Abstract：
:Balancing the amounts of enzymes is one of the important factors to achieve optimum performance of a designed metabolic pathway. However, the random mutagenesis approach is impractical since it requires searching an unnecessarily large number of variants and often results in searching a narrow range of expression levels which are out of optimal level. Here, we developed a predictive combinatorial design method, called UTR Library Designer, which systematically searches a large combinatorial space of expression levels. It accomplishes this by designing synthetic translation initiation region of mRNAs in a predictive way based on a thermodynamic model and genetic algorithm. Using this approach, we successfully enhanced lysine and hydrogen production in Escherichia coli. Our method significantly reduced the number of variants to be explored for covering large combinatorial space and efficiently enhanced pathway efficiency, thereby facilitating future efforts in metabolic engineering and synthetic biology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Seo SW,Yang JS,Cho HS,Yang J,Kim SC,Park JM,Kim S,Jung GYdoi
10.1038/srep04515subject
Has Abstractpub_date
2014-03-31 00:00:00pages
4515issn
2045-2322pii
srep04515journal_volume
4pub_type
杂志文章abstract：:Hbs1, which is homologous to the GTPase eRF3, is a small G protein implicated in mRNA quality control. It interacts with a translation-release factor 1-like protein Dom34/Pelota to direct decay of mRNAs with ribosomal stalls. Although both proteins are evolutionarily conserved in eukaryotes, the biological function of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39530-6
更新日期：2019-03-01 00:00:00
abstract：:Modes attenuation of the tube lattice fiber (TLF) is characterized by D/λ, where D is the core diameter and λ is the wavelength. Hence, the TLF is structured with a large core to ensure a low attenuation loss. A small core, on the other hand, facilitates the gas-filled TLF applications, but at the expense of the incre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01839-5
更新日期：2017-05-09 00:00:00
abstract：:Layered-structure materials are currently relevant given their quasi-2D nature. Knowledge of their physical properties is currently of major interest. Niobium ditelluride possesses a monoclinic layered-structure with a distortion in the tellurium planes. This structural complexity has hindered the determination of its...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35308-4
更新日期：2018-11-19 00:00:00
abstract：:Although the growth of graphene by chemical vapor deposition is a production technique that guarantees high crystallinity and superior electronic properties on large areas, it is still a challenge for manufacturers to efficiently scale up the production to the industrial scale. In this context, issues related to the p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09811-z
更新日期：2017-08-30 00:00:00
abstract：:Polystyrene sulfonate (PSS), a strong polyelectrolyte, was used to prepare red photoluminescent PSS-penicillamine (PA) copper (Cu) nanoclusters (NC) aggregates, which displayed high selectivity and sensitivity to the detection of hydrogen sulfide (H2S). The size of the PSS-PA-Cu NC aggregates could be readily controll...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24882
更新日期：2016-04-26 00:00:00
abstract：:Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02001-x
更新日期：2017-05-15 00:00:00
abstract：:Carbonatites, usually occurring within intra-continental rift-related settings, have strong light rare earth element (LREE) enrichment; they rarely contain economic heavy REE (HREE). Here, we report the identification of Late Triassic HREE-Mo-rich carbonatites in the northernmost Qinling orogen. The rocks contain abun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37377
更新日期：2016-11-18 00:00:00
abstract：:Generalized Auto-calibrating Partially Parallel Acquisitions (GRAPPA) has been widely used to reduce imaging time in Magnetic Resonance Imaging. GRAPPA synthesizes missing data by using a linear interpolation of neighboring measured data over all coils, thus accuracy of the interpolation weights fitting to the auto-ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33171-x
更新日期：2018-10-10 00:00:00
abstract：:There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24236-y
更新日期：2018-05-08 00:00:00
abstract：:Enamel proteins form a scaffold for growing hydroxyapatite crystals during enamel formation. They are then almost completely degraded during enamel maturation, resulting in a protein content of only 1% (w/v) in mature enamel. Nevertheless, this small amount of remaining proteins has important effects on the mechanical...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15194
更新日期：2015-10-15 00:00:00
abstract：:As an emerging sterilization technology, cold atmospheric plasma offers a dry, non-thermal, rapid process that is minimally damaging to a majority of substrates. However, the mechanisms by which plasma interacts with living cells are poorly understood and the plasma generation apparatuses are complex and resource-inte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35166-0
更新日期：2018-11-12 00:00:00
abstract：:The immune responses of males and females to bacterial infections display differences. The mechanisms that underlie this sexual dimorphism are multifactorial. Lipopolysaccharide (LPS) contributes to the pathogenesis of endotoxaemia. We have previously demonstrated that the plasma protein beta-2 glycoprotein-1 (β2GPI) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07945-8
更新日期：2017-08-15 00:00:00
abstract：:This was a nine-year retrospective cohort study to investigate obstetric and perinatal outcomes in a cohort of adolescent girls with twin pregnancies from a major Australian tertiary centre in Brisbane, Australia. The adolescent cohort was aged <19 years and the control group was aged 20-24 years. The total study coho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37364-2
更新日期：2018-12-24 00:00:00
abstract：:Selective removal of lignin and other recalcitrant compounds, collectively registered as acid-unhyrolyzable residue (AUR), results in bleaching of leaf litter, but the importance of bleaching in decomposition processes on forest soil has not been fully evaluated. The aims of this study were to elucidate the occurrence...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81206-7
更新日期：2021-01-19 00:00:00
abstract：:Impaired wound healing is an immense medical challenge, and while autologous skin grafting remains the "gold-standard" therapeutic option for repairing wounds that cannot be closed by primary or secondary intention, it is limited by substantial donor site morbidity. We previously developed the alternative approach of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81179-7
更新日期：2021-01-18 00:00:00
abstract：:Worldwide biodiversity assessments have mainly focused on species richness but little is known about the diversity of species roles, i.e. functional diversity, while this is a key facet to understanding the consequences of global changes on the ecosystem services to human societies. Here, we report the world pattern o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22125
更新日期：2016-03-16 00:00:00
abstract：:Hologram memory is expected to be the next-generation of optical data storage technology. Bismuth-substituted yttrium iron garnet is typically used for rewritable magnetic hologram media. The diffraction efficiency of magnetic holography depends on the Faraday rotation angle, but the experimental diffraction efficienc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12442-z
更新日期：2017-10-09 00:00:00
abstract：:Previous studies highlight the winners and losers in fisheries under climate change based on shifts in biomass, species composition and potential catches. Understanding how climate change is likely to alter the fisheries revenues of maritime countries is a crucial next step towards the development of effective socio-e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32607
更新日期：2016-09-07 00:00:00
abstract：:Extracellular vesicles (EVs) are an important class of membrane-bound structures that have been widely investigated for their roles in intercellular communication in the contexts of tumor progression, vascular function, immunity and regenerative medicine. Much of the current knowledge on the functions of EVs pertains ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44021-9
更新日期：2019-05-17 00:00:00
abstract：:Plutonium isotopes have primarily been injected to the stratosphere by the atmospheric nuclear weapon tests and the burn-up of the SNAP-9A satellite. Here we show by using published data that the stratospheric plutonium exponentially decreased with apparent residence time of 1.5 ± 0.5 years, and that the temporal vari...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15707
更新日期：2015-10-28 00:00:00
abstract：:Adverse maternal environments, such as diabetes and obesity, impair placental mitochondrial function, which affects fetal development and offspring long-term health. The underlying mechanisms and effective interventions to abrogate such effect remain unclear. Our previous studies demonstrated impaired mitochondrial bi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65415-0
更新日期：2020-05-20 00:00:00
abstract：:Central visual field (CVF) loss has been linked to poorer vision-related quality of life in eyes with open-angle glaucoma (OAG) and can occur even in early-stage OAG eyes. The present study investigated whether the detection of choroidal microvasculature dropout (CMvD) during follow-up, which may be a sign of perfusio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44942-5
更新日期：2019-06-12 00:00:00
abstract：:INK4 locus at chromosome 9p21 has been reported to be associated with primary open angle glaucoma (POAG) and its subtypes along with the associated optic disc parameters across the populations of European, Japanese and African ancestries. The locus encodes three tumor suppressor genes namely CDKN2A, ARF, CDKN2B and a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05115
更新日期：2014-05-30 00:00:00
abstract：:The discovery of the community structure of real-world networks is still an open problem. Many methods have been proposed to shed light on this problem, and most of these have focused on discovering node community. However, link community is also a powerful framework for discovering overlapping communities. Here we pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22470
更新日期：2016-03-01 00:00:00
abstract：:Neural crest cells (NCC) are stem cells that generate different lineages, including neuroendocrine, melanocytic, cartilage, and bone. The differentiation potential of NCC varies according to the level from which cells emerge along the neural tube. For example, only anterior "cranial" NCC form craniofacial bone, wherea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19727
更新日期：2016-01-27 00:00:00
abstract：:Crimean-Congo hemorrhagic fever virus (CCHFV), a tick-borne bunyavirus, can cause a life-threatening hemorrhagic syndrome in humans but not in its animal host. The virus is widely distributed throughout southeastern Europe, the Middle East, Africa, and Asia. Disease management has proven difficult and there are no bro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44210-6
更新日期：2019-05-23 00:00:00
abstract：:Genetic alterations identified in adjacent normal appearing tissue in bladder cancer patients are indicative of a field disease. Here we assessed normal urothelium transformation and intra-tumour heterogeneity (ITH) in four patients with bladder cancer. Exome sequencing identified private acquired mutations in a lymph...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11291-0
更新日期：2017-09-15 00:00:00
abstract：:Skin burns are a significant source of injury in both military and civilian sectors. They are especially problematic in low resource environments where non-fatal injuries can lead to high morbidity rates, prolonged hospitalization, and disability. These multifaceted wounds can be highly complex and must be quickly dia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65747-x
更新日期：2020-06-09 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related mortality worldwide. It is urgently needed to develop potential drugs with good efficacy and low toxicity for HCC treatment. The anti-tumor effect of Traditional Chinese Medicine (TCM) has received increasing attention worldwide. Tram...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31298
更新日期：2016-08-09 00:00:00
abstract：:Solvent-free, nonvolatile, room-temperature alkylated-π functional molecular liquids (FMLs) are rapidly emerging as a new generation of fluid matter. However, precision design to tune their physicochemical properties remains a serious challenge because the properties are governed by subtle π-π interactions among funct...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03584-1
更新日期：2017-06-13 00:00:00
