The microbiomes of blowflies and houseflies as bacterial transmission reservoirs.

Abstract:

:Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Junqueira ACM,Ratan A,Acerbi E,Drautz-Moses DI,Premkrishnan BNV,Costea PI,Linz B,Purbojati RW,Paulo DF,Gaultier NE,Subramanian P,Hasan NA,Colwell RR,Bork P,Azeredo-Espin AML,Bryant DA,Schuster SC

doi

10.1038/s41598-017-16353-x

subject

Has Abstract

pub_date

2017-11-24 00:00:00

pages

16324

issue

1

issn

2045-2322

pii

10.1038/s41598-017-16353-x

journal_volume

7

pub_type

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