Pseudoramibacter alactolyticus in primary endodontic infections.

Abstract:

:A nested polymerase chain reaction (PCR)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of Pseudoramibacter alactolyticus. Identification by nested PCR was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acute apical periodontitis, and in pus samples from acute periradicular abscesses. DNA was extracted from the samples and initially amplified using universal 16S rDNA primers. A second round of amplification used the first PCR products to detect a specific fragment of P. alactolyticus 16S rDNA. P. alactolyticus was detected in 76% of root-canal samples from teeth showing asymptomatic periradicular lesions, in 60% of samples taken from root canals associated with acute apical periodontitis, and in 32% of pus samples aspirated from acute periradicular abscesses. No significant association of this species with clinical symptoms was observed (p > 0.01). In general, P. alactolyticus occurred in 56% of samples taken from infections of endodontic origin. The high prevalence of P. alactolyticus in infections of endodontic origin as detected by nested PCR in this study, and its apparent pathogenicity, particularly in mixed infections, indicate that this bacterial species is a candidate endodontic pathogen that can participate in the etiology of different forms of periradicular diseases.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Siqueira JF Jr,Rôças IN

doi

10.1097/00004770-200311000-00012

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

735-8

issue

11

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(05)60364-0

journal_volume

29

pub_type

杂志文章
  • Behavior of nickel-titanium instruments manufactured with different thermal treatments.

    abstract:INTRODUCTION:The purpose of this study was to investigate if nickel-titanium instruments with similar designs manufactured by different thermal treatments would exhibit significantly different in vitro behavior. METHODS:Thirty-six instruments each of ProTaper Universal (PTU F1; Dentsply Maillefer, Ballaigues, Switzerl...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2014.06.005

    authors: Pereira ÉS,Viana AC,Buono VT,Peters OA,Bahia MG

    更新日期:2015-01-01 00:00:00

  • Endodontic and salivary isolates of Enterococcus faecalis integrate into biofilm from human salivary bacteria cultivated in vitro.

    abstract:INTRODUCTION:The aim of this study was to examine whether Enterococcus faecalis isolates from endodontic patients (from saliva and from a root canal) are able to prevail against salivary bacteria when grown in coculture in a biofilm reactor. METHODS:Saliva that was tested to be free of E. faecalis was used as the inoc...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2009.04.013

    authors: Al-Ahmad A,Müller N,Wiedmann-Al-Ahmad M,Sava I,Hübner J,Follo M,Schirrmeister J,Hellwig E

    更新日期:2009-07-01 00:00:00

  • Efficacy and Safety of Postoperative Medications in Reducing Pain after Nonsurgical Endodontic Treatment: A Systematic Review and Network Meta-analysis.

    abstract:INTRODUCTION:The purpose of this study was to evaluate the efficacy and safety of postoperative medications in decreasing pain after nonsurgical endodontic treatment using a network meta-analytic approach. METHODS:MEDLINE, Embase, CENTRAL, CINAHL, and Scopus were searched (until July 31, 2019). Two reviewers selected ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.joen.2020.07.002

    authors: Zanjir M,Sgro A,Lighvan NL,Yarascavitch C,Shah PS,da Costa BR,Azarpazhooh A

    更新日期:2020-10-01 00:00:00

  • Effect of Diclofenac Potassium Premedication on Postendodontic Pain in Mandibular Molars with Symptomatic Irreversible Pulpitis: A Randomized Placebo-Controlled Double-Blind Trial.

    abstract:INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2020.05.008

    authors: Al-Rawhani AH,Gawdat SI,Wanees Amin SA

    更新日期:2020-08-01 00:00:00

  • KLF4 promotes the odontoblastic differentiation of human dental pulp cells.

    abstract:INTRODUCTION:Krüppel-like factor 4 (KLF4) plays an important role in cytodifferentiation and proliferation. Our previous study showed that KLF4 was specifically expressed in polarizing and elongating odontoblasts. However, the role of KLF4 in odontoblast differentiation was still unknown. The purpose of this study was ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2011.03.030

    authors: Lin H,Xu L,Liu H,Sun Q,Chen Z,Yuan G,Chen Z

    更新日期:2011-07-01 00:00:00

  • Odontogenic cutaneous sinus tract.

    abstract::Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(06)80959-3

    authors: Foster KH,Primack PD,Kulild JC

    更新日期:1992-06-01 00:00:00

  • A survey of filling methods, intracanal medications, and instrument breakage.

    abstract::The present survey was conducted to obtain answers to some basic questions regarding the use of intracanal medications, root canal filling methods, and the intracanal breakage of instruments. A letter was sent to 300 endodontists listed in the 1995-1996 membership roster of the American Association of Endodontists. Ei...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(99)80306-9

    authors: Itoh A,Higuchi N,Minami G,Yasue T,Yoshida T,Maseki T,Nakamura H

    更新日期:1999-12-01 00:00:00

  • Adhesive sealing of the pulp chamber.

    abstract::The purpose of this in vitro study was to evaluate quantitatively the ability of four different filling materials to seal the orifices of root canals as a secondary seal after root canal therapy. Forty extracted human molar teeth were used. The top of pulp chambers and distal halves of the roots were removed using an ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200108000-00006

    authors: Belli S,Zhang Y,Pereira PN,Pashley DH

    更新日期:2001-08-01 00:00:00

  • A comparative study of apical leakage of Endomethasone, Top Seal, and Roeko Seal sealer cements.

    abstract::A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200206000-00001

    authors: Lucena-Martín C,Ferrer-Luque CM,González-Rodríguez MP,Robles-Gijón V,Navajas-Rodríguez de Mondelo JM

    更新日期:2002-06-01 00:00:00

  • Root surface temperatures produced during post space preparation.

    abstract::In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(96)80264-0

    authors: Weller RN,Kimbrough WF,Anderson RW

    更新日期:1996-06-01 00:00:00

  • An unusual indication for root resection.

    abstract::This report presents an unusual indication for root resection of a lower molar tooth and also reviews the history of root resection. ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,评审

    doi:10.1097/00004770-200004000-00016

    authors: Johnston DJ,Cowan CG,Hussey DL

    更新日期:2000-04-01 00:00:00

  • An immunohistochemical study of the behavior of cells expressing interleukin-1 alpha and interleukin-1 beta within experimentally induced periapical lesions in rats.

    abstract::Cells expressing interleukin-1 (IL-1) were examined in rat periapical lesions using immunohistochemical methods. During the acute phase, both IL-1 alpha- and IL-1 beta-expressing cells were observed adjacent to many osteoclasts that contained large cytoplasms, and consequently the periapical lesions tended to expand. ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(98)80008-3

    authors: Matsumoto A,Anan H,Maeda K

    更新日期:1998-12-01 00:00:00

  • A Correlation between Wnt/Beta-catenin Signaling and the Rate of Dentin Secretion.

    abstract:INTRODUCTION:Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the rate of dentin accumulation. METHODS:Histology, immunohistochemistry, vital dye labeling, and histomorphometric assays were used to quant...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.07.014

    authors: Zhao Y,Yuan X,Bellido T,Helms JA

    更新日期:2019-11-01 00:00:00

  • MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay.

    abstract::Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/01.don.0000128749.50151.24

    authors: de Leimburg ML,Angeretti A,Ceruti P,Lendini M,Pasqualini D,Berutti E

    更新日期:2004-12-01 00:00:00

  • Evaluation of microsurgery with SuperEBA as root-end filling material for treating post-treatment endodontic disease: a 2-year retrospective study.

    abstract:INTRODUCTION:This retrospective study assessed the effects of microsurgical treatment of post-treatment endodontic disease using SuperEBA (Bosworth, Skokie, IL) as the root-end filling material and evaluated the potential prognostic factors in relation to outcome. METHODS:Data were collected from patients diagnosed wi...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2013.11.001

    authors: Li H,Zhai F,Zhang R,Hou B

    更新日期:2014-03-01 00:00:00

  • An in vitro evaluation of the sealing ability of a new root-canal-obturation system.

    abstract::In this study the sealing ability of a new urethane methacrylate resin-based sealer, EndoRez, was evaluated using a fluid-filtration model. Sixty-four single-rooted lower bicuspids were decoronated, instrumented, and divided into 3 groups of 20 each with 4 teeth used as controls. In group A, the roots were obturated w...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200310000-00011

    authors: Kardon BP,Kuttler S,Hardigan P,Dorn SO

    更新日期:2003-10-01 00:00:00

  • Histologic assessment of mineral trioxide aggregate as a root-end filling in monkeys.

    abstract::Mineral Trioxide Aggregate (MTA) has been shown in a number of experiments to be a potential root-end filling material. The purpose of this study was to examine the periradicular tissue response of monkeys to MTA and amalgam as root-end fillings. The pulps were removed from all the maxillary incisors of three monkeys....

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(97)80051-9

    authors: Torabinejad M,Pitt Ford TR,McKendry DJ,Abedi HR,Miller DA,Kariyawasam SP

    更新日期:1997-04-01 00:00:00

  • Nonsurgical Endodontic Management of a Molar-Incisor Malformation-affected Mandibular First Molar: A Case Report.

    abstract::A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.11.004

    authors: Yue W,Kim E

    更新日期:2016-04-01 00:00:00

  • Evaluation of a dentin barrier test by cyctotoxicity testing of various dental cements.

    abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/s0099-2399(96)80285-8

    authors: Schmalz G,Schweikl H,Esch J,Hiller KA

    更新日期:1996-03-01 00:00:00

  • Synergistic effect of microbubble emulsion and sonic or ultrasonic agitation on endodontic biofilm in vitro.

    abstract:INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2012.07.007

    authors: Halford A,Ohl CD,Azarpazhooh A,Basrani B,Friedman S,Kishen A

    更新日期:2012-11-01 00:00:00

  • Ultrastructure of smear layer-covered intraradicular dentin after irrigation with BioPure MTAD.

    abstract::The structure of mechanically instrumented intraradicular dentin after irrigation with NaOCl as the initial rinse and BioPure MTAD as the final rinse were examined from the coronal, middle, and apical parts of root canal walls using transmission electron microscopy. Sterile distilled water and EDTA as final rinses wer...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2005.10.035

    authors: Tay FR,Pashley DH,Loushine RJ,Doyle MD,Gillespie WT,Weller RN,King NM

    更新日期:2006-03-01 00:00:00

  • Comparison of the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation.

    abstract::The purpose of this study was to compare the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation. Twenty-six bilaterally matched pairs of human teeth were collected. The teeth were incubated with Enterococcus faecalis for 4 weeks. The teeth were divided into two experime...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.joen.2006.08.007

    authors: Johal S,Baumgartner JC,Marshall JG

    更新日期:2007-01-01 00:00:00

  • Evaluation of the controlled placement of injected thermoplasticized gutta-percha.

    abstract::Ninety extracted human teeth with a single canal and a large patent foramen were obturated by one of three methods after placement of a master gutta-percha cone and sealer. The apical seals were evaluated by ink penetration. Statistical analysis of the results indicated that the group obturated with the high-temperatu...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/S0099-2399(89)80052-4

    authors: Olson AK,Hartwell GR,Weller RN

    更新日期:1989-07-01 00:00:00

  • Effectiveness of ProFile .04 taper rotary instruments in endodontic retreatment.

    abstract::This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200002000-00010

    authors: Sae-Lim V,Rajamanickam I,Lim BK,Lee HL

    更新日期:2000-02-01 00:00:00

  • Guided Osteotomy and Guided Autotransplantation for Treatment of Severely Impacted Teeth: A Proof-of-Concept Report.

    abstract:INTRODUCTION:The aim was to present a novel surgical technique using virtually preplanned 3-dimensional (3D)-printed templates for guided osteotomies. These were to ensure atraumatic uncovering of a severely impacted donor tooth including guided drilling of the recipient alveolus followed by a secure autotransplantatio...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2020.07.024

    authors: Strbac GD,Schnappauf A,Bertl MH,Vasak C,Ulm C,Giannis K

    更新日期:2020-07-29 00:00:00

  • Heat Generation Changes with Electrically Heated Pluggers after Multiple Autoclave Cycles at Different Operating Temperatures.

    abstract:INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2019.09.008

    authors: Correa M Jr,Dutner JM,Phillips MB,Sidow SJ,McPherson J

    更新日期:2019-12-01 00:00:00

  • Bacterial leakage with mineral trioxide aggregate or a resin-modified glass ionomer used as a coronal barrier.

    abstract::The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1097/00004770-200411000-00008

    authors: Tselnik M,Baumgartner JC,Marshall JG

    更新日期:2004-11-01 00:00:00

  • Age-related Changes in the Alkaline Phosphatase Activity of Healthy and Inflamed Human Dental Pulp.

    abstract:INTRODUCTION:Alkaline phosphatase (ALP) plays an important role in inducing mineralization events in the dental pulp. This study investigated and compared the ALP levels in healthy and inflamed pulp in young and old human pulp. METHODS:Tissue samples were collected from young (<30 years) and old (>60 years) donors. In...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.10.003

    authors: Aslantas EE,Buzoglu HD,Karapinar SP,Cehreli ZC,Muftuoglu S,Atilla P,Aksoy Y

    更新日期:2016-01-01 00:00:00

  • Effects of sodium tri- and hexametaphosphate on proliferation, differentiation, and angiogenic potential of human dental pulp cells.

    abstract:INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the prolifera...

    journal_title:Journal of endodontics

    pub_type: 杂志文章

    doi:10.1016/j.joen.2015.01.038

    authors: Bae WJ,Jue SS,Kim SY,Moon JH,Kim EC

    更新日期:2015-06-01 00:00:00

  • Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival.

    abstract::Coconut water is biologically pure and sterile, with a rich presence of amino acids, proteins, vitamins, and minerals. The purpose of this study was to use a collagenase-dispase assay to investigate the potential of a new storage medium, coconut water, in comparison with propolis, Hank's balanced salt solution (HBSS),...

    journal_title:Journal of endodontics

    pub_type: 杂志文章,收录出版

    doi:10.1016/j.joen.2008.01.018

    authors: Gopikrishna V,Baweja PS,Venkateshbabu N,Thomas T,Kandaswamy D

    更新日期:2008-05-01 00:00:00