Abstract：
INTRODUCTION:Dental pulp has limited capability to regenerate, which happens in the early stage of pulpitis. An ambiguous relationship exists; inflammation may impair or support pulp regeneration. Epigenetics, which is involved in cell proliferation and inflammation, could regulate human dental pulp cell (HDPCs) regeneration. The aim of this study was to determine the role of the epigenetic mark, enhancer of zeste homolog 2 (EZH2), in the inflammation, proliferation, and regeneration of dental pulp. We used trimethylated histone H3 lysine 27(H3K27me3) and its lysine demethylase 6B (KDM6B) to monitor functional effects of altered EZH2 levels. METHODS:We detected epigenetic marks (EZH2, H3K27me3, and KDM6B) in pulp tissue by immunohistochemistry and immunofluorescence. EZH2 levels in HDPCs in inflammatory responses or differentiation were analyzed by quantitative polymerase chain reaction and Western blot. Quantitative polymerase chain reaction was used to assess the effects of EZH2 inhibition on interleukins in HDPCs upon tumor necrosis factor alpha stimulation. Cell proliferation was tested by cell counting kit-8, cell cycle, and apoptosis analysis. HDPC differentiation was investigated by quantitative polymerase chain reaction, alkaline phosphatase activity, and oil red O staining. RESULTS:EZH2 and H3K27me3 were decreased, whereas KDM6B was increased in infected pulp tissue and cells, which were similar to HDPC differentiation. EZH2 inhibition suppressed IL-1b, IL-6, and IL-8 messenger RNA (mRNA) in HDPCs upon inflammatory stimuli and impeded HDPC proliferation by decreasing cell number, arresting cell cycle, and increasing apoptosis. Suppressed EZH2 impaired adipogenesis, peroxisome proliferator-activated receptor r (PPAR-r), and CCAAT-enhancer binding protein a (CEBP/a) mRNA in adipogenic induction while enhancing alkaline phosphatase activity, Osx, and bone sialoprotein (BSP) mRNA in mineralization induction of HDPCs. CONCLUSIONS:EZH2 inhibited HDPC osteogenic differentiation while enhancing inflammatory response and proliferation, suggesting its role in pulp inflammation, proliferation, and regeneration.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Hui T,A P,Zhao Y,Wang C,Gao B,Zhang P,Wang J,Zhou X,Ye Ldoi
10.1016/j.joen.2014.01.031subject
Has Abstractpub_date
2014-08-01 00:00:00pages
1132-8issue
8eissn
0099-2399issn
1878-3554pii
S0099-2399(14)00092-2journal_volume
40pub_type
杂志文章abstract：INTRODUCTION:The aim of this study was to introduce an innovative method for autotransplantation of teeth using 3-dimensional (3D) surgical templates for guided osteotomy preparation and donor tooth placement. METHODS:This report describes autotransplantation of immature premolars as treatment of an 11-year-old boy ha...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.08.021
更新日期：2016-12-01 00:00:00
abstract：INTRODUCTION:Sphingosine-1-phosphate receptor 1 (S1P1) is crucial for regulation of immunity and bone metabolism. This study aimed to investigate the expression of S1P1 in rat periapical lesions and its relationship with receptor activator of nuclear factor kappa B ligand (RANKL) and regulatory T (Treg) cells. METHODS...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.10.010
更新日期：2015-04-01 00:00:00
abstract：:The antimicrobial effect of RC-Prep and its components--10% urea peroxide, 15% EDTA, and glycol--were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent fo...
journal_title：Journal of endodontics
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doi：10.1016/S0099-2399(99)80296-9
更新日期：1999-12-01 00:00:00
abstract：INTRODUCTION:Incision and drainage of symptomatic emergency patients with facial swelling is painful even after local anesthetics are administered. The purpose of this prospective, randomized, double-blind study was to compare the pain of infiltration and the pain of an incision and drainage procedure of a buffered ver...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2015.06.017
更新日期：2015-10-01 00:00:00
abstract：:This case report describes an innovative virtual simulation method using a computer-aided rapid prototyping (CARP) model and a computer-aided design (CAD) program for autotransplantation of an immature third molar.A compromised left mandibular second molar (#18) was extracted and replaced by autotransplantation using ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.08.010
更新日期：2018-12-01 00:00:00
abstract：:An in vitro, flow-through optical system was designed to measure hemoglobin (Hb) concentrations in the pulp space. The system included light-emitting diodes and a silicon photodetector positioned on opposing surfaces of human teeth. A syringe pump allowed a controlled flow of blood through the pulp chamber. The Hb con...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80230-5
更新日期：1996-01-01 00:00:00
abstract：:The aim of this in vitro study was to determine whether irrigation with apical negative pressure was more effective than traditional positive-pressure irrigation in eradicating Enterococcus faecalis from preshaped root canals. Fifty-four extracted mandibular molars were instrumented to produce either a non-tapered or ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.07.022
更新日期：2008-11-01 00:00:00
abstract：:Root canal bacteria in teeth with apical periodontitis were enumerated after extraction and incubation. Canals in 36 teeth were sampled after: S1, incubation for 2 hours (group A), 2 days (group B), 4 days (group C), and 6 days (group D); S2, subsequent incubation for 1 week; S3, canal disinfection; and S4, final incu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.038
更新日期：2008-12-01 00:00:00
abstract：INTRODUCTION:The objective of this study was to evaluate the pH and calcium ion release of calcium hydroxide pastes associated with different substances. METHODS:Forty acrylic teeth with simulated root canals were divided into 4 groups according to the substance associated to the calcium hydroxide paste: chlorhexidine...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.05.009
更新日期：2009-09-01 00:00:00
abstract：INTRODUCTION:This study evaluated the biocompatibility of 5 and 10 μg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. METHODS:Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.08.010
更新日期：2017-12-01 00:00:00
abstract：:Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2014.01.017
更新日期：2014-04-01 00:00:00
abstract：:This study compared the quality of obturation of high- and low-temperature thermoplasticized injectable gutta-percha techniques and standard lateral condensation. A new model system was developed to more closely simulate the clinical environment. All obturations were performed in this same model which allowed direct c...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81863-7
更新日期：1991-06-01 00:00:00
abstract：:A tight and long-lasting seal of root-end fillings is of prime clinical importance. A hundred standard bovine root sections, each 3 mm high and with a central pulp lumen of 2.6 mm in diameter, were filled with five commonly used or potential root-end filling materials. At 24 h, or at 3, 6, or 12 months after filling, ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80077-0
更新日期：1998-08-01 00:00:00
abstract：:Previous dye leakage studies have shown that mineral trioxide aggregate leaks significantly less than other commonly used root-end filling materials. This study determined the time needed for Staphylococcus epidermidis to penetrate a 3-mm thickness of amalgam, Super-EBA, Intermediate Restorative Material (IRM), or min...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80433-4
更新日期：1995-03-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare the shaping ability of 4 different nickel-titanium systems, considering their variation in alloy treatment, kinematics, and design, used to prepare canals with severe curvature using micro-computed tomographic analysis. METHODS:Thirty-two mesial roots of mandibular mol...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.02.018
更新日期：2019-06-01 00:00:00
abstract：BACKGROUND:The aim of this in vitro study was to evaluate the depth of penetration of 4 different endodontic resin sealers into the radicular dentinal tubules with the aid of confocal microscopy. METHODS:Eighty single-rooted teeth were instrumented and divided into 4 groups composed of 20 teeth each. The samples were ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.017
更新日期：2012-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. METHODS:After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.10.002
更新日期：2012-02-01 00:00:00
abstract：:This study was carried out to investigate the effect of changes in tooth structure due to masticatory cycles on amalgam retrofillings performed to different cavity depths. Forty upper maxillary first molars were tested. In the obturated palatal root apical amalgam was inserted to different depths (1.5 and 3 mm). Eight...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80169-0
更新日期：1997-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.034
更新日期：2017-03-01 00:00:00
abstract：INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.03.006
更新日期：2014-09-01 00:00:00
abstract：:A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title：Journal of endodontics
pub_type： 杂志文章
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更新日期：1992-04-01 00:00:00
abstract：:Ultrasonic instruments are a valuable asset for removing intraradicular posts from root canals before nonsurgical endodontic therapy. The purpose of this study was to evaluate the relative efficacy of post removal by ultrasonic and sonic devices. Fifty extracted teeth were instrumented and obturated. The canals were p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80044-0
更新日期：1994-10-01 00:00:00
abstract：:A polydimethylsiloxane-based sealer (GuttaFlow) was used for filling single-rooted teeth with four obturation techniques. Prefitted gutta-percha master-cones were employed in groups 1 to 3 with backfilling of the sealer. Apical third of the roots were first filled with GuttaFlow, and sealer-coated master cones were se...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.07.009
更新日期：2006-12-01 00:00:00
abstract：:The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80395-1
更新日期：1999-01-01 00:00:00
abstract：:The Editorial Board of the Journal of Endodontics has developed a literature-based study guide of topical areas related to endodontics. This study guide is intended to give the reader a focused review of the essential endodontic literature and does not cite all possible articles related to each topic. Although citing ...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2007.07.002
更新日期：2008-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the prevalence and characteristics of postoperative pain after endodontic microsurgery and to identify potential predictors for severe pain. METHODS:One hundred seventy-three patients who underwent endodontic microsurgery at a private practice were included in the st...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.12.012
更新日期：2020-12-23 00:00:00
abstract：INTRODUCTION:Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin. M...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.05.011
更新日期：2012-09-01 00:00:00
abstract：INTRODUCTION:This study evaluated the effects of three different endodontic sealers on the bond strength of a fiber post cemented with adhesive resin cement. METHODS:Forty-eight extracted maxillary central incisors were prepared with the step-back technique and randomly divided into four groups (n=12 for each group): ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.014
更新日期：2010-03-01 00:00:00
abstract：INTRODUCTION:Osteoporosis is a major systemic disease that can significantly deteriorate the quality of life of the affected individuals. It is more common in women, particularly after menopause. Osteoporosis may be associated with alterations in oral health. Treatment of osteoporotic patients mainly involves the admin...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.10.019
更新日期：2021-02-01 00:00:00
abstract：INTRODUCTION:The aim of this retrospective study was to evaluate the outcome of endodontic microsurgery and to examine prognostic factors related to healing. METHODS:The clinical records of all patients who had undergone endodontic microsurgery from 1997-2003 at the National Dental Centre of Singapore were examined. T...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.04.005
更新日期：2014-08-01 00:00:00
