Abstract:
INTRODUCTION:We evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old Enterococcus faecalis biofilm. METHODS:E. faecalis biofilm was grown on standardized dentin samples for 3 weeks. Infected dentin samples were randomized into 8 experimental groups (n = 8) and treated with calcium hydroxide (Ca[OH]2), 500 mg/mL of double antibiotic paste (DAP, equal portions of metronidazole and ciprofloxacin), low dilutions of DAP (1 or 0.1 mg/mL loaded into a methylcellulose vehicle system), sterile saline, or placebo paste (only methylcellulose) for 7 days. The other experimental groups were treated with 1.5% sodium hypochlorite (NaOCl) or 2% chlorhexidine gluconate (CHX) solutions for 5 minutes. After the assigned treatments, the bacterial biofilms were detached from dentin, spiral plated, and quantified using an automated counting machine. Permutation tests followed by Sidak post hoc multiple comparisons were used for statistical analyses (α = 0.05). RESULTS:The infected dentin treated with 1.5% NaOCl or 500 mg/mL of DAP provided complete eradication of bacterial biofilm. Furthermore, the infected dentin treated with 2% CHX, Ca(OH)2, or 1 mg/mL of DAP had a comparable antibiofilm effect, but they were not able to completely eradicate bacterial biofilm. No significant difference in the antibiofilm effect was observed between 500 mg/mL of DAP, Ca(OH)2, 1.5% NaOCl, and 2% CHX. CONCLUSIONS:At least 1 mg/mL of DAP in a methylcellulose vehicle system is required to eliminate a substantial amount of E. faecalis biofilm. Furthermore, the antibiofilm effects of 1.5% NaOCl and 2% CHX irrigation solutions were comparable with that of 500 mg/mL of DAP and Ca(OH)2.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Tagelsir A,Yassen GH,Gomez GF,Gregory RLdoi
10.1016/j.joen.2015.09.023subject
Has Abstractpub_date
2016-02-01 00:00:00pages
258-62issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(15)00898-5journal_volume
42pub_type
杂志文章abstract::Root canal bacteria in teeth with apical periodontitis were enumerated after extraction and incubation. Canals in 36 teeth were sampled after: S1, incubation for 2 hours (group A), 2 days (group B), 4 days (group C), and 6 days (group D); S2, subsequent incubation for 1 week; S3, canal disinfection; and S4, final incu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.038
更新日期:2008-12-01 00:00:00
abstract:INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.028
更新日期:2014-10-01 00:00:00
abstract::Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80268-4
更新日期:1999-06-01 00:00:00
abstract:INTRODUCTION:Buffered local anesthetics have not been studied for incision and drainage procedures in dentistry. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and pain of an incision and drainage procedure by using a buffered versus a nonbuffered 2% lidocaine wi...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2013.07.008
更新日期:2013-11-01 00:00:00
abstract::Extracanal invasive resorption is not a well-defined phenomenon. This article describes its clinical, radiographic, and histological characteristics. Treatment of affected teeth based on the location of the resorptive defect is also described. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80056-3
更新日期:1998-07-01 00:00:00
abstract:INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.09.020
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:Apical periodontitis is an inflammation and destruction of periapical tissues. Matrix metalloproteinase-9 (MMP-9) is thought to be involved in periapical lesion formation and progression. The aim of this study was to evaluate the lesion progression in MMP-9 knockout (KO) mice compared with that in control ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.003
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:External apical root resorption (EARR) is a frequent iatrogenic effect of orthodontic treatment. The way root-filled teeth respond to orthodontic forces with respect to EARR has been reported as varying widely between individuals. Genetic variants in the interleukin-1 gene have been associated with an incr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.12.022
更新日期:2012-03-01 00:00:00
abstract:INTRODUCTION:This randomized clinical trial assessed the effect of preoperative intraoral cryotherapy application on the success rate of inferior alveolar nerve blocks (IANBs) in patients with symptomatic irreversible pulpitis (SIP). METHODS:One hundred four patients with SIP were randomly distributed into 2 groups: c...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2019.05.001
更新日期:2019-08-01 00:00:00
abstract:INTRODUCTION:Periodontal mechanoreceptors (PMRs) are refined neural receptors present in abundance at the root apex and have a pivotal role in oral fine motor control. This case-control study aimed to evaluate the oral fine motor control of teeth treated with endodontic microsurgery (EMS) in comparison with the control...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2020.10.022
更新日期:2021-02-01 00:00:00
abstract::The objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. DNA was extracted from 45 canal samples of root-filled teet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.01.016
更新日期:2008-05-01 00:00:00
abstract::The purpose of this study was to evaluate the ultramorphological and chemical changes in Er,Cr:YSGG laser-treated radicular dentin in comparison with two different irrigation regimes. Extracted human premolars were assigned into three experimental groups. Group 1: After each file size, root canals were irrigated with ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.08.005
更新日期:2006-05-01 00:00:00
abstract::Fusion and gemination are developmental anomalies of the dental hard tissues that might require endodontic and surgical treatment for functional, orthodontic, or esthetic reasons. In this article, we report a rare case of successful endodontic management of unilateral fused mandibular second molar with a paramolar. Th...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.06.021
更新日期:2007-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this systematic review was to compare the clinical and radiographic outcomes of nonsurgical retreatment with those of endodontic surgery to determine which modality offers more favorable outcomes. METHODS:The study began with targeted electronic searches of MEDLINE, PubMed, and Cochrane dat...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.joen.2009.04.023
更新日期:2009-07-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess the root canal configurations in permanent mandibular lateral incisors (PMLIs) and the correlation between the root canal configurations of PMLIs with the appearance of a distolingual root (DLR) in permanent mandibular first molars (PMFMs) using cone-beam computed tomogr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.08.027
更新日期:2018-01-01 00:00:00
abstract::Non-surgical endodontic treatment represents a cornerstone of contemporary endodontic care. Reflecting the central importance of this therapy, there is a high number of studies that address issues related to this treatment. Unfortunately, many of these studies are preclinical "bench top" studies with largely undefined...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.05.019
更新日期:2009-08-01 00:00:00
abstract:INTRODUCTION:Pulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via G...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.003
更新日期:2020-01-01 00:00:00
abstract::This article describes how to construct and use a simplified model to demonstrate the operation of electronic root canal measuring devices. The model is effective, more economical than those previously described, and uses readily available materials. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81292-6
更新日期:1993-11-01 00:00:00
abstract::The difference in resistance to bacterial invasion into the dentinal tubules between vital and nonvital teeth has not been determined. This study was conducted to clarify the effect of vital pulp on bacterial invasion into the dentinal tubules. The specimens were 19 intact pairs of bilateral upper third molars of 19 h...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81098-8
更新日期:1995-02-01 00:00:00
abstract:INTRODUCTION:The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-α], interleukin [IL]-1β, and IL-6) in human apical periodont...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.10.015
更新日期:2019-02-01 00:00:00
abstract:INTRODUCTION:This clinical study was conducted to measure the endotoxin levels in infected root canals (RCs) and exudates related to acute apical abscesses (AAAs). In addition, the effectiveness of RC procedures in reducing the endotoxin levels in RCs was monitored. METHODS:Paired samples of infected RCs and exudates ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.008
更新日期:2014-02-01 00:00:00
abstract:INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2020.05.008
更新日期:2020-08-01 00:00:00
abstract::Thirteen teeth in human cadaver sections were accessed and #15 K-files were placed at various lengths in one root canal of each tooth. Using a positioner to ensure duplicate views, radiographs were taken with both Ultraspeed and Ektaspeed Plus X-ray film. Each of the 26 radiographs was coded and then placed in random ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80216-1
更新日期:1998-01-01 00:00:00
abstract::The purpose of this study was to evaluate the influence of Hero 642 rotary Ni-Ti instruments driven at 300, 400, or 600 rpm on root canal straightening, loss of working length, and instrument breakage. Sixty mesial root canals from extracted human mandibular molars were divided into 3 groups of 20 each and were instru...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200307000-00005
更新日期:2003-07-01 00:00:00
abstract::Simulated furcation perforations were repaired with and without blood contamination utilizing tooth-colored mineral trioxide aggregrate (MTA) mixed with either MTA liquid (sterile water), lidocaine, or saline. Samples underwent Instron testing at either 24 or 72 hours and at 7 days. Data were analyzed using a 3-way AN...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.09.007
更新日期:2006-05-01 00:00:00
abstract:INTRODUCTION:Sphingosine-1-phosphate receptor 1 (S1P1) is crucial for regulation of immunity and bone metabolism. This study aimed to investigate the expression of S1P1 in rat periapical lesions and its relationship with receptor activator of nuclear factor kappa B ligand (RANKL) and regulatory T (Treg) cells. METHODS...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.10.010
更新日期:2015-04-01 00:00:00
abstract::The purpose of the study was to evaluate the biocompatibility of two current adhesive resins and a calcium hydroxide cement. Fifty-four polyethylene tubes were filled with these dental materials, which were hand-mixed or light-cured according to the manufacturer's directions: group 1--Clearfill Liner Bond 2 (Kuraray);...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200009000-00006
更新日期:2000-09-01 00:00:00
abstract:INTRODUCTION:Securing a reproducible glide path before instrumentation is recommended to maintain the original geometry of the root canal system and to prevent file separation. Mechanical glide path management systems have been introduced to expedite this step. The aim of this study was to compare apical transportation...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.07.014
更新日期:2015-11-01 00:00:00
abstract:INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, an...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.08.010
更新日期:2017-12-01 00:00:00
abstract:INTRODUCTION:The aim of this retrospective study was to evaluate the outcome of endodontic microsurgery and to examine prognostic factors related to healing. METHODS:The clinical records of all patients who had undergone endodontic microsurgery from 1997-2003 at the National Dental Centre of Singapore were examined. T...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.04.005
更新日期:2014-08-01 00:00:00