Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications.

Abstract:

:Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulose nitrate membrane filters. Each biofilm-containing membrane was thoroughly covered with 1 ml of the test medications and incubated for 1 day at 37 degrees C. Treated biofilms were then aseptically transferred to vials containing a neutralizing agent in saline solution and vortexed. Suspensions were 10-fold diluted, seeded onto Mitis salivarius agar plates, and the colony-forming units counted after 48 h of incubation. There were significant differences between the formulations tested. The association of clindamycin with metronidazole significantly reduced the number of cells in 1-day biofilms. However of all medications tested, only 2% chlorhexidine-containing medications were able to thoroughly eliminate most of both 1-day and 3-day E. faecalis biofilms.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Lima KC,Fava LR,Siqueira JF Jr

doi

10.1097/00004770-200110000-00004

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

616-9

issue

10

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)61016-3

journal_volume

27

pub_type

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