Interleukin-10 receptor expression in human dental pulp cells in response to lipopolysaccharide from Prevotella intermedia.

Abstract:

:Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. Maximal production of the IL-10R protein in dental pulp fibroblast cultures was detected by Western blot analysis after 12 h of LPS stimulation. In contrast, the human skin fibroblast (SF-MA) and human monocyte (U937) cell lines expressed IL-10R mRNA. Anti-CD14 antibodies inhibited P. intermedia LPS-induced IL-10R mRNA expression. These results indicate that P. intermedia LPS induces IL-10R gene expression in human, dental pulp fibroblasts in vitro.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Tokuda M,Nagaoka S,Torii M

doi

10.1097/00004770-200301000-00013

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

48-50

issue

1

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60795-9

journal_volume

29

pub_type

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