Abstract：
INTRODUCTION:Microorganisms are able to survive and cause persistent infection in the extraradicular area. The aims of this study were to investigate the primary bacterial flora and the localization of extraradicular biofilm in persistent apical periodontitis lesions. METHODS:Apical root samples from root-end surgery were collected from 23 root-filled teeth with apical periodontitis. Five samples were examined for the presence of biofilm by scanning electron microscopy. Another 5 samples were examined for the presence of biofilm by Brown and Brenn-modified Gram staining. The DNA from 13 samples was processed for amplification via polymerase chain reaction and separated with denaturing gradient gel electrophoresis. Selected bands were excised from the gel and sequenced for identification. RESULTS:The extraradicular biofilm present on the external root surface of treated teeth consisted of abundant, amorphous extracellular material and multiple bacterial species. The following species were detected in the microbial community from the apical samples: Actinomyces sp. oral, Propionibacterium, Prevotella sp. oral, Streptococcus, Porphyromonas endodontalis, and Burkholderia. The prevalence of Actinomyces sp. oral and Propionibacterium were highest (84.6% and 61.5%, respectively). CONCLUSIONS:Extraradicular biofilm was present on the external root surface of treated teeth with persistent periapical lesions. Actinomyces sp. oral and Propionibacterium are likely important contributors to extraradicular biofilm formation and persistent periapical infection.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wang J,Jiang Y,Chen W,Zhu C,Liang Jdoi
10.1016/j.joen.2012.03.004subject
Has Abstractpub_date
2012-07-01 00:00:00pages
954-9issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(12)00285-3journal_volume
38pub_type
杂志文章abstract：:The purpose of this study was to compare clinical, radiographic, and histopathologic effects of Nd:YAG laser pulpotomy to formocresol pulpotomy on human primary teeth. Patients with at least two vital primary molar teeth that required pulpotomy, because of pulpal exposure to caries, were selected for this study. After...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.12.013
更新日期：2007-04-01 00:00:00
abstract：:The anatomy of third molars has been described as unpredictable. However restorative, prosthetic, and orthodontic considerations often require endodontic treatment of third molars in order for them to be retained as functional components of the dental arch. The purpose of this study was to investigate and characterize...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200011000-00011
更新日期：2000-11-01 00:00:00
abstract：:The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.11.005
更新日期：2007-01-01 00:00:00
abstract：:In the present study, we used Fos expression as an index of nociceptive input to the spinal trigeminal nucleus after exposure of the coronal pulp tissue of maxillary right first molars and examined the effects of pretreatment with an opioid, a nonsteroidal anti-inflammatory drug or a local anesthetic before pulp expos...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000153844.61709.64
更新日期：2005-08-01 00:00:00
abstract：:Advanced glycation end products (AGEs) have been found to play a role in inflammation and vascular complications. The receptor for AGE (RAGE) is expressed on many cells and is up-regulated during inflammation. To date, RAGE expression or its correlation with nuclear factor kappa beta (NF-kappabeta) expression has not ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.02.013
更新日期：2008-06-01 00:00:00
abstract：:Because of its close proximity to teeth and periodontium, gingival squamous cell carcinoma (SCC) can sometimes mimic tooth-related benign inflammatory conditions, resulting in misdiagnosis. In this study we report a case of gingival SCC that mimicked a dentoalveolar abscess of endodontic origin in its early presentati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.08.005
更新日期：2007-02-01 00:00:00
abstract：:Prostaglandins (PG) have been implicated in the genesis of periapical lesions. Periapical specimens from patients with clinical signs and symptoms of chronic and acute apical periodontitis were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars were frozen and used as...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81737-1
更新日期：1991-03-01 00:00:00
abstract：:EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200112000-00007
更新日期：2001-12-01 00:00:00
abstract：:In this retrospective study with limited cone-beam computed tomography (limited CBCT), we investigated the anatomic characteristics of the accessory mental foramina and accessory branches of the mandibular canal. The CBCT records of approximately 150 patients were evaluated, and 17 accessory mental foramina were found...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.033
更新日期：2008-12-01 00:00:00
abstract：:Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000164855.98346.fc
更新日期：2005-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the tooth structure theoretically required to be removed in the coronal and cervical regions to accomplish 3 different endodontic access preparations in a novel digital model. The deviation of the center of access cavity from the central fossa will also be measured...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.01.002
更新日期：2020-04-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to analyze and characterize root canal morphology of mandibular molars of the Brazilian population by using cone-beam computed tomography (CBCT). METHODS:Patients who required CBCT radiographic examinations as part of their routine examination, diagnosis, and treatment planning w...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.030
更新日期：2013-07-01 00:00:00
abstract：:Aspirating the unknown contents of a bony cavity and saline irrigation of a body wound are both basic surgical techniques. These two techniques have been combined in treating persistent periradicular pathosis in two cases. The cases demonstrate significant bony healing of extensive periradicular defects after the use ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81968-0
更新日期：1990-04-01 00:00:00
abstract：:The aim of this in vitro study was to investigate the effect of the smear layer on apical microleakage in teeth obturated with mineral trioxide aggregate (MTA). Fifty single-rooted central maxillary teeth were used in this study. All teeth were instrumented to size 60 by using the step-back technique. The selected tee...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2008.08.022
更新日期：2008-12-01 00:00:00
abstract：INTRODUCTION:Dens invaginatus is a critical condition for endodontic treatment once it frequently presents a complex internal anatomy and might be associated with incomplete root and apical development. METHODS:Three cases of dens invaginatus with open apex in maxillary lateral incisors are presented. In the first cas...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2010.02.002
更新日期：2010-06-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure. METHODS:Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the roo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.04.029
更新日期：2016-12-01 00:00:00
abstract：:A case is presented in which endodontic treatment of a maxillary lateral incisor with a talon cusp and two root canals failed following a mistaken diagnosis. What was first diagnosed as an endodontic lesion was, in all probability, a primary periodontal lesion caused by the advance of bacteria from the gingival crevic...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81947-3
更新日期：1990-07-01 00:00:00
abstract：INTRODUCTION:The purpose of this prospective, randomized, single-blinded study was to measure the degree of anesthesia obtained with 1.8 mL and 3.6 mL of 2% lidocaine with 1:100,000 epinephrine in posterior superior alveolar (PSA) nerve blocks. METHODS:Thirty-one adult subjects randomly received PSA nerve blocks of 1....
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2010.01.009
更新日期：2010-04-01 00:00:00
abstract：:The purpose of this study was to compare the cleaning efficacy of passive ultrasonic activation with that of passive sonic activation after hand instrumentation. Sixty curved molar canals were hand-instrumented to size 35 and divided into three groups. Group 1 received no further treatment. Group 2 received 3 min of p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80120-4
更新日期：1999-11-01 00:00:00
abstract：:The sealing ability of copal varnish has been investigated when used as an adjunct in restorative procedures, retrofilling procedures, and dontic obturation procedures. Barrier, a polyamide-type polymer cavity varnish, has been compared with copal varnishes in restorative dentistry. This investigation compared the api...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81138-6
更新日期：1992-01-01 00:00:00
abstract：INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.02.028
更新日期：2014-10-01 00:00:00
abstract：:The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80217-2
更新日期：1996-07-01 00:00:00
abstract：:The purpose of this study was to compare three engine driven (nickel-titanium) NiTi instrument systems with hand files for their effect on canal transportation. Mesial roots of mature lower first molars with separate canals were paired on the basis of curvature and morphology. Canal lengths were standardized to 11 mm ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80310-X
更新日期：1997-08-01 00:00:00
abstract：INTRODUCTION:The aim was to assess the association of inducible costimulator (ICOS) and ICOS ligand with bone destruction in apical periodontitis (AP). METHODS:Specimens from patients presenting with AP were obtained during apicoectomy and subjected to histopathologic analysis and molecular assessment of ICOS/ICOS lig...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.018
更新日期：2019-07-01 00:00:00
abstract：INTRODUCTION:This study assessed apical extrusion during treatment with GentleWave (GW; Sonendo Inc, Laguna Hills, CA), a conventional open-ended 30-G needle (CN), or Endovac (EV; SybronEndo, Orange, CA) in root canals enlarged to different dimensions with and without apical constriction. METHODS:Sixteen mandibular mo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.04.009
更新日期：2016-01-01 00:00:00
abstract：:A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.11.004
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:This study reports the antimicrobial effect of photodynamic therapy (PDT) combined with endodontic treatment in patients with necrotic pulp infected with microflora resistant to a previous antibiotic therapy. METHODS:Thirty anterior teeth from 21 patients with periapical lesions that had been treated with...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章
doi：10.1016/j.joen.2010.06.001
更新日期：2010-09-01 00:00:00
abstract：INTRODUCTION:The aim of this prospective study was to evaluate the postoperative pain that followed root canal treatments performed with a single-file reciprocating system on asymptomatic uniradicular necrotic teeth with and without foraminal enlargements (FEs). METHODS:Forty-six volunteers were randomly divided into ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2015.09.018
更新日期：2016-01-01 00:00:00
abstract：INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to examine the size, volume, and other parameters of preoperative periapical lesions measured from cone-beam computed tomographic (CBCT) images as potential prognostic factors in endodontic microsurgery. METHODS:A clinical database was searched for patients who had received e...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.05.006
更新日期：2016-08-01 00:00:00
