Abstract:
:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locations. Microbial samples from abscesses of endodontic origin were collected from patients in Portland, Oregon, and Rio de Janeiro, Brazil. PCRs with species-specific oligonucleotide primers for the 16S ribosomal RNA gene were used for detection of the bacteria after DNA extraction from each clinical sample. Statistical analysis revealed that there was a significant difference in detection of the bacteria between the two geographical locations for Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Fusobacterium nucleatum, and Porphyromonas gingivalis, but not for Porphyromonas endodontalis, Fusobacterium necrophorum, and Enterococcus faecalis. These results suggest that differences in bacteria detected or cultured in studies can be associated with geographical location.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Baumgartner JC,Siqueira JF Jr,Xia T,Róças INdoi
10.1097/00004770-200403000-00004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
141-4issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(05)60307-Xjournal_volume
30pub_type
杂志文章abstract:INTRODUCTION:We performed a micro-computed tomographic assessment of the preparation of moderately single- and double-curved root canals using 3 single-file reciprocating nickel-titanium systems: S1 Plus Standard (Sendoline, Täby, Sweden), WaveOne Gold Primary (Dentsply Sirona, Ballaigues, Switzerland), and Reciproc R2...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.05.005
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.001
更新日期:2017-01-01 00:00:00
abstract::EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200112000-00007
更新日期:2001-12-01 00:00:00
abstract::The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200406000-00010
更新日期:2004-06-01 00:00:00
abstract:INTRODUCTION:There are no long-term, prospective clinical studies assessing outcomes of endodontically treated cracked teeth with radicular extensions. The purpose of this prospective study was to examine the 2- to 4-year success and survival rates of endodontically treated, coronally restored, cracked teeth, specifica...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.015
更新日期:2019-07-01 00:00:00
abstract::This case-control study sought to identify variables predictive of subsequent root canal therapy (RCT) in teeth receiving full coverage restorations. The University of North Carolina School of Dentistry's computerized treatment database was used to identify all patients receiving a single-unit crown on a nonendodontic...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.11.001
更新日期:2006-08-01 00:00:00
abstract:INTRODUCTION:Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH](2)) are used for disinfection. However, their effect on human stem c...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.06.018
更新日期:2012-10-01 00:00:00
abstract::The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.009
更新日期:2018-05-01 00:00:00
abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80460-7
更新日期:1993-06-01 00:00:00
abstract:INTRODUCTION:A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 μg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capac...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.027
更新日期:2014-03-01 00:00:00
abstract:INTRODUCTION:Candida in endodontic infections has been investigated in a large number of studies, but its role as an endodontic pathogen is still debatable. The aim of this study was to systematically review the literature on the prevalence of Candida species in root canal infections. METHODS:Extensive literature rese...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.07.016
更新日期:2018-11-01 00:00:00
abstract::The aim of this study was to investigate the response of human pulp tissue to a dentin bonding agent, Scotchbond Multi-Purpose Plus (SMPP), in exposed class V cavities. Sixteen human premolar teeth were mechanically exposed. Ten pulps were capped with SMPP and six teeth were capped with Dycal. The cavities were filled...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章
doi:10.1097/01.don.0000137649.24821.91
更新日期:2005-03-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the centering ability and canal transportation of the ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland) and Self-adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) systems in long oval root canals using cone-beam computed tomography imag...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.07.007
更新日期:2019-10-01 00:00:00
abstract:INTRODUCTION:Speed of injection may affect the solution spread in the pterygomandibular space. It was hypothesized that speed of injection will affect the anesthetic efficacy of inferior alveolar nerve block (IANB) in patients with symptomatic irreversible pulpitis. METHODS:Fifty-nine adult volunteers who were activel...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.08.006
更新日期:2012-12-01 00:00:00
abstract::In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80264-0
更新日期:1996-06-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the sensitivity and specificity of cone-beam computed tomography (CBCT) scans and digital periapical radiographs (PR) in detecting strip and root perforations after root canal treatment in mandibular molars. METHODS:Forty-five curved mesial roots were endodontically pr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.12.003
更新日期:2011-04-01 00:00:00
abstract::The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolars were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleach...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200001000-00006
更新日期:2000-01-01 00:00:00
abstract::The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000152899.54247.4e
更新日期:2005-07-01 00:00:00
abstract::The purpose of this in vitro study was to investigate the canal configuration types, and the prevalence and location of anatomical variations in the mesiobuccal (MB) and mesial roots of permanent maxillary and mandibular first molars after instrumentation. The number and the type of canals were determined before instr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000145425.73364.91
更新日期:2005-05-01 00:00:00
abstract::The purpose of this study was to evaluate the durability of insulated probes designed to be used with the Endocater Apex Locator when put through multiple cleaning and autoclaving cycles. The manufacturer has reported that the plastic coating on the probes is reliable with normal use and sterilizable up to a maximum o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81502-5
更新日期:1993-08-01 00:00:00
abstract:INTRODUCTION:Growth and differentiation factor-5 (GDF-5) is a multifunctional protein that regulates the development and repair in many tissues. The purpose of this study was to investigate whether GDF-5 may influence the proliferation, differentiation, and collagen turnover of human dental pulp cells. METHODS:Human d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.06.003
更新日期:2013-10-01 00:00:00
abstract:INTRODUCTION:This retrospective cohort study aimed to observe the incidence of pulpitis and necrosis in teeth with cracks that were not endodontically treated. METHODS:One hundred eighty-four patients with 199 cracked teeth that were diagnosed with reversible pulpitis and were treated from January 2010 to December 201...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.02.003
更新日期:2019-05-01 00:00:00
abstract:INTRODUCTION:An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells. METHODS:A...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.006
更新日期:2019-01-01 00:00:00
abstract:INTRODUCTION:Outcome studies of endodontic treatment of necrotic immature permanent teeth rely on radiographic measures as surrogates of whether the treatment achieved regeneration/revascularization/revitalization. An increase in radiographic root length and/or width is thought to result in a better long-term prognosis...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.09.025
更新日期:2014-01-01 00:00:00
abstract::The antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (Nite White, Karisma, and Opalescence) on Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus casei, and Lactobacillus acidophilus was examined. Chlorhexidine (0.2%) was used as a positive control. Test m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80217-2
更新日期:1996-07-01 00:00:00
abstract:INTRODUCTION:This study aimed to examine the dynamic recovery of established multispecies biofilms of oral bacteria after an initial treatment by D-enantiomeric peptide DJK-5, L-enantiomeric peptide 1018, or chlorhexidine digluconate (CHX). METHODS:Oral biofilms from 2 donors were grown on collagen-coated hydroxyapati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.11.020
更新日期:2020-11-25 00:00:00
abstract:INTRODUCTION:This study evaluated the effects of three different endodontic sealers on the bond strength of a fiber post cemented with adhesive resin cement. METHODS:Forty-eight extracted maxillary central incisors were prepared with the step-back technique and randomly divided into four groups (n=12 for each group): ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.014
更新日期:2010-03-01 00:00:00
abstract:INTRODUCTION:Complementary methods of anatomic diagnosis, including cone-beam computed tomographic (CBCT) imaging, may be useful in the diagnosis of mesiobuccal (MB) canals of maxillary molars. METHODS:Thirty-five maxillary first molars were subjected to ex vivo analysis and 4 CBCT protocols: i-CAT Classic (Imaging Sc...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.12.011
更新日期:2017-05-01 00:00:00
abstract::Many root canal instruments with several different designs have been introduced during recent years. This article evaluates the machining properties of four such instruments: the Flex-R, K-Flex, Trio-Cut, and S file. There are large variations in the machining properties of these instruments. The results are comparabl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(07)80200-7
更新日期:1990-12-01 00:00:00
abstract:INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.02.020
更新日期:2015-07-01 00:00:00