当前位置： SCI文献检索 > JOURNAL OF ENDODONTICS期刊下所有文献 > KLF4 promotes the odontoblastic differentiation of human dental pulp cells.

KLF4 promotes the odontoblastic differentiation of human dental pulp cells.

Abstract：

INTRODUCTION:Krüppel-like factor 4 (KLF4) plays an important role in cytodifferentiation and proliferation. Our previous study showed that KLF4 was specifically expressed in polarizing and elongating odontoblasts. However, the role of KLF4 in odontoblast differentiation was still unknown. The purpose of this study was to investigate the role of KLF4 in odontoblastic differentiation of human dental pulp cells (hDPCs). METHODS:hDPCs were treated with odontoblastic induction medium. Odontoblastic differentiation was determined by the detection of alkaline phosphatase (ALPase) activity and the expression of mineralization-related genes including ALP, dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1). Also, cell proliferation ability was examined by the 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Simultaneously, messenger RNA and protein levels of KLF4 were detected. pKLF4-IRES2-EGFP plasmid encoding full-length KLF4 was constructed to overexpress KLF4, and biologic effects of KLF4 on hDPCs were investigated by the evaluation of ALPase activity and the detection of ALP, DSPP, and DMP-1 expression and analysis of cell proliferation ability. RESULTS:ALPase activity and the expression of odontoblastic differentiation markers progressively increased in hDPCs cultured with odontoblastic induction medium. Meanwhile, the proliferation ability decreased in this procedure; messenger RNA and protein levels of KLF4 increased significantly on day 5 after the odontoblastic induction of hDPCs and kept increasing until day 14. hDPCs showed up-regulated activity of ALPase and the expression of mineralization-related genes, including ALP, DMP-1, and dentin sialoprotein (DSP), after KLF4 overexpression. Besides, the proliferation ability of hDPCs decreased significantly in the KLF4 overexpression group by EdU incorporation assay. CONCLUSIONS:Our findings suggest that KLF4 is able to promote odontoblastic differentiation of hDPCs and inhibit proliferation of hDPCs.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Lin H,Xu L,Liu H,Sun Q,Chen Z,Yuan G,Chen Z

doi

10.1016/j.joen.2011.03.030

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

948-54

issue

7

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(11)00421-3

journal_volume

37

pub_type

杂志文章

相关文献

JOURNAL OF ENDODONTICS文献大全
  • Mandibular first molar with six separate canals.

    abstract：INTRODUCTION:Many anatomical variations can occur within the mandibular first molar. Commonly, 3-4 canals are located, but as many as 6-7 canals have been reported. METHODS:This report describes a case of a mandibular first molar with 6 separate canals (3 mesial and 3 distal) that was instrumented with conventional ha...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2011.03.005

    authors： Ryan JL,Bowles WR,Baisden MK,McClanahan SB

    更新日期：2011-06-01 00:00:00

  • Regenerative Endodontics: Burning Questions.

    abstract：:Pulp regeneration and its clinical translation into regenerative endodontic procedures are receiving increasing research attention, leading to significant growth of the published scientific and clinical literature within these areas. Development of research strategies, which consider patient-, clinician-, and scientis...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2017.06.002

    authors： Smith AJ,Cooper PR

    更新日期：2017-09-01 00:00:00

  • Accuracy of Cone-beam Computed Tomography in Measuring Dentin Thickness and Its Potential of Predicting the Remaining Dentin Thickness after Removing Fractured Instruments.

    abstract：INTRODUCTION:The purpose of this study was to evaluate the accuracy of cone-beam computed tomographic (CBCT) to measure dentin thickness and its potential of predicting the remaining dentin thickness after the removal of fractured instrument fragments. METHODS:Twenty-three human mandibular molars were selected, and 4-...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2017.03.041

    authors： Xu J,He J,Yang Q,Huang D,Zhou X,Peters OA,Gao Y

    更新日期：2017-09-01 00:00:00

  • Acquisition of anatomic parameters concerning molar pulp chamber landmarks using cone-beam computed tomography.

    abstract：INTRODUCTION:Cone-beam computed tomographic (CBCT) imaging is a valuable tool for endodontic therapy. The aim of this study was to verify whether clinical use of CBCT imaging can accurately acquire parameters concerning molar pulp chamber landmarks, which are important data to help start a successful access cavity and ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2014.04.002

    authors： Azim AA,Azim KA,Deutsch AS,Huang GT

    更新日期：2014-09-01 00:00:00

  • Mesenchymal chondrosarcoma mimicking apical periodontitis.

    abstract：:This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2008.08.016

    authors： Bueno MR,De Carvalhosa AA,De Souza Castro PH,Pereira KC,Borges FT,Estrela C

    更新日期：2008-11-01 00:00:00

  • Migration of a Streptococcus sanguis strain through the root dentinal tubules.

    abstract：:The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/s0099-2399(06)80460-7

    authors： Perez F,Calas P,de Falguerolles A,Maurette A

    更新日期：1993-06-01 00:00:00

  • Quantitative Assessment of Intracanal Regenerated Tissues after Regenerative Endodontic Procedures in Mature Teeth Using Magnetic Resonance Imaging: A Randomized Controlled Clinical Trial.

    abstract：INTRODUCTION:The present study quantitatively assessed tissue regeneration after regenerative endodontic procedures (REPs) with 2 different apical preparation sizes in mature teeth using magnetic resonance imaging (MRI). METHODS:Eighteen maxillary anterior mature necrotic teeth with periapical lesions were selected fo...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joen.2020.01.026

    authors： El-Kateb NM,El-Backly RN,Amin WM,Abdalla AM

    更新日期：2020-05-01 00:00:00

  • The effect of different irrigating solutions on bond strength of two root canal-filling systems.

    abstract：:The bond strength of ActiV GP root canal filling system and gutta-percha/AH plus sealer when used after final rinse with different irrigation protocols was evaluated in this study. Forty roots were randomly divided into four groups (n = 10) according to the final irrigation regimen: group 1, 5 mL 17% EDTA; group 2, 5 ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2009.01.003

    authors： Hashem AA,Ghoneim AG,Lutfy RA,Fouda MY

    更新日期：2009-04-01 00:00:00

  • Innate immune responses of the dental pulp to caries.

    abstract：:Various cells and inflammatory mediators are involved in the initial pulpal responses to caries. This review focuses on the cellular, neuronal, and vascular components of pulpal innate responses to caries. Discussion will include dentinal fluid, odontoblasts, neuropeptides, and neurogenic inflammation, which are not c...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,评审

    doi：10.1016/j.joen.2007.01.001

    authors： Hahn CL,Liewehr FR

    更新日期：2007-06-01 00:00:00

  • Proliferative ability and alkaline phosphatase activity with in vivo cellular aging in human pulp cells.

    abstract：:Little is known about the effect of aging on characteristic functions of pulp cells. When damaged pulp is recovered and mineralized tissue is formed to protect remaining pulp tissue, the general responses of pulp tissue after adequate stimuli (pulp cell proliferation and activation of alkaline phosphatase [ALPase]) ar...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200301000-00003

    authors： Shiba H,Nakanishi K,Rashid F,Mizuno N,Hino T,Ogawa T,Kurihara H

    更新日期：2003-01-01 00:00:00

  • Effectiveness of manual and rotary instrumentation techniques for cleaning flattened root canals.

    abstract：:The cleaning capacity of manual and rotary instrumentation techniques in mesial-distal flattened canals was studied by morphometric analysis. Twenty human mandibular incisors were divided into two groups of 10 teeth each: group 1, crown-down technique with rotary instrumentation using ProFile .04; group 2, crown-down ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200205000-00004

    authors： Barbizam JV,Fariniuk LF,Marchesan MA,Pecora JD,Sousa-Neto MD

    更新日期：2002-05-01 00:00:00

  • Effect of Platelet-rich Fibrin on Healing of Apicomarginal Defects: A Randomized Controlled Trial.

    abstract：INTRODUCTION:The purpose of this prospective, randomized controlled trial was to evaluate the healing outcomes of platelet-rich fibrin (PRF) in periapical surgeries involving apicomarginal defects and to compare these results with surgeries not using any guided tissue regeneration techniques. METHODS:Thirty patients w...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joen.2015.04.004

    authors： Dhiman M,Kumar S,Duhan J,Sangwan P,Tewari S

    更新日期：2015-07-01 00:00:00

  • Antibacterial Efficacy of a New Sonic Irrigation Device for Root Canal Disinfection.

    abstract：INTRODUCTION:Passive ultrasonic irrigation (PUI) is the most widespread method used to activate irrigation solutions. Concerns have been raised that PUI is less effective in curved root canals and is not passive at all. Our aim was to compare a novel passive sonic irrigation (PSI) device (6000 Hz) with PUI and manual i...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2016.08.024

    authors： Neuhaus KW,Liebi M,Stauffacher S,Eick S,Lussi A

    更新日期：2016-12-01 00:00:00

  • Odontogenic cutaneous sinus tract.

    abstract：:Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/s0099-2399(06)80959-3

    authors： Foster KH,Primack PD,Kulild JC

    更新日期：1992-06-01 00:00:00

  • Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis.

    abstract：:EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200112000-00007

    authors： Sceiza MF,Daniel RL,Santos EM,Jaeger MM

    更新日期：2001-12-01 00:00:00

  • Similar influence of stabilized alkaline and neutral sodium hypochlorite solutions on the fracture resistance of root canal-treated bovine teeth.

    abstract：INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2014.02.028

    authors： Souza EM,Calixto AM,Lima CN,Pappen FG,De-Deus G

    更新日期：2014-10-01 00:00:00

  • Machining efficiency of Flex-R, K-Flex, Trio-Cut, and S Files.

    abstract：:Many root canal instruments with several different designs have been introduced during recent years. This article evaluates the machining properties of four such instruments: the Flex-R, K-Flex, Trio-Cut, and S file. There are large variations in the machining properties of these instruments. The results are comparabl...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/s0099-2399(07)80200-7

    authors： Stenman E,Spångberg LS

    更新日期：1990-12-01 00:00:00

  • Endodontic retreatment of thermafil or lateral condensation obturations in post space prepared teeth.

    abstract：:The purpose of this study was to quantify the amount of remaining gutta-percha/sealer after retreatment of post space prepared teeth obturated with a lateral condensation technique or with Thermafil with plastic and metallic carrier. The time required for retreatment was also recorded. Forty-five extracted mandibular ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/s0099-2399(06)80019-1

    authors： Zuolo ML,Imura N,Ferreira MO

    更新日期：1994-01-01 00:00:00

  • Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations.

    abstract：:Amalgam, IRM, and a mineral trioxide aggregate were tested for repair of experimentally created root perforations. Fifty sound, extracted mandibular and maxillary molars were used in this study. A perforation was created on the mesial root surface at about a 45-degree angle to the long axis of each tooth. The tooth wa...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)81282-3

    authors： Lee SJ,Monsef M,Torabinejad M

    更新日期：1993-11-01 00:00:00

  • Comparison of five cone beam computed tomography systems for the detection of vertical root fractures.

    abstract：INTRODUCTION:This study compared the accuracy of cone beam computed tomography (CBCT) scans made by five different systems in detecting vertical root fractures (VRFs). It also assessed the influence of the presence of root canal filling (RCF), CBCT slice orientation selection, and the type of tooth (premolar/molar) on ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2009.09.013

    authors： Hassan B,Metska ME,Ozok AR,van der Stelt P,Wesselink PR

    更新日期：2010-01-01 00:00:00

  • Lipopolysaccharide enhances decorin expression through the Toll-like receptor 4, myeloid differentiating factor 88, nuclear factor-kappa B, and mitogen-activated protein kinase pathways in odontoblast cells.

    abstract：INTRODUCTION:Lipopolysaccharide (LPS) has been shown to regulate the function of odontoblasts. However, the molecular mechanisms of the effect of LPS on odontoblasts are poorly understood. Decorin (DCN), one of the major matrix proteoglycans, is known to affect the mineralization of teeth. In this study, we investigate...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2011.12.021

    authors： He W,Qu T,Yu Q,Wang Z,Wang H,Zhang J,Smith AJ

    更新日期：2012-04-01 00:00:00

  • Role of pH Changes on Transforming Growth Factor-β1 Release and on the Fibrin Architecture of Platelet-rich Fibrin When Layered with Biodentine, Glass Ionomer Cement, and Intermediate Restorative Material.

    abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of pH that is due to setting reaction of Biodentine, glass ionomer cement (GIC), and intermediate restorative material (IRM) on transforming growth factor-β1 (TGF-β1) release and on the fibrin architecture of platelet-rich fibrin (PRF). METHODS:PRF w...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2016.02.009

    authors： Mullaguri H,Suresh N,Surendran S,Velmurugan N,Chitra S

    更新日期：2016-05-01 00:00:00

  • Role of Protein Phosphatase 1 in Angiogenesis and Odontoblastic Differentiation of Human Dental Pulp Cells.

    abstract：INTRODUCTION:The aims of this study were to examine the immunolocalization of protein phosphatase 1 (PP1) in developing mouse pulp tissue and to explore the role of PP1 in odontoblastic differentiation and in vitro angiogenesis in human dental pulp cells (HDPCs). METHODS:Immunolocalization of PP1 was assessed in devel...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2016.10.013

    authors： Kim JY,Kim DS,Auh QS,Yi JK,Moon SU,Kim EC

    更新日期：2017-03-01 00:00:00

  • A Comparison of Apical Bacterial Extrusion in Manual, ProTaper Rotary, and One Shape Rotary Instrumentation Techniques.

    abstract：INTRODUCTION:Apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. Many factors affect the amount of extruded intracanal materials. The purpose of this study was t...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2015.09.002

    authors： Mittal R,Singla MG,Garg A,Dhawan A

    更新日期：2015-12-01 00:00:00

  • Comparison of apical leakage in teeth obturated with a polyamide varnish or zinc oxide and eugenol cement using lateral condensation.

    abstract：:The sealing ability of copal varnish has been investigated when used as an adjunct in restorative procedures, retrofilling procedures, and dontic obturation procedures. Barrier, a polyamide-type polymer cavity varnish, has been compared with copal varnishes in restorative dentistry. This investigation compared the api...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/S0099-2399(06)81138-6

    authors： Smith DW,Wong M

    更新日期：1992-01-01 00:00:00

  • Characterization of an ex vivo model for the assessment of root canal disinfection.

    abstract：:Root canal bacteria in teeth with apical periodontitis were enumerated after extraction and incubation. Canals in 36 teeth were sampled after: S1, incubation for 2 hours (group A), 2 days (group B), 4 days (group C), and 6 days (group D); S2, subsequent incubation for 1 week; S3, canal disinfection; and S4, final incu...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2008.08.038

    authors： Villette G,Manek S,Legner M,Fillery ED,Torneck CD,Basrani BR,Friedman S

    更新日期：2008-12-01 00:00:00

  • Organic Tissue Dissolution in Clodronate and Etidronate Mixtures with Sodium Hypochlorite.

    abstract：INTRODUCTION:Mixtures of clodronate with sodium hypochlorite (NaOCl) better maintain free available chlorine (FAC) than etidronate-hypochlorite mixtures. This research aimed to compare organic tissue dissolution and residual FAC between clodronate and etidronate mixtures. Additionally, clodronate-hypochlorite mixtures ...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2019.10.020

    authors： Wright PP,Scott S,Kahler B,Walsh LJ

    更新日期：2020-02-01 00:00:00

  • Apically Extruded Debris after Retreatment Procedure with Reciproc, ProTaper Next, and Twisted File Adaptive Instruments.

    abstract：INTRODUCTION:The aim of this study was to compare the amount of debris extruded from the apex during retreatment procedures with ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland), Reciproc (RCP; VDW, Munich, Germany), and Twisted File Adaptive (TFA; SybronEndo, Orange, CA) files and the duration of these...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1016/j.joen.2016.12.003

    authors： Yılmaz K,Özyürek T

    更新日期：2017-04-01 00:00:00

  • Improving the seal of amalgam cores with cemented dowels: a comparative in vitro radioactive tracer study.

    abstract：:Amalgam cores with cemented dowels are commonly used to restore endodontically treated teeth. These restorations were widely studied for their mechanical properties; however, less is known about their sealing ability. The effect of the cement type used for the dowel and that of a bonding agent application were studied...

    journal_title：Journal of endodontics

    pub_type： 杂志文章

    doi：10.1097/00004770-200104000-00015

    authors： Metzger Z,Schaham G,Abramovitz I,Dotan M,Ben-Amar A

    更新日期：2001-04-01 00:00:00

  • Outcome of Endodontic Surgery: A Meta-analysis of the Literature-Part 3: Comparison of Endodontic Microsurgical Techniques with 2 Different Root-end Filling Materials.

    abstract：INTRODUCTION:The aim of the present study was to investigate the influence of root-end preparation and filling material on endodontic surgery outcome. A systematic review and meta-analysis was conducted to determine the outcome of resin-based endodontic surgery (RES, the use of high-magnification preparation of a shall...

    journal_title：Journal of endodontics

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.joen.2018.02.021

    authors： Kohli MR,Berenji H,Setzer FC,Lee SM,Karabucak B

    更新日期：2018-06-01 00:00:00