Abstract:
:There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers for odontoblast differentiation. The isolated DPCs expressed mesenchymal stem-cell markers as shown by flow cytometry and could differentiate in vitro into odontogenic, adipogenic, and chondrogenic lineages. Alkaline phosphatase activity of DPCs elevated over time, with significant upregulation on day 21 in odontogenic induction. Quantitative RT-PCR revealed that osteocalcin, dentin sialophosphoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) expression also increased time dependently in the induction cultures. In conclusion, we isolated DPCs with stem cell characteristics. MEPE and DSPP showed a similar regulatory pattern of DPCs mineralization. MEPE along with DSPP may be potential odontogenetic differentiation markers.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wei X,Ling J,Wu L,Liu L,Xiao Ydoi
10.1016/j.joen.2007.02.009subject
Has Abstractpub_date
2007-06-01 00:00:00pages
703-8issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(07)00167-7journal_volume
33pub_type
杂志文章abstract:INTRODUCTION:Recent evidence finds mineral trioxide aggregate (MTA) more effective than calcium hydroxide (CH) for direct pulp capping (DPC). The present study assessed the cost-effectiveness of MTA versus CH for DPC using a model-based simulation approach. METHODS:A mixed public/private payer perspective in the conte...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.08.019
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:This study quantitatively evaluated the type and amount of image artifacts generated by different intracanal materials in birooted teeth scanned at different exposure parameters. METHODS:The sample consisted of 15 birooted premolars. Seven different intracanal material combinations were used in each tooth...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.11.007
更新日期:2019-02-01 00:00:00
abstract::The present survey was conducted to obtain answers to some basic questions regarding the use of intracanal medications, root canal filling methods, and the intracanal breakage of instruments. A letter was sent to 300 endodontists listed in the 1995-1996 membership roster of the American Association of Endodontists. Ei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80306-9
更新日期:1999-12-01 00:00:00
abstract:INTRODUCTION:This finite element analysis study was aimed at evaluating the effects of rotary systems on stresses in photopolymerized resin root models with oval-shaped canals. METHODS:Among the data collected by computed tomographic imaging, a mandibular second premolar tooth with an oval-shaped canal was selected, r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.007
更新日期:2016-08-01 00:00:00
abstract::The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 x 1 mm). For cytotoxicity testing (MTT method), the spe...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.05.006
更新日期:2006-10-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess, by scanning electron microscopic (SEM) analysis, the ability of 17% EDTA and 7% maleic acid in the removal of the smear layer from the human root canal system. METHODS:Eighty single-rooted human anterior teeth were subjected to standardized root canal instrumentation (...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2009.07.021
更新日期:2009-11-01 00:00:00
abstract:INTRODUCTION:An ideal orthograde or retrograde filling material should seal the pathways of communication between the root canal system and its surrounding tissues. It should also be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable. Mineral trioxide aggregate ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.09.006
更新日期:2010-01-01 00:00:00
abstract:INTRODUCTION:The purpose of this retrospective study was to determine the success of the inferior alveolar nerve (IAN) block using either 3.6 mL or 1.8 mL 2% lidocaine with 1:100,000 epinephrine in patients presenting with symptomatic irreversible pulpitis. METHODS:As part of 7 previously published studies, 319 emerge...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.04.007
更新日期:2013-08-01 00:00:00
abstract::The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80565-0
更新日期:1995-04-01 00:00:00
abstract::The present study evaluates the morphological changes in root canal walls and temperature changes at root surfaces as a result of intracanal irradiation by erbium,chromium:YSGG laser under various conditions in vitro. Sixty single-rooted human teeth were examined. Root canals were prepared, and laser irradiation was p...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200101000-00003
更新日期:2001-01-01 00:00:00
abstract::Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.037
更新日期:2006-03-01 00:00:00
abstract::Locating the number and position of orifices on pulp-chamber floors can be difficult. This is especially true when the tooth being treated is heavily restored, malposed, or calcified. After evaluating 500 pulp chambers of extracted teeth, new laws for finding pulp chambers and root-canal orifices are proposed. The use...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200401000-00002
更新日期:2004-01-01 00:00:00
abstract::A case is presented in which endodontic treatment of a maxillary lateral incisor with a talon cusp and two root canals failed following a mistaken diagnosis. What was first diagnosed as an endodontic lesion was, in all probability, a primary periodontal lesion caused by the advance of bacteria from the gingival crevic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81947-3
更新日期:1990-07-01 00:00:00
abstract::This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80158-X
更新日期:1989-12-01 00:00:00
abstract::The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.11.005
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:The outcome of periapical surgery has been directly improved with the introduction of novel material formulations. The aim of the study was to compare the retrograde obturation quality of the following materials: calcium silicate (Biodentine; Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.022
更新日期:2018-05-01 00:00:00
abstract::Understanding the chemical and physical characteristics of set mineral trioxide aggregate (MTA) surface can provide insight into its bioactivity. The purpose of this study was to describe the surface chemistry and morphology of gray and white MTA set in the presence of fetal bovine serum (FBS). Eight MTA blocks were p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.09.013
更新日期:2008-01-01 00:00:00
abstract::This randomized, double-blind trial tested the null hypothesis that speed of deposition has no influence on the injection discomfort, efficacy, distribution, and duration of pulp anesthesia after incisive/mental nerve block in adult volunteers. Thirty-eight subjects received incisive/mental nerve blocks of 2.0 mL lido...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.07.016
更新日期:2007-10-01 00:00:00
abstract:INTRODUCTION:Patient preferences should be taken into account by clinicians when treatment planning. The purposes of this study were to describe the number of visits patients preferred when undergoing root canal therapy (RCT) and to assess whether their preferences were related to hypothetical treatment success rates. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.06.038
更新日期:2012-10-01 00:00:00
abstract::The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited ca...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000155259.22746.ae
更新日期:2005-09-01 00:00:00
abstract:INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.011
更新日期:2014-02-01 00:00:00
abstract:INTRODUCTION:The presence of cracks and fractures in teeth can pose difficulties in diagnosis, prognosis assessment, and treatment recommendations. When a tooth has no significant restorations or caries, whereby the pulp is nonvital in the absence of a luxation injury, it is suggested that this necrosis is likely cause...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.12.018
更新日期:2010-03-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the root canal preparation in flat-oval canals treated with either rotary or self-adjusting file (SAF) by using micro-tomography analysis. METHODS:Forty mandibular incisors were scanned before and after root canal instrumentation with rotary instruments (n = 20) or SA...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.03.017
更新日期:2011-07-01 00:00:00
abstract::This case report describes the clinical, radiologic, and histopathologic features of the glandular odontogenic cyst (GOC). Although a relatively rare lesion not previously reported in the endodontic literature, the GOC is recommended for inclusion in a differential diagnosis of a dentoalveolar radiolucency. The GOC is...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200403000-00014
更新日期:2004-03-01 00:00:00
abstract::Periapical lesions usually are caused by root canal infection; nevertheless, other pathologies may eventually involve the tooth apex, making the correct diagnosis more difficult. Glandular odontogenic cysts (GOCs) are uncommon and, despite their cystic nature, may present an aggressive behavior and a high recurrence r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.06.015
更新日期:2017-11-01 00:00:00
abstract:INTRODUCTION:Experimental studies show that dental pulp cells respond unfavorably to contact with resin composite restorative material. Hypothetically, in a random population, the frequency of apical periodontitis should be higher for teeth restored with resin composite than with amalgam. Therefore, the aim was to comp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.05.002
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2017.11.002
更新日期:2018-03-01 00:00:00
abstract::This study is a quantitative assessment of the luminous energy transmitted through different translucent fiber posts. After embedding the posts in black resin, the blocks were submitted to sequential cuts in a precision machine, and depths of 16 mm, 12 mm, 8 mm, and 4 mm were assessed for light transmission with a dig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.12.007
更新日期:2008-03-01 00:00:00
abstract::The purpose of this study was to evaluate the effect of citric acid and EDTA solutions on the microhardness and the roughness of human root canal dentin. Forty-five human teeth sectioned longitudinally were used. Specimens were randomly divided into three groups of 30 teeth each and were treated as follows: (a) one mo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000136212.53475.ad
更新日期:2005-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the ability of MTA (Dentsply Maillefer, Tulsa, OK) and EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA) to set in the presence of human blood and minimal essential media. METHODS:A model was created using polymethyl methacrylate blocks each pr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.04.041
更新日期:2013-08-01 00:00:00