Abstract：
INTRODUCTION:It is important to develop an antimicrobial agent without any damage on dental pulp. In the present study, we examined whether pretreatment of bacterial lipopolysaccharides (LPS) with ozonated water (O(3)aq) improves LPS-induced responses of rat odontoblastic cell line, KN-3. METHODS:After the pretreatment of LPS with O(3)aq, effects of LPS and O(3)aq-treated LPS on cell viability; calcification ability; expression of cyclooxygenase 2 (COX-2), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-alpha); and activation of p38 of KN-3 cells were examined. RESULTS:The formation of mineralized nodules by KN-3 cells was suppressed by LPS, whereas that suppression was inhibited by the pretreatment of LPS with ozonated water. We also found that LPS-induced expression of COX-2, IL-6, and TNF-alpha and p38 activation were markedly suppressed when LPS was pretreated with ozonated water. Furthermore, expression of COX-2, IL-6, and TNF-alpha by LPS were mainly induced through p38 activation. CONCLUSION:These results suggest that odontoblastic cells exhibit inflammatory responses against LPS and that ozonated water has the ability to improve LPS-induced inflammatory responses and suppression of odontoblastic properties of KN-3 cells through direct inhibition of LPS.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Noguchi F,Kitamura C,Nagayoshi M,Chen KK,Terashita M,Nishihara Tdoi
10.1016/j.joen.2009.01.016subject
Has Abstractpub_date
2009-05-01 00:00:00pages
668-72issue
5eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00089-2journal_volume
35pub_type
杂志文章abstract：:Taurodontism is a morphoanatomical change in the shape of a tooth, which usually occurs in multirooted teeth. An enlarged body and pulp chamber, as well as apical displacement of the pulpal floor, are characteristic features. Endodontic treatment of a taurodont tooth is challenging, because it requires special care in...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200305000-00009
更新日期：2003-05-01 00:00:00
abstract：INTRODUCTION:This study aimed to examine the dynamic recovery of established multispecies biofilms of oral bacteria after an initial treatment by D-enantiomeric peptide DJK-5, L-enantiomeric peptide 1018, or chlorhexidine digluconate (CHX). METHODS:Oral biofilms from 2 donors were grown on collagen-coated hydroxyapati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.11.020
更新日期：2020-11-25 00:00:00
abstract：INTRODUCTION:Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin. M...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.011
更新日期：2012-09-01 00:00:00
abstract：:The 4- to 6-yr outcome of initial (first-time) endodontic treatment was assessed for Phase II of the "Toronto Study." In total, 442 teeth were treated by using flared preparation and vertical compaction of warm gutta-percha or step-back preparation and lateral compaction. With 126 teeth excluded (discontinuers: deceas...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200405000-00002
更新日期：2004-05-01 00:00:00
abstract：:Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000128749.50151.24
更新日期：2004-12-01 00:00:00
abstract：INTRODUCTION:Apical extrusion of irrigants and debris is an inherent limitation associated with cleaning and shaping of root canals and has been studied extensively because of its clinical relevance as a cause of flare-ups. Many factors affect the amount of extruded intracanal materials. The purpose of this study was t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.09.002
更新日期：2015-12-01 00:00:00
abstract：:The purpose of the study was to evaluate the biocompatibility of two current adhesive resins and a calcium hydroxide cement. Fifty-four polyethylene tubes were filled with these dental materials, which were hand-mixed or light-cured according to the manufacturer's directions: group 1--Clearfill Liner Bond 2 (Kuraray);...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200009000-00006
更新日期：2000-09-01 00:00:00
abstract：:The aim of this study was to investigate the prevalence and configuration of the C-shaped canal using serial axial computed tomography images of the mandibular second molars that had not been restored severely or treated endodontically, and to compare the thickness of the remaining tooth structure from the center of t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.007
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effects of endodontic irrigants on the push-out strength and hydration behavior of accelerated mineral trioxide aggregate (MTA) in its early setting phase. METHODS:In an in vitro perforation model, MTA with or without 10% CaCl(2) was condensed and allowed to i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.039
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:Root canal therapy is a highly successful in-office treatment and preventive measure against periapical abscesses. Left untreated, periapical abscesses can have serious consequences that can lead to hospitalization. This study observes the trends of hospitalizations attributed to periapical abscesses. MET...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.042
更新日期：2013-09-01 00:00:00
abstract：:A previous study showed that the ferret canine is an appropriate model for inducing periapical lesions. Ferret canines were used in this study to study changes in periapical lesions after root canal treatment (RCT). After periapical pathosis was induced in the maxillary and mandibular canines of six ferrets, RCT was p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80506-6
更新日期：1993-03-01 00:00:00
abstract：:The purpose of this study was to compare intracanal bacterial reduction on teeth instrumented with 0.04 tapered nickel-titanium (NiTi) rotary instrumentation to bacterial reduction when using a stainless-steel K-file step-back technique using sterile saline irrigation. Forty-eight patients with apical periodontitis we...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/S0099-2399(98)80170-2
更新日期：1998-11-01 00:00:00
abstract：INTRODUCTION:There are no long-term, prospective clinical studies assessing outcomes of endodontically treated cracked teeth with radicular extensions. The purpose of this prospective study was to examine the 2- to 4-year success and survival rates of endodontically treated, coronally restored, cracked teeth, specifica...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.015
更新日期：2019-07-01 00:00:00
abstract：:The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：:Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2014.01.017
更新日期：2014-04-01 00:00:00
abstract：:A nested polymerase chain reaction (PCR)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of Pseudoramibacter alactolyticus. Identification by nested PCR was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acut...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200311000-00012
更新日期：2003-11-01 00:00:00
abstract：INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.07.007
更新日期：2012-11-01 00:00:00
abstract：:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00004
更新日期：2004-03-01 00:00:00
abstract：INTRODUCTION:Dental pulp has limited capability to regenerate, which happens in the early stage of pulpitis. An ambiguous relationship exists; inflammation may impair or support pulp regeneration. Epigenetics, which is involved in cell proliferation and inflammation, could regulate human dental pulp cell (HDPCs) regene...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.01.031
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.09.008
更新日期：2019-12-01 00:00:00
abstract：INTRODUCTION:In regenerative endodontic treatment (RET) for immature permanent tooth, better treatment results could be obtained by applying platelet-rich plasma (PRP) as the scaffold rather than the blood clot. The goal of this study was to compare the histologic differences between using PRP and blood clot in RET. M...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.03.020
更新日期：2014-09-01 00:00:00
abstract：:The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.021
更新日期：2007-08-01 00:00:00
abstract：:A laboratory study was performed on Gates Glidden and Peeso drills to determine the bending characteristics and related metallurgical properties. Sizes #1 to #6 stainless steel Gates Glidden drills, sizes #1 to #6 stainless steel and carbon steel type P Peeso drills, and sizes #009 to #023 carbon steel-type B-1 Peeso ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80530-3
更新日期：1993-09-01 00:00:00
abstract：:A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.11.004
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three new candidate oral pathogens: Prevotella baroniae, Prevotella multisaccharivorax, and as-yet-uncultivated Bacteroidetes oral clone X083 in p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.05.033
更新日期：2009-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare, clinically and radiographically, the effects of platelet-rich fibrin (PRF) and mineral trioxide aggregate (MTA) as pulpotomy agents in permanent teeth with incomplete root development. METHODS:A total number of 70 children requiring pulpotomy in 70 pe...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2014.01.009
更新日期：2014-05-01 00:00:00
abstract：:This in vitro study evaluated the survival rate and fracture resistance of maxillary central incisors restored with different post and core systems. The post and core systems investigated were a prefabricated high precious metal post with cast core (group A), zirconia post with a prefabricated bonded ceramic core (gro...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1097/00004770-200210000-00008
更新日期：2002-10-01 00:00:00
abstract：INTRODUCTION:Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.03.006
更新日期：2018-06-01 00:00:00
abstract：:Microbial control--fundamental to healing of apical periodontitis--is central to endodontic practice. The effectiveness of antibacterial measures is generally monitored (in clinical research studies) by microbiological root canal sampling (MRS), which is often used as a predictor for healing. This article addresses th...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2006.11.006
更新日期：2007-03-01 00:00:00
abstract：:Regulated cell death (RCD) is a preferred term inclusive of all modes of cell death regulated by multiple intracellular signal transduction pathways under physiological and pathologic conditions. Although cell death programs ensure correct growth and developmental processes as well as protect the host against microbia...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2020.07.006
更新日期：2020-10-01 00:00:00
