Abstract:
INTRODUCTION:The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment. METHODS:Conditioned media were prepared from 3 pulp capping biomaterials: Biodentine (Septodont, Saint-Maur-des-Fosses, France), TheraCal (BISCO, Lançon De Provence, France), and Xeno III (Dentsply Sirona, Versaille, France). Injured pulp fibroblasts were cultured with these conditioned media to analyze C5a secretion using an enzyme-linked immunosorbent assay. Dental pulp stem cells (DPSCs) were isolated from human third molar explants by magnetic cell sorting with STRO-1 antibodies. The expression of C5a receptor on DPSCs and inflammatory (THP-1) cells was investigated by immunofluorescence. The migration of both DPSCs and THP-1 cells was studied in Boyden chambers. RESULTS:Pulp fibroblast production of C5a significantly increased when the cells were incubated with TheraCal- and Xeno III-conditioned media. The recruitment of cells involved in inflammation (THP-1 cells) was significantly reduced by Biodentine- and TheraCal-conditioned media, whereas the migration of DPSCs was reduced with TheraCal- and Xeno III-conditioned media but not with that of Biodentine. The involvement of C5a in cell recruitment is demonstrated with a C5a receptor-specific antagonist (W54011). CONCLUSIONS:After pulp injury, the pulp capping material affects complement activation and the balance between inflammation and regeneration through a differential recruitment of DPSCs or inflammatory cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Giraud T,Rufas P,Chmilewsky F,Rombouts C,Dejou J,Jeanneau C,About Idoi
10.1016/j.joen.2017.02.016subject
Has Abstractpub_date
2017-07-01 00:00:00pages
1104-1110issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(17)30261-3journal_volume
43pub_type
杂志文章abstract::Locating the number and position of orifices on pulp-chamber floors can be difficult. This is especially true when the tooth being treated is heavily restored, malposed, or calcified. After evaluating 500 pulp chambers of extracted teeth, new laws for finding pulp chambers and root-canal orifices are proposed. The use...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200401000-00002
更新日期:2004-01-01 00:00:00
abstract::The effect of two different root canal cements and removal of the smear layer on the apical seal in roots obturated with Thermafil obturators was tested. Thirty extracted human mandibular first molars with 60 canals (mesial roots) were instrumented with the use of a step-back technique. Teeth were placed into 6 groups...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80131-3
更新日期:1998-05-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to determine factors and clinical situations that influence an endodontist's decision to use guided tissue regeneration (GTR) techniques during endodontic root-end surgery. METHODS:An invitation to participate in a web-based survey was e-mailed to 3,750 members of the America...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.017
更新日期:2011-11-01 00:00:00
abstract::This report presents an unusual indication for root resection of a lower molar tooth and also reviews the history of root resection. ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1097/00004770-200004000-00016
更新日期:2000-04-01 00:00:00
abstract:INTRODUCTION:Proteinases play pivotal roles in Candida albicans infections. Although the yeast can colonize the pulpal environment, there is no information about the enzymatic profile of this organism. This in vitro study aimed to determine the proteolysis levels and to investigate differences in the expression of aspa...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.01.012
更新日期:2015-06-01 00:00:00
abstract:INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.035
更新日期:2011-09-01 00:00:00
abstract::The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 x 1 mm). For cytotoxicity testing (MTT method), the spe...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.05.006
更新日期:2006-10-01 00:00:00
abstract:INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the prolifera...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.01.038
更新日期:2015-06-01 00:00:00
abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80460-7
更新日期:1993-06-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to examine the size, volume, and other parameters of preoperative periapical lesions measured from cone-beam computed tomographic (CBCT) images as potential prognostic factors in endodontic microsurgery. METHODS:A clinical database was searched for patients who had received e...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.006
更新日期:2016-08-01 00:00:00
abstract::The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited ca...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000155259.22746.ae
更新日期:2005-09-01 00:00:00
abstract::The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO prod...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.07.012
更新日期:2007-11-01 00:00:00
abstract::Little is known about the effect of aging on characteristic functions of pulp cells. When damaged pulp is recovered and mineralized tissue is formed to protect remaining pulp tissue, the general responses of pulp tissue after adequate stimuli (pulp cell proliferation and activation of alkaline phosphatase [ALPase]) ar...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200301000-00003
更新日期:2003-01-01 00:00:00
abstract::There have been major recent reorganizations among bacterial taxa as a result of phylogenetic taxonomic approaches. As a consequence, old species have been renamed and novel species have been proposed. The introduction of molecular technology for microbial identification has also allowed the detection of microbial tax...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1097/00004770-200310000-00001
更新日期:2003-10-01 00:00:00
abstract::To date there has been very little data on the cytotoxic response of different cell lines to root canal sealers. The objective of this study was to determine the cytocompatibility of three different extracts of root canal sealers and to compare the cytotoxic response of these materials on two different primary human o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200109000-00004
更新日期:2001-09-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to investigate the expression of matrix metalloproteinases (MMPs) in apical periodontitis and during the periapical healing phase after root canal treatment. METHODS:Apical periodontitis was induced in dog teeth, and root canal treatment was performed in a single visit or b...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.10.030
更新日期:2010-02-01 00:00:00
abstract:INTRODUCTION:This study evaluated the influence of cervical preflaring on the incidence of root dentin defects after root canal preparation. METHODS:Extracted human maxillary central incisors were selected and allocated to 1 control group and 12 experimental groups (n = 15). Teeth in the control group were left unprep...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.021
更新日期:2018-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of various degrees of cyclic fatigue on torsional failure and torsional preloading on the cyclic fatigue life of heat-treated K3XF nickel-titanium (NiTi) instruments (SybronEndo, Orange, CA). METHODS:The mean number of cycles until failure (Nf) of K3XF ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.10.008
更新日期:2015-04-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to visualize the stresses and strain distribution patterns in ProTaper Universal F2 files (Dentsply Maillefer, Ballaigues, Switzerland) and to establish the stress- and strain-curvature relationship for this instrument under various conditions by using a dynamic, three-dimensi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.06.003
更新日期:2011-10-01 00:00:00
abstract::This case report describes an innovative virtual simulation method using a computer-aided rapid prototyping (CARP) model and a computer-aided design (CAD) program for autotransplantation of an immature third molar.A compromised left mandibular second molar (#18) was extracted and replaced by autotransplantation using ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.08.010
更新日期:2018-12-01 00:00:00
abstract::Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80959-3
更新日期:1992-06-01 00:00:00
abstract::This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80158-X
更新日期:1989-12-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to examine the level of erosion in root dentin caused by different irrigation methods and protocols. METHODS:Thirty-five extracted upper molar teeth were instrumented and divided into 7 groups to undergo treatment by different methods: negative control, GentleWave System (Son...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.07.024
更新日期:2016-12-01 00:00:00
abstract::The difference in resistance to bacterial invasion into the dentinal tubules between vital and nonvital teeth has not been determined. This study was conducted to clarify the effect of vital pulp on bacterial invasion into the dentinal tubules. The specimens were 19 intact pairs of bilateral upper third molars of 19 h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81098-8
更新日期:1995-02-01 00:00:00
abstract::A comparison of the microleakage of mineral trioxide aggregate (MTA) and a high copper admix amalgam (Valiant PhD) in root-end preparations was made using a fluid conductive device. Thirty-three bilaterally matched pairs of extracted, single-rooted teeth were prepared and obturated using lateral compaction of gutta-pe...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80160-X
更新日期:1998-11-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate the mineral contents of root-canal dentin before and after treatment with two commonly used gutta-percha solvents: chloroform and halothane. Twenty extracted human premolars, whose crowns and apical thirds had been removed, were used. Pulp tissues were removed and the...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200401000-00012
更新日期:2004-01-01 00:00:00
abstract::A new endodontic obturation and retrofilling material has been invented to satisfy Grossman's requirements for an ideal material. Methylene blue dye leakage studies were carried out to compare the new material with conventional products, for both obturation and retrofilling. The experimental material when used in root...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200207000-00008
更新日期:2002-07-01 00:00:00
abstract:INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.015
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.02.020
更新日期:2015-07-01 00:00:00
abstract:INTRODUCTION:Microorganisms are able to survive and cause persistent infection in the extraradicular area. The aims of this study were to investigate the primary bacterial flora and the localization of extraradicular biofilm in persistent apical periodontitis lesions. METHODS:Apical root samples from root-end surgery ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.03.004
更新日期:2012-07-01 00:00:00