Abstract：
:EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibroblasts using Trypan blue. The solutions were diluted to 1%, 0.1%, and 0.01% and applied to NIH 3T3 cell cultures. Cells grown on fresh DMEM served as a control. After 0, 6, 12, and 24 h (short-term assay, viability) and 1, 3, 5, and 7 days (long-term assay, survival), the cells were counted using a hemocytometer. In short-term tests, cell viability ranged from 85% to 99% for all experimental groups with no statistical differences when compared with control cultures, except for the group treated with 1% EDTA-T, which caused a progressive decrease in cell viability. In long-term tests, all cultures increased in number from day 1 to the end of the experimental period, showing no inhibition of cell proliferation, except for the cultures treated with 1% EDTA-T, which totally prevented cell growth. All dilutions of 10% citric acid were more biocompatible than EDTA-T. Cultures treated with citric acid had a higher percentage of viable cells in the short-term assays, and the cells retained their self-renewal capacity.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Sceiza MF,Daniel RL,Santos EM,Jaeger MMdoi
10.1097/00004770-200112000-00007keywords:
subject
Has Abstractpub_date
2001-12-01 00:00:00pages
741-3issue
12eissn
0099-2399issn
1878-3554pii
S0099-2399(05)61031-Xjournal_volume
27pub_type
杂志文章abstract：INTRODUCTION:The purpose of this study was to evaluate the antimicrobial effects of root canal sealers on Enterococcus faecalis biofilms in dentinal tubules by using a novel dentin infection model. METHODS:Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart inf...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.10.042
更新日期：2014-04-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63). METHODS:A mitochondrial colorimetric assay was used to evaluate the MG63 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.03.039
更新日期：2010-07-01 00:00:00
abstract：:The aims of this study were to compare Resilon (Resilon Research LLC, Madison, CT) in conjunction with either a bonding (Epiphany; Pentron Clinical Technologies, Wallingford, CT) or a nonbonding endodontic sealer (Pulp Canal Sealer; Kerr Corporation, Orange, CA) to EndoRez (Ultradent Products Inc, South Jordan, UT) an...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.02.018
更新日期：2007-07-01 00:00:00
abstract：:The aim of this study was to measure root-dentin microhardness after instrumentation with two types of files and using irrigation with 2.5% sodium hypochlorite. Thirty roots were instrumented with irrigation: 10 roots had the pulp extirpated only; 10 roots were instrumented with stainless steel files, and 10 roots wer...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200204000-00012
更新日期：2002-04-01 00:00:00
abstract：:Unlike most cases of combined orthodontic and endodontic therapy that have been reported, in this case endodontic treatment with calcium hydroxide-induced calcification was utilized prior to orthodontic treatment. A 14-yr-old female required orthodontic therapy due to anterior crowding. Preoperative periapical radiogr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80290-6
更新日期：1994-05-01 00:00:00
abstract：INTRODUCTION:The introduction of nickel-titanium (NiTi) files into clinical practice has improved the quality of canal shaping, but increasing the curvature of the root canal (or the diameter of the master instrument that prepares the full working length) could result in more transportation, straightening, and aberrati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.06.030
更新日期：2011-10-01 00:00:00
abstract：:Amalgam cores with cemented dowels are commonly used to restore endodontically treated teeth. These restorations were widely studied for their mechanical properties; however, less is known about their sealing ability. The effect of the cement type used for the dowel and that of a bonding agent application were studied...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200104000-00015
更新日期：2001-04-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) has been used successfully for perforation repair, vital pulpotomies, and direct pulp capping. However, little is known about the interactions between MTA and glass ionomer cement (GIC) in final restorations. In this study, 2 null hypotheses were tested: (1) GIC placement t...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2012.04.013
更新日期：2012-08-01 00:00:00
abstract：:The aim of this study was to determine the location of the root canal curvature and measure the distance from the CEJ to the first curvature using in vitro methods. Extracted maxillary premolars (n = 358) were fixed and digitally radiographed using the parallel technique. Excluded from further analysis were teeth with...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.09.011
更新日期：2006-04-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to introduce an innovative method for autotransplantation of teeth using 3-dimensional (3D) surgical templates for guided osteotomy preparation and donor tooth placement. METHODS:This report describes autotransplantation of immature premolars as treatment of an 11-year-old boy ha...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.08.021
更新日期：2016-12-01 00:00:00
abstract：INTRODUCTION:This study addressed the following population, intervention, comparator, outcome, timing, study design and setting question: in patients with preoperative pain who undergo single-visit nonsurgical endodontic treatment, what is the comparative efficacy of corticosteroids compared with other analgesics or pl...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2018.05.015
更新日期：2018-09-01 00:00:00
abstract：INTRODUCTION:Cell migration is an important step in pulpal wound healing. Although components in the resin-based dental materials are known to have adverse effects on pulp wound healing including proliferation and mineralization, their effects on cell migration have been scarcely examined. Here, we investigated the eff...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.004
更新日期：2013-09-01 00:00:00
abstract：:The purpose of this study was to quantify the amount of remaining gutta-percha/sealer after retreatment of post space prepared teeth obturated with a lateral condensation technique or with Thermafil with plastic and metallic carrier. The time required for retreatment was also recorded. Forty-five extracted mandibular ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80019-1
更新日期：1994-01-01 00:00:00
abstract：:Immune and nervous systems play key roles in periapical pathosis; however, their spatial associations, which may be a prerequisite for paracrine interactions in the progression of periapical lesions, remain to be examined. In this study we examined the distribution and frequency of spatial associations between major h...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.08.014
更新日期：2007-02-01 00:00:00
abstract：INTRODUCTION:Information on the nerve fibers innervating the dental pulp is crucial for understanding dental pain and hypersensitivity. This study investigated the morphologic differences of parvalbumin (PV)-positive (+) myelinated fibers in 3 different regions of the human dental pulp. METHODS:Light and electron micr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.01.010
更新日期：2017-06-01 00:00:00
abstract：INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesize...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.015
更新日期：2019-01-01 00:00:00
abstract：:Orthodontic tooth movement has been implicated in secondary changes to the dental pulp. The purpose of this study was to correlate the effects of orthodontic tooth movement on the dental pulp by histomorphometric parameters. Four groups, each consisting of 36 male adult Sprague-Dawley strain rats, were studied with di...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81034-4
更新日期：1993-01-01 00:00:00
abstract：:Many root canal instruments with several different designs have been introduced during recent years. This article evaluates the machining properties of four such instruments: the Flex-R, K-Flex, Trio-Cut, and S file. There are large variations in the machining properties of these instruments. The results are comparabl...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(07)80200-7
更新日期：1990-12-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：:Type IV matrix metalloproteinases (MMPs) are members of the family of MMPs and are thought to play an important role in degradation of extracellular components. Human pulp cells can secrete and produce these enzymes. Recent evidence shows that MMPs may play a role in pulpal inflammation. To date little is known regard...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200111000-00007
更新日期：2001-11-01 00:00:00
abstract：:This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.016
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effects of endodontic irrigants on the push-out strength and hydration behavior of accelerated mineral trioxide aggregate (MTA) in its early setting phase. METHODS:In an in vitro perforation model, MTA with or without 10% CaCl(2) was condensed and allowed to i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.039
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the fracture strength of roots instrumented with the Self-Adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) and the ProTaper system (Dentsply Maillefer, Ballaigues, Switzerland) and filled with the cold lateral compaction technique. METHODS:In total, 50 mandibul...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.08.030
更新日期：2014-04-01 00:00:00
abstract：:This study aimed to investigate periapical exudate neutrophil elastase (NE) and prostaglandin E2 (PGE2) levels and their relationships with clinical symptoms, and to determine the changes of their levels following first treatment visit. Periapical exudate samples were collected from the canals of 34 nonvital single-ro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000158010.43884.59
更新日期：2005-11-01 00:00:00
abstract：INTRODUCTION:Krüppel-like factor 4 (KLF4) plays an important role in cytodifferentiation and proliferation. Our previous study showed that KLF4 was specifically expressed in polarizing and elongating odontoblasts. However, the role of KLF4 in odontoblast differentiation was still unknown. The purpose of this study was ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.03.030
更新日期：2011-07-01 00:00:00
abstract：:Endodontic microsurgery has reduced the degree of treatment trauma compared with traditional apical surgery and further increased the success rate of natural teeth retention. However, when root apices of the mandibular posterior teeth are far from the buccal cortical bone surface or near to the inferior alveolar nerve...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.07.033
更新日期：2020-08-11 00:00:00
abstract：:This study investigated the sealing properties of cold laterally compacted gutta percha and Thermafil applied over different apically fractured rotary nickel-titanium files. Extracted human premolars were prepared by using ProTaper (Dentsply-Maillefer, Ballaigues, Switzerland) or ProFile (Dentsply-Maillefer) systems (...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.10.023
更新日期：2008-02-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effect of calcium hydroxide (CH) and antibiotic pastes, including a mixture of metronidazole and ciprofloxacin, with and without minocycline or cefaclor, on the dislocation resistance of mineral trioxide aggregate (MTA) to root dentin. METHODS:Eighty single-rooted...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.018
更新日期：2014-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the ability of MTA (Dentsply Maillefer, Tulsa, OK) and EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA) to set in the presence of human blood and minimal essential media. METHODS:A model was created using polymethyl methacrylate blocks each pr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.041
更新日期：2013-08-01 00:00:00
abstract：:A retrograde root-end cover with a special resin composite (Retroplast) combined with the dentin bonding agent Gluma (Bayer AG) has been used since 1984 by the authors. Its content of silver, added to promote radiopacity, has been found to lower the working time and storage stability of the composite and might cause d...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章
doi：10.1016/S0099-2399(96)80082-3
更新日期：1996-09-01 00:00:00
