Abstract：
INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the effects of 2-hydroxyethyl methacrylate (HEMA) on the migration of dental pulp stem cells (DPSC) in vitro. METHODS:Cell viability was assessed using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay, and cell migration was evaluated using the wound scratch assay and transwell migration assay at noncytotoxic doses. The Western blot was used to examine pathways associated with migration such as focal adhesion kinase, mitogen-activated protein kinase, and glycogen synthase kinase 3. RESULTS:There were no drastic changes in the cell viability below 3 mmol/L HEMA. When DPSCs were treated with HEMA at 0.5, 1.0, and 2.5 mmol/L, cell migration was diminished. HEMA-treated DPSCs exhibited the loss of phosphorylated focal adhesion kinase in a dose-dependent manner. The HEMA-mediated inhibition of cell migration was associated with phosphorylation of p38 but not glycogen synthase kinase 3, Extracellular signal-related kinase (ERK), or c-Jun N-terminal kinase (JNK) pathways. When we inhibited the p38 signaling pathway using a p38 inhibitor, the migration of DPSCs was suppressed. CONCLUSIONS:HEMA inhibits the migration of dental pulp cells in vitro, suggesting that poor pulpal wound healing under resin-based dental materials may be caused, in part, by the inhibition of cell migration by HEMA.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Williams DW,Wu H,Oh JE,Fakhar C,Kang MK,Shin KH,Park NH,Kim RHdoi
10.1016/j.joen.2013.06.004subject
Has Abstractpub_date
2013-09-01 00:00:00pages
1156-60issue
9eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00541-4journal_volume
39pub_type
杂志文章abstract：:Biopsy reports of specimens from the apices of 1659 teeth physically present at the time the biopsy was performed were reviewed. The following information was recorded from each report: (a) source of specimen, (b) sex of patient, (c) age of patient, (d) location of tooth associated with lesion, and (e) diagnoses repor...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/s0099-2399(06)81679-1
更新日期：1990-05-01 00:00:00
abstract：INTRODUCTION:The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. T...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.016
更新日期：2017-07-01 00:00:00
abstract：:A unique case of a mandibular molar with 5 canals is described. Literature pertaining to the analysis of the morphology of mandibular first molars is reviewed. Clinicians should thoroughly examine the pulpal floor and radiographs for the possibility of additional canals. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80211-7
更新日期：1997-01-01 00:00:00
abstract：:This case-control study sought to identify variables predictive of subsequent root canal therapy (RCT) in teeth receiving full coverage restorations. The University of North Carolina School of Dentistry's computerized treatment database was used to identify all patients receiving a single-unit crown on a nonendodontic...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.11.001
更新日期：2006-08-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the fracture strength of roots instrumented with the Self-Adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) and the ProTaper system (Dentsply Maillefer, Ballaigues, Switzerland) and filled with the cold lateral compaction technique. METHODS:In total, 50 mandibul...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.030
更新日期：2014-04-01 00:00:00
abstract：:This study examined the effects of calcium-depleting endodontic irrigants, ethylenediamine tetra-acetic acid (EDTA) and BioPure MTAD, on the dissolution, surface characteristics, and ultrastructural characteristics of white mineral trioxide aggregate (MTA). The latter was mixed in a 0.35 water-cement ratio, condensed ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.12.002
更新日期：2007-04-01 00:00:00
abstract：INTRODUCTION:Studies have found women try to avoid pain more than men, accept it less, and fear it more. The role of operator gender on subject gender pain in a clinical setting needs further study. The purpose of this randomized, double-blind investigation was to evaluate operator gender and its influence on reported ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2014.10.001
更新日期：2015-02-01 00:00:00
abstract：:This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.016
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:Pure gutta-percha (trans-1, 4-polyisoprene [TPI]) has been used extensively as a main component of gutta-percha for root canal filling. TPI has the interesting shape memory property by cross-linking, and this polymer was commercialized under the product name of SMP-2 (Kuraray Corp, Kashima, Japan). Therefo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.05.003
更新日期：2014-10-01 00:00:00
abstract：:The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80217-2
更新日期：1996-07-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare the fracture resistance of cast posts versus the fracture resistance of fiber posts by means of meta-analysis when they were used in the restoration of endodontically treated teeth. METHODS:MEDLINE, Cochrane Controlled Trials Register, China National Knowledge Infrastr...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joen.2012.09.026
更新日期：2013-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the accuracy of cone-beam computed tomographic (CBCT) to measure dentin thickness and its potential of predicting the remaining dentin thickness after the removal of fractured instrument fragments. METHODS:Twenty-three human mandibular molars were selected, and 4-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.041
更新日期：2017-09-01 00:00:00
abstract：PURPOSE:To evaluate in vitro the solubility of the Epiphany endodontic filling material Epiphany (Pentron Clinical Technologies, Wallingford, CT) prepared with its resinous solvent. METHODS:The specimens were prepared in the following experimental conditions: (1) GI, epiphany without photoactivation; (2) GII, Epiphany...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.021
更新日期：2009-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in regulating tricalcium silicate (C3S)-driven proliferation and biomineralization of human dental pulp cells (hDPCs) in vitro. METHODS:Human DPCs were cultured in C3S-containing medium and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.03.002
更新日期：2013-08-01 00:00:00
abstract：:The biocompatibility and apical microleakage of tricalcium phosphate based Sankin Apatite (SA) Type I, II, and III root canal sealers were investigated. Teflon tubes containing freshly mixed test materials were implanted in the subcutaneous tissue of mice. The observation periods were 24 h, 7, and 30 days, after which...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(97)80255-5
更新日期：1997-02-01 00:00:00
abstract：:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00004
更新日期：2004-03-01 00:00:00
abstract：INTRODUCTION:Enterococcus faecalis is frequently recovered from root-filled teeth with refractory apical periodontitis. The ability of E. faecalis to form a matrix-encased biofilm contributes to its pathogenicity; however, the role of extracellular dextran and DNA in biofilm formation and its effect on the susceptibili...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.04.007
更新日期：2012-07-01 00:00:00
abstract：:The purpose of this study was to compare the wicking propensity of multifilament sutures. Dexon II, Vicryl, and black silk suture (BSS) were dipped in saline or soaked for 48 h, then suspended on a microscope slide. Fluorescein isothiocyanate-dextran (FITC-D) was placed at the suture mid points, and its movement was o...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000121617.67923.05
更新日期：2004-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the ability of MTA (Dentsply Maillefer, Tulsa, OK) and EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA) to set in the presence of human blood and minimal essential media. METHODS:A model was created using polymethyl methacrylate blocks each pr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.041
更新日期：2013-08-01 00:00:00
abstract：INTRODUCTION:Squamous odontogenic tumor-like epithelial islands occurring in the walls of odontogenic cysts are histologically identical to the squamous odontogenic tumor (SOT). Microscopically, the squamous odontogenic tumor-like proliferations (SOTLPs) share certain histologic features with SOT, acanthomatous and des...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.02.010
更新日期：2011-05-01 00:00:00
abstract：:The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.11.005
更新日期：2007-01-01 00:00:00
abstract：:A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200206000-00001
更新日期：2002-06-01 00:00:00
abstract：INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.003
更新日期：2013-10-01 00:00:00
abstract：:A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81411-1
更新日期：1992-04-01 00:00:00
abstract：:Apical periodontitis and its concomitant periapical osteolysis is caused by pulpal infection, and bacterial lipopolysaccharide (LPS) is known to play a major role in the bone resorption process. Little is known concerning the effect of root canal intervisit dressings on residual LPS in root canals after bacterial cell...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81199-4
更新日期：1993-02-01 00:00:00
abstract：INTRODUCTION:Various factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.011
更新日期：2019-06-01 00:00:00
abstract：:In the rat, perforations of maxillary molars were created and treated with either tricalcium phosphate (Synthograft) or Cavit. At four time intervals (1 day, 1 wk, 2 wk, and 1 month), four evaluative factors (inflammation, bone resorption, cementum and dentin resorption, and epithelial proliferation) were analyzed and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(89)80171-2
更新日期：1989-09-01 00:00:00
abstract：INTRODUCTION:The aims of this study were to evaluate the vertical and horizontal relationships between the maxillary sinus floor (MSF) and the root apices of maxillary posterior teeth with various root configurations and the distance from the root apex to the MSF and the buccal cortical plate. METHODS:Serial axial, co...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.08.011
更新日期：2015-11-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) satisfies most of the ideal properties of a surgical root-end filling and perforation repair material. It has been found to be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable and promotes cementogenesis. The major...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：:This study sought to evaluate the influence of apical enlargement on the sealing ability of vertical compaction. Sixty anterior teeth were used. Half of these teeth were prepared to a size 25 file and the other half to a size 40 file. Then vertical compaction was performed. Apical microleakage was determined using pre...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80091-9
更新日期：1994-07-01 00:00:00
