Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells.

Abstract:

INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Proliferation of the hDPCs was measured by methyl-thiazol-tetrazolium (MTT) assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction by using odontogenic marker genes such as dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP 1), osteocalcin (OC), alkaline phosphatase (ALP), and collagen type I (Col I), which were verified by ALP activity assessment, mineralization assay, and immunocytochemistry staining for dentin sialoprotein (DSP). RESULTS:The MTT assay showed that hDPCs cultured with Ca(3)SiO(5) extract proliferated more significantly as compared with Ca(OH)(2) extract. Analysis of odontogenic marker genes indicated that Ca(3)SiO(5) enhanced the expression of those genes. Moreover, the extract of Ca(3)SiO(5) stimulated mineralization and increased ALP and DSP production conspicuously. CONCLUSIONS:These results reveal that Ca(3)SiO(5) can induce the proliferation and odontogenic differentiation of hDPCs in vitro and might be a potential candidate for preparation of a new type of Ca(3)SiO(5-)based cement as a pulp-capping agent.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Peng W,Liu W,Zhai W,Jiang L,Li L,Chang J,Zhu Y

doi

10.1016/j.joen.2011.05.035

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

1240-6

issue

9

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(11)00666-2

journal_volume

37

pub_type

杂志文章
  • Nonsurgical root canal therapy of large cyst-like inflammatory periapical lesions and inflammatory apical cysts.

    abstract::It is a general belief that large cyst-like periapical lesions and apical true cysts caused by root canal infection are less likely to heal after nonsurgical root canal therapy. Nevertheless, there is no direct evidence to support this assumption. A large cyst-like periapical lesion or an apical true cyst is formed wi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,评审

    doi:10.1016/j.joen.2009.02.012

    authors: Lin LM,Ricucci D,Lin J,Rosenberg PA

    更新日期:2009-05-01 00:00:00

  • Autotransplantation of teeth with complete root formation: a case series.

    abstract:INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2010.04.028

    authors: Bae JH,Choi YH,Cho BH,Kim YK,Kim SG

    更新日期:2010-08-01 00:00:00

  • The Antimicrobial Peptide LL-37 as a Possible Adjunct for the Proliferation and Differentiation of Dental Pulp Stem Cells.

    abstract:INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, an...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.08.010

    authors: Milhan NVM,de Barros PP,de Lima Zutin EA,de Oliveira FE,Camargo CHR,Camargo SEA

    更新日期:2017-12-01 00:00:00

  • Glandular odontogenic cyst.

    abstract::This case report describes the clinical, radiologic, and histopathologic features of the glandular odontogenic cyst (GOC). Although a relatively rare lesion not previously reported in the endodontic literature, the GOC is recommended for inclusion in a differential diagnosis of a dentoalveolar radiolucency. The GOC is...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200403000-00014

    authors: Tran PT,Cunningham CJ,Baughman RA

    更新日期:2004-03-01 00:00:00

  • In vitro cytotoxicity evaluation of a self-adhesive, methacrylate resin-based root canal sealer.

    abstract::This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the resp...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2008.06.020

    authors: Pinna L,Brackett MG,Lockwood PE,Huffman BP,Mai S,Cotti E,Dettori C,Pashley DH,Tay FR

    更新日期:2008-09-01 00:00:00

  • Root Canal Treatment and Apical Periodontitis in a Brazilian Population with Type 1 Diabetes Mellitus: A Cross-sectional Paired Study.

    abstract:INTRODUCTION:This study radiographically analyzed the prevalence of root canal treatment (RCT) and apical periodontitis (AP) in type 1 diabetes mellitus (T1DM) and nondiabetic individuals and its association with the history/current status of T1DM. METHODS:In a cross-sectional paired study, the radiographic records of...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.02.010

    authors: Limeira FIR,Arantes DC,de Souza Oliveira C,de Melo DP,Magalhães CS,Bento PM

    更新日期:2020-06-01 00:00:00

  • Real-time polymerase chain reaction quantification of Porphyromonas gingivalis and Tannerella forsythia in primary endodontic infections.

    abstract:INTRODUCTION:Porphyromonas gingivalis and Tannerella forsythia are anaerobic bacteria commonly involved in root canal infections. Although previous investigations have assessed these species by strictly qualitative approaches, accurate determination of their cell levels by a sensitive quantitative technique may contrib...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2009.08.005

    authors: Saito D,Coutinho LL,Borges Saito CP,Tsai SM,Höfling JF,Gonçalves RB

    更新日期:2009-11-01 00:00:00

  • Glucose Transporter 2 and 4 Are Involved in Glucose Supply during Pulpal Wound Healing after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars.

    abstract:INTRODUCTION:Pulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via G...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.10.003

    authors: Tohma A,Ohkura N,Yoshiba K,Takeuchi R,Yoshiba N,Edanami N,Shirakashi M,Ibn Belal RS,Ohshima H,Noiri Y

    更新日期:2020-01-01 00:00:00

  • The number of bleaching sessions influences pulp tissue damage in rat teeth.

    abstract:INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.08.007

    authors: Cintra LT,Benetti F,da Silva Facundo AC,Ferreira LL,Gomes-Filho JE,Ervolino E,Rahal V,Briso AL

    更新日期:2013-12-01 00:00:00

  • Comparison of endodontist versus generalist regarding preference for postendodontic use of cotton pellets in pulp chamber.

    abstract::A degree of uncertainty exists about the use of cotton pellets in the pulp chamber after canal obturation. The purpose of this study was to poll practicing endodontists and generalists regarding their preference. Forty-six endodontists and an equal number of general dentists were polled in a mail survey regarding thei...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200209000-00007

    authors: Dillard CR,Barfield RD,Tilashalski KR,Chavers LS,Eleazer PD

    更新日期:2002-09-01 00:00:00

  • Investigation on the shaping ability of nickel-titanium files when used with a reciprocating motion.

    abstract:INTRODUCTION:The introduction of nickel-titanium (NiTi) files into clinical practice has improved the quality of canal shaping, but increasing the curvature of the root canal (or the diameter of the master instrument that prepares the full working length) could result in more transportation, straightening, and aberrati...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.06.030

    authors: Franco V,Fabiani C,Taschieri S,Malentacca A,Bortolin M,Del Fabbro M

    更新日期:2011-10-01 00:00:00

  • 3-Rooted Maxillary First Premolars: An Ex Vivo Study of External and Internal Morphologies.

    abstract:INTRODUCTION:This study aimed to analyze the external and internal morphologies of 3-rooted maxillary first premolars using cone-beam computed tomographic (CBCT) imaging. METHODS:Fifty-six three-rooted maxillary first premolars were imaged by CBCT imaging and classified into 4 groups on the basis of external root morp...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.03.045

    authors: Beltes P,Kalaitzoglou ME,Kantilieraki E,Beltes C,Angelopoulos C

    更新日期:2017-08-01 00:00:00

  • Characteristics of accessory mental foramina observed on limited cone-beam computed tomography images.

    abstract::In this retrospective study with limited cone-beam computed tomography (limited CBCT), we investigated the anatomic characteristics of the accessory mental foramina and accessory branches of the mandibular canal. The CBCT records of approximately 150 patients were evaluated, and 17 accessory mental foramina were found...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2008.08.033

    authors: Katakami K,Mishima A,Shiozaki K,Shimoda S,Hamada Y,Kobayashi K

    更新日期:2008-12-01 00:00:00

  • Descriptive Analysis of Factors Associated with External Cervical Resorption.

    abstract:INTRODUCTION:The aim of this study was to perform a descriptive analysis of the occurrence of external cervical resorption (ECR) in relation to the patients' characteristics (sex, age, and tooth type) and the potentially involved predisposing factors. METHODS:This study includes data on 284 patients (337 teeth with ev...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2017.05.026

    authors: Mavridou AM,Bergmans L,Barendregt D,Lambrechts P

    更新日期:2017-10-01 00:00:00

  • In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution.

    abstract::The aim of the present study was to evaluate the in vivo antimicrobial activity of 2% chlorhexidine gluconate (FCFRP-USP) used as a root canal irrigating solution in teeth with pulp necrosis and radiographically visible chronic periapical reactions. Culture techniques and measurement of the inhibition zone were used. ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(99)80135-6

    authors: Leonardo MR,Tanomaru Filho M,Silva LA,Nelson Filho P,Bonifácio KC,Ito IY

    更新日期:1999-03-01 00:00:00

  • Effectiveness of intracanal irrigants and medications against the yeast Candida albicans.

    abstract::An in vitro study was conducted to determine the susceptibility of the yeast Candida albicans to various intracanal irrigants and medications. The minimum inhibitory concentration (MIC) of sodium hypochlorite, hydrogen peroxide, chlorhexidine digluconate, and aqueous calcium hydroxide that is required to kill a standa...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200202000-00004

    authors: Ferguson JW,Hatton JF,Gillespie MJ

    更新日期:2002-02-01 00:00:00

  • Intracanal placement of Ca(OH)2: a comparison of techniques.

    abstract::Ten extracted human maxillary first molars were selected with a variety of root curvatures. The distofacial roots were resected and the mesiofacial canals were instrumented to a size #25 K file. Three techniques were used to introduce Ca(OH)2 paste into the instrumented canals. These techniques were evaluated for thei...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)81220-3

    authors: Sigurdsson A,Stancill R,Madison S

    更新日期:1992-08-01 00:00:00

  • Differential diagnoses for persistent pain after root canal treatment: a study in the National Dental Practice-based Research Network.

    abstract:INTRODUCTION:Pain present 6 months after root canal treatment (RCT) may be of odontogenic or nonodontogenic origin. This is important because treatments and prognoses are different; therefore, the aim of this study was to provide specific diagnoses of patients reporting pain 6 months after receiving initial orthograde ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2014.12.012

    authors: Nixdorf DR,Law AS,John MT,Sobieh RM,Kohli R,Nguyen RH,National Dental PBRN Collaborative Group.

    更新日期:2015-04-01 00:00:00

  • Effectiveness of ultrasonics and calcium hydroxide for the debridement of human mandibular molars.

    abstract::This study evaluated the ability of ultrasonics and calcium hydroxide to remove pulp tissue debris from the mesial root canals of human mandibular molars. All teeth were instrumented using a standard filing technique and irrigated with an equal volume of 2.6% sodium hypochlorite before the application of the experimen...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(89)80076-7

    authors: Metzler RS,Montgomery S

    更新日期:1989-08-01 00:00:00

  • A comparison of intracanal stresses in a post-restored tooth utilizing the finite element method.

    abstract::The finite element method was used to compare stresses along the inner canal wall in four two-dimensional models of an average maxillary central incisor. The four models evaluated were an intact incisor, an endodontically treated incisor, an endodontically treated crown-restored incisor, and a cylindrical post and cro...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81210-0

    authors: Cailleteau JG,Rieger MR,Akin JE

    更新日期:1992-11-01 00:00:00

  • Evaluation of a dentin barrier test by cyctotoxicity testing of various dental cements.

    abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(96)80285-8

    authors: Schmalz G,Schweikl H,Esch J,Hiller KA

    更新日期:1996-03-01 00:00:00

  • Risk assessment of the toxicity of solvents of gutta-percha used in endodontic retreatment.

    abstract::Three randomly assigned groups of single-canaled extracted teeth obturated with gutta-percha were retreated using controlled application of one of three organic solvents: chloroform, xylene, or halothane. Two additional groups of teeth served as positive and negative controls. Residual volume of solvent expressed thro...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80098-8

    authors: Chutich MJ,Kaminski EJ,Miller DA,Lautenschlager EP

    更新日期:1998-04-01 00:00:00

  • Root canal sealer cytotoxicity with human gingival Fibroblasts. III. Calcium hydroxide-based sealers.

    abstract::The cytotoxicity of four different calcium hydroxide-based root canal sealers (Sealapex, Apexit, CRCS, and Endoflas FS) was tested using human gingiva fibroblasts. The fibroblasts were acquired from the attached gingiva of apparently healthy patients. The sealers were allowed to set for 24 and 48 h before being covere...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81309-9

    authors: Briseño BM,Willershausen B

    更新日期:1992-03-01 00:00:00

  • Effect of T-cell deficiency on the formation of periapical lesions in mice: histological comparison between periapical lesion formation in BALB/c and BALB/c nu/nu mice.

    abstract::The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80565-0

    authors: Tani N,Kuchiba K,Osada T,Watanabe Y,Umemoto T

    更新日期:1995-04-01 00:00:00

  • Odontogenic cutaneous sinus tract.

    abstract::Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80959-3

    authors: Foster KH,Primack PD,Kulild JC

    更新日期:1992-06-01 00:00:00

  • Interleukin-1beta activity and collagen synthesis in human dental pulp fibroblasts.

    abstract::Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from h...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200203000-00003

    authors: Barkhordar RA,Ghani QP,Russell TR,Hussain MZ

    更新日期:2002-03-01 00:00:00

  • Effect of chlorhexidine and ethanol on the durability of the adhesion of the fiber post relined with resin composite to the root canal.

    abstract:INTRODUCTION:The aim of this study was to investigate the effects of pretreatment of gel chlorhexidine and ethanol on the bond strength and durability of the adhesion of the fiber post relined with resin composite to the root dentin. METHODS:Sixty bovine incisor roots were divided into four groups: irrigation with phy...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.01.007

    authors: Cecchin D,de Almeida JF,Gomes BP,Zaia AA,Ferraz CC

    更新日期:2011-05-01 00:00:00

  • Cytokine analysis in lesions refractory to endodontic treatment.

    abstract:INTRODUCTION:Failure in endodontic treatment is often caused by the persistence of microorganisms in the root canal after therapy. When treatment fails, an immune response develops that is characterized by an extensive network of immunologic mechanisms that lead to the production of cytokines and chemokines. METHODS:T...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.08.007

    authors: Henriques LC,de Brito LC,Tavares WL,Vieira LQ,Ribeiro Sobrinho AP

    更新日期:2011-12-01 00:00:00

  • Cytotoxicity against various cell lines of lipopolysaccharides purified from Bacteroides, Fusobacterium, and Veillonella isolated from infected root canals.

    abstract::The cytotoxicity against two mesenchymal cell lines, L-929 and WI-38, and two epithelial cell lines, KB and HeLa S-3, of lipopolysaccharide (LPS) purified from strains of Bacteroides gingivalis, Fusobacterium nucleatum, and Veillonella parvula isolated from infected root canals was investigated. The inhibition of cell...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(89)80195-5

    authors: Horiba N,Maekawa Y,Abe Y,Ito M,Matsumoto T,Nakamura H,Ozeki M

    更新日期:1989-11-01 00:00:00

  • Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics.

    abstract:INTRODUCTION:The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure. METHODS:Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the roo...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2016.04.029

    authors: Zeng Q,Nguyen S,Zhang H,Chebrolu HP,Alzebdeh D,Badi MA,Kim JR,Ling J,Yang M

    更新日期:2016-12-01 00:00:00