Abstract:
INTRODUCTION:Dentin regeneration is one of the main goals of vital pulp treatment in which the biological properties of dental pulp cells (DPCs) need to be considered. In our previous study, we showed that EDTA could enhance the stromal cell-derived factor 1 alpha-induced migration of DPCs. The purpose of this study was to explore the effects of EDTA on the mineralization of dental pulp in vitro and in vivo. METHODS:DPCs were obtained from human premolars or third molars. Alkaline phosphatase assays and alizarin red S staining were used to examine the degree of differentiation and mineralized nodule formation of DPCs. Real-time polymerase chain reaction and Western blot analysis were performed to detect the messenger RNA and protein expressions of mineralization-related markers in DPCs. Extracellular-regulated protein kinase and Smad inhibitors were used to study the roles of these 2 signaling pathways in this process. In addition, pulp exposures were created on 18 premolars of 2 beagle dogs (>12 months) using a high-speed dental handpiece. The experimental group (n = 9) was treated with 12% EDTA for 5 minutes, and the control group (n = 9) was treated with sterile saline for the same duration. Mineral trioxide aggregate was used for direct pulp capping followed by glass ionomer cement sealing. Samples were collected 3 months later, and the regenerated dentin was assessed by micro-computed tomographic and histologic analyses. RESULTS:Exposure to 12% EDTA promoted the activity of alkaline phosphatase, the formation of mineralized nodules, and the messenger RNA and protein expressions of mineralization-related markers in DPCs. Furthermore, the process of 12% EDTA enhancing the differentiation of DPCs was mediated by the extracellular-regulated protein kinase 1/2 signaling pathway and inhibited by the Smad2/3 signaling pathway. In vivo, compared with the control group, more regenerated dentin that had fewer tunnel defects was formed in the 12% EDTA-treated group. CONCLUSIONS:Our results showed that 12% EDTA could promote the mineralization of dental pulp in vitro and in vivo.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Liu L,Leng S,Tang L,Lu Q,Xu W,Tan X,Huang D,Zhang Ldoi
10.1016/j.joen.2020.12.003subject
Has Abstractpub_date
2020-12-20 00:00:00eissn
0099-2399issn
1878-3554pii
S0099-2399(20)30958-4pub_type
杂志文章abstract::Historically, pulp-necrotizing agents were commonly used in endodontic treatments. They act quickly and devitalize the pulp within a few days. However, they are cytotoxic to gingiva and bone. If such an agent diffuses out of the cavity, it can readily cause widespread necrosis of gingiva and bone, which can lead to os...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000125881.18872.ef
更新日期:2004-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this article was to compare the cyclic fatigue resistance of Reciproc and Reciproc Blue files (VDW GmbH, Munich, Germany) that were used to prepare root canals of mandibular molar teeth with or without a glide path. METHODS:Sixty Reciproc R25 and 60 Reciproc Blue R25 files were used. The Re...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.03.004
更新日期:2018-06-01 00:00:00
abstract:INTRODUCTION:This study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (MTA) mixed with aqueous and propylene glycol extracts of Arctium lappa L. and Casearia sylvestris Sw. METHODS:The setting-time test was performed according to American Society for Testin...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.12.023
更新日期:2017-05-01 00:00:00
abstract:INTRODUCTION:An ideal orthograde or retrograde filling material should seal the pathways of communication between the root canal system and its surrounding tissues. It should also be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable. Mineral trioxide aggregate ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.09.006
更新日期:2010-01-01 00:00:00
abstract:INTRODUCTION:It was recently reported that heme oxygenase-1 (HO-1) activity is related to stem cell differentiation; however, the involvement of HO-1 in pulp cell growth and differentiation has not been well explored. The purpose of this study was to investigate the role of HO-1 in the growth and differentiation of hum...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.04.011
更新日期:2010-08-01 00:00:00
abstract:INTRODUCTION:Neuropeptides play an important role in inflammation and repair and have been implicated in mediating angiogenesis. Pulp fibroblasts express neuropeptide receptors, and the aim of this research was to investigate whether neuropeptides could regulate angiogenic growth factor expression in vitro METHODS:An ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.03.005
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the cell viability of dental pulp cells treated with Biodentine (Septodont, Saint-Maur, France) and mineral trioxide aggregate (MTA) and the in vitro and in vivo expression of mineralization markers induced by the 2 materials. METHODS:Human dental pulp cells isolat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.12.018
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of low-surface tension EDTA solutions on the push-out bond strength of resin-based sealer to young and old root canal dentin. METHODS:Root canals from 64 (n = 32 age <30 years old and n = 32 >60 years old) extracted, decoronized, single-rooted human tee...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.007
更新日期:2018-03-01 00:00:00
abstract::Biopsy reports of specimens from the apices of 1659 teeth physically present at the time the biopsy was performed were reviewed. The following information was recorded from each report: (a) source of specimen, (b) sex of patient, (c) age of patient, (d) location of tooth associated with lesion, and (e) diagnoses repor...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/s0099-2399(06)81679-1
更新日期:1990-05-01 00:00:00
abstract:INTRODUCTION:Microorganisms are able to survive and cause persistent infection in the extraradicular area. The aims of this study were to investigate the primary bacterial flora and the localization of extraradicular biofilm in persistent apical periodontitis lesions. METHODS:Apical root samples from root-end surgery ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.03.004
更新日期:2012-07-01 00:00:00
abstract:INTRODUCTION:The dental pulp is highly vascularized and innervated tissue that is uniquely designed, being highly biologically active, while being enclosed within the calcified structure of the tooth. It is well-established that the dental pulp vasculature is a key requirement for the functional performance of the toot...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.06.033
更新日期:2020-09-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the efficacy of the cutting ability of two different instruments, concerning changes in the dentin thickness removed and root canal volume, by using multislice computed tomography scanning. METHODS:Thirty single-rooted mandibular premolars were divided into two equ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.03.036
更新日期:2011-08-01 00:00:00
abstract::The aim of this laboratory based study was to determine the shaping ability of Lightspeed nickel-titanium rotary instruments in simulated root canals. A total of 40 canals with four different shapes in terms of angle and position of curve were prepared with Lightspeed instruments using the stepback technique recommend...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80347-0
更新日期:1997-12-01 00:00:00
abstract:INTRODUCTION:Studies have found women try to avoid pain more than men, accept it less, and fear it more. The role of operator gender on subject gender pain in a clinical setting needs further study. The purpose of this randomized, double-blind investigation was to evaluate operator gender and its influence on reported ...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2014.10.001
更新日期:2015-02-01 00:00:00
abstract::The 4- to 6-yr outcome of initial (first-time) endodontic treatment was assessed for Phase II of the "Toronto Study." In total, 442 teeth were treated by using flared preparation and vertical compaction of warm gutta-percha or step-back preparation and lateral compaction. With 126 teeth excluded (discontinuers: deceas...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200405000-00002
更新日期:2004-05-01 00:00:00
abstract:INTRODUCTION:Various materials have been advocated for use as root-end filling materials. The purpose of the present in vitro study was to compare the cytotoxicity of 4 root-end filling materials: glass ionomer cement (GIC; Fuji II, GC Corp, Tokyo, Japan), reinforced zinc oxide-eugenol cement (IRM; Dentsply Tulsa Denta...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.09.004
更新日期:2012-12-01 00:00:00
abstract:INTRODUCTION:This study was conducted to evaluate and compare the fracture resistance of roots obturated with various contemporary canal-filling systems. METHODS:Fifty single-rooted extracted mandibular premolars were decoronated to obtain 13-mm root segments. Ten roots were left unprepared and unfilled (negative cont...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.08.008
更新日期:2011-11-01 00:00:00
abstract::Ocular complications after an inferior alveolar nerve anesthesia are rare. These complications, although temporary and benign, can be distressing to both the patient and the clinician. A 37-year-old male patient was administered an inferior alveolar nerve block for the root canal treatment of tooth #30. Immediately af...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2018.05.005
更新日期:2018-09-01 00:00:00
abstract:INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.028
更新日期:2014-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to investigate the effects of instrumentation and irrigation on the initial adherence of Enterococcus faecalis to root canal dentin and to explore initial microbial adhesion to root filling materials. METHODS:The following specimens were prepared: instrumented and uninstrumen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.04.006
更新日期:2019-07-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to examine the size, volume, and other parameters of preoperative periapical lesions measured from cone-beam computed tomographic (CBCT) images as potential prognostic factors in endodontic microsurgery. METHODS:A clinical database was searched for patients who had received e...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.006
更新日期:2016-08-01 00:00:00
abstract::This study is a quantitative assessment of the luminous energy transmitted through different translucent fiber posts. After embedding the posts in black resin, the blocks were submitted to sequential cuts in a precision machine, and depths of 16 mm, 12 mm, 8 mm, and 4 mm were assessed for light transmission with a dig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.12.007
更新日期:2008-03-01 00:00:00
abstract:INTRODUCTION:Achieving anesthesia in mandibular molar teeth with irreversible pulpitis is very difficult. The aim of this study was to compare the efficacy of 1.8 mL and 3.6 mL articaine for an inferior alveolar nerve block (IANB) when treating molars with symptomatic irreversible pulpitis. METHODS:In a randomized, do...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.05.015
更新日期:2015-09-01 00:00:00
abstract::Whole genome DNA-DNA hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. There are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-re...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200407000-00014
更新日期:2004-07-01 00:00:00
abstract::The sealing properties of three temporary restorative materials, Cavit, IRM, and a polycarboxylate-based cement, Ultratemp Firm, were investigated in vitro. Standardized access cavities were prepared in 45, intact, extracted, human molars. The teeth were randomly assigned to three groups and the access openings filled...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.don.0000121610.63000.f2
更新日期:2004-08-01 00:00:00
abstract::A method is proposed for a three-dimensional visualization of the root canal system before and after the cleaning and shaping procedure to analyze the relationships of the root canal walls. The first part of the study provided cross-sections of the root perpendicular to the root canal. A system for remounting the sect...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81298-7
更新日期:1993-05-01 00:00:00
abstract::Bisphosphonates are commonly used in medicine to maintain bone density in patients with certain nonneoplastic diseases or cancers. A serious adverse effect of bisphosphonates that has substantial dental significance is osteonecrosis that appears to uniquely affect the mandible and maxilla without occurring in other bo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000186481.96169.cd
更新日期:2005-11-01 00:00:00
abstract::This case series reports the outcomes of 8 patients (ages 9-14 years) who presented with 9 immature permanent teeth with pulpal necrosis and apical periodontitis. During treatment, 5 of the teeth were found to have at least some residual vital tissue remaining in the root canal systems. After NaOCl irrigation and medi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.03.023
更新日期:2008-07-01 00:00:00
abstract:INTRODUCTION:This in vitro study compared the cyclic fatigue resistance of ProFile Vortex rotary instruments (DENTSPLY Tulsa Dental, Tulsa, OK) made of two different raw materials: M-Wire and regular superelastic wire (SE-wire) at two different rotational speeds. Two rotary-based Profile Vortex instruments with taper 0...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.02.015
更新日期:2010-07-01 00:00:00
abstract:INTRODUCTION:This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. METHODS:The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Prolif...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.035
更新日期:2011-09-01 00:00:00