Abstract：
:The aim of this study was to develop a novel method to detect circulating histones H3 and H2B in plasma based on multiple reaction monitoring targeted mass spectrometry and a multiple reaction monitoring approach (MRM-MS) for its clinical application in critical bacteriaemic septic shock patients. Plasma samples from 17 septic shock patients with confirmed bacteraemia and 10 healthy controls were analysed by an MRM-MS method, which specifically detects presence of histones H3 and H2B. By an internal standard, it was possible to quantify the concentration of circulating histones in plasma, which were significantly higher in patients, and thus confirmed their potential as biomarkers for diagnosing septic shock. After comparing surviving patients and non-survivors, a correlation was found between higher levels of circulating histones and unfavourable outcome. Indeed, histone H3 proved a more efficient and sensitive biomarker for septic shock prognosis. In conclusion, these findings suggest the accuracy of the MRM-MS technique and stable isotope labelled peptides to detect and quantify circulating plasma histones H2B and H3. This method may be used for early septic shock diagnoses and for the prognosis of fatal outcomes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
García-Giménez JL,Romá-Mateo C,Carbonell N,Palacios L,Peiró-Chova L,García-López E,García-Simón M,Lahuerta R,Gimenez-Garzó C,Berenguer-Pascual E,Mora MI,Valero ML,Alpízar A,Corrales FJ,Blanquer J,Pallardó FVdoi
10.1038/s41598-017-10830-zsubject
Has Abstractpub_date
2017-09-06 00:00:00pages
10643issue
1issn
2045-2322pii
10.1038/s41598-017-10830-zjournal_volume
7pub_type
杂志文章abstract：:Lead (Pb) toxicity is a major environmental concern affirming the need of proper mitigation strategies. In the present work, potential of combined treatment of 24-Epibrassinolide (24-EBL) and Salicylic acid (SA) against Pb toxicity to Brassica juncea L. seedlings were evaluated. Seedlings pre-imbibed in EBL (0.1 mM) a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39712-2
更新日期：2019-03-05 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are multi-potent cells that are self-renewable and possess the potential to differentiate into multiple lineages. Several studies demonstrated that MSCs could regulate a Th17/Treg balance and could be a potential therapeutic target for Rheumatoid Arthritis (RA). A20 is highly expressed in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18693-0
更新日期：2018-01-11 00:00:00
abstract：:A large volcanic sulfate increase observed in ice core records around 1450 C.E. has been attributed in previous studies to a volcanic eruption from the submarine Kuwae caldera in Vanuatu. Both EPMA-WDS (electron microprobe analysis using a wavelength dispersive spectrometer) and SEM-EDS (scanning electron microscopy a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50939-x
更新日期：2019-10-08 00:00:00
abstract：:Detrimental impacts of extreme heats on the U.S. crop yields have been well-documented by a number of empirical studies. However, less have focused on within-growing season weather variation and the interaction between temperature and precipitation. The objective of this study is to emphasize the importance of disaggr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55037-6
更新日期：2019-12-06 00:00:00
abstract：:Iodinated contrast agent (ICA)-induced acute kidney injury (AKI) following acute coronary syndrome (ACS) is a frequent complication, which may lead to chronic kidney disease and increased mortality. Optical coherence tomography angiography (OCT-A) of the retina is new tool delivering a rapid and noninvasive assessment...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44647-9
更新日期：2019-05-30 00:00:00
abstract：:Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51204-x
更新日期：2019-10-16 00:00:00
abstract：:Electroencephalographic hyperscanning was used to investigate interbrain synchronization patterns in dyads of participants interacting through speech. Results show that brain oscillations are synchronized between listener and speaker during oral narratives. This interpersonal synchronization is mediated in part by a l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04464-4
更新日期：2017-06-23 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-28651-z
更新日期：2018-07-05 00:00:00
abstract：:E-mail has become the main carrier of spreading malicious software and been widely used for phishing, even high-level persistent threats. The e-mail accounts with high social reputation are primary targets to be attacked and utilized by attackers, suffering a lot of probing attacks for a long time. In this paper, in o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63191-5
更新日期：2020-04-29 00:00:00
abstract：:Recent stroke studies have shown that the ipsi-lesional thalamus longitudinally and significantly decreases after stroke in the acute and subacute stages. However, additional considerations in the chronic stages of stroke require exploration including time since stroke, gender, intracortical volume, aging, and lesion ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76382-x
更新日期：2020-11-24 00:00:00
abstract：:Circular RNA (circRNA) is a newly discovered non-coding RNA, which play significant roles in the function and transcriptional regulation of microRNA. To date, in Chinese cabbage, the functional characteristic of circRNAs in response to calcium deficiency-induced tip-burn have not been reported. In this study, 730 circ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51190-0
更新日期：2019-10-10 00:00:00
abstract：:The association between cervical lymph node metastasis (LNM) and ultrasonographic features as well as BRAFV600E mutations in patients with papillary thyroid carcinoma (PTC) remained controversial. This study investigated the association between LNM and ultrasonographic features as well as BRAFV600E mutation in Chinese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36171-z
更新日期：2019-01-11 00:00:00
abstract：:Hydrogen in the Earth's deep interior has been thought to exist as a hydroxyl group in high-pressure minerals. We present Muon Spin Rotation experiments on SiO2 stishovite, which is an archetypal high-pressure mineral. Positive muon (which can be considered as a light isotope of proton) implanted in stishovite was fou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08437
更新日期：2015-02-13 00:00:00
abstract：:Hearing impairment is one of the most common sensory deficits in humans. Hearing aids are helpful to patients but can have poor sound quality or transmission due to insufficient output or acoustic feedback, such as for high frequencies. Implantable devices partially overcome these issues but require surgery with limit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40860-8
更新日期：2019-03-12 00:00:00
abstract：:A practical quantum computer must be capable of performing high fidelity quantum gates on a set of quantum bits (qubits). In the presence of noise, the realization of such gates poses daunting challenges. Geometric phases, which possess intrinsic noise-tolerant features, hold the promise for performing robust quantum ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18414
更新日期：2015-12-21 00:00:00
abstract：:In solid-liquid systems, macroscopic solids lose their equilibrium and melt in a manner that results in overall movement of the solid-liquid interface. This phenomenon occurs when they are subjected to temperature gradients or external stress, for example. However, many experiments suggest that the melting of nano- an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18466
更新日期：2015-12-21 00:00:00
abstract：:Voltage-gated potassium (Kv) channels comprise pore-forming α subunits and a multiplicity of regulatory proteins, including the cardiac-expressed and cardiac arrhythmia-linked transmembrane KCNE subunits. After recently uncovering novel, N-terminally extended (L) KCNE3 and KCNE4 isoforms and detecting their transcript...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38412
更新日期：2016-12-06 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-67554-w
更新日期：2020-06-25 00:00:00
abstract：:The pollination syndrome concept has provided powerful utility in understanding the evolution and adaptation of floral traits. However, the utility of this conception has been questioned on the grounds that flowers usually attract a broader spectrum of visitors than one might expect. Furthermore, the relationship betw...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70954-7
更新日期：2020-08-18 00:00:00
abstract：:The elastic modulus of ZnO nanowires was measured using a resonance method based on laser Doppler effect and their fracture strains were determined via two-point bending with the aid of optical nanomanipulation. The elastic moduli of ZnO nanowires with diameters of 78 to 310 nm vary from 123 to 154 GPa, which are clos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09843-5
更新日期：2017-08-25 00:00:00
abstract：:In this paper, we investigate the relationship between the quantum speedup, non-Markovianity and formation of a system-environment bound state. Previous results show a monotonic relation between these three such that providing bound states with more negative energy can lead to a higher degree of non-Markovianity, and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51290-x
更新日期：2019-10-18 00:00:00
abstract：:Competing phenomena in low dimensional systems can generate exotic electronic phases, either through symmetry breaking or a non-trivial topology. In two-dimensional (2D) systems, the interplay between superfluidity, disorder and repulsive interactions is especially fruitful in this respect although both the exact natu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35834
更新日期：2016-10-27 00:00:00
abstract：:Beta oscillations observed in motor cortical local field potentials (LFPs) recorded on separate electrodes of a multi-electrode array have been shown to exhibit non-zero phase shifts that organize into planar waves. Here, we generalize this concept to additional classes of salient patterns that fully describe the spat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22990-7
更新日期：2018-03-26 00:00:00
abstract：:Treponema pallidum subsp. pallidum, the causative agent of syphilis, is a highly invasive spirochete pathogen that uses the vasculature to disseminate throughout the body. Identification of bacterial factors promoting dissemination is crucial for syphilis vaccine development. An important step in dissemination is bact...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01589-4
更新日期：2017-05-08 00:00:00
abstract：:MALDI-TOF spectrometry has not been used for urinary exosome analysis. We used it for determining UC biomarkers. From 2012 to 2015, we enrolled 129 consecutive patients with UC and 62 participants without UC. Exosomes from their urine were isolated, and analyzed through MALDI-TOF spectrometry. Immunohistochemical (IHC...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34446
更新日期：2016-09-30 00:00:00
abstract：:Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni-Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19742
更新日期：2016-01-28 00:00:00
abstract：:Laser-Stimulated Fluorescence (LSF) is used to identify fully fledged feathering in the hatchling enantiornithine bird specimen MPCM-LH-26189, supporting precocial nesting behavior in this extinct group. The LSF results include the detection of a long pennaceous wing feather as well as cover feathers around the body. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41423-7
更新日期：2019-03-21 00:00:00
abstract：:Prediction of infection trends, estimating the efficacy of contact tracing, testing or impact of influx of infected are of vital importance for administration during an ongoing epidemic. Most effective methods currently are empirical in nature and their relation to parameters of interest to administrators are not evid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73308-5
更新日期：2020-10-06 00:00:00
abstract：:The chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. Considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. Mas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27974
更新日期：2016-06-13 00:00:00
abstract：:Cervical carcinoma is the fourth most common cause of death in woman, caused by human papillomavirus (HPV) infections and arising from the cervix. Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein, has been linked to tumorigenic effects. In the present study, we s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01832-y
更新日期：2017-05-18 00:00:00
