue reaction to methacrylate resin-based and zinc oxide and eugenol sealers.

    abstract:INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2010.06.019

    authors: Zmener O,Pameijer CH,Kokubu GA,Grana DR

    更新日期:2010-09-01 00:00:00

  • Comparison of apical leakage in teeth obturated with a polyamide varnish or zinc oxide and eugenol cement using lateral condensation.

    abstract::The sealing ability of copal varnish has been investigated when used as an adjunct in restorative procedures, retrofilling procedures, and dontic obturation procedures. Barrier, a polyamide-type polymer cavity varnish, has been compared with copal varnishes in restorative dentistry. This investigation compared the api...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81138-6

    authors: Smith DW,Wong M

    更新日期:1992-01-01 00:00:00

  • Reinforcement of immature roots with a new resin filling material.

    abstract::The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2005.08.001

    authors: Stuart CH,Schwartz SA,Beeson TJ

    更新日期:2006-04-01 00:00:00

  • Quantification of endotoxins in infected root canals and acute apical abscess exudates: monitoring the effectiveness of root canal procedures in the reduction of endotoxins.

    abstract:INTRODUCTION:This clinical study was conducted to measure the endotoxin levels in infected root canals (RCs) and exudates related to acute apical abscesses (AAAs). In addition, the effectiveness of RC procedures in reducing the endotoxin levels in RCs was monitored. METHODS:Paired samples of infected RCs and exudates ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.10.008

    authors: Sousa EL,Martinho FC,Nascimento GG,Leite FR,Gomes BP

    更新日期:2014-02-01 00:00:00

  • In vitro measurement accuracy of an electronic apex locator in teeth with simulated apical root resorption.

    abstract::The purpose of this in vitro study was to evaluate the accuracy of Root ZX apex locator to determine the working length in teeth with simulated apical root resorption. Fifty extracted, single-rooted, human teeth with mature apices were used in this study. An irregular cavity defect was drilled at the apex of each toot...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200206000-00011

    authors: Goldberg F,De Silvio AC,Manfré S,Nastri N

    更新日期:2002-06-01 00:00:00

  • Effectiveness of ProFile .04 taper rotary instruments in endodontic retreatment.

    abstract::This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200002000-00010

    authors: Sae-Lim V,Rajamanickam I,Lim BK,Lee HL

    更新日期:2000-02-01 00:00:00

  • Dental anxiety among patients undergoing endodontic treatment.

    abstract::The purpose of this study was to evaluate the dental anxiety expressed among patients undergoing endodontic treatment with respect to some social and dental parameters. The dental records of 98 patients, 42 men and 56 women who were referred for root canal treatment to an endodontist, were examined. Patients completed...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80028-9

    authors: Peretz B,Moshonov J

    更新日期:1998-06-01 00:00:00

  • The effect of antibiotics and endodontic antimicrobials on the polymerase chain reaction.

    abstract::The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000152899.54247.4e

    authors: Fouad AF,Barry J

    更新日期:2005-07-01 00:00:00

  • Cracked tooth syndrome: characteristics and distribution among adults in a Nigerian teaching hospital.

    abstract::This study highlighted the characteristics and distribution of cracked tooth syndrome (CTS) and the associated factors in adult attendees in the University of Nigeria Teaching Hospital. Three hundred seventy patients aged 18 years to 77 years with CTS-like conditions were included and studied over 12 months. The follo...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2008.12.011

    authors: Udoye CI,Jafarzadeh H

    更新日期:2009-03-01 00:00:00

  • 2-Hydroxyethyl methacrylate inhibits migration of dental pulp stem cells.

    abstract:INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the eff...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.06.004

    authors: Williams DW,Wu H,Oh JE,Fakhar C,Kang MK,Shin KH,Park NH,Kim RH

    更新日期:2013-09-01 00:00:00

  • Influence of different root canal-filling materials on the mechanical properties of root canal dentin.

    abstract::The aims of this study were to compare Resilon (Resilon Research LLC, Madison, CT) in conjunction with either a bonding (Epiphany; Pentron Clinical Technologies, Wallingford, CT) or a nonbonding endodontic sealer (Pulp Canal Sealer; Kerr Corporation, Orange, CA) to EndoRez (Ultradent Products Inc, South Jordan, UT) an...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2007.02.018

    authors: Grande NM,Plotino G,Lavorgna L,Ioppolo P,Bedini R,Pameijer CH,Somma F

    更新日期:2007-07-01 00:00:00

  • Factors affecting apical leakage assessment.

    abstract::This study was conducted to evaluate the influence of immediate versus delayed immersion time, and passive dye immersion versus centrifuged dye on apical leakage measurements. Eighty-four extracted human teeth with single straight canals were instrumented and divided into four experimental groups of 20 teeth each plus...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81364-6

    authors: Karagöz-Küçükay I,Küçükay S,Bayirli G

    更新日期:1993-07-01 00:00:00

  • Root canal anatomy preservation of WaveOne reciprocating files with or without glide path.

    abstract:INTRODUCTION:This study evaluated the influence of glide path on canal curvature and axis modification after instrumentation with WaveOne Primary reciprocating files. METHODS:Thirty ISO 15, 0.02 taper Endo Training Blocks were used. In group 1, glide path was created with PathFile 1, 2, and 3 at working length, wherea...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.09.030

    authors: Berutti E,Paolino DS,Chiandussi G,Alovisi M,Cantatore G,Castellucci A,Pasqualini D

    更新日期:2012-01-01 00:00:00

  • A method to determine the location and shape of an intracoronal bleach barrier.

    abstract::The external cervical root resorption associated with intracoronal bleaching of pulpless teeth can be a devastating lesion. It often cannot be repaired. To prevent this problem, increasing attention has been focused on placing a barrier between the pulp chamber and the endodontic filling material. The objective of thi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80822-8

    authors: Steiner DR,West JD

    更新日期:1994-06-01 00:00:00

  • Three computer methods to reconstruct pulpal blood vessels and nerves.

    abstract::This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a sta...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80521-2

    authors: Lyroudia K,Nikolaidis N,Pitas I,Palakidis K

    更新日期:1995-10-01 00:00:00