Abstract：
:Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni-Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distributed under various anoxic conditions. Ancestral sequences of [NiFeSe] Hases were elucidated and their three-dimensional structures were recreated in silico using homology modelling and molecular dynamic simulation, which suggested that deep gas channels gradually developed in [NiFeSe] Hases under absolute anaerobic conditions, whereas the enzyme remained as a sealed edifice under environmental conditions of a higher oxygen exposure risk. The development of a gas cavity appears to be driven by non-synonymous mutations, which cause subtle conformational changes locally and distantly, even including highly conserved sequence regions.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tamura T,Tsunekawa N,Nemoto M,Inagaki K,Hirano T,Sato Fdoi
10.1038/srep19742subject
Has Abstractpub_date
2016-01-28 00:00:00pages
19742issn
2045-2322pii
srep19742journal_volume
6pub_type
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