Abstract：
INTRODUCTION:Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three new candidate oral pathogens: Prevotella baroniae, Prevotella multisaccharivorax, and as-yet-uncultivated Bacteroidetes oral clone X083 in primary endodontic infections using a devised culture-independent approach. METHODS:Genomic DNA was isolated from samples taken from 52 teeth with different forms of apical periodontitis and used as template in a taxon-specific 16S rRNA gene-based nested polymerase chain reaction assay to determine the prevalence of the 3 target taxa. RESULTS:Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were respectively detected in 81%, 43%, and 38% of the root canals of teeth associated with chronic apical periodontitis, in 60%, 40% and 40% of the canals of teeth with acute apical periodontitis, and in 14%, 24%, and 5% of the pus aspirates from acute apical abscesses. No targeted taxon was positively associated with abscesses or clinical symptoms. Overall, Bacteroidetes clone X083, P. baroniae, and P. multisaccharivorax were found in 50%, 35%, and 25% of the samples taken from primary endodontic infections, respectively. CONCLUSIONS:Findings confirmed that the two newly named species and the uncultivated phylotype targeted in this study are associated with different forms of apical periodontitis, and a pathogenetic or at least an ecologic role is suspected.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Rôças IN,Siqueira JF Jrdoi
10.1016/j.joen.2009.05.033subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1359-62issue
10eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00509-3journal_volume
35pub_type
杂志文章abstract：:This study assessed in vitro the diagnostic ability of cone beam computed tomography (CBCT) scans with different voxel resolutions in the detection of simulated external root resorption (ERR). For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.005
更新日期：2009-02-01 00:00:00
abstract：:It is thought that externally applied bleaching agents may penetrate into the pulp chamber. This study was conducted to evaluate the diffusion of peroxide bleaching agents into the pulp chamber of teeth restored with various restorative materials. Sixty-five human extracted anterior maxillary teeth were separated into...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200002000-00008
更新日期：2000-02-01 00:00:00
abstract：:This study evaluated the ability of ultrasonics and calcium hydroxide to remove pulp tissue debris from the mesial root canals of human mandibular molars. All teeth were instrumented using a standard filing technique and irrigated with an equal volume of 2.6% sodium hypochlorite before the application of the experimen...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(89)80076-7
更新日期：1989-08-01 00:00:00
abstract：:The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200007000-00001
更新日期：2000-07-01 00:00:00
abstract：:This case report describes the clinical, radiologic, and histopathologic features of the glandular odontogenic cyst (GOC). Although a relatively rare lesion not previously reported in the endodontic literature, the GOC is recommended for inclusion in a differential diagnosis of a dentoalveolar radiolucency. The GOC is...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00014
更新日期：2004-03-01 00:00:00
abstract：:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00004
更新日期：2004-03-01 00:00:00
abstract：INTRODUCTION:Instrumentation of the mesial root canal system of mandibular molars may hinder disinfection by packing hard tissue debris within the isthmuses. The removal of accumulated hard tissue debris (AHTD) by 3 supplemental irrigation systems, 2 ultrasonically activated and 1 multisonic, was assessed with micro-co...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.013
更新日期：2019-07-01 00:00:00
abstract：INTRODUCTION:This finite element analysis study was aimed at evaluating the effects of rotary systems on stresses in photopolymerized resin root models with oval-shaped canals. METHODS:Among the data collected by computed tomographic imaging, a mandibular second premolar tooth with an oval-shaped canal was selected, r...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.05.007
更新日期：2016-08-01 00:00:00
abstract：:Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80959-3
更新日期：1992-06-01 00:00:00
abstract：:The aim of this laboratory based study was to determine the shaping ability of Lightspeed nickel-titanium rotary instruments in simulated root canals. A total of 40 canals with four different shapes in terms of angle and position of curve were prepared with Lightspeed instruments using the stepback technique recommend...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80347-0
更新日期：1997-12-01 00:00:00
abstract：:Calcium hydroxide is the "gold standard" endodontic medication, but it may fail to eliminate certain facultative bacteria and yeasts found in root canal systems. In this study, standardized bovine dentin blocks infected with Enterococcus faecalis were treated with an aqueous calcium hydroxide or bioactive glass S53P4 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200404000-00009
更新日期：2004-04-01 00:00:00
abstract：INTRODUCTION:The treatment of immature necrotic permanent teeth presents several clinical challenges in endodontics. Regenerative endodontic procedures (REPs) permit root development, increased canal wall thickness, and apical closure. This longitudinal cohort study aimed to evaluate the long-term clinical and radiogra...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.035
更新日期：2017-03-01 00:00:00
abstract：:The mesiobuccal roots of 50 randomly selected maxillary first molars were examined to assess the incidence and position of the canal isthmus. Transverse serial sections of the apical 6 mm of each root were prepared in 1-mm increments. The apical side of each section was stained with methylene blue dye, viewed with a s...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80975-1
更新日期：1995-07-01 00:00:00
abstract：:In the rat, perforations of maxillary molars were created and treated with either tricalcium phosphate (Synthograft) or Cavit. At four time intervals (1 day, 1 wk, 2 wk, and 1 month), four evaluative factors (inflammation, bone resorption, cementum and dentin resorption, and epithelial proliferation) were analyzed and...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(89)80171-2
更新日期：1989-09-01 00:00:00
abstract：:In this retrospective study with limited cone-beam computed tomography (limited CBCT), we investigated the anatomic characteristics of the accessory mental foramina and accessory branches of the mandibular canal. The CBCT records of approximately 150 patients were evaluated, and 17 accessory mental foramina were found...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.033
更新日期：2008-12-01 00:00:00
abstract：INTRODUCTION:Dens invaginatus is a critical condition for endodontic treatment once it frequently presents a complex internal anatomy and might be associated with incomplete root and apical development. METHODS:Three cases of dens invaginatus with open apex in maxillary lateral incisors are presented. In the first cas...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2010.02.002
更新日期：2010-06-01 00:00:00
abstract：INTRODUCTION:The diagnosis and treatment planning of cracked teeth depend on the understanding of how cracks affect the surrounding tissues. This study evaluated the dentin and pulp conditions in teeth affected by cracks and attrition. METHODS:Specimens under investigation included 12 cracked posterior teeth and 8 tee...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.09.021
更新日期：2015-03-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to compare 2 reciprocating nickel-titanium systems with different manufacturing characteristics and to evaluate the influence of simulated clinical use on their torsional resistance. METHODS:New 25/.08 WaveOne Primary (WO; Dentsply Sirona, York, PA) and 25/.07 WaveOne Gold Primar...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.04.009
更新日期：2019-08-01 00:00:00
abstract：:Biopsy reports of specimens from the apices of 1659 teeth physically present at the time the biopsy was performed were reviewed. The following information was recorded from each report: (a) source of specimen, (b) sex of patient, (c) age of patient, (d) location of tooth associated with lesion, and (e) diagnoses repor...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/s0099-2399(06)81679-1
更新日期：1990-05-01 00:00:00
abstract：:Advanced glycation end products (AGEs) have been found to play a role in inflammation and vascular complications. The receptor for AGE (RAGE) is expressed on many cells and is up-regulated during inflammation. To date, RAGE expression or its correlation with nuclear factor kappa beta (NF-kappabeta) expression has not ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.02.013
更新日期：2008-06-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate porcine palatal mucosa dissolution by sodium hypochlorite (NaOCl) with or without an auxiliary dissolving agent containing glycocholic acid and a mixture of surfactants (Keratobacter [KB]; Saint Joseph DID, Valencia, Spain). METHODS:One hundred forty samples were obta...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.01.007
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:Many anatomical variations can occur within the mandibular first molar. Commonly, 3-4 canals are located, but as many as 6-7 canals have been reported. METHODS:This report describes a case of a mandibular first molar with 6 separate canals (3 mesial and 3 distal) that was instrumented with conventional ha...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.03.005
更新日期：2011-06-01 00:00:00
abstract：:Irreversibly inflamed pulp (IIP) constitutes both a pathophysiologic and a diagnostic challenge. Gingival crevicular fluid (GCF) samples were obtained with Periopaper strips from IIP and adjacent and contralateral teeth for interleukin-8 (CXCL8) and tumor necrosis factor-alpha (TNF-alpha) measurements. Pain intensity ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.10.022
更新日期：2008-02-01 00:00:00
abstract：:The purpose of this study was to investigate whether the inflammation of rat dental pulp induces the muscarinic acetylcholine receptor (mAChR) constitutive receptor activity. Pulpitis was induced with bacterial lipolysaccharide in rat incisors dental pulp. Saturation assay with [(3)H]-quinuclidinyl benzilate ([(3)H] Q...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.11.021
更新日期：2011-02-01 00:00:00
abstract：INTRODUCTION:The aim of the present study was to compare the immunoexpression of CD34, intercellular adhesion molecule-1 (ICAM-1), and podoplanin and the presence of mast cells with clinical, demographic, radiologic, and histologic features from periapical granulomas, periapical cysts, and residual cysts. METHODS:Thir...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.04.013
更新日期：2018-07-01 00:00:00
abstract：INTRODUCTION:Stem cells from the apical papilla (SCAPs) possess strong odonto/osteogenic differentiation potential. This study investigated the effect of cyclic adenosine monophosphate (cAMP) on odonto/osteogenic differentiation of SCAPs and the underlining interplay between cAMP and transforming growth factor beta 1 (...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.10.008
更新日期：2019-02-01 00:00:00
abstract：:The aim of this study was to evaluate the time required by four different root canal medications coupled with the temporary filling material Cavit (ESPE, Seefeld, Germany) to prevent penetration of bacteria into the root canal. There were 145 roots prepared in a standardized manner. Four groups with 15 samples each we...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.023
更新日期：2006-02-01 00:00:00
abstract：:Pulp regeneration is a biologic process occurring under specific circumstances. An endodontic treatment modality to accomplish pulp regeneration has emerged based on the response of undifferentiated cells that are often referred to as stem cells. The treatment itself is currently empirical based on clinicians' thought...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2014.01.017
更新日期：2014-04-01 00:00:00
abstract：INTRODUCTION:The occurrence of multiple injuries of the same tooth is extremely rare. This case report describes the treatment in a midroot and complicated crown-root-fractured permanent incisor that remained untreated for 4 years after the accident. METHODS:The tooth was treated by delayed partial pulpotomy using cal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.03.036
更新日期：2010-07-01 00:00:00
abstract：:This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a sta...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80521-2
更新日期：1995-10-01 00:00:00
