Abstract：
INTRODUCTION:The purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (AgNPs) as an irrigant or medicament against Enterococcus faecalis biofilms formed on root dentin. METHODS:Dentin sections were inoculated with E. faecalis for 4 weeks to establish a standard monospecies biofilm model. These biofilms were tested in 2 stages. In stage 1, the biofilms were irrigated with 0.1% AgNP solution, 2% sodium hypochlorite, and sterile saline for 2 minutes, respectively. In stage 2, the biofilms were treated with AgNP gel (0.02% and 0.01%) and calcium hydroxide for 7 days. The ultrastructure of one half of the specimens from each group was evaluated by using scanning electron microscopy, whereas the structure and distribution of viable bacteria of the other half of the specimens were assessed with confocal laser scanning microscopy combined with viability staining. RESULTS:Syringe irrigation with 0.1% AgNP solution did not disrupt the biofilm structure, and the proportion of viable bacteria in the biofilm structures was not different from that of the saline group (P > .05) but was less than that of the control group (P < .05). The biofilms treated with 0.02% AgNP gel as medicament significantly disrupted the structural integrity of the biofilm and resulted in the least number of post-treatment residual viable E. faecalis cells compared with 0.01% AgNP gel and calcium hydroxide groups (P < .05). CONCLUSIONS:The findings from this study suggested that the antibiofilm efficacy of AgNPs depends on the mode of application. AgNPs as a medicament and not as an irrigant showed potential to eliminate residual bacterial biofilms during root canal disinfection.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wu D,Fan W,Kishen A,Gutmann JL,Fan Bdoi
10.1016/j.joen.2013.08.022subject
Has Abstractpub_date
2014-02-01 00:00:00pages
285-90issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(13)00732-2journal_volume
40pub_type
杂志文章abstract：:The endodontic endosseous implant (EEI) enjoyed wide use in the United States after it was introduced in the 1960's. For various reasons including incorrect case selection, improper use of the materials, and poor preparation for the implant a high number of failures resulted. Following opposition by some members of th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81496-2
更新日期：1993-10-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the variations in root canal length (RCL) occurring during endodontic treatment stages (initial, preflared, and concluded) and correlate them with the accuracy of Root ZX II (RZX). METHODS:After coronal access, 26 mandibular molars had the apical foramen of the 52 me...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.07.022
更新日期：2016-11-01 00:00:00
abstract：INTRODUCTION:An ideal orthograde or retrograde filling material should seal the pathways of communication between the root canal system and its surrounding tissues. It should also be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable. Mineral trioxide aggregate ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2009.09.006
更新日期：2010-01-01 00:00:00
abstract：:The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200411000-00008
更新日期：2004-11-01 00:00:00
abstract：INTRODUCTION:Calcium hydroxide (CH) and nano-calcium hydroxide (NCH) as intracanal medicaments may affect the physical and chemical properties of dentin. The aim of this investigation was to evaluate the effects of CH and NCH on the microhardness and superficial chemical structure of radicular dentin. METHODS:In this ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.06.002
更新日期：2019-09-01 00:00:00
abstract：INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesize...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.015
更新日期：2019-01-01 00:00:00
abstract：INTRODUCTION:The mental foramen (MF) is an important landmark in dentistry. Knowledge of its position is central to perform block anesthesia of the mental nerve or to avoid nerve damage during surgical procedures in the premolar area of the mandible. The present radiographic study aimed at evaluating the location and d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.07.033
更新日期：2013-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the tooth structure theoretically required to be removed in the coronal and cervical regions to accomplish 3 different endodontic access preparations in a novel digital model. The deviation of the center of access cavity from the central fossa will also be measured...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.01.002
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:This in vitro study compared cone-beam computed tomography (CBCT) exam with different voxel sizes with digital periapical radiography in the detection of vertical root fractures in teeth with and without intracanal metallic posts. METHODS:Eighteen single-rooted human teeth were endodontically treated, pre...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.031
更新日期：2013-12-01 00:00:00
abstract：:Intracoronal bleaching of pulpless teeth with 30% hydrogen peroxide may result in external cervical root resorption. One of several suggested etiological factors associated with this complication is the pH of the bleaching materials. Because the available data on the pH values of bleaching materials were conflicting, ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81988-6
更新日期：1991-08-01 00:00:00
abstract：INTRODUCTION:This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS:Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.011
更新日期：2017-10-01 00:00:00
abstract：INTRODUCTION:The anatomy of the maxillary C-shaped molar has been the subject of several case reports although no true prevalence research has ever been conducted. The aim of this observational study was to evaluate and characterize the incidence of these morphologies using cone-beam computed tomographic (CBCT) technol...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.12.013
更新日期：2016-03-01 00:00:00
abstract：:The aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: Facultative aerobes-anaerobes: Candida albicans ATCC 10231; Enterococcus faecalis ATCC 29212; Escherichia coli ATCC 25,922; Pseudomonas aeruginosa ATCC 27,85...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81170-2
更新日期：1999-05-01 00:00:00
abstract：:Root resorption after replantation of mature permanent dog incisors was examined by scanning electron microscopy. Three groups consisting of three extracted teeth each were kept in a saline solution for 5, 30, and 120 min before replantation. The first group was replanted with the pulp intact. The second and third gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80996-9
更新日期：1995-05-01 00:00:00
abstract：INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.011
更新日期：2014-02-01 00:00:00
abstract：INTRODUCTION:This randomized clinical trial assessed the effect of preoperative intraoral cryotherapy application on the success rate of inferior alveolar nerve blocks (IANBs) in patients with symptomatic irreversible pulpitis (SIP). METHODS:One hundred four patients with SIP were randomly distributed into 2 groups: c...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2019.05.001
更新日期：2019-08-01 00:00:00
abstract：INTRODUCTION:Warm vertically compacted gutta-percha obturation techniques use root canal sealers that are heated during the obturation. This study aims at investigating the suitability of selected sealers with warm gutta-percha obturation techniques. METHODS:The composition of an experimental sealer (Septodont; Saint ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.06.007
更新日期：2015-01-01 00:00:00
abstract：:The objective of this study was to assess and compare canal blockages and apical extrusion of dentin debris during canal shaping with eight preparation techniques. A total of 208 canals in extracted human teeth were prepared by one operator using one type of file. The techniques included were standardized, stepback wi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80443-7
更新日期：1995-03-01 00:00:00
abstract：:The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80217-2
更新日期：1996-07-01 00:00:00
abstract：INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.05.035
更新日期：2011-09-01 00:00:00
abstract：:Porphyromonas endodontalis (formerly Bacteroides endodontalis) is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. The presence of P. endodontalis in infected dental root can...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80843-5
更新日期：1992-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare, clinically and radiographically, the effects of platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA) as pulpotomy agents in permanent teeth with incomplete root development. METHODS:A total number of 70 children requiring pulpotomy in 70 pe...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2014.01.009
更新日期：2014-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in regulating tricalcium silicate (C3S)-driven proliferation and biomineralization of human dental pulp cells (hDPCs) in vitro. METHODS:Human DPCs were cultured in C3S-containing medium and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.03.002
更新日期：2013-08-01 00:00:00
abstract：INTRODUCTION:The introduction of piezoelectric instruments in endodontic surgery allowed clinicians to manage the bone tissue with precision reducing the risk of damaging soft tissues. Although such instruments can also be used to prepare root-end cavity, few information is available on the effect of piezosurgery on de...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.028
更新日期：2010-10-01 00:00:00
abstract：:This study was conducted to evaluate the influence of immediate versus delayed immersion time, and passive dye immersion versus centrifuged dye on apical leakage measurements. Eighty-four extracted human teeth with single straight canals were instrumented and divided into four experimental groups of 20 teeth each plus...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81364-6
更新日期：1993-07-01 00:00:00
abstract：:Cyanoacrylate is an extremely strong bonding agent which has many applications in medicine. It is also effective in obtaining a leakproof seal of rubber dam around a structurally compromised tooth requiring endodontic therapy. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81356-7
更新日期：1992-10-01 00:00:00
abstract：INTRODUCTION:This 2-part study hypothesized that a bioactive scaffold containing a sustained transforming growth factor (TGF)-β1-releasing nanoparticle system will promote migration and enhance differentiation of stem cells from the apical papilla (SCAP). The study aimed to develop and characterize a novel modified chi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.06.017
更新日期：2016-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this in-depth investigation was to identify, clarify, and substantiate clinical terminology relative to apical/periapical/periradicular diagnostic states, which is used routinely in the provision of endodontic care. Furthermore, the information gleaned from this investigation was used to lin...
journal_title：Journal of endodontics
pub_type： 共识发展会议,杂志文章
doi：10.1016/j.joen.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：:Silicone tubes filled with CRCS, a calcium hydroxide-based cement, were implanted at two different localizations in the right tibias of white male Wistar rats. A similar number of solid silicone rods were implanted in the left tibias of the same animals and were used as inert controls. Fifteen days after implantation ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80090-1
更新日期：1989-01-01 00:00:00
abstract：:This study attempts to evaluate the oral manifestations of and the limited available dental pulp information on diabetes mellitus, a common metabolic disorder of carbohydrate and lipid metabolism affecting over 16 million Americans. Diabetics are particularly prone to bacterial or opportunistic infections. This vulner...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1097/00004770-200306000-00001
更新日期：2003-06-01 00:00:00
