Abstract:
:A nested polymerase chain reaction (PCR)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of Pseudoramibacter alactolyticus. Identification by nested PCR was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acute apical periodontitis, and in pus samples from acute periradicular abscesses. DNA was extracted from the samples and initially amplified using universal 16S rDNA primers. A second round of amplification used the first PCR products to detect a specific fragment of P. alactolyticus 16S rDNA. P. alactolyticus was detected in 76% of root-canal samples from teeth showing asymptomatic periradicular lesions, in 60% of samples taken from root canals associated with acute apical periodontitis, and in 32% of pus samples aspirated from acute periradicular abscesses. No significant association of this species with clinical symptoms was observed (p > 0.01). In general, P. alactolyticus occurred in 56% of samples taken from infections of endodontic origin. The high prevalence of P. alactolyticus in infections of endodontic origin as detected by nested PCR in this study, and its apparent pathogenicity, particularly in mixed infections, indicate that this bacterial species is a candidate endodontic pathogen that can participate in the etiology of different forms of periradicular diseases.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Siqueira JF Jr,Rôças INdoi
10.1097/00004770-200311000-00012keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
735-8issue
11eissn
0099-2399issn
1878-3554pii
S0099-2399(05)60364-0journal_volume
29pub_type
杂志文章abstract:INTRODUCTION:The purpose of this study was to investigate if nickel-titanium instruments with similar designs manufactured by different thermal treatments would exhibit significantly different in vitro behavior. METHODS:Thirty-six instruments each of ProTaper Universal (PTU F1; Dentsply Maillefer, Ballaigues, Switzerl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.06.005
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to examine whether Enterococcus faecalis isolates from endodontic patients (from saliva and from a root canal) are able to prevail against salivary bacteria when grown in coculture in a biofilm reactor. METHODS:Saliva that was tested to be free of E. faecalis was used as the inoc...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.04.013
更新日期:2009-07-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the efficacy and safety of postoperative medications in decreasing pain after nonsurgical endodontic treatment using a network meta-analytic approach. METHODS:MEDLINE, Embase, CENTRAL, CINAHL, and Scopus were searched (until July 31, 2019). Two reviewers selected ...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2020.07.002
更新日期:2020-10-01 00:00:00
abstract:INTRODUCTION:The aim of this prospective, randomized, double-blind clinical trial was to evaluate the effect of a preoperative, single, oral dose of diclofenac potassium (DFK) on postoperative pain and rescue analgesic intake in patients with symptomatic irreversible pulpitis in mandibular molars treated in 1 visit. M...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2020.05.008
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:Krüppel-like factor 4 (KLF4) plays an important role in cytodifferentiation and proliferation. Our previous study showed that KLF4 was specifically expressed in polarizing and elongating odontoblasts. However, the role of KLF4 in odontoblast differentiation was still unknown. The purpose of this study was ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.03.030
更新日期:2011-07-01 00:00:00
abstract::Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80959-3
更新日期:1992-06-01 00:00:00
abstract::The present survey was conducted to obtain answers to some basic questions regarding the use of intracanal medications, root canal filling methods, and the intracanal breakage of instruments. A letter was sent to 300 endodontists listed in the 1995-1996 membership roster of the American Association of Endodontists. Ei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80306-9
更新日期:1999-12-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate quantitatively the ability of four different filling materials to seal the orifices of root canals as a secondary seal after root canal therapy. Forty extracted human molar teeth were used. The top of pulp chambers and distal halves of the roots were removed using an ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200108000-00006
更新日期:2001-08-01 00:00:00
abstract::A comparison was made of the apical leakage of three sealers. Fifty single-root human teeth were randomly divided into 5 groups (n = 10; 3 experimental and 2 control). The teeth of the positive-control and experimental groups were instrumented with K-type files to size 45. The experimental groups were obturated by lat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200206000-00001
更新日期:2002-06-01 00:00:00
abstract::In vitro root surface temperatures produced during post space preparation were measured. The preparations were made using a GPX bur with rotational speeds of 6,500, 8,000, 9,500, 11,000, and 15,000 rpm. The overall mean temperature increase ranged from 0.66 degree to 4.81 degrees C and was greatest at 8,000 rpm. When ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80264-0
更新日期:1996-06-01 00:00:00
abstract::This report presents an unusual indication for root resection of a lower molar tooth and also reviews the history of root resection. ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1097/00004770-200004000-00016
更新日期:2000-04-01 00:00:00
abstract::Cells expressing interleukin-1 (IL-1) were examined in rat periapical lesions using immunohistochemical methods. During the acute phase, both IL-1 alpha- and IL-1 beta-expressing cells were observed adjacent to many osteoclasts that contained large cytoplasms, and consequently the periapical lesions tended to expand. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80008-3
更新日期:1998-12-01 00:00:00
abstract:INTRODUCTION:Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the rate of dentin accumulation. METHODS:Histology, immunohistochemistry, vital dye labeling, and histomorphometric assays were used to quant...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.07.014
更新日期:2019-11-01 00:00:00
abstract::Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000128749.50151.24
更新日期:2004-12-01 00:00:00
abstract:INTRODUCTION:This retrospective study assessed the effects of microsurgical treatment of post-treatment endodontic disease using SuperEBA (Bosworth, Skokie, IL) as the root-end filling material and evaluated the potential prognostic factors in relation to outcome. METHODS:Data were collected from patients diagnosed wi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.11.001
更新日期:2014-03-01 00:00:00
abstract::In this study the sealing ability of a new urethane methacrylate resin-based sealer, EndoRez, was evaluated using a fluid-filtration model. Sixty-four single-rooted lower bicuspids were decoronated, instrumented, and divided into 3 groups of 20 each with 4 teeth used as controls. In group A, the roots were obturated w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200310000-00011
更新日期:2003-10-01 00:00:00
abstract::Mineral Trioxide Aggregate (MTA) has been shown in a number of experiments to be a potential root-end filling material. The purpose of this study was to examine the periradicular tissue response of monkeys to MTA and amalgam as root-end fillings. The pulps were removed from all the maxillary incisors of three monkeys....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(97)80051-9
更新日期:1997-04-01 00:00:00
abstract::A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.11.004
更新日期:2016-04-01 00:00:00
abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(96)80285-8
更新日期:1996-03-01 00:00:00
abstract:INTRODUCTION:Irrigation dynamics and antibacterial activity determine the efficacy of root canal disinfection. Sonic or ultrasonic agitation of irrigants is expected to improve irrigation dynamics. This study examined the effects of microbubble emulsion (ME) combined with sonic or ultrasonic agitation on irrigation dyn...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.07.007
更新日期:2012-11-01 00:00:00
abstract::The structure of mechanically instrumented intraradicular dentin after irrigation with NaOCl as the initial rinse and BioPure MTAD as the final rinse were examined from the coronal, middle, and apical parts of root canal walls using transmission electron microscopy. Sterile distilled water and EDTA as final rinses wer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.10.035
更新日期:2006-03-01 00:00:00
abstract::The purpose of this study was to compare the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation. Twenty-six bilaterally matched pairs of human teeth were collected. The teeth were incubated with Enterococcus faecalis for 4 weeks. The teeth were divided into two experime...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2006.08.007
更新日期:2007-01-01 00:00:00
abstract::Ninety extracted human teeth with a single canal and a large patent foramen were obturated by one of three methods after placement of a master gutta-percha cone and sealer. The apical seals were evaluated by ink penetration. Statistical analysis of the results indicated that the group obturated with the high-temperatu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80052-4
更新日期:1989-07-01 00:00:00
abstract::This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200002000-00010
更新日期:2000-02-01 00:00:00
abstract:INTRODUCTION:The aim was to present a novel surgical technique using virtually preplanned 3-dimensional (3D)-printed templates for guided osteotomies. These were to ensure atraumatic uncovering of a severely impacted donor tooth including guided drilling of the recipient alveolus followed by a secure autotransplantatio...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.07.024
更新日期:2020-07-29 00:00:00
abstract:INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.09.008
更新日期:2019-12-01 00:00:00
abstract::The purpose of this study was to evaluate gray mineral trioxide aggregate (MTA), white MTA, and Fuji II LC cement as coronal barriers to bacterial leakage. Seventy-eight, matched, human teeth were obturated with gutta-percha. In group I, 18 pairs received a 3-mm barrier of gray or white MTA. In group II, 18 pairs rece...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200411000-00008
更新日期:2004-11-01 00:00:00
abstract:INTRODUCTION:Alkaline phosphatase (ALP) plays an important role in inducing mineralization events in the dental pulp. This study investigated and compared the ALP levels in healthy and inflamed pulp in young and old human pulp. METHODS:Tissue samples were collected from young (<30 years) and old (>60 years) donors. In...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.10.003
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:This study aimed to investigate the effects of 2 inorganic polyphosphates (poly[P]) are linear polymers of orthophosphate (Pi) residues linked by energy-rich phosphoanhydride poly(P) compounds, sodium triphosphate (STP, Na5P3O10) and sodium hexametaphosphate (SHMP, Na15P13O40 ∼ Na20P18O40) on the prolifera...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.01.038
更新日期:2015-06-01 00:00:00
abstract::Coconut water is biologically pure and sterile, with a rich presence of amino acids, proteins, vitamins, and minerals. The purpose of this study was to use a collagenase-dispase assay to investigate the potential of a new storage medium, coconut water, in comparison with propolis, Hank's balanced salt solution (HBSS),...
journal_title:Journal of endodontics
pub_type: 杂志文章,收录出版
doi:10.1016/j.joen.2008.01.018
更新日期:2008-05-01 00:00:00