Geographical differences in bacteria detected in endodontic infections using polymerase chain reaction.

Abstract:

:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locations. Microbial samples from abscesses of endodontic origin were collected from patients in Portland, Oregon, and Rio de Janeiro, Brazil. PCRs with species-specific oligonucleotide primers for the 16S ribosomal RNA gene were used for detection of the bacteria after DNA extraction from each clinical sample. Statistical analysis revealed that there was a significant difference in detection of the bacteria between the two geographical locations for Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Fusobacterium nucleatum, and Porphyromonas gingivalis, but not for Porphyromonas endodontalis, Fusobacterium necrophorum, and Enterococcus faecalis. These results suggest that differences in bacteria detected or cultured in studies can be associated with geographical location.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Baumgartner JC,Siqueira JF Jr,Xia T,Róças IN

doi

10.1097/00004770-200403000-00004

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

141-4

issue

3

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60307-X

journal_volume

30

pub_type

杂志文章
  • Micro-computed Tomographic Evaluation of the Shaping Ability of 3 Reciprocating Single-File Nickel-Titanium Systems on Single- and Double-Curved Root Canals.

    abstract:INTRODUCTION:We performed a micro-computed tomographic assessment of the preparation of moderately single- and double-curved root canals using 3 single-file reciprocating nickel-titanium systems: S1 Plus Standard (Sendoline, Täby, Sweden), WaveOne Gold Primary (Dentsply Sirona, Ballaigues, Switzerland), and Reciproc R2...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.05.005

    authors: Haupt F,Pult JRW,Hülsmann M

    更新日期:2020-08-01 00:00:00

  • Examining the Sensitivity and Specificity of 2 Screening Instruments: Odontogenic or Temporomandibular Disorder Pain?

    abstract:INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2016.10.001

    authors: Fonseca Alonso B,Nixdorf DR,Shueb SS,John MT,Law AS,Durham J

    更新日期:2017-01-01 00:00:00

  • Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis.

    abstract::EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200112000-00007

    authors: Sceiza MF,Daniel RL,Santos EM,Jaeger MM

    更新日期:2001-12-01 00:00:00

  • Apical extrusion of thermoplasticized obturating material in canals instrumented with Profile 0.06 or Profile GT.

    abstract::The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200406000-00010

    authors: Robinson MJ,McDonald NJ,Mullally PJ

    更新日期:2004-06-01 00:00:00

  • Success and Survival of Endodontically Treated Cracked Teeth with Radicular Extensions: A 2- to 4-year Prospective Cohort.

    abstract:INTRODUCTION:There are no long-term, prospective clinical studies assessing outcomes of endodontically treated cracked teeth with radicular extensions. The purpose of this prospective study was to examine the 2- to 4-year success and survival rates of endodontically treated, coronally restored, cracked teeth, specifica...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.03.015

    authors: Davis MC,Shariff SS

    更新日期:2019-07-01 00:00:00

  • Predictors of root canal treatment in teeth with full coverage restorations.

    abstract::This case-control study sought to identify variables predictive of subsequent root canal therapy (RCT) in teeth receiving full coverage restorations. The University of North Carolina School of Dentistry's computerized treatment database was used to identify all patients receiving a single-unit crown on a nonendodontic...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2005.11.001

    authors: Kirakozova A,Caplan DJ

    更新日期:2006-08-01 00:00:00

  • Direct effect of intracanal medicaments on survival of stem cells of the apical papilla.

    abstract:INTRODUCTION:Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH](2)) are used for disinfection. However, their effect on human stem c...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2012.06.018

    authors: Ruparel NB,Teixeira FB,Ferraz CC,Diogenes A

    更新日期:2012-10-01 00:00:00

  • Guided Endodontic Access in Maxillary Molars Using Cone-beam Computed Tomography and Computer-aided Design/Computer-aided Manufacturing System: A Case Report.

    abstract::The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2018.02.009

    authors: Lara-Mendes STO,Barbosa CFM,Santa-Rosa CC,Machado VC

    更新日期:2018-05-01 00:00:00

  • Migration of a Streptococcus sanguis strain through the root dentinal tubules.

    abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80460-7

    authors: Perez F,Calas P,de Falguerolles A,Maurette A

    更新日期:1993-06-01 00:00:00

  • Regenerative capacity of human dental pulp and apical papilla cells after treatment with a 3-antibiotic mixture.

    abstract:INTRODUCTION:A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 μg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capac...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.09.027

    authors: Phumpatrakom P,Srisuwan T

    更新日期:2014-03-01 00:00:00

  • Prevalence of Candida Species in Endodontic Infections: Systematic Review and Meta-analysis.

    abstract:INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.joen.2018.07.016

    authors: Mergoni G,Percudani D,Lodi G,Bertani P,Manfredi M

    更新日期:2018-11-01 00:00:00

  • Pulp tissue reactions to a dentin bonding agent as a direct capping agent.

    abstract::The aim of this study was to investigate the response of human pulp tissue to a dentin bonding agent, Scotchbond Multi-Purpose Plus (SMPP), in exposed class V cavities. Sixteen human premolar teeth were mechanically exposed. Ten pulps were capped with SMPP and six teeth were capped with Dycal. The cavities were filled...

    journal_title:Journal of endodontics

    pub_type: 临床试验,杂志文章

    doi:10.1097/01.don.0000137649.24821.91

    authors: Sübay RK,Demirci M

    更新日期:2005-03-01 00:00:00

  • Canal Transportation and Centering Ability in Long Oval Canals: A Multidimentional Analysis.

    abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the centering ability and canal transportation of the ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland) and Self-adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) systems in long oval root canals using cone-beam computed tomography imag...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.07.007

    authors: Nathani TI,Nathani AI,Pawar AM,Khakiani MI,Ruiz XF,Olivieri JG

    更新日期:2019-10-01 00:00:00

  • A prospective, randomized single-blind evaluation of effect of injection speed on anesthetic efficacy of inferior alveolar nerve block in patients with symptomatic irreversible pulpitis.

    abstract:INTRODUCTION:Speed of injection may affect the solution spread in the pterygomandibular space. It was hypothesized that speed of injection will affect the anesthetic efficacy of inferior alveolar nerve block (IANB) in patients with symptomatic irreversible pulpitis. METHODS:Fifty-nine adult volunteers who were activel...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2012.08.006

    authors: Aggarwal V,Singla M,Miglani S,Kohli S,Irfan M

    更新日期:2012-12-01 00:00:00

  • Root surface temperatures produced during post space preparation.

    abstract::In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(96)80264-0

    authors: Weller RN,Kimbrough WF,Anderson RW

    更新日期:1996-06-01 00:00:00

  • The use of cone-beam computed tomography and digital periapical radiographs to diagnose root perforations.

    abstract:INTRODUCTION:The aim of this study was to compare the sensitivity and specificity of cone-beam computed tomography (CBCT) scans and digital periapical radiographs (PR) in detecting strip and root perforations after root canal treatment in mandibular molars. METHODS:Forty-five curved mesial roots were endodontically pr...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2010.12.003

    authors: Shemesh H,Cristescu RC,Wesselink PR,Wu MK

    更新日期:2011-04-01 00:00:00

  • Bleaching effect of sodium percarbonate on discolored pulpless teeth in vitro.

    abstract::The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolars were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleach...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200001000-00006

    authors: Kaneko J,Inoue S,Kawakami S,Sano H

    更新日期:2000-01-01 00:00:00

  • The effect of antibiotics and endodontic antimicrobials on the polymerase chain reaction.

    abstract::The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000152899.54247.4e

    authors: Fouad AF,Barry J

    更新日期:2005-07-01 00:00:00

  • Apical anatomy in mesial and mesiobuccal roots of permanent first molars.

    abstract::The purpose of this in vitro study was to investigate the canal configuration types, and the prevalence and location of anatomical variations in the mesiobuccal (MB) and mesial roots of permanent maxillary and mandibular first molars after instrumentation. The number and the type of canals were determined before instr...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000145425.73364.91

    authors: Jung IY,Seo MA,Fouad AF,Spångberg LS,Lee SJ,Kim HJ,Kum KY

    更新日期:2005-05-01 00:00:00

  • An evaluation of the durability of apex locator insulated probes after autoclaving.

    abstract::The purpose of this study was to evaluate the durability of insulated probes designed to be used with the Endocater Apex Locator when put through multiple cleaning and autoclaving cycles. The manufacturer has reported that the plastic coating on the probes is reliable with normal use and sterilizable up to a maximum o...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(06)81502-5

    authors: Himel VT,Schott RN

    更新日期:1993-08-01 00:00:00

  • Growth and differentiation factor-5 regulates the growth and differentiation of human dental pulp cells.

    abstract:INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human d...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.06.003

    authors: Chang MC,Lin LD,Tseng HC,Chang BE,Chan CP,Lee SY,Chang HH,Lin PS,Tseng SK,Jeng JH

    更新日期:2013-10-01 00:00:00

  • Incidence of Pulpal Complications after Diagnosis of Vital Cracked Teeth.

    abstract:INTRODUCTION:This retrospective cohort study aimed to observe the incidence of pulpitis and necrosis in teeth with cracks that were not endodontically treated. METHODS:One hundred eighty-four patients with 199 cracked teeth that were diagnosed with reversible pulpitis and were treated from January 2010 to December 201...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.02.003

    authors: Wu S,Lew HP,Chen NN

    更新日期:2019-05-01 00:00:00

  • Anti-inflammatory and Osteogenic Effects of Calcium Silicate-based Root Canal Sealers.

    abstract:INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2018.09.006

    authors: Lee BN,Hong JU,Kim SM,Jang JH,Chang HS,Hwang YC,Hwang IN,Oh WM

    更新日期:2019-01-01 00:00:00

  • A standardized novel method to measure radiographic root changes after endodontic therapy in immature teeth.

    abstract:INTRODUCTION:Outcome studies of endodontic treatment of necrotic immature permanent teeth rely on radiographic measures as surrogates of whether the treatment achieved regeneration/revascularization/revitalization. An increase in radiographic root length and/or width is thought to result in a better long-term prognosis...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.09.025

    authors: Flake NM,Gibbs JL,Diogenes A,Hargreaves KM,Khan AA

    更新日期:2014-01-01 00:00:00

  • Antibacterial activity of 10% carbamide peroxide bleaching agents.

    abstract::The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(96)80217-2

    authors: Gurgan S,Bolay S,Alaçam R

    更新日期:1996-07-01 00:00:00

  • Recovery of Oral In Vitro Biofilms after Exposure to Peptides and Chlorhexidine.

    abstract:INTRODUCTION:This study aimed to examine the dynamic recovery of established multispecies biofilms of oral bacteria after an initial treatment by D-enantiomeric peptide DJK-5, L-enantiomeric peptide 1018, or chlorhexidine digluconate (CHX). METHODS:Oral biofilms from 2 donors were grown on collagen-coated hydroxyapati...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.11.020

    authors: Zhang T,Xia L,Wang Z,Hancock REW,Haapasalo M

    更新日期:2020-11-25 00:00:00

  • Effects of three canal sealers on bond strength of a fiber post.

    abstract:INTRODUCTION:This study evaluated the effects of three different endodontic sealers on the bond strength of a fiber post cemented with adhesive resin cement. METHODS:Forty-eight extracted maxillary central incisors were prepared with the step-back technique and randomly divided into four groups (n=12 for each group): ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2009.11.014

    authors: Demiryürek EO,Külünk S,Yüksel G,Saraç D,Bulucu B

    更新日期:2010-03-01 00:00:00

  • Efficacy of Different Cone-beam Computed Tomographic Protocols in the Identification of Mesiobuccal Canals of Maxillary First Molars: A Tomographic and Ex Vivo Study.

    abstract:INTRODUCTION:Complementary methods of anatomic diagnosis, including cone-beam computed tomographic (CBCT) imaging, may be useful in the diagnosis of mesiobuccal (MB) canals of maxillary molars. METHODS:Thirty-five maxillary first molars were subjected to ex vivo analysis and 4 CBCT protocols: i-CAT Classic (Imaging Sc...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2016.12.011

    authors: de Freitas JV,Baratto-Filho F,Coelho BS,Tomazinho FSF,Crozeta BM,de Sousa Neto MD,Gabardo MCL

    更新日期:2017-05-01 00:00:00

  • Machining efficiency of Flex-R, K-Flex, Trio-Cut, and S Files.

    abstract::Many root canal instruments with several different designs have been introduced during recent years. This article evaluates the machining properties of four such instruments: the Flex-R, K-Flex, Trio-Cut, and S file. There are large variations in the machining properties of these instruments. The results are comparabl...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(07)80200-7

    authors: Stenman E,Spångberg LS

    更新日期:1990-12-01 00:00:00

  • Tumor Necrosis Factor Alpha Stimulates Proliferation of Dental Pulp Stem Cells via Akt/Glycogen Synthase Kinase-3β/Cyclin D1 Signaling Pathway.

    abstract:INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.02.020

    authors: Qin Z,Li Y,Li Y,Liu G

    更新日期:2015-07-01 00:00:00