Abstract:
:Checkpoint kinases are important in cellular surveillance pathways that help cells to cope with DNA damage and protect their genomes. In cycling cells, DNA replication is one of the most sensitive processes and therefore all organisms carefully regulate replication initiation and progression. The checkpoint kinase ATR plays important roles both in response to DNA damage and replication stress, and ATR inhibitors are currently in clinical trials for cancer treatment. Therefore, it is important to understand the roles of ATR in detail. Here we show that the fission yeast homologue Rad3 and the human ATR regulate events also in G1 phase in an unperturbed cell cycle. Rad3Δ mutants or human cells exposed to ATR inhibitor in G1 enter S phase prematurely, which results in increased DNA damage. Furthermore, ATR inhibition in a single G1 reduces clonogenic survival, demonstrating that long-term effects of ATR inhibition during G1 are deleterious for the cell. Interestingly, ATR inhibition through G1 and S phase reduces survival in an additive manner, strongly arguing that different functions of ATR are targeted in the different cell-cycle phases. We propose that potential effects of ATR inhibitors in G1 should be considered when designing future treatment protocols with such inhibitors.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bøe CA,Håland TW,Boye E,Syljuåsen RG,Grallert Bdoi
10.1038/s41598-018-25238-6subject
Has Abstractpub_date
2018-05-02 00:00:00pages
6880issue
1issn
2045-2322pii
10.1038/s41598-018-25238-6journal_volume
8pub_type
杂志文章abstract::The standard treatment for most acute myeloid leukemia (AML) is chemotherapy, which is often associated with severe adverse effects. One strategy to reduce the adverse effects is targeted therapy that can selectively deliver anticancer drugs to tumor cells. Immature laminin receptor protein (OFA/iLRP) is a potential t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43910-3
更新日期:2019-05-14 00:00:00
abstract::Klf5, a member of the Krüppel-like transcription factor family, has essential roles during embryonic development, cell proliferation, differentiation, migration and apoptosis. This study was to define molecular mechanism of Klf5 during the odontoblastic differentiation. The expression of Klf5, odontoblast-differentiat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46746
更新日期:2017-04-25 00:00:00
abstract::Microalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of no...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15980-8
更新日期:2017-11-20 00:00:00
abstract::Two cabbage (Brassica oleracea) cultivars 'Tekila' and 'Kilaherb' were identified as resistant to several pathotypes of Plasmodiophora brassicae. In this study, we identified a clubroot resistance gene (Rcr7) in 'Tekila' for resistance to pathotype 3 of P. brassicae from a segregating population derived from 'Tekila' ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36187-5
更新日期:2018-12-05 00:00:00
abstract::Human travel impacts the spread of infectious diseases across spatial and temporal scales, with broad implications for the biological and social sciences. Individual data on travel patterns have been difficult to obtain, particularly in low-income countries. Travel survey data provide detailed demographic information,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05678
更新日期:2014-07-14 00:00:00
abstract::This study aimed to identify and distinguish various factors that may influence the occurrence of mandibular coronoid fractures. From January 2000 to December 2009, a total of 1131 patients with maxillofacial fractures were enrolled in this statistical study to evaluate the association between mandibular coronoid frac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02335-6
更新日期:2017-05-19 00:00:00
abstract::Circularly polarized laser sources with small footprints and high efficiencies can possess advanced functionalities in optical communication and biophotonic integrated systems. However, the conventional lasers with additional circular-polarization converters are bulky and hardly compatible with nanophotonic circuits, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26578
更新日期:2016-05-25 00:00:00
abstract::Balancing the amounts of enzymes is one of the important factors to achieve optimum performance of a designed metabolic pathway. However, the random mutagenesis approach is impractical since it requires searching an unnecessarily large number of variants and often results in searching a narrow range of expression leve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04515
更新日期:2014-03-31 00:00:00
abstract::The anti-diabetic biguanide drugs metformin (METF) and phenformin (PHEN) may have anti-cancer effects. Biguanides suppress plasma growth factors, but nonetheless, the view that these mitochondrial inhibitors accumulate in tumor tissue to an extent that leads to severe energetic stress or alleviation of hypoxia-induced...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10010-z
更新日期:2017-08-25 00:00:00
abstract::Developmental changes in salamander skulls, before and after metamorphosis, affect the feeding capabilities of these animals. How changes in cranial morphology and tissue properties affect the function of the skull are key to decipher the early evolutionary history of the crown-group of salamanders. Here, 3D cranial b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10553-1
更新日期:2017-08-31 00:00:00
abstract::Thermal rectifiers whose forward heat fluxes are greater than reverse counterparts have been extensively studied. Here we have discovered, idealized, and derived the ultimate limit of such rectification ratios, which are partially validated by numerical simulations, experiments, and micro-scale Hamiltonian-oscillator ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12677
更新日期:2015-08-04 00:00:00
abstract::Upconversion nanoparticles (UCNPs) are utilized extensively for biomedical imaging, sensing, and therapeutic applications, yet the molecular weight of UCNPs has not previously been reported. Herein, we present a theory based upon the crystal structure of UCNPs to estimate the molecular weight of UCNPs: enabling insigh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19415-w
更新日期:2018-01-18 00:00:00
abstract::Are musicians better able to understand speech in noise than non-musicians? Recent findings have produced contradictory results. Here we addressed this question by asking musicians and non-musicians to understand target sentences masked by other sentences presented from different spatial locations, the classical 'cock...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11628
更新日期:2015-06-26 00:00:00
abstract::Preterm infants are susceptible to inflammation-induced white matter injury but the exposures that lead to this are uncertain. Histologic chorioamnionitis (HCA) reflects intrauterine inflammation, can trigger a fetal inflammatory response, and is closely associated with premature birth. In a cohort of 90 preterm infan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37932
更新日期:2016-12-01 00:00:00
abstract::A plasmonic Ag/TiO2 photocatalytic composite was designed by selecting Ag quantum dots (Ag QDs) to act as a surface plasmon resonance (SPR) photosensitizer for driving the visible-light driven photoelectrocatalytic hydrogen evolution. Vertically oriented hierarchical TiO2 nanotube arrays (H-TiO2-NTAs) with macroporous...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10461
更新日期:2015-06-12 00:00:00
abstract::Positions of leaves along a stem usually adhere to a genetically determined, species-specific pattern known as a leaf phyllotaxis. We investigated whether the arrangement of lateral secondary veins along primary midveins adhered to a species-specific pattern that resembled an alternate or opposite phyllotaxis. We anal...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34772-2
更新日期:2018-11-06 00:00:00
abstract::Since the advent of graphene, a variety of studies have been performed to elucidate its fundamental physics, or to explore its practical applications. Gate-tunable resistance is one of the most important properties of graphene and has been studied in 1-3 layer graphene in a number of efforts to control the band gap to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32214-7
更新日期:2018-09-18 00:00:00
abstract::The discovery of Weyl semimetals (WSM) has brought forth the condensed matter realization of Weyl fermions, which were previously theorized as low energy excitations in high energy particle physics. Recently, transition metal mono-pnictides are under intense investigation for understanding properties of inversion-symm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46062
更新日期:2017-04-10 00:00:00
abstract::Trimethylation on H3K27 mediated by Polycomb Repressive Complex 2 (PRC2) is required to control gene repression programs involved in development, regulation of tissue homeostasis or maintenance and lineage specification of stem cells. In Drosophila, the PRC2 catalytic subunit is the single protein E(z), while in mamma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40738-9
更新日期:2019-03-13 00:00:00
abstract::Systemic lupus erythematosus (SLE) flares elicit progressive organ damage, leading to disability and early mortality. This study evaluated clinical and immunologic factors associated with impending flare in the Biomarkers of Lupus Disease study. Autoantibodies and 32 soluble mediators were measured by multiplex assays...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45135-w
更新日期:2019-06-13 00:00:00
abstract::The prominent comprehensive properties of solid-solution- and intermetallic-based Ti alloys are derived from their diverse microstructures induced by multi-component alloying, which results in a chemical composition complexity. A cluster-plus-glue-atom model, characterizing the chemical short-range orders, was introdu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40302-5
更新日期:2019-03-04 00:00:00
abstract::Responses of ecosystems to human-induced stress vary in space and time, because both stressors and ecosystem vulnerabilities vary in space and time. Presently, ecosystem impact assessments mainly take into account variation in stressors, without considering variation in ecosystem vulnerability. We developed a method t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09573-8
更新日期:2017-08-24 00:00:00
abstract::Streptococcus pneumoniae (S. pneumoniae) is a leading human pathogen, which takes large responsibility for severe otitis media, acute meningitis and septicaemia. It encodes up to three distinct sialidases: NanA, NanB and NanC, which are promising drug targets. Recent experimental studies have shown that these three si...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38131-z
更新日期:2019-02-15 00:00:00
abstract::Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-ter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09033-3
更新日期:2017-08-17 00:00:00
abstract::Kohlschutter-Tönz syndrome (KTS) is a rare genetic disorder with neurological dysfunctions including seizure and intellectual impairment. Mutations at the Rogdi locus have been linked to development of KTS, yet the underlying mechanisms remain elusive. Here we demonstrate that a Drosophila homolog of Rogdi acts as a n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11941-3
更新日期:2017-09-12 00:00:00
abstract::LiYF4:Eu nanophosphors with a single tetragonal phase are synthesized, and various strategies to enhance the Eu(3+) emission from the nanophosphors are investigated. The optimized Eu(3+) concentration is 35 mol%, and the red emission peaks due to the (5)D0 →(7)FJ (J = 1 and 2) transitions of Eu(3+) ions are further en...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07866
更新日期:2015-01-19 00:00:00
abstract::Most gliomas are invasive tumors formed from glial cells and associated with high mortality rates. In this study, we characterized four glioma cell lines of varying degree of aggressiveness (H4, SW1088, A172 and U87-MG) in terms of morphology, cytoskeleton organization and stiffness, and evaluated their invasive poten...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44200-8
更新日期:2019-05-23 00:00:00
abstract::The overproduction of hydrogen peroxide is an inherent feature of some tumour cells and inflamed tissues. We took advantage of this peculiarity to eliminate cells using chemiluminescent peroxyoxalate reaction. We designed dispersions containing polyoxalate and tetramethylhematoporhyrin (TMHP) in dimethylphthalate drop...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03527-w
更新日期:2017-06-13 00:00:00
abstract::Histone methylation is thought to control the regulation of genetic program and the dysregulation of it has been found to be closely associated with cancer. JMJD3 has been identified as an H3K27 demethylase and its role in cancer development is context specific. The role of JMJD3 in gastric cancer (GC) has not been ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37340-w
更新日期:2019-01-29 00:00:00
abstract::Delta-9-tetrahydrocannabinol (THC) is the primary psychoactive compound in Cannabis, which is studied extensively for its medicinal value. A central gap in the science is the underlying mechanisms surrounding THC's therapeutic effects and the role of gut metabolite profiles. Using a mass-spectrometry based metabolomic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46478-0
更新日期:2019-07-19 00:00:00