Abstract:
INTRODUCTION:The purpose of this study was to compare the cell viability of dental pulp cells treated with Biodentine (Septodont, Saint-Maur, France) and mineral trioxide aggregate (MTA) and the in vitro and in vivo expression of mineralization markers induced by the 2 materials. METHODS:Human dental pulp cells isolated from 6 permanent teeth were stimulated with Biodentine and MTA extracts. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, and quantitative reverse-transcriptase polymerase chain reaction was used to determine the expression of mineralization markers. Specimens of teeth from dogs treated with Biodentine and MTA after pulpotomy were used to determine the presence of osteopontin and alkaline phosphatase by immunohistochemistry and runt-related transcription factor 2 by immunofluorescence. RESULTS:No significant differences in cell viability were found between MTA and Biodentine extracts and controls after 24 and 48 hours (P > .05). After 48 hours, osteopontin (SPP1), alkaline phosphatase (ALP), and runt-related transcription factor 2 (RUNX2) expression was higher in MTA and Biodentine than in controls (P < .05). Osteopontin staining was more intense and spread over a greater number of areas in Biodentine than in MTA samples (P < .0001). Alkaline phosphatase staining of a mineralized tissue bridge was significantly different between materials (P < .0001), but no difference in alkaline phosphatase staining of pulp tissue was found between MTA and Biodentine (P = .2). Also, no significant difference in the number of cells labeled for runt-related transcription factor 2 by immunofluorescence was observed between materials (P > .05). CONCLUSIONS:Biodentine stimulated similar markers as MTA, but staining was more intense and spread over a larger area of the pulp tissue.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Daltoé MO,Paula-Silva FW,Faccioli LH,Gatón-Hernández PM,De Rossi A,Bezerra Silva LAdoi
10.1016/j.joen.2015.12.018subject
Has Abstractpub_date
2016-04-01 00:00:00pages
596-603issue
4eissn
0099-2399issn
1878-3554pii
S0099-2399(15)01148-6journal_volume
42pub_type
杂志文章abstract::The aim of this in vivo study was to find a way to conduct qualitative and quantitative evaluations of radicular apical third apposition in rats. Twenty rats were injected intraperitoneally with tetracycline at weight-adjusted doses. Mesio-distal sections of the first mandibular molar regions were prepared. The 60 to ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80153-8
更新日期:1999-04-01 00:00:00
abstract:INTRODUCTION:Autotransplantation is a viable option for treating missing teeth when a donor tooth is available. This retrospective study reports the success rate for the autotransplantation of 19 molars with complete root formation. METHODS:The study enrolled 19 patients (11 men and 8 women) in whom 19 molars were tra...
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journal_title:Journal of endodontics
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doi:10.1016/S0099-2399(99)80306-9
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journal_title:Journal of endodontics
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更新日期:2002-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the efficacy of the cutting ability of two different instruments, concerning changes in the dentin thickness removed and root canal volume, by using multislice computed tomography scanning. METHODS:Thirty single-rooted mandibular premolars were divided into two equ...
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journal_title:Journal of endodontics
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doi:10.1016/j.joen.2014.01.003
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:The introduction of nickel-titanium (NiTi) files into clinical practice has improved the quality of canal shaping, but increasing the curvature of the root canal (or the diameter of the master instrument that prepares the full working length) could result in more transportation, straightening, and aberrati...
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doi:10.1016/j.joen.2011.06.030
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to evaluate the time required by four different root canal medications coupled with the temporary filling material Cavit (ESPE, Seefeld, Germany) to prevent penetration of bacteria into the root canal. There were 145 roots prepared in a standardized manner. Four groups with 15 samples each we...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.023
更新日期:2006-02-01 00:00:00
abstract::The aim of this in vitro study was to investigate the effect of the smear layer on apical microleakage in teeth obturated with mineral trioxide aggregate (MTA). Fifty single-rooted central maxillary teeth were used in this study. All teeth were instrumented to size 60 by using the step-back technique. The selected tee...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2008.08.022
更新日期:2008-12-01 00:00:00
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更新日期:2020-04-01 00:00:00
abstract::Taurodontism is a morphoanatomical change in the shape of a tooth, which usually occurs in multirooted teeth. An enlarged body and pulp chamber, as well as apical displacement of the pulpal floor, are characteristic features. Endodontic treatment of a taurodont tooth is challenging, because it requires special care in...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200305000-00009
更新日期:2003-05-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the fracture resistance of cast posts versus the fracture resistance of fiber posts by means of meta-analysis when they were used in the restoration of endodontically treated teeth. METHODS:MEDLINE, Cochrane Controlled Trials Register, China National Knowledge Infrastr...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.joen.2012.09.026
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:This study aimed to evaluate the precision of electronic apex locators (EALs) in locating the apical constriction (AC) during a root canal treatment compared with a histologic evaluation of the AC as well as the effects of possible influencing factors by means of a systematic review of the literature and m...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.joen.2015.08.012
更新日期:2015-11-01 00:00:00
abstract::A nested polymerase chain reaction (PCR)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of Pseudoramibacter alactolyticus. Identification by nested PCR was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acut...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200311000-00012
更新日期:2003-11-01 00:00:00
abstract:INTRODUCTION:The aim of this prospective study was to evaluate the postoperative pain that followed root canal treatments performed with a single-file reciprocating system on asymptomatic uniradicular necrotic teeth with and without foraminal enlargements (FEs). METHODS:Forty-six volunteers were randomly divided into ...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.09.018
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 μg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capac...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.027
更新日期:2014-03-01 00:00:00
abstract:INTRODUCTION:Root fusion is an anatomic variation in maxillary second molars (MSMs); however, the nature of this canal morphology as it relates to its root anatomy has not been fully clarified. The purpose of this study was to investigate the relationship between features of fused roots and root canal anatomy in MSMs u...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.035
更新日期:2014-06-01 00:00:00
abstract:INTRODUCTION:A limited number of in vivo studies have discussed the prevalence of middle mesial canals in root canal systems of mandibular molars. The reported results have varied between 1% and 25%, with no detailed description of the depth and direction of troughing needed to identify such small canal orifices. The o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.09.013
更新日期:2015-02-01 00:00:00
abstract:INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.026
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:This clinical study was conducted to measure the endotoxin levels in infected root canals (RCs) and exudates related to acute apical abscesses (AAAs). In addition, the effectiveness of RC procedures in reducing the endotoxin levels in RCs was monitored. METHODS:Paired samples of infected RCs and exudates ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.008
更新日期:2014-02-01 00:00:00
abstract::An examination of 5616 endodontically treated and retreated maxillary first and second molars was made in an attempt to determine the percentage of MB2 canals that could be located routinely, and evaluate if there were any significant differences between initial treatments and retreatments. The teeth examined were 357...
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pub_type: 杂志文章
doi:10.1097/01.don.0000140581.38492.8b
更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joen.2015.08.028
更新日期:2015-12-01 00:00:00
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journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.011
更新日期:2018-12-01 00:00:00
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journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.11.022
更新日期:2011-03-01 00:00:00
abstract::This case series reports the outcomes of 8 patients (ages 9-14 years) who presented with 9 immature permanent teeth with pulpal necrosis and apical periodontitis. During treatment, 5 of the teeth were found to have at least some residual vital tissue remaining in the root canal systems. After NaOCl irrigation and medi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.03.023
更新日期:2008-07-01 00:00:00
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journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.07.023
更新日期:2018-11-01 00:00:00
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journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.03.016
更新日期:2016-06-01 00:00:00
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journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80069-0
更新日期:1996-12-01 00:00:00
abstract::The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200012000-00011
更新日期:2000-12-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the accuracy of cone-beam computed tomographic (CBCT) to measure dentin thickness and its potential of predicting the remaining dentin thickness after the removal of fractured instrument fragments. METHODS:Twenty-three human mandibular molars were selected, and 4-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.03.041
更新日期:2017-09-01 00:00:00