Abstract：
:Fibrosis-related events play a part in most blinding diseases worldwide. However, little is known about the mechanisms driving this complex multifactorial disease. Here we have carried out the first genome-wide RNA-Sequencing study in human conjunctival fibrosis. We isolated 10 primary fibrotic and 7 non-fibrotic conjunctival fibroblast cell lines from patients with and without previous glaucoma surgery, respectively. The patients were matched for ethnicity and age. We identified 246 genes that were differentially expressed by over two-fold and p < 0.05, of which 46 genes were upregulated and 200 genes were downregulated in the fibrotic cell lines compared to the non-fibrotic cell lines. We also carried out detailed gene ontology, KEGG, disease association, pathway commons, WikiPathways and protein network analyses, and identified distinct pathways linked to smooth muscle contraction, inflammatory cytokines, immune mediators, extracellular matrix proteins and oncogene expression. We further validated 11 genes that were highly upregulated or downregulated using real-time quantitative PCR and found a strong correlation between the RNA-Seq and qPCR results. Our study demonstrates that there is a distinct fibrosis gene signature in the conjunctiva after glaucoma surgery and provides new insights into the mechanistic pathways driving the complex fibrotic process in the eye and other tissues.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yu-Wai-Man C,Owen N,Lees J,Tagalakis AD,Hart SL,Webster AR,Orengo CA,Khaw PTdoi
10.1038/s41598-017-05780-5subject
Has Abstractpub_date
2017-07-17 00:00:00pages
5644issue
1issn
2045-2322pii
10.1038/s41598-017-05780-5journal_volume
7pub_type
杂志文章abstract：:Malaria surveillance data provide opportunity to develop forecasting models. Seasonal variability in environmental factors correlate with malaria transmission, thus the identification of transmission patterns is useful in developing prediction models. However, with changing seasonal transmission patterns, either due t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02560-z
更新日期：2017-06-01 00:00:00
abstract：:Pulsatile motion occurs in the cardiac systolic period and leads to significantly larger displacement of water molecules as it is observed during diffusion weighted image acquisition. Obvious pulsatile motion arises in the brain stem and basal ganglia and might affect the corticospinal tract. So far there is no consen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29525-0
更新日期：2018-07-25 00:00:00
abstract：:Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61699-4
更新日期：2020-03-16 00:00:00
abstract：:Smoking aggravates HIV-1 pathogenesis and leads to decreased responses to antiretroviral therapy. In this study, we aim to find a molecular mechanism that would explain smoking-induced HIV-1 replication. Benzo(a)pyrene (BaP), a major carcinogen in cigarette, requires metabolic activation through cytochrome P450s (CYPs...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28500-z
更新日期：2018-07-10 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-75176-5
更新日期：2020-10-28 00:00:00
abstract：:Checkpoint kinases are important in cellular surveillance pathways that help cells to cope with DNA damage and protect their genomes. In cycling cells, DNA replication is one of the most sensitive processes and therefore all organisms carefully regulate replication initiation and progression. The checkpoint kinase ATR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25238-6
更新日期：2018-05-02 00:00:00
abstract：:As an emerging sterilization technology, cold atmospheric plasma offers a dry, non-thermal, rapid process that is minimally damaging to a majority of substrates. However, the mechanisms by which plasma interacts with living cells are poorly understood and the plasma generation apparatuses are complex and resource-inte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35166-0
更新日期：2018-11-12 00:00:00
abstract：:The effective differentiation between multiple primary lung tumors (MPs) and intrapulmonary metastases (IMs) in patients is imperative to discover the exact disease stage and to select the most appropriate treatment. In this study, the authors was to evaluate the efficacy and validity of large-scale targeted sequencin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75935-4
更新日期：2020-11-02 00:00:00
abstract：:Diatoms are known for their intricate, silicified cell walls (frustules). Silica polymerization occurs in a compartment called the silica deposition vesicle (SDV) and it was proposed that the cytoskeleton influences silica patterning through the SDV membrane (silicalemma) via interactions with transmembrane proteins. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13613-8
更新日期：2017-10-18 00:00:00
abstract：:Surface enhanced Raman scattering (SERS) is generally and widely used to enhance the vibrational fingerprint of molecules located at the vicinity of noble metal nanoparticles. In this work, SERS is originally used to enhance the own vibrational density of states (VDOS) of nude and isolated gold nanoparticles. This off...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39164
更新日期：2016-12-16 00:00:00
abstract：:Brain diseases including neurological disorders and tumors remain under treated due to the challenge to access the brain, and blood-brain barrier (BBB) restricting drug delivery which, also profoundly limits the development of pharmacological treatment. Focused ultrasound (FUS) with microbubbles is the sole method to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25904-9
更新日期：2018-05-22 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-39693-2
更新日期：2019-04-17 00:00:00
abstract：:The recent outbreaks of the insect-vectored Zika virus have demonstrated its potential to be sexually transmitted, which complicates modeling and our understanding of disease dynamics. Autochthonous outbreaks in the US mainland may be a consequence of both modes of transmission, which affect the outbreak size, duratio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43651-3
更新日期：2019-05-10 00:00:00
abstract：:Although cooperation is central to the organisation of many social systems, relatively little is known about cooperation in situations of collective emergency. When groups of people flee from a danger such as a burning building or a terrorist attack, the collective benefit of cooperation is important, but the cost of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33417
更新日期：2016-09-15 00:00:00
abstract：:Increasingly, in vivo imaging holds a strategic position in bio-pharmaceutical innovation. We will present the implementation of an integrated multimodal imaging setup enabling the assessment of multiple, complementary parameters. The system allows the fusion of information provided by: Near infrared fluorescent bioma...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59561-8
更新日期：2020-02-14 00:00:00
abstract：:The functionalization of graphene remains an important challenge for numerous applications expected by this fascinating material. To keep advantageous properties of graphene after modification or functionalization of its structure, local approaches are a promising road. A novel technique is reported here that allows p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19719
更新日期：2016-01-29 00:00:00
abstract：:Peritrichously-flagellated bacteria, such as Escherichia coli, self-propel in fluids by using specialised motors to rotate multiple helical filaments. The rotation of each motor is transmitted to a short flexible segment called the hook which in turn transmits it to a flagellar filament, enabling swimming of the whole...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28319-8
更新日期：2018-07-16 00:00:00
abstract：:The aim is to design a patient-specific instrument (PSI) for multilevel cervical pedicle screw placement from C2 to C7, as well as verifying reliability and reproducibility. Computed tomography (CT) scans were obtained from 7 cadaveric cervical spines. Using Mimics software, semiautomatic segmentation was performed fo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51936-w
更新日期：2019-10-30 00:00:00
abstract：:To investigate the influence of various factors on the two outcome parameters "procedure - specific complication" (femoral head necrosis, infection, nonunion, femoral neck shortening, screw loosening, implant penetration) and "functional outcome" in patients with displaced and undisplaced femoral neck fracture treated...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57696-2
更新日期：2020-01-20 00:00:00
abstract：:There has been increasing interest in diatom-based bio-assessment but we still lack a comprehensive understanding of how to capture diatoms' temporal dynamics with an appropriate sampling frequency (ASF). To cover this research gap, we collected and analyzed daily riverine diatom samples over a 1-year period (25 April...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36950
更新日期：2016-11-14 00:00:00
abstract：:Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44317
更新日期：2017-03-20 00:00:00
abstract：:Chronic alcohol feeding causes lipid accumulation and apoptosis in the liver. This study investigated the role of bioactive lipid metabolites in alcohol-induced liver damage and tested the potential of targeting arachidonate 15-lipoxygenase (ALOX15) in treating alcoholic liver disease (ALD). Results showed that chroni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02759-0
更新日期：2017-08-21 00:00:00
abstract：:A series of β-NaLuF4 crystals were synthesized via a hydrothermal method. Hexagonal phase microdisks, microprisms, and microtubes were achieved by simply changing the amount of citric acid in the initial reaction solution. Pure red upconversion (UC) luminescence can be observed in β-NaLuF4:Yb(3+), Tm(3+), Er(3+) and L...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28051
更新日期：2016-06-16 00:00:00
abstract：:Although oxide-based resistive switching memory (OxRAM) is one of the strong next-generation high capacity memory candidates, it has the critical disadvantage that deviations of resistance levels is too severe to be adopted as a high capacity memory device. More specifically, it is known that the larger on/off current...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35838-x
更新日期：2019-01-22 00:00:00
abstract：:Dry eye disease (DED) is a highly prevalent, ocular disorder characterized by an abnormal tear film and ocular surface. Recent experimental data has suggested that the underlying pathology of DED involves inflammation of the lacrimal functional unit (LFU), comprising the cornea, conjunctiva, lacrimal gland and interco...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17869-y
更新日期：2017-12-13 00:00:00
abstract：:The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39118
更新日期：2016-12-20 00:00:00
abstract：:The pectin methylesterase action is usually studied in a homogeneous aqueous medium in the presence of a large excess of soluble substrate and water. However in the cell wall, the water content is much lower, the substrate is cross-linked with itself or with other polymers, and the enzyme has to diffuse through the so...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49108-x
更新日期：2019-08-29 00:00:00
abstract：:The complex architecture of the cortical part of the bovine-femur was examined to develop potential tissue engineering (TE) scaffolds. Weight-change and X-ray diffraction (XRD) results show that significant phase transformation and morphology conversion of the bone occur at 500-750°C and 750-900°C, respectively. Anoth...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05843
更新日期：2014-07-28 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-62766-6
更新日期：2020-04-06 00:00:00
abstract：:This paper analyzed the compositional and structural changes of humic acid (HA) after combined with phosphate fertilizer (PHA), and investigated its effects on the growth of maize seedlings with four humic acid concentrations. The results showed that the atomic ratios of O/C and (O + N)/N of PHA were significantly low...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74349-6
更新日期：2020-10-15 00:00:00
