Abstract：
INTRODUCTION:Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migration, and differentiation of human stem cells of the apical papilla (SCAPs). METHODS:CGF- and PRF-conditioned medium were prepared using the freeze-dried method. SCAPs were isolated and identified. The proliferative potential of SCAPs was investigated using the Cell Counting Kit-8 (KeyGen Biotech, Nanjing, China). The migration capacity was analyzed using transwell assays, and the mineralization ability was determined by alizarin red S staining. The expression levels of alkaline phosphatase, bone sialoprotein, dentin matrix protein 1, and dentin sialophosphoprotein were determined by quantitative polymerase chain reaction. RESULTS:The cultured cells exhibited mesenchymal stem cell characteristics. The growth rate and migratory cell numbers of the CGF and PRF groups were significantly greater than those of the control group. The mineralized areas in the CGF and PRF groups were significantly larger than those in the control group after incubation for 7 days and 14 days. The expression levels of osteogenic/odontoblast-related genes were reduced on day 7, but they were dramatically enhanced on day 14, and the related gene expression levels in the PRF group were higher than those in the CGF group. CONCLUSIONS:Both CGF and PRF can promote the proliferation, migration, and differentiation of SCAPs. CGF may be a promising alternative in regenerative endodontics.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Hong S,Chen W,Jiang Bdoi
10.1016/j.joen.2018.03.006subject
Has Abstractpub_date
2018-06-01 00:00:00pages
977-983issue
6eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30168-7journal_volume
44pub_type
杂志文章abstract：:The cytotoxic effects of a new epoxy resin-based root canal sealer (AH-plus), together with those of two other commonly used endodontic sealers (AH26 and zinc oxide-eugenol), have been studied in vitro on a culture of human gingival fibroblasts. Cytotoxicity was assessed by direct incubation of sealers' extracts with ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200008000-00008
更新日期：2000-08-01 00:00:00
abstract：:It is known that many factors participate in the process of inflammation and pain transmission. Recently, various neuropeptides have been demonstrated in the intrapulpal nerve fibers immunobiohistochemically. These neuropeptides may play some role in the process of inflammation and transmission of pain sensation. In t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81566-9
更新日期：1990-02-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effects of endodontic irrigants on the push-out strength and hydration behavior of accelerated mineral trioxide aggregate (MTA) in its early setting phase. METHODS:In an in vitro perforation model, MTA with or without 10% CaCl(2) was condensed and allowed to i...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.039
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:Apical surgery is frequently indicated in maxillary first molars. Occasionally, a vascular anastomosis in the lateral maxillary sinus wall can be observed during surgery. The aim of this study was to examine the distance between the vascular bone channel (VBC) and the root apices of maxillary first molars ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.09.004
更新日期：2019-12-01 00:00:00
abstract：:Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200301000-00013
更新日期：2003-01-01 00:00:00
abstract：INTRODUCTION:The aim of this in vitro study was to compare the effectiveness of saline, 2.5% sodium hypochlorite, and 2% chlorhexidine, with or without passive ultrasonic irrigation (PUI), in debris removal from simulated canal irregularities within prepared root canals. METHODS:Ninety bovine lateral incisors were ran...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.08.006
更新日期：2014-12-01 00:00:00
abstract：:The aim of this study was to determine the relationship between histologic and radiologic signs of inflammation in human root-filled teeth. In addition, other factors with possible importance for apical inflammation were assessed. Fifty-three block sections of root-filled teeth were gathered from human cadavers. The b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200402000-00003
更新日期：2004-02-01 00:00:00
abstract：INTRODUCTION:The aim of the present study was to compare the immunoexpression of CD34, intercellular adhesion molecule-1 (ICAM-1), and podoplanin and the presence of mast cells with clinical, demographic, radiologic, and histologic features from periapical granulomas, periapical cysts, and residual cysts. METHODS:Thir...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.04.013
更新日期：2018-07-01 00:00:00
abstract：:Vascular casts of cat premolar pulps in various stages of growth were made by injection of low-viscosity resin into the pulpal blood vessels. Examination under a scanning electron microscope revealed that, in the young pulp, the main arterioles ascend in the center of the pulp and venules run along the side of the roo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(89)80028-7
更新日期：1989-10-01 00:00:00
abstract：:This in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of Enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adheren...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.07.035
更新日期：2007-12-01 00:00:00
abstract：:In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(96)80285-8
更新日期：1996-03-01 00:00:00
abstract：:In the rat, perforations of maxillary molars were created and treated with either tricalcium phosphate (Synthograft) or Cavit. At four time intervals (1 day, 1 wk, 2 wk, and 1 month), four evaluative factors (inflammation, bone resorption, cementum and dentin resorption, and epithelial proliferation) were analyzed and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(89)80171-2
更新日期：1989-09-01 00:00:00
abstract：:Seventy-three teeth with acute irreversible pulpitis were subjected to emergency pulpotomies. After removal of the coronal portion of the pulp, a sterile cotton pellet or zinc oxide-eugenol cement was placed against the remaining pulp tissue. The cotton pellet was either dry or moistened with camphorated phenol, cresa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80219-5
更新日期：1989-06-01 00:00:00
abstract：INTRODUCTION:This study aimed to compare the physicochemical and biological properties of premixed calcium silicate-based endodontic sealers with other conventional root canal filling materials by systematically reviewing laboratory studies. METHODS:The search was conducted in 3 databases (Medline via PubMed, Scopus, ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2016.11.019
更新日期：2017-04-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the accuracy in vivo of 2 electronic apex locators (EALs), the Raypex 5 and the Mini Apex Locator. METHODS:The working length (WL) was determined electronically for 40 human root canals by using a K-file and 1 of the 2 EALs. The files were fixed at the WL, and the ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.06.021
更新日期：2011-10-01 00:00:00
abstract：:The purpose of this study was to compare the degree of anesthesia obtained with 2% lidocaine with three different concentrations of epinephrine for inferior alveolar nerve block. Using a repeated measures design, 30 subjects randomly received an inferior alveolar injection using masked cartridges of each solution at t...
journal_title：Journal of endodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/S0099-2399(97)80271-3
更新日期：1997-03-01 00:00:00
abstract：:The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.02.009
更新日期：2018-05-01 00:00:00
abstract：:The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.03.021
更新日期：2007-08-01 00:00:00
abstract：:This case report describes the clinical, radiologic, and histopathologic features of the glandular odontogenic cyst (GOC). Although a relatively rare lesion not previously reported in the endodontic literature, the GOC is recommended for inclusion in a differential diagnosis of a dentoalveolar radiolucency. The GOC is...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00014
更新日期：2004-03-01 00:00:00
abstract：:Endodontic microsurgery has reduced the degree of treatment trauma compared with traditional apical surgery and further increased the success rate of natural teeth retention. However, when root apices of the mandibular posterior teeth are far from the buccal cortical bone surface or near to the inferior alveolar nerve...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.07.033
更新日期：2020-08-11 00:00:00
abstract：INTRODUCTION:The effect of rheumatoid arthritis (RA) on the healing and pathophyisiology of apical periodontitis is unknown because there is no study in the literature evaluating the association of RA with the prevalence of periapical rarefying osteitis (PAR) and endodontic treatment. Therefore, the goal of this cross-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.015
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.07.007
更新日期：2012-11-01 00:00:00
abstract：INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.05.008
更新日期：2020-08-01 00:00:00
abstract：:The choice of one-visit versus two-visit root canal therapy for necrotic teeth with apical periodontitis is a source of current debate. The primary objective of this randomized controlled clinical trial was to compare radiographic evidence of periapical healing after root canal therapy completed in one visit or two vi...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.12.015
更新日期：2008-03-01 00:00:00
abstract：INTRODUCTION:The inferior alveolar nerve (IAN) block does not always result in successful pulpal anesthesia. Anesthetic success rates might be affected by increased anxiety. Nitrous oxide has been shown to have both anxiolytic and analgesic properties. Therefore, the purpose of this prospective, randomized, double-blin...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2012.02.010
更新日期：2012-05-01 00:00:00
abstract：:Sixty-eight standardized human root specimens were infected with Enterococcus faecalis for 3 wk after removal of the smear layer. After 3 wk of infection, the smear layer was reformed and in half of the specimens, the smear layer was again removed. Aqueous Ca(OH)2 paste and silicone oil based Ca(OH)2 paste were used a...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200105000-00004
更新日期：2001-05-01 00:00:00
abstract：:The aim of this in vivo study was to find a way to conduct qualitative and quantitative evaluations of radicular apical third apposition in rats. Twenty rats were injected intraperitoneally with tetracycline at weight-adjusted doses. Mesio-distal sections of the first mandibular molar regions were prepared. The 60 to ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80153-8
更新日期：1999-04-01 00:00:00
abstract：INTRODUCTION:This review aimed to find the most effective oral premedication in reducing pain in adults after nonsurgical root canal therapy (NSRCT) using network meta-analysis. METHODS:The review protocol was registered in the PROSPERO database (CRD42017071899). A literature search was performed in the MEDLINE and EB...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析
doi：10.1016/j.joen.2018.10.016
更新日期：2019-04-01 00:00:00
abstract：:The aim of this study was to assess the cytotoxicity of pure eugenol in an in vitro method by diluting it to various concentrations in alcohol and determining the maximum noncytotoxic concentration. We used solutions of eugenol and ethyl alcohol that are soluble in water in any given proportion. The cytotoxicity of th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80012-4
更新日期：1996-10-01 00:00:00
abstract：INTRODUCTION:Different single-file systems are available for endodontic treatment; however, comparative studies are scarce. Thus, the present study evaluated bacterial reduction promoted by 2 single-file systems: Wave One (Dentsply Maillefer, Ballaigues, Switzerland) and One Shape (Micromega, Besancon, France). METHOD...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.024
更新日期：2014-12-01 00:00:00
