DC-SIGN and Toll-like receptor 4 mediate oxidized low-density lipoprotein-induced inflammatory responses in macrophages.

Abstract:

:The regulation of inflammatory responses by innate immune receptors is recognized as a crucial step in the development of atherosclerosis, although the precise molecular mechanisms remain to be elucidated. This study focused on illustrating the roles of dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN)- and Toll-like receptor 4 (TLR4)-regulated inflammatory responses in macrophages. We found that DC-SIGN expression levels were increased in macrophages of atherosclerotic plaques. Oxidized low-density lipoprotein (oxLDL) significantly enhanced DC-SIGN protein expression levels after a short-term exposure. Knockdown of DC-SIGN decreased expression and secretion of interleukin 1-β (IL1-β), monocyte chemo-attractant protein 1 (MCP-1), tumor necrosis factor-α (TNFα) and matrix metalloproteinase-9 (MMP-9). Immunofluorescence studies demonstrated that DC-SIGN and TLR4 co-localized in regions of the plaques. Moreover, DC-SIGN was co-expressed with TLR4 on the plasma membrane after oxLDL stimulation. The presence of an endogenous interaction and the results of the in vitro pull-down assays revealed that DC-SIGN binds directly with TLR4. We also present evidence that DC-SIGN mediates TLR4-regulated NFκB activation but not activation of p38 and JNK. Our results suggest an essential role of DC-SIGN/TLR4 signaling in macrophages in the pathogenesis of atherosclerosis.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Yang K,Liu X,Liu Y,Wang X,Cao L,Zhang X,Xu C,Shen W,Zhou T

doi

10.1038/s41598-017-03740-7

subject

Has Abstract

pub_date

2017-06-12 00:00:00

pages

3296

issue

1

issn

2045-2322

pii

10.1038/s41598-017-03740-7

journal_volume

7

pub_type

杂志文章
  • Evolution and Determinants of Lung Function until Late Infancy among Infants Born Preterm.

    abstract::To investigate the evolution of lung function in preterm infants with and without bronchopulmonary dysplasia (BPD) and to determine the perinatal characteristics associated with indexes of lung function in later infancy. Longitudinal lung function assessments were performed at approximately 6, 12, 18, and 24 months of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57359-x

    authors: Lai SH,Chiang MC,Chu SM,Hsu JF,Yao TC,Tsai MH,Hua MC,Chiu CY,Yeh KW,Huang JL,Liao SL

    更新日期:2020-01-16 00:00:00

  • The microbiomes of blowflies and houseflies as bacterial transmission reservoirs.

    abstract::Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16353-x

    authors: Junqueira ACM,Ratan A,Acerbi E,Drautz-Moses DI,Premkrishnan BNV,Costea PI,Linz B,Purbojati RW,Paulo DF,Gaultier NE,Subramanian P,Hasan NA,Colwell RR,Bork P,Azeredo-Espin AML,Bryant DA,Schuster SC

    更新日期:2017-11-24 00:00:00

  • Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1-/- phenotype and forms complexes with wildtype PS1 and nicastrin.

    abstract::The presenilin 1 (PSEN1) L271V mutation causes early-onset familial Alzheimer's disease by disrupting the alternative splicing of the PSEN1 gene, producing some transcripts harboring the L271V point mutation and other transcripts lacking exon 8 (PS1(∆exon8)). We previously reported that PS1 L271V increased amyloid bet...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17042

    authors: Brautigam H,Moreno CL,Steele JW,Bogush A,Dickstein DL,Kwok JB,Schofield PR,Thinakaran G,Mathews PM,Hof PR,Gandy S,Ehrlich ME

    更新日期:2015-11-26 00:00:00

  • The ubiquitin ligase Cullin5SOCS2 regulates NDR1/STK38 stability and NF-κB transactivation.

    abstract::SOCS2 is a pleiotropic E3 ligase. Its deficiency is associated with gigantism and organismal lethality upon inflammatory challenge. However, mechanistic understanding of SOCS2 function is dismal due to our unawareness of its protein substrates. We performed a mass spectrometry based proteomic profiling upon SOCS2 depl...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42800

    authors: Paul I,Batth TS,Iglesias-Gato D,Al-Araimi A,Al-Haddabi I,Alkharusi A,Norstedt G,Olsen JV,Zadjali F,Flores-Morales A

    更新日期:2017-02-20 00:00:00

  • Author Correction: Novel Indole-fused benzo-oxazepines (IFBOs) inhibit invasion of hepatocellular carcinoma by targeting IL-6 mediated JAK2/STAT3 oncogenic signals.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-59134-9

    authors: Singh AK,Bhadauria AS,Kumar U,Raj V,Rai A,Kumar P,Keshari AK,Kumar D,Maity B,Nath S,Prakash A,Saha S

    更新日期:2020-02-06 00:00:00

  • 2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione inhibits the growth and metastasis of breast carcinoma in mice.

    abstract::Metastasis causes approximately 90% of breast cancer-related deaths in women. Previously, we have demonstrated that 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione (DMDD) remarkably inhibited the growth of human breast cancer cells with little toxicity. In this study, we investigated the toxicity and efficacy of DMD...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07162-3

    authors: Chen C,Nong Z,Xie Q,He J,Cai W,Tang X,Chen X,Huang R,Gao Y

    更新日期:2017-07-27 00:00:00

  • Conductivity-limiting bipolar thermal conductivity in semiconductors.

    abstract::Intriguing experimental results raised the question about the fundamental mechanisms governing the electron-hole coupling induced bipolar thermal conduction in semiconductors. Our combined theoretical analysis and experimental measurements show that in semiconductors bipolar thermal transport is in general a "conducti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10136

    authors: Wang S,Yang J,Toll T,Yang J,Zhang W,Tang X

    更新日期:2015-05-13 00:00:00

  • Supramolecular polymerization of a prebiotic nucleoside provides insights into the creation of sequence-controlled polymers.

    abstract::Self-assembly of a nucleoside on Au(111) was studied to ascertain whether polymerization on well-defined substrates constitutes a promising approach for making sequence-controlled polymers. Scanning tunneling microscopy and density functional theory were used to investigate the self-assembly on Au(111) of (RS)-N(9)-(2...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep18891

    authors: Wang J,Bonnesen PV,Rangel E,Vallejo E,Sanchez-Castillo A,James Cleaves Ii H,Baddorf AP,Sumpter BG,Pan M,Maksymovych P,Fuentes-Cabrera M

    更新日期:2016-01-04 00:00:00

  • Increased temperatures and elevated CO2 levels reduce the sensitivity of Conyza canadensis and Chenopodium album to glyphosate.

    abstract::Herbicides are the most commonly used means of controlling weeds. Recently, there has been growing concern over the potential impacts of global climate change, specifically, increasing temperatures and elevated carbon dioxide (CO2) concentrations, on the sensitivity of weeds to herbicides. Here, glyphosate response of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-38729-x

    authors: Matzrafi M,Brunharo C,Tehranchian P,Hanson BD,Jasieniuk M

    更新日期:2019-02-18 00:00:00

  • Biogenic synthesis of AgNPs employing Terminalia arjuna leaf extract and its efficacy towards catalytic degradation of organic dyes.

    abstract::In the present work, we demonstrated the biosynthesis of silver nanoparticles (AgNPs) by highly stable, economic and eco-friendly method using leaf extract of Terminalia arjuna (T. arjuna) and employing as a catalyst for the degradation of methyl orange (MO), methylene blue (MB), congo red (CR) and 4- nitrophenol (4-N...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-66851-8

    authors: Raj S,Singh H,Trivedi R,Soni V

    更新日期:2020-06-15 00:00:00

  • A new mass spectrometry-based method for the quantification of histones in plasma from septic shock patients.

    abstract::The aim of this study was to develop a novel method to detect circulating histones H3 and H2B in plasma based on multiple reaction monitoring targeted mass spectrometry and a multiple reaction monitoring approach (MRM-MS) for its clinical application in critical bacteriaemic septic shock patients. Plasma samples from ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10830-z

    authors: García-Giménez JL,Romá-Mateo C,Carbonell N,Palacios L,Peiró-Chova L,García-López E,García-Simón M,Lahuerta R,Gimenez-Garzó C,Berenguer-Pascual E,Mora MI,Valero ML,Alpízar A,Corrales FJ,Blanquer J,Pallardó FV

    更新日期:2017-09-06 00:00:00

  • Extending the Spectrum of Dysgraphia: A Data Driven Strategy to Estimate Handwriting Quality.

    abstract::This paper proposes new ways to assess handwriting, a critical skill in any child's school journey. Traditionally, a pen and paper test called the BHK test (Concise Evaluation Scale for Children's Handwriting) is used to assess children's handwriting in French-speaking countries. Any child with a BHK score above a cer...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60011-8

    authors: Asselborn T,Chapatte M,Dillenbourg P

    更新日期:2020-02-21 00:00:00

  • LC-HRMS based approach to identify novel sphingolipid biomarkers in breast cancer patients.

    abstract::Perturbations in lipid metabolic pathways to meet the bioenergetic and biosynthetic requirements is a principal characteristic of cancer cells. Sphingolipids (SPLs) are the largest class of bioactive lipids associated to various aspects of tumorigenesis and have been extensively studied in cancer cell lines and experi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61283-w

    authors: Bhadwal P,Dahiya D,Shinde D,Vaiphei K,Math RGH,Randhawa V,Agnihotri N

    更新日期:2020-03-13 00:00:00

  • Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease.

    abstract::Neurodegeneration includes acute changes and slow-developing alterations, both of which partly involve common cellular machinery. During neurodegeneration, neuronal processes are impaired along with dysregulated post-translational modifications (PTMs) of cytoskeletal proteins. In neuronal processes, tubulin undergoes ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40205

    authors: Vu HT,Akatsu H,Hashizume Y,Setou M,Ikegami K

    更新日期:2017-01-09 00:00:00

  • Study of Protein Expression in Peri-Infarct Tissue after Cerebral Ischemia.

    abstract::In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12030

    authors: Brea D,Agulla J,Staes A,Gevaert K,Campos F,Sobrino T,Blanco M,Dávalos A,Castillo J,Ramos-Cabrer P

    更新日期:2015-07-08 00:00:00

  • Minocycline, a microglial inhibitor, reduces 'honey trap' risk in human economic exchange.

    abstract::Recently, minocycline, a tetracycline antibiotic, has been reported to improve symptoms of psychiatric disorders and to facilitate sober decision-making in healthy human subjects. Here we show that minocycline also reduces the risk of the 'honey trap' during an economic exchange. Males tend to cooperate with physicall...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01685

    authors: Watabe M,Kato TA,Tsuboi S,Ishikawa K,Hashiya K,Monji A,Utsumi H,Kanba S

    更新日期:2013-01-01 00:00:00

  • North Atlantic controls on wintertime warm extremes and aridification trends in the Middle East.

    abstract::The Middle East is one of the most water stressed regions in the world, receiving the majority of its hydrological input during the winter, in the form of highly variable and scattered precipitation. The persistence of wintertime anticyclonic conditions over the region can deflect storm tracks and result in extended s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12430-3

    authors: Kumar KN,Molini A,Ouarda TBMJ,Rajeevan MN

    更新日期:2017-09-26 00:00:00

  • Neuronal imaging with ultrahigh dynamic range multiphoton microscopy.

    abstract::Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06065-7

    authors: Yang R,Weber TD,Witkowski ED,Davison IG,Mertz J

    更新日期:2017-07-19 00:00:00

  • Optokinetic stimulation induces vertical vergence, possibly through a non-visual pathway.

    abstract::Vertical vergence is generally associated with one of three mechanisms: vestibular activation during a head tilt, induced by vertical visual disparity, or as a by-product of ocular torsion. However, vertical vergence can also be induced by seemingly unrelated visual conditions, such as optokinetic rotations. This stud...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72646-8

    authors: Wibble T,Pansell T

    更新日期:2020-09-23 00:00:00

  • Preparation of cross-linked hen-egg white lysozyme crystals free of cracks.

    abstract::Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the crystals, which may fi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34770

    authors: Yan EK,Lu QQ,Zhang CY,Liu YL,He J,Chen D,Wang B,Zhou RB,Wu P,Yin DC

    更新日期:2016-10-05 00:00:00

  • A Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markers.

    abstract::Accurate cancer diagnosis often requires extraction and purification of genetic materials from cells, and sophisticated instrumentations that follow. Otherwise in order to directly treat the diagnostic materials to cells, multiple steps to optimize dose concentration and treatment time are necessary due to diversity i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep18497

    authors: Park KS,Shin SW,Jang MS,Shin W,Yang K,Min J,Cho SW,Oh BK,Bae JW,Jung S,Choi JW,Um SH

    更新日期:2015-12-18 00:00:00

  • Superdirective dimers of coupled self-resonant split ring resonators: Analytical modelling and numerical and experimental validation.

    abstract::Superdirective antennas developed over the last century have received renewed interest in recent years from the development of metamaterials. These arrays of electromagnetic resonators (or meta-atoms) carrying short wavelength electro- and/or magneto-inductive waves support current distributions with very high spatial...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56988-6

    authors: Vallecchi A,Radkovskaya A,Li L,Faulkner G,Stevens CJ,Shamonina E

    更新日期:2020-01-14 00:00:00

  • Trivalent Y3+ ionic sensor development based on (E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) derivatives modified with nafion matrix.

    abstract::(E)-Methyl-N'-nitrobenzylidene-benzenesulfonohydrazide (MNBBSH) compounds were synthesized using a condensation procedure from the derivatives of nitrobenzaldehyde and 4-Methyl-benzenesulfonylhydrazine, which crystallized in ethanol and methanol as well as characterized by FTIR, UV-Vis, 1H-NMR, and 13C-NMR. MNBBSH str...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05703-4

    authors: Hussain MM,Rahman MM,Arshad MN,Asiri AM

    更新日期:2017-07-19 00:00:00

  • Effect of acoustic standing waves on cellular viability and metabolic activity.

    abstract::Acoustic standing wave devices offer excellent potential applications in biological sciences for drug delivery, cell manipulation and tissue engineering. However, concerns have been raised about possible destructive effects on cells due to the applied acoustic field, in addition to other produced secondary factors. He...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-65241-4

    authors: Levario-Diaz V,Bhaskar P,Carmen Galan M,Barnes AC

    更新日期:2020-05-22 00:00:00

  • A mask R-CNN model for reidentifying extratropical cyclones based on quasi-supervised thought.

    abstract::The applications of machine learning/deep learning (ML/DL) methods in meteorology have developed considerably in recent years. Massive amounts of meteorological data are conducive to improving the training effect and model performance of ML/DL, but the establishment of training datasets is often time consuming, especi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71831-z

    authors: Lu C,Kong Y,Guan Z

    更新日期:2020-09-14 00:00:00

  • RNA sequencing reveals a transcriptomic portrait of human mesenchymal stem cells from bone marrow, adipose tissue, and palatine tonsils.

    abstract::Human mesenchymal stem cells (MSCs) are adult multipotent cells that have plasticity and inhabit the stroma of diverse tissues. The potential utility of MSCs has been heavily investigated in the fields of regenerative medicine and cell therapy. However, MSCs represent diverse populations that may depend on the tissue ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16788-2

    authors: Cho KA,Park M,Kim YH,Woo SY,Ryu KH

    更新日期:2017-12-07 00:00:00

  • (-)-Gochnatiolide B, synthesized from dehydrocostuslactone, exhibits potent anti-bladder cancer activity in vitro and in vivo.

    abstract::With limited success achieved in bladder cancer patient management, novel agents are in urgent need for the purpose of therapy and prevention. As a sesquiterpenoid dimmer isolated from Gochnatia pomculat, (-)-gochnatiolide B has been bio-mimetically synthesized in multiple steps with a poor yield, which heavily limite...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27036-6

    authors: Chen Y,Li W,Zeng Z,Tang Y

    更新日期:2018-06-11 00:00:00

  • Shape-modification of patterned nanoparticles by an ion beam treatment.

    abstract::This paper evaluated a practical approach to the fabrication of arrays of non-spherical nanoparticles by colloidal etching without a mask involving exposure to a low energy ion beam. A spherical nanoparticle array was transferred using a soft nanolithography technique, which is a simple and effective pattern transfer ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08523

    authors: Heo KC,Gwag JS

    更新日期:2015-02-17 00:00:00

  • Tolerance and responsive gene expression of Sogatella furcifera under extreme temperature stresses are altered by its vectored plant virus.

    abstract::Southern rice black-streaked dwarf virus (SRBSDV), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern Asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (WBPH, Sogatella furcifera) in a persistent, circulative propagative manner...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31521

    authors: Xu D,Zhong T,Feng W,Zhou G

    更新日期:2016-08-17 00:00:00

  • Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus.

    abstract::Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01432-w

    authors: Zang L,Shimada Y,Nishimura N

    更新日期:2017-05-03 00:00:00