Study of Protein Expression in Peri-Infarct Tissue after Cerebral Ischemia.

Abstract:

:In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contra-lateral tissues (at least 3-fold, p < 0.05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70 kDa family of heat shock proteins were highly expressed in the peri-infarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Brea D,Agulla J,Staes A,Gevaert K,Campos F,Sobrino T,Blanco M,Dávalos A,Castillo J,Ramos-Cabrer P

doi

10.1038/srep12030

subject

Has Abstract

pub_date

2015-07-08 00:00:00

pages

12030

issn

2045-2322

pii

srep12030

journal_volume

5

pub_type

杂志文章
  • Riparian plant litter quality increases with latitude.

    abstract::Plant litter represents a major basal resource in streams, where its decomposition is partly regulated by litter traits. Litter-trait variation may determine the latitudinal gradient in decomposition in streams, which is mainly microbial in the tropics and detritivore-mediated at high latitudes. However, this hypothes...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10640-3

    authors: Boyero L,Graça MAS,Tonin AM,Pérez J,J Swafford A,Ferreira V,Landeira-Dabarca A,A Alexandrou M,Gessner MO,McKie BG,Albariño RJ,Barmuta LA,Callisto M,Chará J,Chauvet E,Colón-Gaud C,Dudgeon D,Encalada AC,Figueroa R,Fle

    更新日期:2017-09-05 00:00:00

  • Macroalgal browsing on a heavily degraded, urbanized equatorial reef system.

    abstract::The removal of macroalgal biomass is critical to the health of coral reef ecosystems. Previous studies on relatively intact reefs with diverse and abundant fish communities have quantified rapid removal of macroalgae by herbivorous fishes, yet how these findings relate to degraded reef systems where fish diversity and...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08873-3

    authors: Bauman AG,Hoey AS,Dunshea G,Feary DA,Low J,Todd PA

    更新日期:2017-08-21 00:00:00

  • Progress towards non-invasive diagnosis and follow-up of celiac disease in children; a prospective multicentre study to the usefulness of plasma I-FABP.

    abstract::This prospective study investigates whether measurement of plasma intestinal-fatty acid binding protein (I-FABP), a sensitive marker for small intestinal epithelial damage, improves non-invasive diagnosing of celiac disease (CD), and whether I-FABP levels are useful to evaluate mucosal healing in patients on a gluten-...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/s41598-017-07242-4

    authors: Adriaanse MPM,Mubarak A,Riedl RG,Ten Kate FJW,Damoiseaux JGMC,Buurman WA,Houwen RHJ,Vreugdenhil ACE,Celiac Disease Study Group.

    更新日期:2017-08-17 00:00:00

  • 15-Lipoxygenase metabolites of α-linolenic acid, [13-(S)-HPOTrE and 13-(S)-HOTrE], mediate anti-inflammatory effects by inactivating NLRP3 inflammasome.

    abstract::The ratio of ω-6 to ω-3 polyunsaturated fatty acids (PUFAs) appears to be critical in the regulation of various pathophysiological processes and to maintain cellular homeostasis. While a high proportion of dietary intake of ω-6 PUFAs is associated with various inflammatory disorders, higher intake of ω-3 PUFAs is know...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31649

    authors: Kumar N,Gupta G,Anilkumar K,Fatima N,Karnati R,Reddy GV,Giri PV,Reddanna P

    更新日期:2016-08-18 00:00:00

  • Asymptotic entropy of the Gibbs state of complex networks.

    abstract::In this work we study the entropy of the Gibbs state corresponding to a graph. The Gibbs state is obtained from the Laplacian, normalized Laplacian or adjacency matrices associated with a graph. We calculated the entropy of the Gibbs state for a few classes of graphs and studied their behavior with changing graph orde...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78626-2

    authors: Glos A,Krawiec A,Pawela Ł

    更新日期:2021-01-11 00:00:00

  • Hierarchically-structured silver nanoflowers for highly conductive metallic inks with dramatically reduced filler concentration.

    abstract::Silver has long been employed as an electrically conductive component, and morphology-dependent properties have been actively investigated. Here we present a novel scalable synthesis method of flower-shaped silver nanoparticles (silver nanoflowers, Ag NFs). The preferential affinity of citrate molecules on (111) surfa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34894

    authors: C MA,K P F,Singh S,Baik S

    更新日期:2016-10-07 00:00:00

  • Ingestion of genetically modified yeast symbiont reduces fitness of an insect pest via RNA interference.

    abstract::RNA interference has had major advances as a developing tool for pest management. In laboratory experiments, double-stranded RNA (dsRNA) is often administered to the insect by genetic modification of the crop, or synthesized in vitro and topically applied to the crop. Here, we engineered genetically modified yeast tha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22587

    authors: Murphy KA,Tabuloc CA,Cervantes KR,Chiu JC

    更新日期:2016-03-02 00:00:00

  • Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots.

    abstract::The biological function of sterol glucosides (SGs), the most abundant sterol derivatives in higher plants, remains uncertain. In an effort to improve our understanding of these membrane lipids we examined phenotypes exhibited by the roots of Arabidopsis (Arabidopsis thaliana) lines carrying insertions in the UDP-Glc:s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05925-6

    authors: Pook VG,Nair M,Ryu K,Arpin JC,Schiefelbein J,Schrick K,DeBolt S

    更新日期:2017-07-18 00:00:00

  • An agent-based algorithm resembles behaviour of tree-dwelling bats under fission-fusion dynamics.

    abstract::Utilization of computational approach in the study of social behaviour of animals is increasing and we attempted such an approach in our study of tree-dwelling bats. These bats live in highly dynamic fission-fusion societies that share multiple roosts in a common home range. The key behavioural component associated wi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72999-0

    authors: Zelenka J,Kasanický T,Budinská I,Kaňuch P

    更新日期:2020-10-08 00:00:00

  • Hybrid Harris hawks optimization with cuckoo search for drug design and discovery in chemoinformatics.

    abstract::One of the major drawbacks of cheminformatics is a large amount of information present in the datasets. In the majority of cases, this information contains redundant instances that affect the analysis of similarity measurements with respect to drug design and discovery. Therefore, using classical methods such as the p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71502-z

    authors: Houssein EH,Hosney ME,Elhoseny M,Oliva D,Mohamed WM,Hassaballah M

    更新日期:2020-09-02 00:00:00

  • An update to the Monro-Kellie doctrine to reflect tissue compliance after severe ischemic and hemorrhagic stroke.

    abstract::High intracranial pressure (ICP) can impede cerebral blood flow resulting in secondary injury or death following severe stroke. Compensatory mechanisms include reduced cerebral blood and cerebrospinal fluid volumes, but these often fail to prevent raised ICP. Serendipitous observations in intracerebral hemorrhage (ICH...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78880-4

    authors: Kalisvaart ACJ,Wilkinson CM,Gu S,Kung TFC,Yager J,Winship IR,van Landeghem FKH,Colbourne F

    更新日期:2020-12-16 00:00:00

  • Linear response of a superfluid Fermi gas inside its pair-breaking continuum.

    abstract::We study the signatures of the collective modes of a superfluid Fermi gas in its linear response functions for the order-parameter and density fluctuations in the Random Phase Approximation (RPA). We show that a resonance associated to the Popov-Andrianov (or sometimes "Higgs") mode is visible inside the pair-breaking...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-65371-9

    authors: Kurkjian H,Tempere J,Klimin SN

    更新日期:2020-07-14 00:00:00

  • Using epidemic simulators for monitoring an ongoing epidemic.

    abstract::Prediction of infection trends, estimating the efficacy of contact tracing, testing or impact of influx of infected are of vital importance for administration during an ongoing epidemic. Most effective methods currently are empirical in nature and their relation to parameters of interest to administrators are not evid...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73308-5

    authors: Raghavan M,Sridharan KS,Mandayam Rangayyan Y

    更新日期:2020-10-06 00:00:00

  • Compact wideband plasmonic filter with flat-top transmission response based on corrugated metal-insulator-metal ring resonator.

    abstract:ABSTARCT:We demonstrate a novel route to control the filtering of spoof localized surface plasmons (LSPs) on the corrugated metal-insulator-metal (MIM) ring resonator. The spoof LSPs resonance modes can be effectively tuned to achieve broad passband (covering the quadrupole mode and the hexapole mode) by selecting prop...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14708-y

    authors: Yang L,Zhou YJ,Zhang C,Xiao QX

    更新日期:2017-10-27 00:00:00

  • Hypocretin in median raphe nucleus modulates footshock stimuli-induced REM sleep alteration.

    abstract::Stress is one of major factors that cause sleep problems. Hypocretin represents a stress-related neuropeptide and is well known in maintaining physiological wakefulness. The hypocretinergic neurons originate in the lateral hypothalamic area (LHA) and transmit to several brain regions, including the median raphe nuclei...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44731-0

    authors: Hsiao YT,Lo Y,Yi PL,Chang FC

    更新日期:2019-06-03 00:00:00

  • Differential effects of an exotic plant virus on its two closely related vectors.

    abstract::Concurrent spread of Tomato yellow leaf curl virus (TYLCV) with invasion of Bemisia tabaci Q rather than B in China suggests a more mutualistic relationship between TYLCV and Q than B. To assess this hypothesis, we quantified the impacts of TYLCV on the performance and competitiveness of B and Q in the laboratory. The...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02230

    authors: Pan H,Chu D,Liu B,Shi X,Guo L,Xie W,Carrière Y,Li X,Zhang Y

    更新日期:2013-01-01 00:00:00

  • The development of cross-cultural recognition of vocal emotion during childhood and adolescence.

    abstract::Humans have an innate set of emotions recognised universally. However, emotion recognition also depends on socio-cultural rules. Although adults recognise vocal emotions universally, they identify emotions more accurately in their native language. We examined developmental trajectories of universal vocal emotion recog...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26889-1

    authors: Chronaki G,Wigelsworth M,Pell MD,Kotz SA

    更新日期:2018-06-14 00:00:00

  • Polymorphism in the gene encoding toll-like receptor 10 may be associated with asthma after bronchiolitis.

    abstract::Toll-like receptors (TLRs) recognise microbes that contribute to the severity of bronchiolitis and the subsequent risk of asthma. We evaluated whether post-bronchiolitis asthma was associated with polymorphisms in the TLR3 rs3775291, TLR4 rs4986790, TLR7 rs179008, TLR8 rs2407992, TLR9 rs187084, and TLR10 rs4129009 gen...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03429-x

    authors: Törmänen S,Korppi M,Teräsjärvi J,Vuononvirta J,Koponen P,Helminen M,He Q,Nuolivirta K

    更新日期:2017-06-07 00:00:00

  • Hard plant tissues do not contribute meaningfully to dental microwear: evolutionary implications.

    abstract::Reconstructing diet is critical to understanding hominin adaptations. Isotopic and functional morphological analyses of early hominins are compatible with consumption of hard foods, such as mechanically-protected seeds, but dental microwear analyses are not. The protective shells surrounding seeds are thought to induc...

    journal_title:Scientific reports

    pub_type: 历史文章,杂志文章

    doi:10.1038/s41598-019-57403-w

    authors: van Casteren A,Strait DS,Swain MV,Michael S,Thai LA,Philip SM,Saji S,Al-Fadhalah K,Almusallam AS,Shekeban A,McGraw WS,Kane EE,Wright BW,Lucas PW

    更新日期:2020-01-17 00:00:00

  • Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer.

    abstract::Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edg...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29935

    authors: Hiraide T,Ikegami K,Sakaguchi T,Morita Y,Hayasaka T,Masaki N,Waki M,Sugiyama E,Shinriki S,Takeda M,Shibasaki Y,Miyazaki S,Kikuchi H,Okuyama H,Inoue M,Setou M,Konno H

    更新日期:2016-07-20 00:00:00

  • Nasal commensal Staphylococcus epidermidis counteracts influenza virus.

    abstract::Several microbes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside the human nasal cavity as commensals. The role of these nasal commensals in host innate immunity is largely unknown, although bacterial interference in the nasal microbiome may promote ecological competition...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27870

    authors: Chen HW,Liu PF,Liu YT,Kuo S,Zhang XQ,Schooley RT,Rohde H,Gallo RL,Huang CM

    更新日期:2016-06-16 00:00:00

  • Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging.

    abstract::We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01833-x

    authors: Kim Y,Kim C,Kwon OY,Nam D,Kim SS,Park JH,Kim S,Gallagher-Jones M,Kohmura Y,Ishikawa T,Song C,Tae G,Noh DY

    更新日期:2017-05-12 00:00:00

  • hnRNPK inhibits GSK3β Ser9 phosphorylation, thereby stabilizing c-FLIP and contributes to TRAIL resistance in H1299 lung adenocarcinoma cells.

    abstract::c-FLIP (cellular FLICE-inhibitory protein) is the pivotal regulator of TRAIL resistance in cancer cells, It is a short-lived protein degraded through the ubiquitin/proteasome pathway. The discovery of factors and mechanisms regulating its protein stability is important for the comprehension of TRAIL resistance by tumo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22999

    authors: Gao X,Feng J,He Y,Xu F,Fan X,Huang W,Xiong H,Liu Q,Liu W,Liu X,Sun X,He QY,Zhang Q,Liu L

    更新日期:2016-03-14 00:00:00

  • Repeated superovulation may affect mitochondrial functions of cumulus cells in mice.

    abstract::Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31368

    authors: Xie JK,Wang Q,Zhang TT,Yin S,Zhang CL,Ge ZJ

    更新日期:2016-10-04 00:00:00

  • The capacity of wastewater treatment plants drives bacterial community structure and its assembly.

    abstract::Bacterial communities in wastewater treatment plants (WWTPs) affect plant functionality through their role in the removal of pollutants from wastewater. Bacterial communities vary extensively based on plant operating conditions and influent characteristics. The capacity of WWTPs can also affect the bacterial community...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50952-0

    authors: Kim YK,Yoo K,Kim MS,Han I,Lee M,Kang BR,Lee TK,Park J

    更新日期:2019-10-15 00:00:00

  • Impact of microscopic In fluctuations on the optical properties of InxGa1-xN blue light-emitting diodes assessed by low-energy X-ray fluorescence mapping using synchrotron radiation.

    abstract::Among the III-nitride semiconductors, InxGa1-xN is a key material for visible optical devices such as light-emitting diodes (LEDs), laser diodes, and solar cells. Light emission is achieved via electron-hole recombination within the InxGa1-xN layer. When InxGa1-xN-based blue LEDs were first commercialized, the high pr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39086-5

    authors: Sakaki A,Funato M,Miyano M,Okazaki T,Kawakami Y

    更新日期:2019-03-06 00:00:00

  • Histone Variant MacroH2A1 Plays an Isoform-Specific Role in Suppressing Epithelial-Mesenchymal Transition.

    abstract::Epithelial-Mesenchymal Transition (EMT) is a biological program that plays key roles in various developmental and pathological processes. Although much work has been done on signaling pathways and transcription factors regulating EMT, the epigenetic regulation of EMT remains not well understood. Histone variants have ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19364-4

    authors: Hodge DQ,Cui J,Gamble MJ,Guo W

    更新日期:2018-01-16 00:00:00

  • Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.

    abstract::Hereditary Parkinson's disease is commonly caused by mutations in the protein kinase PINK1 or the E3 ubiquitin ligase Parkin, which function together to eliminate damaged mitochondria. PINK1 phosphorylates both Parkin and ubiquitin to stimulate ubiquitination of dozens of proteins on the surface of the outer mitochond...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33019

    authors: Klosowiak JL,Park S,Smith KP,French ME,Focia PJ,Freymann DM,Rice SE

    更新日期:2016-09-08 00:00:00

  • Quantifying the functional disparity in pigment spot-background egg colour ICP-OES-based eggshell ionome at two extremes of avian embryonic development.

    abstract::It is known that a developing avian embryo resorbs micronutrients (calcium and other chemical elements) from the inner layer of the eggshell, inducing thinning and overall changes in the shell's chemical composition. However, an aspect yet to be explored relates to the local changes in the multi-elemental composition ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79040-4

    authors: Orłowski G,Niedzielski P,Merta D,Pokorny P,Proch J

    更新日期:2020-12-17 00:00:00

  • Longitudinal assessment of HLA and MIC-A antibodies in uneventful pregnancies and pregnancies complicated by preeclampsia or gestational diabetes.

    abstract::The significance of antibodies directed against paternal epitopes in the context of obstetric disorders is discussed controversially. In this study anti-HLA and anti-MIC-A antibodies were analysed in sera of women with uneventful pregnancy (n = 101), preeclampsia (PE, n = 55) and gestational diabetes (GDM, n = 36) usi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-13275-6

    authors: Küssel L,Herkner H,Wahrmann M,Eskandary F,Doberer K,Binder J,Pateisky P,Zeisler H,Böhmig GA,Bond G

    更新日期:2017-10-19 00:00:00